##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138627_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6621624
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82697688663687	33.0	33.0	33.0	33.0	33.0
2	32.822184255705245	33.0	33.0	33.0	33.0	33.0
3	32.81866351819433	33.0	33.0	33.0	33.0	33.0
4	36.76968399292983	37.0	37.0	37.0	37.0	37.0
5	36.7820231109468	37.0	37.0	37.0	37.0	37.0
6	36.791661682994985	37.0	37.0	37.0	37.0	37.0
7	36.77733287181513	37.0	37.0	37.0	37.0	37.0
8	36.77595284782102	37.0	37.0	37.0	37.0	37.0
9	36.77475450131267	37.0	37.0	37.0	37.0	37.0
10-11	36.751960395214226	37.0	37.0	37.0	37.0	37.0
12-13	36.73637871917826	37.0	37.0	37.0	37.0	37.0
14-15	39.063868395426866	40.0	40.0	40.0	37.0	40.0
16-17	39.01400562762247	40.0	40.0	40.0	37.0	40.0
18-19	39.04155015446362	40.0	40.0	40.0	37.0	40.0
20-21	39.056690640831064	40.0	40.0	40.0	37.0	40.0
22-23	39.09104338228477	40.0	40.0	40.0	37.0	40.0
24-25	39.09224298825232	40.0	40.0	40.0	37.0	40.0
26-27	39.00948260433535	40.0	40.0	40.0	37.0	40.0
28-29	38.940211882150436	40.0	40.0	40.0	37.0	40.0
30-31	38.86996105447837	40.0	40.0	40.0	37.0	40.0
32-33	38.84297570278603	40.0	38.5	40.0	37.0	40.0
34-35	38.82272733170692	40.0	37.0	40.0	37.0	40.0
36-37	38.7913711260327	40.0	37.0	40.0	37.0	40.0
38-39	38.74513730273836	40.0	37.0	40.0	37.0	40.0
40-41	38.69967672220092	40.0	37.0	40.0	37.0	40.0
42-43	38.632229774182434	40.0	37.0	40.0	37.0	40.0
44-45	38.54489368862451	40.0	37.0	40.0	37.0	40.0
46-47	38.46264872895861	40.0	37.0	40.0	37.0	40.0
48-49	38.411800022270235	40.0	37.0	40.0	37.0	40.0
50-51	38.355478147567865	40.0	37.0	40.0	37.0	40.0
52-53	38.273050330345455	40.0	37.0	40.0	37.0	40.0
54-55	38.19411103837477	40.0	37.0	40.0	37.0	40.0
56-57	38.07806232531826	40.0	37.0	40.0	37.0	40.0
58-59	37.941023623502545	40.0	37.0	40.0	35.0	40.0
60-61	37.76134060714375	40.0	37.0	40.0	33.0	40.0
62-63	37.56254673054872	37.0	37.0	40.0	33.0	40.0
64-65	37.32655068451719	37.0	37.0	40.0	33.0	40.0
66-67	37.08442064972964	37.0	37.0	40.0	33.0	40.0
68-69	36.83607307742952	37.0	37.0	40.0	33.0	40.0
70-71	36.572861067235436	37.0	37.0	38.5	33.0	40.0
72-73	36.329992554086814	37.0	37.0	37.0	33.0	40.0
74-75	36.09471837943079	37.0	37.0	37.0	33.0	40.0
76-77	34.46626728168616	35.0	33.0	37.0	30.0	37.0
78-79	35.72044909113791	37.0	35.0	37.0	33.0	37.0
80-81	35.84931680055435	37.0	37.0	37.0	33.0	37.0
82-83	35.7901809053354	37.0	37.0	37.0	33.0	37.0
84-85	35.67393650286016	37.0	37.0	37.0	33.0	37.0
86-87	35.56755479957931	37.0	37.0	37.0	33.0	37.0
88-89	35.49715755740098	37.0	35.0	37.0	33.0	37.0
90-91	35.435310832146996	37.0	33.0	37.0	33.0	37.0
92-93	35.36355782022557	37.0	33.0	37.0	33.0	37.0
94-95	35.323316576773905	37.0	33.0	37.0	33.0	37.0
96-97	35.285460199653656	37.0	33.0	37.0	33.0	37.0
98-99	35.24126796396567	37.0	33.0	37.0	33.0	37.0
100	35.179863449654	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	17.0
21	85.0
22	310.0
23	1014.0
24	2494.0
25	5250.0
26	9776.0
27	16151.0
28	24570.0
29	36022.0
30	50173.0
31	69691.0
32	94351.0
33	130647.0
34	189673.0
