##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138625_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7603916
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8238160442593	33.0	33.0	33.0	33.0	33.0
2	32.82457262810373	33.0	33.0	33.0	33.0	33.0
3	32.82012176357551	33.0	33.0	33.0	33.0	33.0
4	36.77090738508947	37.0	37.0	37.0	37.0	37.0
5	36.7840347000151	37.0	37.0	37.0	37.0	37.0
6	36.791826474674366	37.0	37.0	37.0	37.0	37.0
7	36.77596714640193	37.0	37.0	37.0	37.0	37.0
8	36.77525396124839	37.0	37.0	37.0	37.0	37.0
9	36.77568308224341	37.0	37.0	37.0	37.0	37.0
10-11	36.75304224823104	37.0	37.0	37.0	37.0	37.0
12-13	36.73581000894802	37.0	37.0	37.0	37.0	37.0
14-15	39.05434259137003	40.0	40.0	40.0	37.0	40.0
16-17	39.0017537674009	40.0	40.0	40.0	37.0	40.0
18-19	39.03288969788724	40.0	40.0	40.0	37.0	40.0
20-21	39.04881497880834	40.0	40.0	40.0	37.0	40.0
22-23	39.0810677312797	40.0	40.0	40.0	37.0	40.0
24-25	39.07905567302302	40.0	40.0	40.0	37.0	40.0
26-27	38.98865005188989	40.0	40.0	40.0	37.0	40.0
28-29	38.913093088579494	40.0	40.0	40.0	37.0	40.0
30-31	38.843942152064955	40.0	38.5	40.0	37.0	40.0
32-33	38.81651862233879	40.0	37.0	40.0	37.0	40.0
34-35	38.788910537797676	40.0	37.0	40.0	37.0	40.0
36-37	38.7533271454204	40.0	37.0	40.0	37.0	40.0
38-39	38.69381592824496	40.0	37.0	40.0	37.0	40.0
40-41	38.66655024179649	40.0	37.0	40.0	37.0	40.0
42-43	38.60523675490454	40.0	37.0	40.0	37.0	40.0
44-45	38.51839758216116	40.0	37.0	40.0	37.0	40.0
46-47	38.431036710748316	40.0	37.0	40.0	37.0	40.0
48-49	38.391420304330296	40.0	37.0	40.0	37.0	40.0
50-51	38.33029723691851	40.0	37.0	40.0	37.0	40.0
52-53	38.268370475671276	40.0	37.0	40.0	37.0	40.0
54-55	38.181930276286906	40.0	37.0	40.0	37.0	40.0
56-57	38.05857133031719	40.0	37.0	40.0	37.0	40.0
58-59	37.93896828104698	40.0	37.0	40.0	35.0	40.0
60-61	37.758357260196426	40.0	37.0	40.0	33.0	40.0
62-63	37.567133665069406	37.0	37.0	40.0	33.0	40.0
64-65	37.344842726038294	37.0	37.0	40.0	33.0	40.0
66-67	37.104824522855225	37.0	37.0	40.0	33.0	40.0
68-69	36.86514335300697	37.0	37.0	40.0	33.0	40.0
70-71	36.60340484954601	37.0	37.0	40.0	33.0	40.0
72-73	36.35668963557971	37.0	37.0	37.0	33.0	40.0
74-75	36.12030564474019	37.0	37.0	37.0	33.0	40.0
76-77	34.50492900187311	35.0	33.0	37.0	30.0	37.0
78-79	35.74611944692399	37.0	35.0	37.0	33.0	37.0
80-81	35.863854519655995	37.0	37.0	37.0	33.0	37.0
82-83	35.80112421410031	37.0	37.0	37.0	33.0	37.0
84-85	35.681611478157365	37.0	37.0	37.0	33.0	37.0
86-87	35.57376543182089	37.0	37.0	37.0	33.0	37.0
88-89	35.50340451903848	37.0	35.0	37.0	33.0	37.0
90-91	35.43997369937978	37.0	33.0	37.0	33.0	37.0
92-93	35.36812067899347	37.0	33.0	37.0	33.0	37.0
94-95	35.32188091581839	37.0	33.0	37.0	33.0	37.0
96-97	35.28716755373725	37.0	33.0	37.0	33.0	37.0
98-99	35.241224016260475	37.0	33.0	37.0	33.0	37.0
100	35.17529383947251	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	10.0
20	22.0
21	108.0
22	450.0
23	1280.0
24	3219.0
25	6632.0
26	12130.0
27	19911.0
28	29703.0
29	42998.0
30	58932.0
31	81349.0
32	110009.0
33	152352.0
34	222226.0
35	348278.0
36	693259.0
37	2189797.0
