FastQCFastQC Report
Fri 27 May 2016
SRR2138628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6585276
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105200.049.195411
GTATCAA190200.036.473561
ATCAACG230950.029.4681033
TCAACGC234700.029.0332264
TATCAAC237300.029.0194872
CAACGCA237050.028.7078385
AACGCAG242650.028.1270166
CTTATAC49050.025.880271
ACGCAGA273750.024.8385527
CGCAGAG276050.024.6162288
GTGGTAT52650.024.1913171
TGGTATC51600.022.7911742
GCAGAGT315750.021.4001889
GTACATG229850.021.2786751
TACATGG225350.021.251432
TATACAC69950.021.1216663
ACATGGG231150.020.0386053
GAGTACT189100.019.96513712-13
CAGAGTA310500.019.02810310-11
AGAGTAC288550.018.46037510-11
Produced by FastQC (version 0.11.5)