##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138628_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6585276
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82036090818365	33.0	33.0	33.0	33.0	33.0
2	32.816916557483694	33.0	33.0	33.0	33.0	33.0
3	32.81314313933083	33.0	33.0	33.0	33.0	33.0
4	36.76226144507839	37.0	37.0	37.0	37.0	37.0
5	36.777498771501755	37.0	37.0	37.0	37.0	37.0
6	36.784098494884645	37.0	37.0	37.0	37.0	37.0
7	36.770429819494275	37.0	37.0	37.0	37.0	37.0
8	36.768594512971056	37.0	37.0	37.0	37.0	37.0
9	36.766972713064725	37.0	37.0	37.0	37.0	37.0
10-11	36.744283459038016	37.0	37.0	37.0	37.0	37.0
12-13	36.72710354129425	37.0	37.0	37.0	37.0	37.0
14-15	39.042479237013	40.0	40.0	40.0	37.0	40.0
16-17	38.99246880464843	40.0	40.0	40.0	37.0	40.0
18-19	39.02626700839873	40.0	40.0	40.0	37.0	40.0
20-21	39.04327994813538	40.0	40.0	40.0	37.0	40.0
22-23	39.07562561345731	40.0	40.0	40.0	37.0	40.0
24-25	39.07837625433508	40.0	40.0	40.0	37.0	40.0
26-27	38.99065022221565	40.0	40.0	40.0	37.0	40.0
28-29	38.91787274475749	40.0	40.0	40.0	37.0	40.0
30-31	38.84035423169027	40.0	38.5	40.0	37.0	40.0
32-33	38.80907196876022	40.0	37.0	40.0	37.0	40.0
34-35	38.78430186503236	40.0	37.0	40.0	37.0	40.0
36-37	38.750038352874704	40.0	37.0	40.0	37.0	40.0
38-39	38.699049635723455	40.0	37.0	40.0	37.0	40.0
40-41	38.66057589585817	40.0	37.0	40.0	37.0	40.0
42-43	38.59620898351146	40.0	37.0	40.0	37.0	40.0
44-45	38.506315455048735	40.0	37.0	40.0	37.0	40.0
46-47	38.41889826936033	40.0	37.0	40.0	37.0	40.0
48-49	38.37705014227956	40.0	37.0	40.0	37.0	40.0
50-51	38.31601100647151	40.0	37.0	40.0	37.0	40.0
52-53	38.24805596026	40.0	37.0	40.0	37.0	40.0
54-55	38.18201562276898	40.0	37.0	40.0	37.0	40.0
56-57	38.06705590786727	40.0	37.0	40.0	37.0	40.0
58-59	37.94942225727312	40.0	37.0	40.0	35.0	40.0
60-61	37.77712935201095	40.0	37.0	40.0	33.0	40.0
62-63	37.58814190945932	38.5	37.0	40.0	33.0	40.0
64-65	37.36631950310466	37.0	37.0	40.0	33.0	40.0
66-67	37.13539601473845	37.0	37.0	40.0	33.0	40.0
68-69	36.89445443599075	37.0	37.0	40.0	33.0	40.0
70-71	36.63319025620656	37.0	37.0	40.0	33.0	40.0
72-73	36.38699130162835	37.0	37.0	37.0	33.0	40.0
74-75	36.146291089674975	37.0	37.0	37.0	33.0	40.0
76-77	34.52735377547431	35.0	33.0	37.0	30.0	37.0
78-79	35.7627941361188	37.0	35.0	37.0	33.0	37.0
80-81	35.877538047795255	37.0	37.0	37.0	33.0	37.0
82-83	35.80876668034272	37.0	37.0	37.0	33.0	37.0
84-85	35.68940756740526	37.0	37.0	37.0	33.0	37.0
86-87	35.57938225881023	37.0	37.0	37.0	33.0	37.0
88-89	35.50532179881327	37.0	35.0	37.0	33.0	37.0
90-91	35.43632045349152	37.0	33.0	37.0	33.0	37.0
92-93	35.364330768278684	37.0	33.0	37.0	33.0	37.0
94-95	35.32392064524231	37.0	33.0	37.0	33.0	37.0
96-97	35.289019799340785	37.0	33.0	37.0	33.0	37.0
98-99	35.248424129865654	37.0	33.0	37.0	33.0	37.0
100	35.18764266321224	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	25.0
21	92.0
22	380.0
23	1246.0
24	2841.0
25	5794.0
26	10701.0
27	17468.0
28	26395.0
29	37327.0
30	52146.0
31	71807.0
32	96859.0
33	133626.0
34	192326.0
35	298402.0
36	600043.0
37	1877350.0
38	3030746.0
