##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138624_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7504132
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7920307105472	33.0	33.0	33.0	33.0	33.0
2	32.809169801384094	33.0	33.0	33.0	33.0	33.0
3	32.80369708315366	33.0	33.0	33.0	33.0	33.0
4	36.748078125491396	37.0	37.0	37.0	37.0	37.0
5	36.758476130217325	37.0	37.0	37.0	37.0	37.0
6	36.76586899057746	37.0	37.0	37.0	37.0	37.0
7	36.74849283035	37.0	37.0	37.0	37.0	37.0
8	36.751841385519334	37.0	37.0	37.0	37.0	37.0
9	36.757326363662045	37.0	37.0	37.0	37.0	37.0
10-11	36.731906501644694	37.0	37.0	37.0	37.0	37.0
12-13	36.71140546301691	37.0	37.0	37.0	37.0	37.0
14-15	38.98029512274037	40.0	40.0	40.0	37.0	40.0
16-17	38.91149156224864	40.0	40.0	40.0	37.0	40.0
18-19	38.94956378699095	40.0	40.0	40.0	37.0	40.0
20-21	38.94730795271164	40.0	40.0	40.0	37.0	40.0
22-23	38.98267414428534	40.0	40.0	40.0	37.0	40.0
24-25	38.99162834900709	40.0	40.0	40.0	37.0	40.0
26-27	38.86465933327565	40.0	40.0	40.0	37.0	40.0
28-29	38.770325050628664	40.0	37.0	40.0	37.0	40.0
30-31	38.703603680247625	40.0	37.0	40.0	37.0	40.0
32-33	38.683961249309874	40.0	37.0	40.0	37.0	40.0
34-35	38.66183980451474	40.0	37.0	40.0	37.0	40.0
36-37	38.61050932139399	40.0	37.0	40.0	37.0	40.0
38-39	38.50328363752519	40.0	37.0	40.0	37.0	40.0
40-41	38.503588376005524	40.0	37.0	40.0	37.0	40.0
42-43	38.47402107484993	40.0	37.0	40.0	37.0	40.0
44-45	38.38672604719363	40.0	37.0	40.0	37.0	40.0
46-47	38.30552834503679	40.0	37.0	40.0	37.0	40.0
48-49	38.2501668072371	40.0	37.0	40.0	37.0	40.0
50-51	38.06579442934289	40.0	37.0	40.0	37.0	40.0
52-53	38.0215216901454	40.0	37.0	40.0	37.0	40.0
54-55	37.965820683915155	40.0	37.0	40.0	35.0	40.0
56-57	37.82139350807864	40.0	37.0	40.0	33.0	40.0
58-59	37.73009373072058	40.0	37.0	40.0	33.0	40.0
60-61	37.50234485684777	40.0	37.0	40.0	33.0	40.0
62-63	37.34486037361042	37.0	37.0	40.0	33.0	40.0
64-65	37.18107534909497	37.0	37.0	40.0	33.0	40.0
66-67	36.992894109328915	37.0	37.0	40.0	33.0	40.0
68-69	36.79386731432433	37.0	37.0	40.0	33.0	40.0
70-71	36.55560303444999	37.0	37.0	40.0	33.0	40.0
72-73	36.329852386139066	37.0	37.0	37.0	33.0	40.0
74-75	36.10910476892096	37.0	37.0	37.0	33.0	40.0
76-77	34.508924414080994	35.0	33.0	37.0	30.0	37.0
78-79	35.745064260058555	37.0	37.0	37.0	33.0	37.0
80-81	35.86080181823792	37.0	37.0	37.0	33.0	37.0
82-83	35.80042212725178	37.0	37.0	37.0	33.0	37.0
84-85	35.681980364493214	37.0	37.0	37.0	33.0	37.0
86-87	35.578586788292725	37.0	37.0	37.0	33.0	37.0
88-89	35.513380709234	37.0	37.0	37.0	33.0	37.0
90-91	35.45713316776282	37.0	33.0	37.0	33.0	37.0
92-93	35.39471511114107	37.0	33.0	37.0	33.0	37.0
94-95	35.3613213246546	37.0	33.0	37.0	33.0	37.0
96-97	35.335172126786915	37.0	33.0	37.0	33.0	37.0
98-99	35.29465376638066	37.0	33.0	37.0	33.0	37.0
100	35.23761521785348	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	36.0
21	196.0
22	751.0
23	2219.0
24	4870.0
25	9637.0
26	16775.0
27	25496.0
28	37010.0
29	49822.0
30	64892.0
31	85193.0
32	113234.0
33	154585.0
34	223392.0
35	346768.0
36	688468.0
37	2173035.0
38	3343520.0