38	3485665.0
39	145585.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.365817297297866	20.189768009010095	14.617599668381398	23.826815025310644
2	14.557209732458906	20.57396478340897	41.88272200797589	22.986103476156234
3	18.054614685671428	25.888627220754312	30.750578100704928	25.30617999286934
4	12.560739683575594	18.233091236084867	37.968390177588404	31.23777890275114
5	13.513390154798548	37.410999975534445	35.24911325525227	13.826496614414735
6	29.9747130294443	37.38770917511451	18.481161548865085	14.156416246576105
7	26.50769682358406	32.8438925416851	22.275364430643368	18.373046204087473
8	24.74628862286222	36.2504004515568	20.914249973303228	18.089060952277748
9	25.416706339207323	16.906170452172276	20.90802949427637	36.76909371434403
10-11	24.372277389702884	26.58974665159373	28.288831701980925	20.749144256722456
12-13	25.287042886849353	24.153035094022606	28.4734208005454	22.086501218582637
14-15	22.481087376557028	25.529483755475468	26.15362926155418	25.835799606413328
16-17	21.895014358391123	28.92533268384343	26.764801189281943	22.4148517684835
18-19	21.94845392821278	27.64075116032318	28.89217871423093	21.518616197233108
20-21	22.788838705995023	26.664634333989763	28.75607825725807	21.790448702757143
22-23	22.534404344686397	26.917390313062818	28.56255544937976	21.985649892871027
24-25	22.335664613503514	27.205402837504018	28.79003178085537	21.668900768137103
26-27	22.385251222174386	27.248386124156852	28.779866413344447	21.586496240324315
28-29	22.02762787873332	27.002982984211332	28.983965776116634	21.985423360938718
30-31	22.45699183484569	27.055339695255377	28.677140220455076	21.810528249443863
32-33	22.303991155840176	26.884032712656097	28.832693459551052	21.979282671952678
34-35	21.983121098812067	27.27750900865552	29.16829271396485	21.571077178567567
36-37	22.408866076352805	27.095816334531364	28.54816974702907	21.94714784208676
38-39	22.143129732661137	26.928254363588888	28.793674386218626	22.134941517531352
40-41	22.21710302574616	26.79198199037324	28.833013713051304	22.157901270829292
42-43	22.425248594428894	26.86437252665469	28.65011893098202	22.060259947934398
44-45	22.196894304408442	27.171530106063734	28.4246185718258	22.206957017702024
46-47	22.642033080954654	26.93137845099035	27.797232730800065	22.629355737254933
48-49	22.433765214459232	27.120900534437375	27.838057203172	22.607277047931397
50-51	22.296055223190635	27.325939938606208	27.88452302379681	22.493481814406348
52-53	22.411899707445215	27.406414526643243	27.959211650049358	22.222474115862177
54-55	22.370300546869334	27.369641014571954	27.686923159062633	22.573135279496086
56-57	22.666077772482307	27.68269010396397	27.274083560426977	22.37714856312675
58-59	22.20475846104087	28.03831044470206	27.74419001864282	22.01274107561425
60-61	22.647326384611553	28.258012144549827	27.083169322065647	22.011492148772973
62-63	22.382493744988118	28.45858358010767	27.01453977743084	22.144382897473363
64-65	22.378551343763633	28.216584668984908	27.148637319873153	22.25622666737831
66-67	22.559438820479862	27.935647420935528	27.09894092040517	22.405972838179448
