##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138622_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7882937
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.849114866705136	33.0	33.0	33.0	33.0	33.0
2	32.91157788017334	33.0	33.0	33.0	33.0	33.0
3	33.60810926181448	33.0	33.0	33.0	33.0	37.0
4	36.728640226352184	37.0	37.0	37.0	37.0	37.0
5	36.73375050441225	37.0	37.0	37.0	37.0	37.0
6	36.74680693249229	37.0	37.0	37.0	37.0	37.0
7	36.742763008254414	37.0	37.0	37.0	37.0	37.0
8	36.63678639065617	37.0	37.0	37.0	37.0	37.0
9	36.64599831255787	37.0	37.0	37.0	37.0	37.0
10-11	36.68481753691549	37.0	37.0	37.0	37.0	37.0
12-13	36.94626019464573	37.0	37.0	37.0	37.0	38.5
14-15	38.89730122161322	40.0	38.5	40.0	37.0	40.0
16-17	38.846360563328105	40.0	37.0	40.0	37.0	40.0
18-19	38.88872567419986	40.0	40.0	40.0	37.0	40.0
20-21	38.899198809113315	40.0	40.0	40.0	37.0	40.0
22-23	38.96249939906315	40.0	40.0	40.0	37.0	40.0
24-25	38.94513997726293	40.0	40.0	40.0	37.0	40.0
26-27	38.81019374675068	40.0	38.5	40.0	37.0	40.0
28-29	38.74412997935667	40.0	37.0	40.0	37.0	40.0
30-31	38.60626739539171	40.0	37.0	40.0	37.0	40.0
32-33	38.58083118331693	40.0	37.0	40.0	37.0	40.0
34-35	38.589026359924205	40.0	37.0	40.0	37.0	40.0
36-37	38.56870084324367	40.0	37.0	40.0	37.0	40.0
38-39	38.490720623073074	40.0	37.0	40.0	37.0	40.0
40-41	38.49101534148592	40.0	37.0	40.0	37.0	40.0
42-43	38.40691943997736	40.0	37.0	40.0	37.0	40.0
44-45	38.365276770646545	40.0	37.0	40.0	37.0	40.0
46-47	38.30637492576352	40.0	37.0	40.0	37.0	40.0
48-49	38.25597489782403	40.0	37.0	40.0	37.0	40.0
50-51	38.15676086249744	40.0	37.0	40.0	37.0	40.0
52-53	38.1094108405286	40.0	37.0	40.0	37.0	40.0
54-55	38.03849153283127	40.0	37.0	40.0	37.0	40.0
56-57	37.90098204690364	40.0	37.0	40.0	33.0	40.0
58-59	37.68840849029449	40.0	37.0	40.0	33.0	40.0
60-61	37.56933938217952	40.0	37.0	40.0	33.0	40.0
62-63	37.258066003912205	37.0	37.0	40.0	33.0	40.0
64-65	37.09936666475653	37.0	37.0	40.0	33.0	40.0
66-67	36.96692342357129	37.0	37.0	40.0	33.0	40.0
68-69	36.760571711290766	37.0	37.0	40.0	33.0	40.0
70-71	36.508027879590955	37.0	37.0	40.0	33.0	40.0
72-73	36.276759616315466	37.0	37.0	37.0	33.0	40.0
74-75	35.81505205855692	37.0	35.0	37.0	33.0	40.0
76-77	34.479952215022266	35.0	33.0	37.0	30.0	37.0
78-79	35.66845906074055	37.0	35.0	37.0	33.0	37.0
80-81	35.77777003014441	37.0	37.0	37.0	33.0	37.0
82-83	35.677592386683116	37.0	37.0	37.0	33.0	37.0
84-85	35.29810394249698	37.0	35.0	37.0	33.0	37.0
86-87	35.40092323950292	37.0	33.0	37.0	33.0	37.0
88-89	35.40648668353671	37.0	33.0	37.0	33.0	37.0
90-91	35.35249136024675	37.0	33.0	37.0	33.0	37.0
92-93	35.28247571705895	37.0	33.0	37.0	33.0	37.0
94-95	35.24640333632509	37.0	33.0	37.0	33.0	37.0
96-97	35.20326954697441	37.0	33.0	37.0	33.0	37.0
98-99	35.15433605397145	37.0	33.0	37.0	33.0	37.0
100	35.06320815166185	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	5.0
17	6.0
18	8.0
19	12.0
20	56.0
21	175.0
22	663.0
23	1924.0
24	4413.0
25	8621.0
26	15246.0
27	24567.0
28	36988.0
29	52253.0
30	71761.0
31	97442.0
32	134338.0
33	185384.0
34	268123.0
35	414081.0
36	789487.0
37	2233348.0
38	3368637.0
39	175399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.004637252199416	0.0	25.226639279118185	29.768723468682406
