FastQCFastQC Report
Fri 27 May 2016
SRR2138624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7504132
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC214650.067.348261
TTATACA253600.057.2619972
TATACAC262450.055.8290023
GGTATCA114700.047.8152661
TACACAT400750.036.813575
ATACACA422100.035.3509754
GTATCAA205950.035.2156071
ACACATC468700.030.9583686
ACATCTC499050.029.157438
ATCAACG244400.029.0403983
CACATCT502000.028.8640927
TCAACGC247750.028.6308574
TATCAAC251500.028.5128442
CAACGCA250350.028.3964885
AACGCAG253750.028.108816
CATCTCC561450.025.8295889
ACGCAGA287900.024.6895247
CGCAGAG291350.024.3831528
GCAGAGT327900.021.4660159
GTGGTAT58000.020.277691
Produced by FastQC (version 0.11.5)