68-69	22.447561585780416	27.70803249360175	27.320958422149033	22.5234474984688
70-71	22.51142997906527	27.506670258364075	27.316853586475837	22.665046176094823
72-73	22.637478471342902	27.493994124677013	27.246576393704665	22.62195101027542
74-75	22.46173111455907	27.646969256536963	27.259435642081716	22.631863986822253
76-77	22.605337396113974	27.578883586582993	27.160920533882372	22.654858483420668
78-79	22.54517848297968	27.571329467522865	27.160101405319896	22.723390644177556
80-81	22.566531388733228	27.589390829415766	27.216388709002793	22.627689072848217
82-83	22.571989391710495	27.580696916598075	27.320771165496115	22.526542526195318
84-85	22.737212884370443	27.58081529484171	27.138281206288816	22.54369061449903
86-87	22.532300681464534	27.62158563946904	27.252826480903597	22.59328719816283
88-89	22.595832425745936	27.54956600973269	27.196214777853626	22.658386786667748
90-91	22.652136976311695	27.608860003686896	27.164009594061927	22.57499342593948
92-93	22.6889451399889	27.54982130844733	27.22750330480447	22.533730246759305
94-95	22.513797417721452	27.668352331552327	27.23786856494288	22.57998168578334
96-97	22.59245948091698	27.76208359771154	27.279766046890085	22.3656908744814
98-99	23.779731877241744	29.679819246170467	27.361409713544166	19.17903916304362
100	22.61841124656177	30.7069407073298	21.22733662365701	25.447311422451417
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2470.0
1	2117.5
2	2433.0
3	3317.0
4	4428.0
5	6911.0
6	9079.5
7	10827.0
8	12493.5
9	13053.5
10	12349.0
11	11318.0
12	11928.0
13	15093.0
14	22950.0
15	33747.5
16	42562.0
17	47850.5
18	48749.0
19	45652.0
20	40857.0
21	35775.0
22	32643.0
23	33442.5
24	38143.5
25	46496.0
26	57354.0
27	69665.0
28	83196.0
29	98432.5
30	113901.5
31	129644.0
32	147171.0
33	164428.0
34	180017.0
35	194518.0
36	211402.5
37	229581.5
38	242510.0
39	250637.5
40	259744.0
41	269162.5
42	276161.5
43	284358.5
44	295366.5
45	309954.0
46	329481.0
47	343894.0
48	340682.5
49	333548.0
50	327468.0
51	313794.0
52	298060.5
53	281863.5
54	267115.0
55	251349.0
56	233140.0
57	211831.5
58	188742.0
59	164122.0
60	136485.0
61	111125.0
62	88531.0
63	68672.5
64	52173.0
65	39560.0
66	30271.0
67	23062.0
68	17270.0
69	11991.0
70	8033.5
71	5645.0
72	3818.0
73	2423.0
74	1489.5
75	971.0
76	642.5
77	387.0
78	237.0
79	162.0
80	119.0
81	85.0
82	55.5
83	34.5
84	23.5
85	17.5
86	15.0
87	13.0
88	9.5
89	5.0
90	3.0
91	4.0
92	3.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0018017032276526988
4	0.010165814561865228
5	0.018280054645527382
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.003920322729386568
26-27	0.04243715481151725
28-29	0.0901140727221752
30-31	0.08673217860077975
32-33	0.035650287006286474
34-35	0.002929450481483686
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	9.992903610878617E-5
56-57	2.4481194878159615E-4
58-59	2.1804460073235365E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.319551460602225E-5
72-73	0.011930779023725406
74-75	0.025019755542363353
76-77	0.005384850678249304
78-79	0.0
80-81	0.0