35	298049.0
36	609314.0
37	1940611.0
38	3007192.0
39	136227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.9045992342664	20.061679732947688	14.538956002334173	23.494765030451745
2	14.801837132401358	20.39179512457971	41.68850420984339	23.117863533175548
3	18.34648390081181	25.83055778482081	30.053640300390516	25.769318013976868
4	12.732354458216562	17.670138267431824	38.45702940627849	31.14047786807313
5	13.686037891343187	37.52591231592513	35.0142529066202	13.77379688611148
6	30.39878132615201	37.26295845248839	18.478050097679965	13.860210123679629
7	27.010624583938924	32.57478829966788	22.58082005260341	17.83376706378979
8	25.00209918291948	36.19254128594435	20.613236269531463	18.192123261604706
9	25.95523394260985	16.9388053444291	21.173023415403836	35.93293729755722
10-11	24.78999864685763	26.6079741163195	28.052295026114436	20.54973221070843
12-13	25.51054695947701	24.08959644945107	28.437978356971037	21.96187823410088
14-15	22.660528595401974	25.35459579100233	26.347365842578803	25.637509771016898
16-17	21.947365178089242	28.602243195928974	27.024057844420042	22.426333781561745
18-19	22.0182994383251	27.716236983555696	28.89695941660233	21.36850416151687
20-21	22.943910579393116	26.741261364637754	28.81345936473486	21.50136869123427
22-23	22.777650362331016	26.611134341250352	28.62764300311353	21.983572293305105
24-25	22.41479599871745	26.75284221053224	28.756693635706842	22.075668155043466
26-27	22.337412793285978	26.977283889627678	28.583636437021376	22.101666880064965
28-29	22.226533293161683	27.151991530662382	28.58797916380726	22.033496012368673
30-31	22.49726835324924	26.91535372609345	28.562046906893997	22.025331013763317
32-33	22.339979537836932	27.092102813714448	28.472235930137106	22.09568171831151
34-35	22.41265694550288	27.09938881792037	28.49289979793771	21.995054438639038
36-37	22.452499270561262	27.006530293625296	28.373774661493762	22.167195774319687
38-39	22.31013982667225	27.154208688041386	28.454001259341783	22.081650225944582
40-41	22.547272342218015	27.07487473556579	28.274023270986206	22.103829651229994
42-43	22.412810680529873	27.154181868055865	28.10053407810203	22.332473373312233
44-45	22.56551979576076	27.293316194987185	27.857206516413267	22.283957492838784
46-47	22.61372729196476	27.24021069315981	27.53125751056386	22.61480450431156
48-49	22.628376342423543	27.41894190592745	27.428518410023024	22.524163341625982
50-51	22.515643456105586	27.458023799529474	27.403536088143515	22.622796656221425
52-53	22.683189208944516	27.43767454190962	27.16536927793431	22.71376697121156
54-55	22.690542963830676	27.315746332910244	27.23316728818582	22.760543415073254
56-57	22.815722532755274	27.354265321386084	27.200956668659725	22.62905547719891
58-59	22.606525762311044	27.554059207601494	27.27930504436607	22.56010998572139
60-61	22.77357012274745	27.415655642346685	27.227744180603935	22.58303005430193
62-63	22.748627310328693	27.53462489222614	27.04785732476581	22.668890472679358
64-65	22.68193143018316	27.489650526562748	27.110880715499132	22.71753732775496
