FastQCFastQC Report
Fri 27 May 2016
SRR2138627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6621624
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104700.045.9110071
GTATCAA182200.035.64191
ATCAACG220300.028.9775033
TCAACGC222500.028.7098164
CAACGCA222500.028.6870825
TATCAAC227800.028.3211842
AACGCAG227650.028.2176786
ACGCAGA262300.024.3124077
CGCAGAG266550.023.9088618
GTGGTAT51950.022.4386961
TGGTATC53950.021.135442
GCAGAGT305600.020.8121559
GAGTACT164850.020.03627212-13
GTACATG225750.019.8846841
TACATGG224300.019.7108572
ACATGGG230300.018.7187773
CAGAGTA298700.018.5195910-11
AGAGTAC272500.017.95217710-11
GTACTTT185750.017.7591114-15
GTACTAG16350.017.3703081
Produced by FastQC (version 0.11.5)