82-83	0.005721283050698962
84-85	0.04290699364257677
86-87	0.040143300626006385
88-89	0.010705450633662917
90-91	1.2707575061739751E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	104.0
20-21	412.0
22-23	937.0
24-25	3301.0
26-27	3966.0
28-29	7039.0
30-31	16907.0
32-33	20756.0
34-35	28815.0
36-37	41217.0
38-39	49320.0
40-41	50737.0
42-43	59048.0
44-45	70325.0
46-47	67661.0
48-49	59373.0
50-51	53606.0
52-53	51577.0
54-55	58325.0
56-57	64831.0
58-59	64011.0
60-61	66291.0
62-63	67958.0
64-65	73197.0
66-67	79853.0
68-69	63380.0
70-71	55812.0
72-73	52858.0
74-75	54134.0
76-77	54466.0
78-79	56953.0
80-81	57796.0
82-83	55821.0
84-85	55410.0
86-87	58232.0
88-89	61576.0
90-91	63921.0
92-93	66628.0
94-95	81187.0
96-97	241440.0
98-99	453070.0
100-101	5011665.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.14757312963475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.41593420249299	76.13549796461382
2	7.4990334611481995	12.770490000693888
3	1.7620433630550614	4.5010114833995525
4	0.6358659581329	2.165697726830655
5	0.2837727859526189	1.2081282022050404
6	0.14476746497899207	0.739595898665434
7	0.07803222056037448	0.4650977944642593
8	0.04547026418755079	0.30973461161066346
9	0.032388329591874196	0.24820088962237394
>10	0.10039676462455112	1.2975631628210624
>50	0.0018760109126600521	0.10079090720250031
>100	4.1917436217375455E-4	0.058191357870851675
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.31511184500197E-5
2	0.0	0.0	0.0	0.0	1.31511184500197E-5
3	0.0	0.0	0.0	0.0	1.31511184500197E-5
4	0.0	0.0	0.0	0.0	1.31511184500197E-5
5	0.0	0.0	0.0	0.0	1.31511184500197E-5
6	6.57555922500985E-5	0.0	0.0	0.0	1.31511184500197E-5
7	6.57555922500985E-5	0.0	0.0	0.0	1.31511184500197E-5
8	6.57555922500985E-5	0.0	0.0	0.0	2.63022369000394E-5
9	6.57555922500985E-5	2.63022369000394E-5	0.0	0.0	2.63022369000394E-5
10-11	6.57555922500985E-5	2.63022369000394E-5	0.0	0.0	2.63022369000394E-5
12-13	6.57555922500985E-5	2.63022369000394E-5	0.0	0.0	2.63022369000394E-5
14-15	7.89067107001182E-5	6.57555922500985E-5	0.0	0.0	3.287779612504925E-5
16-17	1.4466230295021672E-4	6.57555922500985E-5	0.0	0.0	3.94533553500591E-5
18-19	1.4466230295021672E-4	6.57555922500985E-5	0.0	0.0	3.94533553500591E-5
20-21	1.4466230295021672E-4	6.57555922500985E-5	0.0	6.57555922500985E-6	3.94533553500591E-5
22-23	1.4466230295021672E-4	6.57555922500985E-5	0.0	1.31511184500197E-5	5.26044738000788E-5
24-25	1.578134214002364E-4	7.89067107001182E-5	0.0	1.31511184500197E-5	6.57555922500985E-5
26-27	1.7096453985025612E-4	9.20578291501379E-5	0.0	3.287779612504925E-5	6.57555922500985E-5
28-29	1.7096453985025612E-4	9.20578291501379E-5	0.0	1.9069121752528565E-4	6.57555922500985E-5
30-31	1.7754009907526596E-4	1.183600660501773E-4	0.0	7.233115147510835E-4	6.57555922500985E-5
32-33	1.9069121752528565E-4	1.183600660501773E-4	0.0	0.0019660922082779453	6.57555922500985E-5
34-35	1.9726677675029552E-4	1.5123786217522656E-4	0.0	0.0035442264222803094	7.89067107001182E-5
36-37	1.9726677675029552E-4	1.9726677675029552E-4	0.0	0.0058719743879337965	7.89067107001182E-5
