##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138621_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8623013
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.796182610417034	33.0	33.0	33.0	33.0	33.0
2	32.79631295928697	33.0	33.0	33.0	33.0	33.0
3	32.796255902664186	33.0	33.0	33.0	33.0	33.0
4	36.736304699992914	37.0	37.0	37.0	37.0	37.0
5	36.75770534034913	37.0	37.0	37.0	37.0	37.0
6	36.76889423685201	37.0	37.0	37.0	37.0	37.0
7	36.755683889146404	37.0	37.0	37.0	37.0	37.0
8	36.7515559816505	37.0	37.0	37.0	37.0	37.0
9	36.744598204827014	37.0	37.0	37.0	37.0	37.0
10-11	36.701164604529765	37.0	37.0	37.0	37.0	37.0
12-13	36.7026975373921	37.0	37.0	37.0	37.0	37.0
14-15	38.9637225410654	40.0	40.0	40.0	37.0	40.0
16-17	38.91233302095219	40.0	40.0	40.0	37.0	40.0
18-19	38.946083984797426	40.0	40.0	40.0	37.0	40.0
20-21	38.9929420802971	40.0	40.0	40.0	37.0	40.0
22-23	39.024618577385894	40.0	40.0	40.0	37.0	40.0
24-25	39.03256339439986	40.0	40.0	40.0	37.0	40.0
26-27	38.9401401019771	40.0	40.0	40.0	37.0	40.0
28-29	38.86789852915163	40.0	40.0	40.0	37.0	40.0
30-31	38.78973058814599	40.0	37.0	40.0	37.0	40.0
32-33	38.73380967379773	40.0	37.0	40.0	37.0	40.0
34-35	38.73392892681396	40.0	37.0	40.0	37.0	40.0
36-37	38.699256515224455	40.0	37.0	40.0	37.0	40.0
38-39	38.664687718477964	40.0	37.0	40.0	37.0	40.0
40-41	38.61791908067658	40.0	37.0	40.0	37.0	40.0
42-43	38.5020600458061	40.0	37.0	40.0	37.0	40.0
44-45	38.46697143917714	40.0	37.0	40.0	37.0	40.0
46-47	38.404275300254184	40.0	37.0	40.0	37.0	40.0
48-49	38.33723337099673	40.0	37.0	40.0	37.0	40.0
50-51	38.29649136020806	40.0	37.0	40.0	37.0	40.0
52-53	38.26395095505332	40.0	37.0	40.0	37.0	40.0
54-55	38.20244357000129	40.0	37.0	40.0	37.0	40.0
56-57	38.10928317285125	40.0	37.0	40.0	37.0	40.0
58-59	37.86048213417013	40.0	37.0	40.0	33.0	40.0
60-61	37.8026991689718	40.0	37.0	40.0	33.0	40.0
62-63	37.470862603012094	38.5	37.0	40.0	33.0	40.0
64-65	37.29408957351548	37.0	37.0	40.0	33.0	40.0
66-67	37.16140947957665	37.0	37.0	40.0	33.0	40.0
68-69	36.94554458729911	37.0	37.0	40.0	33.0	40.0
70-71	36.68237672819373	37.0	37.0	40.0	33.0	40.0
72-73	36.45211586390265	37.0	37.0	38.5	33.0	40.0
74-75	36.20122877396175	37.0	37.0	37.0	33.0	40.0
76-77	34.393376798938746	35.0	33.0	37.0	30.0	37.0
78-79	35.74296044067385	37.0	35.0	37.0	33.0	37.0
80-81	35.86928147836419	37.0	37.0	37.0	33.0	37.0
82-83	35.80362397147201	37.0	37.0	37.0	33.0	37.0
84-85	35.367902989548845	37.0	37.0	37.0	33.0	37.0
86-87	35.45746552240428	37.0	33.0	37.0	33.0	37.0
88-89	35.46016695115951	37.0	33.0	37.0	33.0	37.0
90-91	35.40406517290855	37.0	33.0	37.0	33.0	37.0
92-93	35.33027317691329	37.0	33.0	37.0	33.0	37.0
94-95	35.28666983108939	37.0	33.0	37.0	33.0	37.0
96-97	35.244909792283906	37.0	33.0	37.0	33.0	37.0
98-99	35.20534981095319	37.0	33.0	37.0	33.0	37.0
100	35.137524455530034	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	5.0
20	32.0
21	104.0
22	443.0
23	1279.0
24	3172.0
25	6847.0
26	12761.0
27	21653.0
28	33642.0
29	49558.0
30	70012.0
31	97541.0
32	134687.0
33	187591.0
34	270943.0
35	420250.0
36	813880.0
37	2380197.0
38	3926084.0
39	192329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.123816002596776	20.978490928866744	14.893170171493422	23.004522897043064
2	14.41745478059699	21.272320939328285	42.159022606135466	22.151201673939262
3	17.72449017696379	26.81301660440219	31.003094784651186	24.459398433982837
4	12.183861669998763	18.62672991966078	39.78663831155618	29.402770098784277
