FastQCFastQC Report
Fri 27 May 2016
SRR2138623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8838139
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC121400.053.7043761
GGTATCA137200.049.024911
TATACAC154900.044.021533
TTATACA158300.041.8247452
GTATCAA255100.037.6836431
ATCAACG309000.030.455683
TCAACGC312600.030.1315634
TATCAAC318350.029.799482
CAACGCA315650.029.79215
AACGCAG322250.029.2593786
TACACAT233900.029.0727425
ACACATC241950.027.1467886
ATACACA262550.026.790294
ACATCTC249950.026.1767838
ACGCAGA364100.025.8152837
CGCAGAG367250.025.5938558
CACATCT263900.024.6813037
GTGGTAT65950.024.666671
TGGTATC66350.023.2476042
CATCTCC286450.022.67959
Produced by FastQC (version 0.11.5)