##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138626_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6918001
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.825571144034235	33.0	33.0	33.0	33.0	33.0
2	32.8217892712071	33.0	33.0	33.0	33.0	33.0
3	32.81844408522057	33.0	33.0	33.0	33.0	33.0
4	36.77011740819349	37.0	37.0	37.0	37.0	37.0
5	36.78405640588951	37.0	37.0	37.0	37.0	37.0
6	36.79184926397091	37.0	37.0	37.0	37.0	37.0
7	36.77829795052068	37.0	37.0	37.0	37.0	37.0
8	36.776632006847066	37.0	37.0	37.0	37.0	37.0
9	36.77531486335431	37.0	37.0	37.0	37.0	37.0
10-11	36.75360302781107	37.0	37.0	37.0	37.0	37.0
12-13	36.73866150062713	37.0	37.0	37.0	37.0	37.0
14-15	39.06600179734001	40.0	40.0	40.0	37.0	40.0
16-17	39.01810421825611	40.0	40.0	40.0	37.0	40.0
18-19	39.04846739686796	40.0	40.0	40.0	37.0	40.0
20-21	39.06443162666099	40.0	40.0	40.0	37.0	40.0
22-23	39.09891282810224	40.0	40.0	40.0	37.0	40.0
24-25	39.10059255733103	40.0	40.0	40.0	37.0	40.0
26-27	39.01930546316586	40.0	40.0	40.0	37.0	40.0
28-29	38.9506071080386	40.0	40.0	40.0	37.0	40.0
30-31	38.87963957611052	40.0	40.0	40.0	37.0	40.0
32-33	38.85320855923455	40.0	40.0	40.0	37.0	40.0
34-35	38.833309124702666	40.0	37.0	40.0	37.0	40.0
36-37	38.801633625792604	40.0	37.0	40.0	37.0	40.0
38-39	38.75824375465196	40.0	37.0	40.0	37.0	40.0
40-41	38.718352984571204	40.0	37.0	40.0	37.0	40.0
42-43	38.653989555754215	40.0	37.0	40.0	37.0	40.0
44-45	38.57080809991635	40.0	37.0	40.0	37.0	40.0
46-47	38.4920389702147	40.0	37.0	40.0	37.0	40.0
48-49	38.45095874523442	40.0	37.0	40.0	37.0	40.0
50-51	38.4043194966319	40.0	37.0	40.0	37.0	40.0
52-53	38.332046604083644	40.0	37.0	40.0	37.0	40.0
54-55	38.26666662176221	40.0	37.0	40.0	37.0	40.0
56-57	38.16344559132568	40.0	37.0	40.0	37.0	40.0
58-59	38.040944407232274	40.0	37.0	40.0	37.0	40.0
60-61	37.87221102696501	40.0	37.0	40.0	35.0	40.0
62-63	37.68395534521614	38.5	37.0	40.0	33.0	40.0
64-65	37.45821935666455	37.0	37.0	40.0	33.0	40.0
66-67	37.22225292425971	37.0	37.0	40.0	33.0	40.0
68-69	36.96858403997103	37.0	37.0	40.0	33.0	40.0
70-71	36.701305568530834	37.0	37.0	40.0	33.0	40.0
72-73	36.450105011822714	37.0	37.0	37.0	33.0	40.0
74-75	36.20656027013327	37.0	37.0	37.0	33.0	40.0
76-77	34.590752180966014	35.0	33.0	37.0	30.0	37.0
78-79	35.80885181132513	37.0	37.0	37.0	33.0	37.0
80-81	35.91884501086811	37.0	37.0	37.0	33.0	37.0
82-83	35.84820039510079	37.0	37.0	37.0	33.0	37.0
84-85	35.72582748720227	37.0	37.0	37.0	33.0	37.0
86-87	35.61307182950756	37.0	37.0	37.0	33.0	37.0
88-89	35.54045672879764	37.0	37.0	37.0	33.0	37.0
90-91	35.47698792301425	37.0	33.0	37.0	33.0	37.0
92-93	35.40520394458422	37.0	33.0	37.0	33.0	37.0
94-95	35.358993820451055	37.0	33.0	37.0	33.0	37.0
96-97	35.324203495280315	37.0	33.0	37.0	33.0	37.0
98-99	35.28162483779538	37.0	33.0	37.0	33.0	37.0
100	35.21855833291545	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	26.0
21	93.0
22	349.0
23	1048.0
24	2516.0
25	5295.0
26	10041.0
27	16410.0
28	25422.0
29	36369.0
30	51382.0
31	70812.0
32	96461.0
33	133303.0
34	192811.0
35	301037.0
36	603838.0
37	1934192.0
38	3288007.0