66-67	22.81384930177754	27.511197447467705	26.917458965034996	22.757494285719755
68-69	22.710064065655875	27.41180339232323	27.079620541222273	22.798512000798624
70-71	22.682992541649106	27.192521227419597	27.16387586560402	22.960610365327277
72-73	22.796447874815957	27.247197903426596	27.06779709889001	22.888557122867432
74-75	22.688991784433544	27.481003372247688	27.017720364903713	22.81228447841506
76-77	22.846944232266218	27.38991415354019	26.84307796802079	22.920063646172803
78-79	22.779317864551427	27.415764055126463	26.81937536044244	22.98554271987967
80-81	22.730439159417195	27.410534050792005	26.943217179068746	22.91580961072205
82-83	22.833794666317083	27.333697566648503	27.094526245646012	22.73798152138841
84-85	22.97422388878359	27.374532157102248	26.899275679492778	22.751968274621387
86-87	22.675917873410366	27.41622249033427	27.0413957205409	22.866463915714462
88-89	22.815101419710448	27.334770938844276	26.960552178334314	22.88957546311096
90-91	22.828291982916372	27.455262262352104	26.946740856093736	22.769704898637784
92-93	22.90089695172299	27.3984121429929	26.977554071095927	22.72313683418818
94-95	22.70680779251391	27.459649492343146	27.031617907034477	22.801924808108467
96-97	22.82167704361996	27.551250033183106	27.12442337969439	22.502649543502542
98-99	23.978833787391267	29.430888397227626	27.206709942238703	19.383567873142407
100	22.951166598823225	30.40227306368649	21.020311401398807	25.62624893609148
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1435.0
1	1315.0
2	1871.5
3	2712.5
4	3909.5
5	6557.5
6	8564.5
7	9727.0
8	10320.0
9	9856.5
10	8860.0
11	8034.5
12	8916.0
13	12345.5
14	20077.0
15	30182.5
16	38281.0
17	43153.5
18	43903.5
19	40869.5
20	36284.5
21	31633.5
22	28570.5
23	29368.5
24	34460.5
25	42676.5
26	53200.0
27	65048.0
28	76297.0
29	89124.0
30	101308.0
31	113022.5
32	127717.5
33	141465.5
34	155222.0
35	168393.0
36	181941.5
37	196271.5
38	205677.5
39	211213.5
40	219538.5
41	228782.5
42	235861.5
43	243686.0
44	252905.0
45	263381.0
46	272464.0
47	279089.5
48	282707.5
49	282077.5
50	278906.0
51	275140.5
52	268943.5
53	257061.5
54	244546.0
55	230687.0
56	214629.5
57	196666.0
58	176680.0
59	156733.5
60	131945.5
61	106557.0
62	85390.5
63	66024.5
64	48605.0
65	35661.0
66	26733.0
67	20349.5
68	15319.0
69	11034.0
70	7819.0
71	5377.0
72	3580.5
73	2358.5
74	1553.5
75	989.5
76	677.5
77	456.5
78	307.0
79	200.0
80	111.0
81	65.0
82	46.0
83	35.0
84	25.0
85	19.0
86	15.0
87	11.0
88	7.5
89	6.0
90	5.5
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0016612238931114178
4	0.011462444862468784
5	0.018424483178144817
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0037916103747221343
26-27	0.042762144411237046
28-29	0.08917924029447216
30-31	0.0858948044127538
32-33	0.03565485553408439
34-35	0.0029315534057835664
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	8.264509255093334E-5
56-57	2.3333327500001458E-4
58-59	8.40560896199303E-6
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	8.871278809034367E-6