39	164226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.465941164147964	21.17212223878791	15.159234938831034	25.20270165823309
2	13.597735221075535	19.958883985516245	42.936531500245465	23.506849293162755
3	16.738683861063343	26.590915905461028	32.505671978726	24.164728254749637
4	11.557479735691038	19.30195086454832	36.214006029314945	32.92656337044569
5	12.768198585174991	37.46078149649013	35.64733388814576	14.123686030189116
6	28.951622919213044	37.4190645900152	18.282034484468024	15.347278006303727
7	24.92096354381826	33.45879310225353	21.10931684037541	20.5109265135528
8	24.550234457496217	35.76788094878928	21.70768051521482	17.974204078499685
9	23.791838949528074	16.54900260283268	19.735073956588185	39.92408449105106
10-11	23.405305237168005	26.163818813421724	28.33946018007146	22.091415769338813
12-13	24.810930831174076	23.569434812713848	27.774837915964163	23.84479644014791
14-15	22.438990945255227	25.622883499384074	24.549408246016995	27.388717309343708
16-17	22.322281910819267	29.601851353361052	25.1587858529141	22.917080882905577
18-19	23.09175798080311	26.955715864273177	26.925785687138763	23.026740467784947
20-21	23.120723935831386	26.55648069424492	26.867615585329386	23.45517978459431
22-23	22.062064369960684	27.517491837142664	27.52097021390018	22.899473578996467
24-25	22.860822826971866	27.203529990825782	27.322151652175535	22.613495530026817
26-27	22.972700063520488	27.7480346932302	27.75240322729956	21.526862015949753
28-29	21.657913073157626	26.340914231964973	28.405522215103378	23.59565047977402
30-31	21.820642442658915	26.501655624268366	29.074057928261897	22.603644004810818
32-33	20.814170831408287	25.83401317149444	30.470691464929228	22.881124532168045
34-35	20.775299080307217	26.606108035669152	29.411723582359883	23.206869301663748
36-37	21.55540090813254	26.793645531083527	29.198505279065866	22.45244828171807
38-39	22.045922330493315	25.912311298359793	28.896310514781202	23.145455856365693
40-41	21.589476123404623	25.80929633863944	29.83735015851569	22.763877379440245
42-43	22.496929997871227	25.364453282592827	30.233667011743222	21.90494970779273
44-45	21.39203213058086	26.500207849090536	29.46304465497121	22.644715365357396
46-47	23.21111411262282	25.205944347739955	27.769139710853537	23.813801828783692
48-49	22.337703370252733	25.30377129371789	28.81845467194639	23.54007066408298
50-51	21.755216504354948	26.44608481561371	28.95246556740414	22.846233112627207
52-53	21.612771729953792	27.39729021883644	29.702803981437658	21.28713406977211
54-55	21.472670816066483	27.69120905252094	28.124997936452417	22.711122194960158
56-57	22.81579494410758	28.598977798416264	26.549474658055765	22.03575259942039
58-59	21.054519085356535	29.787641015839718	28.493380949617308	20.66445894918644
60-61	22.426144717124483	30.80319092991707	25.897256526826467	20.873407826131977
62-63	21.283127135110583	31.521849178285986	25.933628900646944	21.261394785956483
64-65	21.590957443501583	30.517312110835487	26.403410417164736	21.488320028498194
66-67	21.999433941390908	29.335979135655993	26.70887430186657	21.95571262108653
68-69	22.256100820354945	28.15864143804694	27.254058545000564	22.331199196597552