39	148585.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.65619519280208	20.319222272445465	14.701573474765325	23.323009059987125
2	14.52507740314001	20.85773043397941	41.96911795762967	22.64807420525091
3	18.17291110439744	26.010214097072954	30.394137518809927	25.42273727971968
4	12.46514333644102	18.057952740515496	39.10879702749225	30.36810689555123
5	13.366798194626586	37.69972619264651	35.40725645147463	13.526219161252277
6	29.831305314931296	37.82315151443314	18.81748788414457	13.528055286490998
7	26.468455266196116	32.915534415216186	22.908626350299745	17.70738396828795
8	24.471057462986778	36.521633922862975	21.06792121018774	17.939387403962503
9	25.55732501339621	17.137508364049094	21.426970016338533	35.878196606216164
10-11	24.214574412463946	26.905199926973122	28.599005695431384	20.281219965131548
12-13	25.11904811809076	24.433221388664155	28.880907938579366	21.56682255466572
14-15	22.20130497234678	25.80775140101888	26.84772667711381	25.143216949520536
16-17	21.522662399152587	28.99474862752983	27.445789614658917	22.036799358658666
18-19	21.478364920733604	28.050610284676164	29.470044887244164	21.000979907346068
20-21	22.4270484311367	27.124408576488364	29.380429709087068	21.068113283287868
22-23	22.141529576831758	27.045899130322148	29.217733746026198	21.594837546819896
24-25	21.850041174042207	27.174108721161	29.339906720370053	21.63594338442674
26-27	21.80221249172763	27.41459009373075	29.171534345743055	21.611663068798563
28-29	21.636130749951203	27.562813406132342	29.207439868211445	21.593615975705017
30-31	22.040562465138926	27.30373562795338	29.064864103173484	21.590837803734214
32-33	21.808147715313993	27.519731907693917	28.95255911577797	21.719561261214118
34-35	21.887516356783827	27.593688377297692	29.028434377859018	21.490360888059456
36-37	21.922467954021112	27.489773097572023	28.920847279941693	21.66691166846518
38-39	21.760495870462083	27.617833716722746	28.977448562074027	21.644221850741143
40-41	22.061463622515056	27.44201575885165	28.811071196558895	21.6854494220744
42-43	21.942789916530305	27.575451366066524	28.576394152388062	21.90536456501511
44-45	22.08857391692085	27.731872095728242	28.351477799951656	21.828076187399244
46-47	22.13532229124673	27.678813084257914	28.035136441913444	22.150728182581908
48-49	22.15219132793576	27.844684200936015	27.967755148985518	22.035369322142706
50-51	22.115263863286764	27.94652553235425	27.857612412227915	22.08059819213107
52-53	22.237477034609075	27.890283547231647	27.70355049776881	22.16868892039047
54-55	22.233032267284734	27.77202594653757	27.76635377567341	22.22858801050429
56-57	22.258802698737885	27.882238635673584	27.679809324132993	22.179149341455542
58-59	22.169935565572814	27.97785965249384	27.764263040508	22.087941741425347
60-61	22.305526382294865	27.906122958761674	27.722908737981548	22.065441920961913
62-63	22.248648285148892	27.929377050739845	27.62835447937758	22.19362018473368
64-65	22.199646141090756	27.95908081388707	27.632957865245917	22.208315179776257
66-67	22.291908549989	27.942661923666552	27.48095904504214	22.284470481302314