39	129697.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.87411431198935	20.116347439348022	14.493333309036704	23.51620493962592
2	14.637564165875508	20.575568890354788	41.9992571306047	22.787609813165005
3	18.360615657490218	25.998492062829197	30.215249299371084	25.425642980309497
4	12.449963573283917	18.024914142450406	38.43795376776173	31.087168516503954
5	13.396749341068281	37.59218741029583	35.199924302969166	13.811138945666727
6	29.934219917282128	37.47991731857556	18.688510549899505	13.897352214242806
7	26.591110228333637	32.72860545252773	22.58209071267476	18.098193606463873
8	24.62871715627409	36.421586581944325	20.86787250830489	18.081823753476694
9	25.550607142358196	17.10547895031279	21.216513931990093	36.127399975338925
10-11	24.439514759897687	26.7771540630947	28.20546169970704	20.577869477300574
12-13	25.3430835700736	24.308145930405956	28.533291543133497	21.815478956386947
14-15	22.40660831831498	25.67146768032198	26.46918671290315	25.452737288459893
16-17	21.68834685136963	28.88231260162824	27.14212737628613	22.287213170716
18-19	21.60133455302405	27.793064406108414	29.276206494610097	21.329394546257436
20-21	22.48158921571084	26.924467000296882	29.180381267810002	21.41356251618228
22-23	22.31741109748341	26.9181797361977	29.032786666156383	21.7316225001625
24-25	22.05005763939597	27.12052444027354	29.138077987036947	21.691339933293545
26-27	21.943608191085815	27.34900127154424	29.074280832667664	21.63310970470228
28-29	21.71387886124669	27.31653497944328	29.189308908180543	21.780277251129483
30-31	22.07636733308264	27.262272766334284	29.02867226120467	21.63268763937841
32-33	22.07606532098466	27.214407716074714	28.994265234342947	21.71526172859768
34-35	21.994827580003047	27.312891752707152	29.096290291870957	21.59599037541884
36-37	21.916496244744977	27.392417324828656	28.89850287357007	21.7925835568563
38-39	21.87900205827879	27.265267528260708	29.03062312695547	21.82510728650504
40-41	22.05757036746743	27.129441938708947	28.93111629327713	21.88187140054649
42-43	22.015124764779983	27.234362384903193	28.756247663507363	21.994265186809457
44-45	22.05969795304863	27.539246748400576	28.458796799229972	21.942258499320822
46-47	22.249648156070286	27.403930447717844	27.97537096496827	22.371050431243596
48-49	22.257522460451863	27.511544623313306	27.979259521255205	22.251673394979623
50-51	22.14965509095995	27.70111817410632	27.809268276000036	22.339958458933694
52-53	22.21994793172796	27.764564863007134	27.770397759970123	22.245089445294788
54-55	22.2596901667236	27.695925934524425	27.663572866538207	22.38081103221377
56-57	22.406343913658976	27.881209173064725	27.455946716584467	22.25650019669183
58-59	22.18550937541878	28.061268578600167	27.708262095183056	22.044959950797995
60-61	22.437742146986945	28.114714873253348	27.380086683299414	22.06745629646029
62-63	22.313894887419387	28.17206769217342	27.344101537916504	22.16993588249069
64-65	22.26793278570807	28.10872861413084	27.339395559568374	22.283943040592714
66-67	22.359521395694674	27.93931748581159	27.31771230810808	22.38344881038565
68-69	22.34407517117948	27.81104352480874	27.47258264452583	22.372298659485956
70-71	22.343682420080594	27.62434526779008	27.4817386765434	22.55023363558593