2	14.390000459225238	24.01767027678596	38.05758126320431	23.53474800078449
3	16.003641539276014	24.97702153702035	33.24301375434656	25.776323169357074
4	13.92457409223572	24.349941941711283	35.9586431604722	25.766840805580806
5	16.804847630512032	36.32339559437097	32.374534412292924	14.497222362824076
6	27.159217433806717	38.683970200446865	19.383245102681908	14.773567263064516
7	25.141479628722134	33.61559276701057	22.37820751326568	18.864720091001615
8	23.734973398874047	31.70285389823615	22.579807500681532	21.98236520220826
9	22.860920999368638	20.331013681829504	23.3007443799183	33.507320938883566
10-11	24.030427999107438	25.782465342549354	28.349731832183867	21.837374826159337
12-13	25.27342283719888	23.720866981430905	28.380798933189492	22.624911248180723
14-15	22.141100455325216	26.585294288156813	26.252340973928877	25.02126428258909
16-17	22.05381065458217	28.85609767019577	26.430415465707767	22.659676209514295
18-19	22.02976504823012	27.715881783655	28.286557408742453	21.96779575937243
20-21	22.358146138151483	26.763600810236472	28.862904634578506	22.01534841703354
22-23	21.903275618407285	27.099693335402208	29.36641807604536	21.630612970145148
24-25	22.175466427233225	27.378892313647228	28.88828113969894	21.5573601194206
26-27	22.77058046863411	27.68734467427064	28.001539540875942	21.54053531621931
28-29	21.05915544460337	27.593418197812998	29.68381190735036	21.663614450233275
30-31	23.128557582412657	27.606151943657263	28.331515494455573	20.933774979474506
32-33	21.108451950910265	29.01433618015518	28.39344513289156	21.483766736043
34-35	21.580290077165447	28.509397820981203	28.821518234640592	21.088793867212758
36-37	21.352580889397007	28.716899527471323	28.66022906924586	21.270290513885808
38-39	21.540235817431306	27.568583657556623	29.275012643942727	21.616167881069345
40-41	21.811197355025477	26.968019564647435	29.062403812830556	22.15837926749653
42-43	22.314682866140426	26.73112053928241	29.08987579008087	21.86432080449629
44-45	21.78962529772371	27.501152820056053	28.87236235543415	21.836859526786085
46-47	22.20401264613886	26.71113383613119	28.219507043821164	22.865346473908787
48-49	22.148768884512688	26.882916278389104	28.733590810339706	22.234724026758506
50-51	21.814335832750324	27.475141239578253	28.16135269161087	22.549170236060554
52-53	21.934153168375012	27.550241404548736	28.59475343775874	21.92085198931751
54-55	21.8734026392329	27.877042538554477	28.21954973737974	22.030005084832887
56-57	22.514498053298343	28.569188858430373	26.949527960932578	21.966785127338706
58-59	21.76250154377365	28.614445518248772	28.153501336209864	21.469551601767716
60-61	22.312856726198515	29.10793929630596	27.092620567504877	21.48658340999064
62-63	21.898355705156693	29.424494957611575	26.98331912812141	21.693830209110327
64-65	21.96608655699846	28.91862400561349	27.17254249104953	21.94274694633852
66-67	22.091581176684464	28.551039613208467	27.299040970894417	22.058338239212652
68-69	22.20921010518215	28.121022614058283	27.564089262077196	22.10567801868237
70-71	22.268289367699186	27.65341182164263	27.640193706601984	22.4381051040562
72-73	22.285914841249067	27.79769796264465	27.658285542213832	22.258101653892446