68-69	22.267826841437838	27.92826318958061	27.55701602698288	22.246893941998678
70-71	22.22297563510934	27.672825093855913	27.61827308521131	22.485926185823438
72-73	22.295104407946194	27.737099592632276	27.592018423092895	22.375777576328634
74-75	22.173497371961112	27.90229277323977	27.544681861677784	22.379527993121336
76-77	22.280421677710052	27.817366761447555	27.43054282970939	22.471668731133008
78-79	22.27898460015463	27.853289726121456	27.40855444918519	22.459171224538725
80-81	22.24141191414055	27.869016526802536	27.451368670434434	22.43820288862248
82-83	22.350652949140475	27.79271427251982	27.588609377147115	22.268023401192586
84-85	22.48960537145861	27.79944864838193	27.440217059308125	22.270728920851337
86-87	22.19757156661971	27.8760692912795	27.56283932518363	22.363519816917158
88-89	22.317807413336705	27.79037392774402	27.490983494692568	22.4008351642267
90-91	22.37645050174822	27.76772354211338	27.522604178782956	22.33322177735544
92-93	22.38014847059768	27.76977112161495	27.545034350868512	22.305046056918865
94-95	22.200198349653853	27.92503153374351	27.553963623058632	22.32080649354401
96-97	22.287982727250007	27.986088889389816	27.617200420841968	22.10872796251821
98-99	23.36012564475206	29.904494212869213	27.83057974781994	18.904800394558787
100	22.303530768134998	30.919993130438677	21.654761054202528	25.121715047223798
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1263.0
1	1238.0
2	1860.0
3	2729.0
4	3924.5
5	6502.5
6	8485.0
7	9711.0
8	10433.5
9	10181.5
10	9486.0
11	8901.5
12	9968.0
13	13446.5
14	21176.5
15	31609.0
16	40405.0
17	45925.0
18	46880.5
19	44053.5
20	39673.0
21	35039.5
22	32192.0
23	33112.5
24	38076.5
25	46610.5
26	57999.0
27	70641.5
28	83999.5
29	99857.5
30	115309.0
31	129657.0
32	145923.5
33	161411.5
34	176889.0
35	192961.0
36	210107.5
37	226526.0
38	237974.0
39	246268.5
40	255841.0
41	265318.0
42	272372.5
43	279497.5
44	285565.0
45	291773.0
46	297430.0
47	299122.5
48	296852.0
49	291376.0
50	284057.0
51	274782.5
52	261813.5
53	246662.5
54	231875.0
55	215082.0
56	196011.0
57	175411.5
58	154776.0
59	135005.0
60	113269.0
61	91740.0
62	72471.5
63	55527.0
64	40850.5
65	29462.5
66	21920.5
67	16568.0
68	12414.5
69	8569.0
70	5697.0
71	3964.5
72	2645.0
73	1660.5
74	1053.5
75	683.5
76	468.5
77	302.0
78	187.5
79	122.5
80	79.5
81	51.5
82	37.0
83	25.5
84	17.5
85	15.0
86	13.0
87	12.0
88	8.5
89	3.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0016045097420483172
4	0.010754551784540071
5	0.015813816736944673
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.004120849328735333
26-27	0.04322518142715852
28-29	0.08948334763817438
30-31	0.08543924292323801
32-33	0.036093088783767525
34-35	0.0030398090329274013
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	6.337617060738375E-5
56-57	1.8377207851733965E-4
58-59	1.611988812152848E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.4031185327454446E-5
72-73	0.013575363488729314
74-75	0.027321710673568988
76-77	0.005116803951537132
78-79	0.0
80-81	0.0
82-83	0.006226113708478759
84-85	0.04685412197977911
86-87	0.04360365855283832