72-73	22.407628890171797	27.685607822022074	27.477404359036743	22.429358928769382
74-75	22.356506352414034	27.829465658179025	27.38984265316862	22.42418533623832
76-77	22.41397247957508	27.74458733957239	27.308798324555667	22.532641856296863
78-79	22.332054755273848	27.763554774980015	27.335215587791627	22.569174881954513
80-81	22.39442446674829	27.783101747631534	27.38476819211678	22.437705593503395
82-83	22.449554144307903	27.742707635246404	27.514069718250195	22.293668502195498
84-85	22.638430916625644	27.722187238193268	27.333804399558577	22.30557744562251
86-87	22.321249725667194	27.832611342716707	27.428174618494193	22.417964313121907
88-89	22.49649660492245	27.695796022619025	27.375524300360638	22.43218307209789
90-91	22.46870438035567	27.776149447670317	27.360992054063992	22.394154117910016
92-93	22.491440923346527	27.767490787664332	27.407150745100704	22.333917543888433
94-95	22.315753386023705	27.804208082292668	27.47631673587995	22.40372179580368
96-97	22.39863348890137	27.904067519044002	27.523894463040023	22.173404529014608
98-99	23.47585436477416	29.824610785257477	27.717114453516057	18.982420396452305
100	22.438778448689085	30.778452369232863	21.5099322576876	25.27283692439045
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1853.0
1	1634.5
2	2102.5
3	2982.0
4	4291.5
5	7203.0
6	9478.0
7	10778.0
8	11469.5
9	11107.5
10	10082.5
11	9245.5
12	10352.0
13	14093.0
14	22445.5
15	33551.0
16	42551.0
17	47759.5
18	48211.0
19	44633.0
20	39377.5
21	33939.0
22	30717.5
23	31377.0
24	35498.5
25	42829.5
26	52739.5
27	63986.5
28	76359.5
29	90848.0
30	104381.5
31	117006.0
32	132626.0
33	147309.5
34	161094.0
35	175823.0
36	190740.5
37	205667.0
38	216281.0
39	222687.5
40	230102.0
41	237208.5
42	242871.0
43	249689.0
44	257199.0
45	267466.5
46	279096.0
47	285991.5
48	285628.5
49	280331.0
50	274188.5
51	266615.5
52	254463.0
53	240974.5
54	229059.5
55	213802.0
56	195551.0
57	176241.0
58	156926.0
59	136919.0
60	113224.0
61	90374.5
62	71698.0
63	56043.0
64	42247.5
65	31178.5
66	23388.5
67	17880.0
68	13544.0
69	9812.0
70	7001.5
71	4968.5
72	3360.5
73	2183.0
74	1447.5
75	974.5
76	616.5
77	365.5
78	246.5
79	185.0
80	122.0
81	79.5
82	63.5
83	44.0
84	24.0
85	13.0
86	9.0
87	6.0
88	4.0
89	3.5
90	4.0
91	3.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.001837432478152776
4	0.012497577929915162
5	0.01722023496053924
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.004139171240704048
26-27	0.04279464125699706
28-29	0.08944780442773853
30-31	0.08551939349204647
32-33	0.03525494399080624
34-35	0.0031081632385897297
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.4924509344318215E-4
56-57	3.177834105351891E-4
58-59	2.5316611655076017E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.675879780213938E-5
72-73	0.012946367401352774
74-75	0.02579266867661383
76-77	0.004995777835746694
78-79	0.0
80-81	0.0
82-83	0.005848425546486943
84-85	0.04259233518241693
86-87	0.03995926127937049
88-89	0.011168997570380399
90-91	1.4659836095213876E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	83.0
20-21	358.0
22-23	955.0
24-25	1835.0
26-27	3110.0