74-75	22.16940837858682	27.974374468288104	27.61013095020394	22.246086202921138
76-77	22.45613221039199	27.767269815073682	27.380329174096076	22.396268800438254
78-79	22.19873427821359	27.85712594489647	27.54059819495826	22.40354158193168
80-81	22.24149079067766	27.91278931565709	27.54512601952409	22.300593874141157
82-83	22.430266749238886	27.61827120734378	27.654080476114206	22.297381567303123
84-85	22.512497618345222	27.63955157584344	27.576498416639065	22.271452389172268
86-87	22.294607767191888	27.781439221089304	27.634168252406788	22.289784759312024
88-89	22.43892083594092	27.54850113535961	27.571064578679678	22.44151345001979
90-91	22.444368196181603	27.5950499997358	27.638526524361534	22.322055279721063
92-93	22.390263423586227	27.657401495498352	27.672317346845578	22.280017734069848
94-95	22.401227505476314	27.547617534948476	27.6808126279875	22.37034233158771
96-97	22.36763198666538	27.868025575978756	27.820106793809344	21.944235643546513
98-99	23.53018016761338	29.891465547968405	27.291787294913515	19.2865669895047
100	22.68128437781074	30.19094797412941	21.669901391769137	25.45786625629071
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1141.0
1	1069.5
2	1721.5
3	2665.5
4	3907.5
5	6682.5
6	8819.5
7	10232.5
8	11232.5
9	11226.5
10	10640.5
11	9977.5
12	11128.0
13	14561.0
14	23065.0
15	36203.5
16	47940.0
17	54922.0
18	55875.0
19	52063.0
20	46260.0
21	40359.0
22	36781.0
23	37266.0
24	41783.5
25	50605.0
26	63562.0
27	77979.5
28	92958.5
29	110384.5
30	127541.0
31	143591.5
32	161501.5
33	178637.5
34	196644.5
35	216151.0
36	241993.0
37	276787.5
38	305656.5
39	323844.5
40	333834.0
41	333265.0
42	322588.5
43	312949.0
44	314287.5
45	329599.0
46	358884.5
47	381875.0
48	380398.5
49	370314.5
50	360536.5
51	337602.5
52	306229.0
53	279328.5
54	259540.5
55	240632.5
56	219997.5
57	197489.5
58	173388.0
59	150336.0
60	125354.5
61	100429.5
62	78604.5
63	60454.5
64	45813.5
65	34184.5
66	25715.5
67	19774.0
68	14999.0
69	10710.5
70	7490.0
71	5189.0
72	3481.0
73	2278.5
74	1553.0
75	1078.5
76	688.5
77	449.5
78	315.5
79	224.0
80	149.0
81	97.0
82	67.0
83	44.5
84	28.5
85	17.0
86	11.0
87	8.5
88	8.0
89	7.0
90	5.0
91	3.5
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.566825790945684E-4
2	0.0012051345837217778
3	0.007053208721571669
4	0.026183134534755254
5	0.0020043290971372726
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.7087462369054845E-5
22-23	2.7277608984356295E-4
24-25	0.006884294987916001
26-27	0.04346087718103369
28-29	0.08656780655763362
30-31	0.07782675436224874
32-33	0.031064563655442648
34-35	0.0015616016990226485
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.1095969361901777E-5
56-57	3.5550987332685495E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	1.5428369052450514E-5
70-71	9.357869980350813E-4
72-73	0.012377383591179745
74-75	0.020159245319255354
76-77	0.002665163257706115
78-79	1.621721993063895E-5
80-81	4.342783612858376E-4
82-83	0.008628593535941321
84-85	0.03971474337464191
86-87	0.03648923487217137
88-89	0.008213548144995819
90-91	1.7324772701147755E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	174.0
20-21	588.0
22-23	1132.0
24-25	2959.0
26-27	4457.0
28-29	9537.0