88-89	0.011658014964246587
90-91	1.503278744897543E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	89.0
20-21	382.0
22-23	958.0
24-25	2125.0
26-27	3392.0
28-29	6887.0
30-31	17079.0
32-33	21557.0
34-35	29202.0
36-37	42703.0
38-39	50910.0
40-41	52497.0
42-43	61523.0
44-45	71224.0
46-47	68541.0
48-49	61212.0
50-51	53063.0
52-53	49988.0
54-55	53343.0
56-57	54359.0
58-59	54160.0
60-61	52928.0
62-63	52084.0
64-65	54226.0
66-67	56279.0
68-69	56355.0
70-71	54874.0
72-73	52670.0
74-75	53663.0
76-77	53809.0
78-79	56047.0
80-81	56805.0
82-83	54802.0
84-85	54481.0
86-87	56423.0
88-89	60103.0
90-91	62554.0
92-93	64037.0
94-95	76816.0
96-97	216211.0
98-99	410802.0
100-101	4506838.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.1294493806191
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.68661280771846	76.34971965133853
2	7.289001804355073	12.410174202781729
3	1.6767825158986334	4.282307169284996
4	0.630075930725188	2.145520682024654
5	0.2898012939509595	1.2335312291918061
6	0.14402326832317452	0.7356372918209404
7	0.08496573518061847	0.5063160376501883
8	0.04902658431662816	0.3338884902309641
9	0.0372728331309915	0.2855714185167303
>10	0.1094498087149673	1.4214748705121703
>50	0.0023223534811465923	0.13018319574655027
>100	5.842471987053558E-4	0.10488035201485857
>500	5.3878044768681006E-5	0.02944601300863682
>1k	2.69389607287591E-5	0.031349395877225995
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.445504272115601E-5
2	0.0	5.782017088462404E-5	0.0	0.0	1.445504272115601E-5
3	0.0	5.782017088462404E-5	0.0	0.0	1.445504272115601E-5
4	0.0	8.673025632693607E-5	0.0	0.0	2.891008544231202E-5
5	0.0	8.673025632693607E-5	0.0	0.0	2.891008544231202E-5
6	0.0	8.673025632693607E-5	0.0	0.0	4.3365128163468034E-5
7	0.0	8.673025632693607E-5	0.0	0.0	4.3365128163468034E-5
8	0.0	1.1564034176924809E-4	0.0	0.0	4.3365128163468034E-5
9	0.0	3.035558971442762E-4	0.0	1.445504272115601E-5	4.3365128163468034E-5
10-11	0.0	3.035558971442762E-4	0.0	1.445504272115601E-5	4.3365128163468034E-5
12-13	0.0	3.035558971442762E-4	0.0	1.445504272115601E-5	4.3365128163468034E-5
14-15	0.0	3.9751367483179033E-4	0.0	2.891008544231202E-5	5.782017088462404E-5
16-17	0.0	4.047411961923683E-4	0.0	2.891008544231202E-5	5.782017088462404E-5
18-19	0.0	4.191962389135243E-4	0.0	2.891008544231202E-5	5.782017088462404E-5
20-21	0.0	4.336512816346803E-4	0.0	2.891008544231202E-5	5.782017088462404E-5
22-23	0.0	4.6256136707699234E-4	0.0	2.891008544231202E-5	5.782017088462404E-5
24-25	0.0	4.986989738798823E-4	0.0	5.782017088462404E-5	5.782017088462404E-5
26-27	0.0	5.203815379616163E-4	0.0	8.673025632693607E-5	5.782017088462404E-5
28-29	0.0	5.203815379616163E-4	0.0	4.4810632435583633E-4	5.782017088462404E-5
30-31	0.0	6.071117942885524E-4	0.0	0.0016984675197358311	5.782017088462404E-5
32-33	0.0	6.071117942885524E-4	0.0	0.004531655893082409	5.782017088462404E-5
34-35	0.0	7.082970933366445E-4	0.0	0.007437119480034767	5.782017088462404E-5
36-37	1.445504272115601E-5	8.094823923847366E-4	0.0	0.012200056056655672	5.782017088462404E-5