28-29	6628.0
30-31	15523.0
32-33	19686.0
34-35	26435.0
36-37	38349.0
38-39	45929.0
40-41	47128.0
42-43	55058.0
44-45	65168.0
46-47	63770.0
48-49	56888.0
50-51	49194.0
52-53	46548.0
54-55	50485.0
56-57	53859.0
58-59	52838.0
60-61	52535.0
62-63	51266.0
64-65	53941.0
66-67	58075.0
68-69	51492.0
70-71	49130.0
72-73	46260.0
74-75	46648.0
76-77	46566.0
78-79	49776.0
80-81	50002.0
82-83	48133.0
84-85	47794.0
86-87	50031.0
88-89	53570.0
90-91	56610.0
92-93	58101.0
94-95	70311.0
96-97	205789.0
98-99	391767.0
100-101	4347652.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.20756062890283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.2263557442262	73.41099844654849
2	8.091429007023528	13.465361393527461
3	2.0886526418053544	5.21375073977211
4	0.7650089096205417	2.546181009156082
5	0.3395857987538654	1.4128052969263323
6	0.17476074451961884	0.8724849147101027
7	0.0971097716213461	0.5656187046889443
8	0.06380919105536093	0.424752570673615
9	0.0365383581655069	0.27362408871032834
>10	0.11321441096397658	1.4122042853310706
>50	0.002287691529527023	0.1276862364844236
>100	0.0010637524896042298	0.1757677559192254
>500	1.839782256144104E-4	0.09876455755182635
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5185392381427901E-5
2	0.0	3.0370784762855803E-5	0.0	0.0	1.5185392381427901E-5
3	0.0	3.0370784762855803E-5	0.0	0.0	1.5185392381427901E-5
4	0.0	1.51853923814279E-4	0.0	0.0	1.5185392381427901E-5
5	0.0	1.51853923814279E-4	0.0	0.0	1.5185392381427901E-5
6	0.0	1.51853923814279E-4	0.0	1.5185392381427901E-5	1.5185392381427901E-5
7	0.0	1.51853923814279E-4	1.5185392381427901E-5	1.5185392381427901E-5	3.0370784762855803E-5
8	0.0	1.51853923814279E-4	1.5185392381427901E-5	1.5185392381427901E-5	4.55561771442837E-5
9	0.0	2.277808857214185E-4	1.5185392381427901E-5	1.5185392381427901E-5	6.0741569525711605E-5
10-11	0.0	2.277808857214185E-4	1.5185392381427901E-5	1.5185392381427901E-5	6.0741569525711605E-5
12-13	0.0	2.277808857214185E-4	1.5185392381427901E-5	3.796348095356975E-5	6.0741569525711605E-5
14-15	0.0	2.885224552471301E-4	1.5185392381427901E-5	4.55561771442837E-5	6.833426571642556E-5
16-17	0.0	3.03707847628558E-4	1.5185392381427901E-5	4.55561771442837E-5	9.11123542885674E-5
18-19	0.0	3.188932400099859E-4	1.5185392381427901E-5	4.55561771442837E-5	9.11123542885674E-5
20-21	0.0	3.188932400099859E-4	1.5185392381427901E-5	4.55561771442837E-5	9.11123542885674E-5
22-23	0.0	3.644494171542696E-4	1.5185392381427901E-5	5.314887333499765E-5	1.2148313905142321E-4
24-25	0.0	3.8722750572641146E-4	1.5185392381427901E-5	1.51853923814279E-4	1.7463201238642086E-4
26-27	0.0	3.948202019171254E-4	1.5185392381427901E-5	2.5055897429356034E-4	1.822247085771348E-4
28-29	0.0	3.948202019171254E-4	1.5185392381427901E-5	6.605645685921137E-4	1.822247085771348E-4
30-31	0.0	4.4037637906140913E-4	1.5185392381427901E-5	0.0018602105667249176	1.822247085771348E-4
32-33	0.0	4.4037637906140913E-4	1.5185392381427901E-5	0.004434134575376947	1.822247085771348E-4
34-35	0.0	5.846376066849742E-4	1.5185392381427901E-5	0.0072510248621318225	1.974101009585627E-4
36-37	0.0	7.440842266899671E-4	1.5185392381427901E-5	0.011434600463215209	2.125954933399906E-4
