FastQCFastQC Report
Fri 27 May 2016
SRR2138621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8623013
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138250.050.7782141
GTATCAA252500.037.1420441
ATCAACG308050.030.0478533
TATCAAC316750.029.4086762
CAACGCA315150.029.3201035
TCAACGC316650.029.2576924
AACGCAG323600.028.7225996
ACGCAGA367000.025.1998047
CGCAGAG369850.024.9942388
GTGGTAT66200.022.6463891
TGGTATC65200.022.2832472
GCAGAGT425550.021.702979
GAGTACT262200.020.36530712-13
GTACATG286950.019.929691
TACATGG281700.019.6881962
CAGAGTA416200.019.35350210-11
ACATGGG289050.018.8670393
AGAGTAC391400.018.40188610-11
GTACTTT298600.017.82680714-15
AGTACTT282150.017.1424612-13
Produced by FastQC (version 0.11.5)