72-73	0.013510993194094595
74-75	0.027674901674137296
76-77	0.005599003049176937
78-79	0.0
80-81	0.0
82-83	0.0058031056944617895
84-85	0.046205607462456874
86-87	0.043196676642471996
88-89	0.010819099717358277
90-91	9.787341620014487E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	77.0
20-21	411.0
22-23	895.0
24-25	1653.0
26-27	3048.0
28-29	6566.0
30-31	16213.0
32-33	20125.0
34-35	27553.0
36-37	40351.0
38-39	47186.0
40-41	49854.0
42-43	57434.0
44-45	67159.0
46-47	65246.0
48-49	58411.0
50-51	50146.0
52-53	47182.0
54-55	49342.0
56-57	51612.0
58-59	51411.0
60-61	50668.0
62-63	49437.0
64-65	52179.0
66-67	54402.0
68-69	53474.0
70-71	52706.0
72-73	50460.0
74-75	50714.0
76-77	50715.0
78-79	53345.0
80-81	53905.0
82-83	51857.0
84-85	51235.0
86-87	53675.0
88-89	57272.0
90-91	60158.0
92-93	61625.0
94-95	74035.0
96-97	210343.0
98-99	393864.0
100-101	4323680.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.06145499304331
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.85916629401883	72.09851020562935
2	8.160266186829743	13.392866328435643
3	2.170454137328634	5.343318735145751
4	0.8166841889620027	2.6807317126414185
5	0.3630398574031697	1.4895789459485536
6	0.21159660878369324	1.0418355353030186
7	0.11895114409679658	0.6832912770369198
8	0.07556264465185612	0.4960624450602893
9	0.05451631418519463	0.40263192566054623
>10	0.16610221031871944	2.096903721813442
>50	0.003018190319572312	0.16392784408067657
>100	6.092110470778943E-4	0.08842537379479867
>500	3.3012054654808425E-5	0.021915949449623096
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.0204070783843963E-5	0.0	0.0	0.0
3	0.0	4.530610617576594E-5	0.0	0.0	0.0
4	0.0	9.061221235153189E-5	0.0	0.0	0.0
5	0.0	9.061221235153189E-5	0.0	0.0	0.0
6	0.0	1.0571424774345387E-4	0.0	0.0	3.0204070783843963E-5
7	0.0	1.2081628313537585E-4	0.0	0.0	3.0204070783843963E-5
8	0.0	1.2081628313537585E-4	0.0	1.5102035391921982E-5	3.0204070783843963E-5
9	0.0	2.2653053087882974E-4	0.0	4.530610617576594E-5	3.0204070783843963E-5
10-11	0.0	2.2653053087882974E-4	0.0	9.816323004749288E-5	3.0204070783843963E-5
12-13	0.0	2.2653053087882974E-4	0.0	1.585713716151808E-4	3.775508847980495E-5
14-15	0.0	3.020407078384396E-4	0.0	2.0387747779094677E-4	6.795915926364892E-5
16-17	0.0	3.171427432303616E-4	0.0	2.2653053087882974E-4	1.2081628313537585E-4
18-19	0.0	3.397957963182446E-4	0.0	2.416325662707517E-4	1.3591831852729784E-4
20-21	0.0	3.7755088479804954E-4	0.0	2.491835839667127E-4	1.3591831852729784E-4
22-23	0.0	4.983671679334254E-4	0.0	2.7183663705459567E-4	1.661223893111418E-4
24-25	0.0	5.436732741091913E-4	0.0	3.020407078384396E-4	2.0387747779094677E-4
26-27	0.0	5.587753095011133E-4	0.0	4.0775495558189353E-4	2.1142849548690774E-4
28-29	0.0	5.587753095011133E-4	0.0	9.891833181708898E-4	2.1897951318286874E-4
30-31	0.0	6.267344687647622E-4	0.0	0.0027561214590257617	2.2653053087882974E-4
32-33	0.0	6.418365041566843E-4	0.0	0.006086120262944559	2.567346016626737E-4
34-35	0.0	7.6265278729206E-4	0.0	0.010299588137290792	3.171427432303616E-4
