FastQCFastQC Report
Fri 27 May 2016
SRR2138626_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138626_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6918001
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101200.046.9184531
GTATCAA178600.037.3664251
ATCAACG218600.029.83213
TCAACGC220950.029.5912084
CAACGCA224000.029.129935
TATCAAC227700.028.937232
AACGCAG229350.028.4848546
ACGCAGA261250.024.974317
CGCAGAG263400.024.7543938
GTGGTAT52750.021.4200341
GCAGAGT310950.020.914569
TGGTATC51800.020.0155582
GAGTACT182550.019.43509512-13
AGAGTAC281450.018.61916410-11
CAGAGTA301100.018.42287410-11
GTACATG213500.018.215831
TACATGG210850.017.9430082
GTACTTT204100.017.2281714-15
AGTACTT198100.017.21535712-13
TATACCG6000.016.9263425
Produced by FastQC (version 0.11.5)