38-39	0.0	7.440842266899671E-4	1.5185392381427901E-5	0.017835243351987072	2.125954933399906E-4
40-41	0.0	8.959381505042461E-4	1.5185392381427901E-5	0.024326998595047496	2.125954933399906E-4
42-43	0.0	9.490870238392437E-4	1.5185392381427901E-5	0.030545416775242223	2.125954933399906E-4
44-45	0.0	0.0011161263400349506	1.5185392381427901E-5	0.03709032089163765	2.125954933399906E-4
46-47	0.0	0.00123001678289566	1.5185392381427901E-5	0.04420467722233662	2.125954933399906E-4
48-49	0.0	0.00123001678289566	1.5185392381427901E-5	0.05131903355303559	2.4296627810284642E-4
50-51	0.0	0.001366685314328511	1.5185392381427901E-5	0.058342277529446	2.4296627810284642E-4
52-53	0.0	0.001381870706709939	1.5185392381427901E-5	0.0650921844429907	2.657443666749883E-4
54-55	0.0	0.0013970560990913669	1.5185392381427901E-5	0.07159912507843255	2.9611515143784404E-4
56-57	0.0	0.0014274268838542228	1.5185392381427901E-5	0.07751383541099872	3.03707847628558E-4
58-59	0.0	0.0014274268838542228	1.5185392381427901E-5	0.0834437311359463	3.03707847628558E-4
60-61	0.0	0.0014577976686170784	1.5185392381427901E-5	0.08941159034184748	3.03707847628558E-4
62-63	0.0	0.0015185392381427902	1.5185392381427901E-5	0.09530352258584152	3.03707847628558E-4
64-65	0.0	0.0016400223771942132	1.5185392381427901E-5	0.10068674418505769	3.03707847628558E-4
66-67	0.0	0.0016476150733849273	1.5185392381427901E-5	0.10647237868238173	3.03707847628558E-4
68-69	0.0	0.0017007639467199248	1.5185392381427901E-5	0.11221245700256147	3.03707847628558E-4
70-71	0.0	0.0017159493391013527	1.5185392381427901E-5	0.1175121589436798	3.03707847628558E-4
72-73	0.0	0.0017159493391013527	1.5185392381427901E-5	0.12106554076093394	3.3407863239141383E-4
74-75	0.0	0.0017235420352920668	1.5185392381427901E-5	0.12402669227531238	3.3407863239141383E-4
76-77	0.0	0.0017842836048177783	1.5185392381427901E-5	0.12701062187826295	3.3407863239141383E-4
78-79	0.0	0.001837432478152776	1.5185392381427901E-5	0.12868101504022003	3.3407863239141383E-4
80-81	0.0	0.001837432478152776	1.5185392381427901E-5	0.12939472848214714	3.4167132858212775E-4
82-83	0.0	0.0018450251743434899	1.5185392381427901E-5	0.12989584643073426	3.568567209635557E-4
84-85	0.0	0.0018678032629156319	1.5185392381427901E-5	0.1300780711393114	3.644494171542696E-4
86-87	0.0	0.0018678032629156319	1.5185392381427901E-5	0.13016159079740924	3.644494171542696E-4
88	0.0	0.0020652133638741944	1.5185392381427901E-5	0.13016918349359996	3.644494171542696E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10520	0.0	49.19541	1
GTATCAA	19020	0.0	36.47356	1
ATCAACG	23095	0.0	29.468103	3
TCAACGC	23470	0.0	29.033226	4
TATCAAC	23730	0.0	29.019487	2
CAACGCA	23705	0.0	28.707838	5
AACGCAG	24265	0.0	28.127016	6
CTTATAC	4905	0.0	25.88027	1
ACGCAGA	27375	0.0	24.838552	7
CGCAGAG	27605	0.0	24.616228	8
GTGGTAT	5265	0.0	24.191317	1
TGGTATC	5160	0.0	22.791174	2
GCAGAGT	31575	0.0	21.400188	9
GTACATG	22985	0.0	21.278675	1
TACATGG	22535	0.0	21.25143	2
TATACAC	6995	0.0	21.121666	3
ACATGGG	23115	0.0	20.038605	3
GAGTACT	18910	0.0	19.965137	12-13
CAGAGTA	31050	0.0	19.028103	10-11
AGAGTAC	28855	0.0	18.460375	10-11
>>END_MODULE