FastQCFastQC Report
Fri 27 May 2016
SRR2138625_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138625_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7603916
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117700.049.1746751
CTTATAC80850.048.5304261
TATACAC112250.037.602973
GTATCAA213250.037.017961
TTATACA109000.036.8152242
ATCAACG255500.030.1985533
TCAACGC258200.029.9810494
CAACGCA260400.029.6271825
TATCAAC262850.029.5318282
AACGCAG267450.028.8580996
TACACAT144300.028.7183785
ACACATC145150.027.2300876
ACATCTC155750.025.7584788
ACGCAGA304300.025.34957
CGCAGAG311400.024.7578958
ATACACA182250.024.3947034
CACATCT163800.023.9223737
GTGGTAT53850.023.1837521
GCAGAGT355500.021.5791829
CATCTCC184900.021.3072559
Produced by FastQC (version 0.11.5)