38-39	1.445504272115601E-5	8.239374351058926E-4	0.0	0.02294015279847459	6.504769224520204E-5
40-41	2.1682564081734017E-5	8.383924778270486E-4	0.0	0.035450992273635115	8.673025632693607E-5
42-43	2.891008544231202E-5	8.528475205482047E-4	0.0	0.04374818679557867	8.673025632693607E-5
44-45	2.891008544231202E-5	9.901704263991866E-4	0.0	0.053519795675080126	8.673025632693607E-5
46-47	2.891008544231202E-5	0.0010985832468078567	0.0	0.06309626147784599	8.673025632693607E-5
48-49	2.891008544231202E-5	0.0010985832468078567	0.0	0.07312806112632825	8.673025632693607E-5
50-51	2.891008544231202E-5	0.0012214511099376828	0.0	0.08295026265535377	8.673025632693607E-5
52-53	2.891008544231202E-5	0.0012286786312982608	0.0	0.1016984530646931	1.0118529904809208E-4
54-55	2.891008544231202E-5	0.0012359061526588389	0.0	0.12476147372629753	1.1564034176924809E-4
56-57	2.891008544231202E-5	0.0012864988021828849	0.0	0.14942900412995025	1.1564034176924809E-4
58-59	2.891008544231202E-5	0.0012864988021828849	0.0	0.1707718747077371	1.1564034176924809E-4
60-61	2.891008544231202E-5	0.0012937263235434629	0.0	0.1809626798261521	1.1564034176924809E-4
62-63	2.891008544231202E-5	0.001322636408985775	0.0	0.1925483965671586	1.2286786312982608E-4
64-65	2.891008544231202E-5	0.001488869400279069	0.0	0.19921217126161156	1.3009538449040408E-4
66-67	2.891008544231202E-5	0.0014960969216396471	0.0	0.20477736270925662	1.3009538449040408E-4
68-69	2.891008544231202E-5	0.001532234528442537	0.0	0.21077620543853637	1.3009538449040408E-4
70-71	2.891008544231202E-5	0.0015539170925242712	0.0	0.21603061346767657	1.445504272115601E-4
72-73	2.891008544231202E-5	0.001568372135245427	0.0	0.21988288235286463	1.445504272115601E-4
74-75	2.891008544231202E-5	0.0016478748702117851	0.0	0.2225570652562785	1.445504272115601E-4
76-77	2.891008544231202E-5	0.0017201500838175652	0.0	0.22544084627914912	1.445504272115601E-4
78-79	2.891008544231202E-5	0.0017346051265387213	0.0	0.22757296508051963	1.445504272115601E-4
80-81	2.891008544231202E-5	0.0017346051265387213	0.0	0.22833908234474093	1.445504272115601E-4
82-83	3.613760680289003E-5	0.0017346051265387213	0.0	0.2287799611477362	1.445504272115601E-4
84-85	4.3365128163468034E-5	0.0017346051265387213	0.0	0.22897510422447176	1.445504272115601E-4
86-87	4.3365128163468034E-5	0.0017707427333416112	0.0	0.2290690620021593	1.445504272115601E-4
88	4.3365128163468034E-5	0.0018791555537502813	0.0	0.22908351704488045	1.445504272115601E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10120	0.0	46.918453	1
GTATCAA	17860	0.0	37.366425	1
ATCAACG	21860	0.0	29.8321	3
TCAACGC	22095	0.0	29.591208	4
CAACGCA	22400	0.0	29.12993	5
TATCAAC	22770	0.0	28.93723	2
AACGCAG	22935	0.0	28.484854	6
ACGCAGA	26125	0.0	24.97431	7
CGCAGAG	26340	0.0	24.754393	8
GTGGTAT	5275	0.0	21.420034	1
GCAGAGT	31095	0.0	20.91456	9
TGGTATC	5180	0.0	20.015558	2
GAGTACT	18255	0.0	19.435095	12-13
AGAGTAC	28145	0.0	18.619164	10-11
CAGAGTA	30110	0.0	18.422874	10-11
GTACATG	21350	0.0	18.21583	1
TACATGG	21085	0.0	17.943008	2
GTACTTT	20410	0.0	17.22817	14-15
AGTACTT	19810	0.0	17.215357	12-13
TATACCG	600	0.0	16.926342	5
>>END_MODULE