FastQCFastQC Report
Fri 27 May 2016
SRR2138622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7882937
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC128750.054.2112051
GGTATCA132550.044.43331
TATACAC163100.044.1656383
TTATACA164550.042.7188342
GTATCAA231300.034.9424361
ATACACA213450.034.852364
ACACATC223350.031.3689736
ATCAACG261800.030.3329433
TACACAT243200.029.7121335
CAACGCA275350.028.7709185
TCAACGC276400.028.7440514
AACGCAG277750.028.5963556
ACGCAGA280850.028.324977
TATCAAC283150.028.3240832
ACATCTC262850.026.7810788
CACATCT273700.025.5074927
CGCAGAG322700.024.625918
CTCGTAT371000.023.251429
CATCTCC301900.023.1111559
TCTCGTA368650.022.8489278
Produced by FastQC (version 0.11.5)