38-39	1.9726677675029552E-4	1.9726677675029552E-4	0.0	0.009389898573314067	7.89067107001182E-5
40-41	1.9726677675029552E-4	2.104178952003152E-4	0.0	0.013026182824744513	9.20578291501379E-5
42-43	2.0384233597530536E-4	2.235690136503349E-4	0.0	0.016793978260675156	9.20578291501379E-5
44-45	2.104178952003152E-4	2.695979282254039E-4	0.0	0.02115357402685669	9.20578291501379E-5
46-47	2.104178952003152E-4	2.8932460590043343E-4	0.0	0.02579591883971364	9.20578291501379E-5
48-49	2.104178952003152E-4	2.9590016512544325E-4	0.0	0.030168665724345192	1.183600660501773E-4
50-51	2.104178952003152E-4	3.6165575737554174E-4	0.0	0.034817586096427156	1.183600660501773E-4
52-53	2.104178952003152E-4	3.682313166005516E-4	0.0	0.03880237498678313	1.31511184500197E-4
54-55	2.1699345442532507E-4	3.682313166005516E-4	0.0	0.04263592601496387	1.4466230295021672E-4
56-57	2.2356901365033492E-4	3.682313166005516E-4	0.0	0.047159910761770646	1.4466230295021672E-4
58-59	2.3014457287534476E-4	3.682313166005516E-4	0.0	0.05144059981725206	1.5123786217522656E-4
60-61	2.695979282254039E-4	3.682313166005516E-4	0.0	0.055951433445608816	1.578134214002364E-4
62-63	3.024757243504531E-4	3.682313166005516E-4	0.0	0.05990334453983974	1.7096453985025612E-4
64-65	3.024757243504531E-4	4.339869088506501E-4	0.0	0.0642234869506712	1.7754009907526596E-4
66-67	3.156268428004728E-4	4.339869088506501E-4	0.0	0.06891186067810323	1.9069121752528565E-4
68-69	3.287779612504925E-4	4.4056246807565997E-4	0.0	0.07352132769483513	1.9726677675029552E-4
70-71	3.287779612504925E-4	4.602891457506895E-4	0.0	0.07799270796784183	1.9726677675029552E-4
72-73	3.4192907970051223E-4	4.602891457506895E-4	0.0	0.08167502113384735	2.498712505503743E-4
74-75	3.4192907970051223E-4	4.602891457506895E-4	0.0	0.08435127373842635	2.498712505503743E-4
76-77	3.4192907970051223E-4	4.931669418757388E-4	0.0	0.08717218864595558	2.498712505503743E-4
78-79	3.4192907970051223E-4	5.26044738000788E-4	0.0	0.08904622302508339	2.498712505503743E-4
80-81	3.4192907970051223E-4	5.26044738000788E-4	0.0	0.08965117447378429	2.695979282254039E-4
82-83	3.4192907970051223E-4	5.26044738000788E-4	0.0	0.09007858582340994	3.024757243504531E-4
84-85	3.4192907970051223E-4	5.26044738000788E-4	0.0	0.09019694588946012	3.024757243504531E-4
86-87	3.4192907970051223E-4	5.391958564508078E-4	0.0	0.09032845707396031	3.024757243504531E-4
88	3.4192907970051223E-4	5.391958564508078E-4	0.0	0.09034818375163534	3.024757243504531E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11770	0.0	49.174675	1
CTTATAC	8085	0.0	48.530426	1
TATACAC	11225	0.0	37.60297	3
GTATCAA	21325	0.0	37.01796	1
TTATACA	10900	0.0	36.815224	2
ATCAACG	25550	0.0	30.198553	3
TCAACGC	25820	0.0	29.981049	4
CAACGCA	26040	0.0	29.627182	5
TATCAAC	26285	0.0	29.531828	2
AACGCAG	26745	0.0	28.858099	6
TACACAT	14430	0.0	28.718378	5
ACACATC	14515	0.0	27.230087	6
ACATCTC	15575	0.0	25.758478	8
ACGCAGA	30430	0.0	25.3495	7
CGCAGAG	31140	0.0	24.757895	8
ATACACA	18225	0.0	24.394703	4
CACATCT	16380	0.0	23.922373	7
GTGGTAT	5385	0.0	23.183752	1
GCAGAGT	35550	0.0	21.579182	9
CATCTCC	18490	0.0	21.307255	9
>>END_MODULE