30-31	18464.0
32-33	24772.0
34-35	39817.0
36-37	84469.0
38-39	91490.0
40-41	72206.0
42-43	67697.0
44-45	78483.0
46-47	75350.0
48-49	65168.0
50-51	59299.0
52-53	59889.0
54-55	72252.0
56-57	86361.0
58-59	84689.0
60-61	91801.0
62-63	94978.0
64-65	88564.0
66-67	107139.0
68-69	73573.0
70-71	62536.0
72-73	60159.0
74-75	61339.0
76-77	61319.0
78-79	63960.0
80-81	64385.0
82-83	63293.0
84-85	63226.0
86-87	66116.0
88-89	70626.0
90-91	73083.0
92-93	77060.0
94-95	99294.0
96-97	345584.0
98-99	1314330.0
100-101	3881319.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.78574911391594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.85503423107924	72.73144822248622
2	8.314186873710803	13.765923772264715
3	2.1674908489334674	5.3831206087954415
4	0.7972449132862753	2.6400206949465304
5	0.3578048483309641	1.4810571202834968
6	0.18507964442306685	0.919317420558047
7	0.10422336786129144	0.603974670850057
8	0.06461659426162764	0.4279466528911045
9	0.03966459162612672	0.2955296637959811
>10	0.11160795991644455	1.4164649506106466
>50	0.0021371537521022222	0.11883783321531523
>100	8.313270780707056E-4	0.15267872470608806
>500	5.1763827696308924E-5	0.033425396570395075
>1k	2.5881912839536035E-5	0.030254268026059628
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2685627197071346E-5
2	0.0	1.395418991677848E-4	0.0	0.0	1.2685627197071346E-5
3	0.0	1.395418991677848E-4	0.0	2.5371254394142692E-5	1.2685627197071346E-5
4	0.0	1.395418991677848E-4	0.0	3.8056881591214035E-5	1.2685627197071346E-5
5	0.0	1.395418991677848E-4	0.0	3.8056881591214035E-5	1.2685627197071346E-5
6	7.611376318242807E-5	1.395418991677848E-4	0.0	3.8056881591214035E-5	2.5371254394142692E-5
7	7.611376318242807E-5	1.395418991677848E-4	0.0	3.8056881591214035E-5	2.5371254394142692E-5
8	7.611376318242807E-5	1.5222752636485614E-4	0.0	3.8056881591214035E-5	2.5371254394142692E-5
9	7.611376318242807E-5	2.790837983355696E-4	0.0	3.8056881591214035E-5	5.0742508788285384E-5
10-11	8.245657678096374E-5	2.790837983355696E-4	0.0	5.7085322386821055E-5	5.0742508788285384E-5
12-13	8.879939037949941E-5	2.9176942553264096E-4	0.0	7.611376318242807E-5	5.0742508788285384E-5
14-15	8.879939037949941E-5	4.2496851110189005E-4	0.0	8.245657678096374E-5	7.611376318242807E-5
16-17	1.2685627197071345E-4	4.5668257909456844E-4	0.0	1.0148501757657077E-4	8.879939037949941E-5
18-19	1.2685627197071345E-4	4.5668257909456844E-4	0.0	1.7759878075899885E-4	8.879939037949941E-5
20-21	1.2685627197071345E-4	4.5668257909456844E-4	0.0	2.663981711384983E-4	8.879939037949941E-5
22-23	1.3319908556924914E-4	4.947394606857825E-4	0.0	3.361691207223906E-4	1.0782783117510643E-4
24-25	1.395418991677848E-4	5.708532238682105E-4	0.0	4.2496851110189005E-4	1.3319908556924914E-4
26-27	1.395418991677848E-4	6.215957326564959E-4	0.0	5.708532238682105E-4	1.395418991677848E-4
28-29	1.395418991677848E-4	6.279385462550316E-4	0.0	0.0010846211253496	1.395418991677848E-4
30-31	1.395418991677848E-4	6.97709495838924E-4	0.0	0.002283412895472842	1.395418991677848E-4
32-33	1.395418991677848E-4	6.97709495838924E-4	0.0	0.004522426095755935	1.4588471276632045E-4
34-35	1.395418991677848E-4	8.435942086052444E-4	0.0	0.007757261031009127	1.7759878075899882E-4