70-71	22.371278971212718	27.583881529413283	27.439063111745952	22.605776387628048
72-73	22.50117867392074	27.56442786893929	27.410619728092257	22.523773729047715
74-75	22.3605227229907	27.704524140277663	27.383217944427727	22.551735192303905
76-77	22.46937449111407	27.634411819997638	27.289552663460885	22.606661025427403
78-79	22.408120908754682	27.64823638824953	27.284741697218223	22.658901005777565
80-81	22.445367706274418	27.595180355344834	27.374172537177376	22.58527940120337
82-83	22.53434528147739	27.507146772032115	27.486841563906943	22.47166638258355
84-85	22.694907637813998	27.455106534449193	27.339535932005838	22.51044989573098
86-87	22.443390900295366	27.473145399366572	27.468578482189777	22.614885218148288
88-89	22.578975629084137	27.364110438227296	27.395812226145377	22.661101706543185
90-91	22.62624777792313	27.384695234221883	27.45230879253687	22.536748195318125
92-93	22.675604250624946	27.302426727453486	27.464431177108008	22.557537844813563
94-95	22.48797275358961	27.399297649407934	27.489881368796443	22.622848228206017
96-97	22.565428040101505	27.52311542246137	27.540618317248494	22.370838220188634
98-99	23.730367396259673	29.400433418991216	27.71026766704818	19.158931517700932
100	22.630494796273485	30.401196249499673	21.470747017443244	25.497561936783598
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1094.0
1	1008.5
2	1521.0
3	2326.5
4	3451.0
5	5890.0
6	7835.0
7	9089.0
8	9923.5
9	9919.5
10	9365.0
11	8843.5
12	10133.0
13	13997.5
14	22352.5
15	33433.0
16	42343.5
17	47832.0
18	49048.5
19	46206.0
20	41427.0
21	36316.5
22	33113.0
23	33967.0
24	38810.0
25	47160.5
26	58537.5
27	71076.0
28	84171.5
29	99541.0
30	113938.0
31	127434.5
32	143151.5
33	158726.0
34	174826.0
35	190309.5
36	206178.0
37	222725.0
38	234058.0
39	240859.5
40	250331.5
41	259752.5
42	266673.0
43	275354.0
44	284258.0
45	294174.0
46	322995.5
47	370572.0
48	445250.0
49	462988.5
50	419782.5
51	406562.0
52	386192.5
53	336818.5
54	282940.5
55	246126.5
56	221902.0
57	200155.0
58	176691.5
59	152206.0
60	126081.5
61	101031.0
62	79502.0
63	61713.0
64	46801.5
65	34804.5
66	25737.5
67	19488.5
68	14846.0
69	10744.0
70	7526.5
71	5310.0
72	3674.5
73	2429.0
74	1559.0
75	979.0
76	651.5
77	427.5
78	276.5
79	186.0
80	125.0
81	75.5
82	53.0
83	40.0
84	29.5
85	21.0
86	12.0
87	8.5
88	7.0
89	5.5
90	4.5
91	3.5
92	2.5
93	2.0
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0018256608492494537
4	0.010554185347485891
5	0.017270485114067823
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.003712204328256966
26-27	0.042366827660611776
28-29	0.08907450766038764
30-31	0.08605685256915312
32-33	0.03544064626162471
34-35	0.0028299300119993065
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.0271870738778623E-4
56-57	2.0877038419119074E-4
58-59	3.038560548788344E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.441910108666265E-5
72-73	0.01187882175654033
74-75	0.024801641775558582
76-77	0.004910460897813398
78-79	0.0
80-81	0.0
82-83	0.005638487026796405
84-85	0.0441834379710683
86-87	0.04143276784321262
88-89	0.010601003701128897
90-91	1.6375959667359947E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	86.0
20-21	358.0