36-37	0.0	8.75918052731475E-4	0.0	0.016868973532776853	3.171427432303616E-4
38-39	0.0	8.834690704274359E-4	0.0	0.03377570215403351	3.171427432303616E-4
40-41	0.0	0.0010873465482183827	0.0	0.051286512190967054	3.322447786222836E-4
42-43	0.0	0.0011402036720901095	0.0	0.06223548785011049	3.397957963182446E-4
44-45	0.0	0.0013591831852729783	0.0	0.07563099324274528	3.473468140142056E-4
46-47	0.0	0.0015102035391921982	0.0	0.0907254776169713	3.473468140142056E-4
48-49	0.0	0.0015177545568881591	0.0	0.10535179889404775	3.7755088479804954E-4
50-51	0.0	0.001729183052375067	0.0	0.11864914105663504	3.7755088479804954E-4
52-53	0.0	0.001736734070071028	0.0	0.15088443560069253	4.153059732778545E-4
54-55	0.0	0.001736734070071028	0.0	0.19061789071683927	4.5306106175765947E-4
56-57	1.5102035391921982E-5	0.001736734070071028	0.0	0.22828236698429266	4.5306106175765947E-4
58-59	1.5102035391921982E-5	0.001736734070071028	0.0	0.25181133812490714	4.6061207945362044E-4
60-61	1.5102035391921982E-5	0.00175183610546295	0.0	0.26907296457787394	4.681630971495814E-4
62-63	1.5102035391921982E-5	0.0017895911939427547	0.0	0.2821588782449743	5.436732741091913E-4
64-65	1.5102035391921982E-5	0.002167142078740804	0.0	0.28869805956967654	5.436732741091913E-4
66-67	1.5102035391921982E-5	0.0021897951318286873	0.0	0.29477662881492517	5.512242918051523E-4
68-69	1.5102035391921982E-5	0.0022124481849165704	0.0	0.30114968775031625	5.587753095011133E-4
70-71	1.5102035391921982E-5	0.0022426522557004143	0.0	0.3063900940313131	5.587753095011133E-4
72-73	1.5102035391921982E-5	0.0022577542910923363	0.0	0.3103694803570846	5.738773448930353E-4
74-75	1.5102035391921982E-5	0.0023181624326600245	0.0	0.31305009163915076	5.738773448930353E-4
76-77	1.5102035391921982E-5	0.002461631768883283	0.0	0.3159345803990079	5.738773448930353E-4
78-79	1.5102035391921982E-5	0.002506937875059049	0.0	0.31781478380530215	5.738773448930353E-4
80-81	1.5102035391921982E-5	0.002506937875059049	0.0	0.31847172234485077	6.040814156768792E-4
82-83	1.5102035391921982E-5	0.002506937875059049	0.0	0.31907580376052763	6.644895572445672E-4
84-85	1.5102035391921982E-5	0.002522039910450971	0.0	0.3193023342914064	6.644895572445672E-4
86-87	1.5102035391921982E-5	0.002522039910450971	0.0	0.31937029345067014	6.720405749405282E-4
88	1.5102035391921982E-5	0.002642856193586347	0.0	0.3193778444683661	6.795915926364892E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10470	0.0	45.911007	1
GTATCAA	18220	0.0	35.6419	1
ATCAACG	22030	0.0	28.977503	3
TCAACGC	22250	0.0	28.709816	4
CAACGCA	22250	0.0	28.687082	5
TATCAAC	22780	0.0	28.321184	2
AACGCAG	22765	0.0	28.217678	6
ACGCAGA	26230	0.0	24.312407	7
CGCAGAG	26655	0.0	23.908861	8
GTGGTAT	5195	0.0	22.438696	1
TGGTATC	5395	0.0	21.13544	2
GCAGAGT	30560	0.0	20.812155	9
GAGTACT	16485	0.0	20.036272	12-13
GTACATG	22575	0.0	19.884684	1
TACATGG	22430	0.0	19.710857	2
ACATGGG	23030	0.0	18.718777	3
CAGAGTA	29870	0.0	18.51959	10-11
AGAGTAC	27250	0.0	17.952177	10-11
GTACTTT	18575	0.0	17.75911	14-15
GTACTAG	1635	0.0	17.370308	1
>>END_MODULE