36-37	1.395418991677848E-4	0.0010021645485686363	0.0	0.012488999975516741	1.7759878075899882E-4
38-39	1.395418991677848E-4	0.001065592684553993	0.0	0.018825470760453877	1.7759878075899882E-4
40-41	1.395418991677848E-4	0.0011924489565247065	0.0	0.025409311275733905	1.7759878075899882E-4
42-43	1.7759878075899882E-4	0.0012875911605027416	0.0	0.03171406799267836	1.7759878075899882E-4
44-45	1.7759878075899882E-4	0.0013827333644807766	0.0	0.03902733207178999	1.7759878075899882E-4
46-47	1.7759878075899882E-4	0.0013827333644807766	0.0	0.04685436405238301	1.7759878075899882E-4
48-49	1.9662722155460585E-4	0.001395418991677848	0.0	0.054649681964983356	1.7759878075899882E-4
50-51	2.2199847594874856E-4	0.001490561195655883	0.0	0.06205808824807302	1.7759878075899882E-4
52-53	2.2834128954728422E-4	0.0015222752636485615	0.0	0.06968849300711144	1.839415943575345E-4
54-55	2.2834128954728422E-4	0.0015286180772470972	0.0	0.07641187542155925	1.902844079560702E-4
56-57	2.2834128954728422E-4	0.0015476465180427042	0.0	0.0816129825723585	1.9662722155460588E-4
58-59	2.2834128954728422E-4	0.0015476465180427042	0.0	0.08667454782398996	2.1565566235021288E-4
60-61	2.663981711384983E-4	0.0015603321452397755	0.0	0.09146971490448294	2.2834128954728422E-4
62-63	3.1714067992678365E-4	0.0015857033996339181	0.0	0.09618876822179348	2.2834128954728422E-4
64-65	3.2982630712385496E-4	0.0019282153339548445	0.0	0.10109810594706009	2.3468410314581988E-4
66-67	3.4251193432092633E-4	0.0019282153339548445	0.0	0.10657829689619491	2.4102691674435556E-4
68-69	3.4251193432092633E-4	0.0020233575379328796	0.0	0.11151300587585566	2.4102691674435556E-4
70-71	3.551975615179977E-4	0.002067757233122629	0.0	0.11621303075237059	2.4102691674435556E-4
72-73	4.439969518974971E-4	0.002086785673918236	0.0	0.11976500636755058	2.790837983355696E-4
74-75	4.439969518974971E-4	0.002112156928312379	0.0	0.12265732936848284	2.790837983355696E-4
76-77	4.439969518974971E-4	0.0021692422506992	0.0	0.12544182453824	2.790837983355696E-4
78-79	4.439969518974971E-4	0.0022199847594874854	0.0	0.12714804139624608	2.790837983355696E-4
80-81	4.439969518974971E-4	0.0022199847594874854	0.0	0.1276998661793187	2.8542661193410525E-4
82-83	4.503397654960328E-4	0.0022199847594874854	0.0	0.12813752031761766	2.9176942553264096E-4
84-85	4.8205383348871113E-4	0.0022390132002830924	0.0	0.1283214619119752	2.981122391311766E-4
86-87	4.8205383348871113E-4	0.0022960985226699133	0.0	0.12839123286155907	3.044550527297123E-4
88	4.8205383348871113E-4	0.0023341554042611277	0.0	0.12840391848875615	3.044550527297123E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	12875	0.0	54.211205	1
GGTATCA	13255	0.0	44.4333	1
TATACAC	16310	0.0	44.165638	3
TTATACA	16455	0.0	42.718834	2
GTATCAA	23130	0.0	34.942436	1
ATACACA	21345	0.0	34.85236	4
ACACATC	22335	0.0	31.368973	6
ATCAACG	26180	0.0	30.332943	3
TACACAT	24320	0.0	29.712133	5
CAACGCA	27535	0.0	28.770918	5
TCAACGC	27640	0.0	28.744051	4
AACGCAG	27775	0.0	28.596355	6
ACGCAGA	28085	0.0	28.32497	7
TATCAAC	28315	0.0	28.324083	2
ACATCTC	26285	0.0	26.781078	8
CACATCT	27370	0.0	25.507492	7
CGCAGAG	32270	0.0	24.62591	8
CTCGTAT	37100	0.0	23.25142	9
CATCTCC	30190	0.0	23.111155	9
TCTCGTA	36865	0.0	22.848927	8
>>END_MODULE