22-23	928.0
24-25	2100.0
26-27	3681.0
28-29	7919.0
30-31	18912.0
32-33	24415.0
34-35	32956.0
36-37	47816.0
38-39	55837.0
40-41	57093.0
42-43	66719.0
44-45	78481.0
46-47	75712.0
48-49	68311.0
50-51	62539.0
52-53	64651.0
54-55	98659.0
56-57	123996.0
58-59	119812.0
60-61	128199.0
62-63	136397.0
64-65	146470.0
66-67	162561.0
68-69	92417.0
70-71	62925.0
72-73	58397.0
74-75	60059.0
76-77	60365.0
78-79	62401.0
80-81	63014.0
82-83	60579.0
84-85	60780.0
86-87	63265.0
88-89	66917.0
90-91	70184.0
92-93	70571.0
94-95	78858.0
96-97	216612.0
98-99	402523.0
100-101	4369657.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.04851995816755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.39679257809242	76.03064897774057
2	7.416648756843462	12.615499996382393
3	1.8436237366485375	4.703924104851137
4	0.650352997306273	2.2124623948502657
5	0.291950193674628	1.2414965936763738
6	0.14504619449075248	0.740157850020181
7	0.08020015106039419	0.477463290367559
8	0.05208072330886741	0.3543510748616009
9	0.029867580681441293	0.22861741785189654
>10	0.09107005034940424	1.15607237477456
>50	0.0017548036383032066	0.09730554326493707
>100	5.631618637340271E-4	0.10080789885787826
>500	2.4536033368648458E-5	0.013543029070473984
>1k	2.4536008425573963E-5	0.027649453430159576
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3325991600360974E-5	0.0	0.0	0.0
3	0.0	1.3325991600360974E-5	0.0	0.0	0.0
4	0.0	3.9977974801082926E-5	0.0	0.0	0.0
5	0.0	3.9977974801082926E-5	0.0	1.3325991600360974E-5	0.0
6	1.3325991600360973E-4	3.9977974801082926E-5	0.0	1.3325991600360974E-5	0.0
7	1.3325991600360973E-4	5.3303966401443896E-5	0.0	1.3325991600360974E-5	0.0
8	1.4658590760397073E-4	5.3303966401443896E-5	0.0	1.3325991600360974E-5	0.0
9	1.4658590760397073E-4	1.1993392440324876E-4	0.0	1.3325991600360974E-5	1.3325991600360974E-5
10-11	1.4658590760397073E-4	1.2659692020342925E-4	0.0	5.996696220162438E-5	1.3325991600360974E-5
12-13	1.4658590760397073E-4	1.3325991600360973E-4	0.0	8.661894540234634E-5	1.3325991600360974E-5
14-15	1.4658590760397073E-4	1.8656388240505364E-4	0.0	1.1993392440324876E-4	1.3325991600360974E-5
16-17	1.8656388240505364E-4	1.9322687820523413E-4	0.0	1.665748950045122E-4	2.6651983200721948E-5
18-19	1.8656388240505364E-4	2.1321586560577559E-4	0.0	1.9988987400541461E-4	2.6651983200721948E-5
20-21	1.8656388240505364E-4	2.2654185720613656E-4	0.0	2.2654185720613656E-4	2.6651983200721948E-5
22-23	1.8656388240505364E-4	2.2654185720613656E-4	0.0	2.531938404068585E-4	2.6651983200721948E-5
24-25	1.9322687820523413E-4	2.531938404068585E-4	0.0	2.7984582360758044E-4	2.6651983200721948E-5
26-27	1.9988987400541461E-4	2.6651983200721947E-4	0.0	3.3981278580920486E-4	2.6651983200721948E-5
28-29	1.9988987400541461E-4	2.7318282780739995E-4	0.0	5.863436304158829E-4	3.331497900090244E-5
30-31	2.065528698055951E-4	2.9317181520794146E-4	0.0	0.001532489034041512	3.9977974801082926E-5
32-33	2.1321586560577559E-4	2.9317181520794146E-4	0.0	0.0032582049462882585	3.9977974801082926E-5
34-35	2.1321586560577559E-4	3.664647690099268E-4	0.0	0.005223788707341502	6.662995800180487E-5
36-37	2.1987886140595607E-4	4.3975772281191214E-4	0.0	0.008535297620031204	6.662995800180487E-5
38-39	2.3320485300631704E-4	4.530837144122731E-4	0.0	0.01379906430217379	6.662995800180487E-5
40-41	2.3986784880649753E-4	4.664097060126341E-4	0.0	0.018762996173308254	6.662995800180487E-5
42-43	2.46530844606678E-4	4.7307270181281457E-4	0.0	0.02283408660721853	6.662995800180487E-5
44-45	2.531938404068585E-4	5.730176388155218E-4	0.0	0.0278646484363548	7.329295380198537E-5
46-47	2.531938404068585E-4	6.596365842178682E-4	0.0	0.03339493495050461	7.995594960216585E-5
48-49	2.59856836207039E-4	7.062775548191317E-4	0.0	0.03893188446045459	9.328194120252682E-5
50-51	2.865088194077609E-4	7.995594960216585E-4	0.0	0.04389581633158905	9.328194120252682E-5
52-53	2.9317181520794146E-4	8.06222491821839E-4	0.0	0.04899300811872712	1.1993392440324878E-4
54-55	2.9317181520794146E-4	8.195484834221999E-4	0.0	0.054063547922664476	1.4658590760397073E-4
56-57	2.9317181520794146E-4	8.262114792223804E-4	0.0	0.05956051945781338	1.4658590760397073E-4
58-59	2.9317181520794146E-4	8.262114792223804E-4	0.0	0.06482428613995596	1.5324890340415122E-4
60-61	3.131608026084829E-4	8.728524498236437E-4	0.0	0.07026129071290324	1.599118992043317E-4
62-63	3.8645375641046826E-4	9.061674288245462E-4	0.0	0.07511861465123482	1.599118992043317E-4
64-65	3.8645375641046826E-4	9.994493700270731E-4	0.0	0.07978937470716133	1.599118992043317E-4
66-67	3.8645375641046826E-4	0.001012775361627434	0.0	0.08476663256989617	1.599118992043317E-4
68-69	3.8645375641046826E-4	0.0010261013532277949	0.0	0.0901769851596427	1.599118992043317E-4
70-71	3.9977974801082923E-4	0.0010460903406283365	0.0	0.09541409985858458	1.7323789080469267E-4
72-73	4.664097060126341E-4	0.0010594163322286973	0.0	0.09955848324629685	1.9988987400541461E-4
74-75	4.664097060126341E-4	0.001106057302829961	0.0	0.10262346131437985	1.9988987400541461E-4
76-77	4.7307270181281457E-4	0.0011460352776310437	0.0	0.10580837330686614	1.9988987400541461E-4
78-79	4.7973569761299505E-4	0.0011593612692314048	0.0	0.10785391301752155	1.9988987400541461E-4
80-81	4.7973569761299505E-4	0.0011593612692314048	0.0	0.10860016854714177	2.1321586560577559E-4
82-83	4.8639869341317554E-4	0.0011726872608317659	0.0	0.10917984918175747	2.531938404068585E-4
84-85	4.93061689213356E-4	0.0012060022398326683	0.0	0.10955297694656757	2.531938404068585E-4
86-87	4.93061689213356E-4	0.0012526432104339317	0.0	0.10965958487937047	2.531938404068585E-4
88	5.19713672414078E-4	0.0012792951936346536	0.0	0.10967291087097082	2.531938404068585E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	21465	0.0	67.34826	1
TTATACA	25360	0.0	57.261997	2
TATACAC	26245	0.0	55.829002	3
GGTATCA	11470	0.0	47.815266	1
TACACAT	40075	0.0	36.81357	5
ATACACA	42210	0.0	35.350975	4
GTATCAA	20595	0.0	35.215607	1
ACACATC	46870	0.0	30.958368	6
ACATCTC	49905	0.0	29.15743	8
ATCAACG	24440	0.0	29.040398	3
CACATCT	50200	0.0	28.864092	7
TCAACGC	24775	0.0	28.630857	4
TATCAAC	25150	0.0	28.512844	2
CAACGCA	25035	0.0	28.396488	5
AACGCAG	25375	0.0	28.10881	6
CATCTCC	56145	0.0	25.829588	9
ACGCAGA	28790	0.0	24.689524	7
CGCAGAG	29135	0.0	24.383152	8
GCAGAGT	32790	0.0	21.466015	9
GTGGTAT	5800	0.0	20.27769	1
>>END_MODULE