5	12.981105660183925	38.00714710336348	35.77985279481338	13.23189444163922
6	28.747121220854012	38.60713186910422	19.27199924202828	13.373747668013488
7	25.61133793953459	33.456774331663425	23.6342911694555	17.297596559346484
8	24.0510480501421	36.541914061825025	21.624402050652133	17.782635837380738
9	25.45098795513819	17.073614524296783	22.01432376363111	35.461073756933914
10-11	23.710546418055962	27.272288700017032	29.101701458643287	19.915463423283715
12-13	24.650629658102105	25.015600695487763	29.290916063793482	21.042853582616655
14-15	21.876645668979045	26.31388239818263	27.345807086223807	24.46366484661452
16-17	20.90846900033666	29.420395168139024	28.092495047844647	21.578640783679674
18-19	20.83688729217966	28.45605126653526	30.193541399044626	20.513520042240454
20-21	21.84318356367571	27.48580588616122	30.13019763471543	20.540812915447642
22-23	21.605749046078195	27.49039119492479	29.901401018289786	21.002458740707237
24-25	21.314977066653242	27.664771605836552	30.01379173385683	21.00645959365338
26-27	21.259670370634982	27.85083573905842	29.84750679320739	21.041987097099206
28-29	21.040215591806156	27.9602043348891	29.947686940581885	21.051893132722864
30-31	21.41648491047451	27.71782124311463	29.883975409282993	20.98171843712787
32-33	21.28510196774293	27.88713568129707	29.65700752577983	21.170754825180172
34-35	21.311923388072586	28.00439844883148	29.739542799524088	20.94413536357185
36-37	21.376945230373874	27.90959547872901	29.594581735294216	21.118877555602896
38-39	21.2766115817602	27.97877394464902	29.636720577165715	21.107893896425065
40-41	21.512062908800793	27.891293432299634	29.49027047350043	21.106373185399143
42-43	21.450014199361433	27.976352590386355	29.218009528416268	21.35562368183594
44-45	21.56678041171966	28.195490065282115	28.91990534793151	21.31782417506671
46-47	21.613734910945663	28.176070739682203	28.568011253817595	21.642183095554536
48-49	21.714187645443246	28.25714393441423	28.481594044645718	21.547074375496805
50-51	21.68649056004829	28.353211383244197	28.335939616303072	21.624358440404436
52-53	21.728138152739014	28.376524445483597	28.22210969421758	21.673227707559814
54-55	21.794717681491377	28.349427554202023	28.141742211195737	21.714112553110866
56-57	21.861688241154173	28.406239952973422	28.078607839726722	21.653463966145686
58-59	21.753756386624804	28.467839625424237	28.21505617043073	21.56334781752023
60-61	21.83866127974798	28.495466980627544	28.10202647554246	21.563845264082016
62-63	21.859517578136213	28.474356181678058	27.94216290648044	21.72396333370529
64-65	21.792767743432478	28.473445283081965	28.018537148410505	21.71524982507505
66-67	21.887905827078725	28.409473773050877	27.91174573031274	21.79087466955766
68-69	21.804159349680212	28.33088632627329	28.017552876847702	21.847401447198795
70-71	21.76228495629316	28.16611801966825	28.067140203815384	22.004456820223204
72-73	21.907784990475683	28.20754593788411	28.029979095400986	21.854689976239218
74-75	21.84823885672381	28.295345880801857	27.975138650481746	21.881276611992586
76-77	21.902246628695156	28.248040710995507	27.89286638363934	21.956846276669992
78-79	21.836441114067352	28.22446546580072	27.933007279320393	22.006086140811536
80-81	21.807067360382064	28.22217505404861	28.003602056316428	21.967155529252892
82-83	21.946127659636893	28.141006691865382	28.115539020477563	21.797326628020162
84-85	22.10840959187763	28.057652201663956	27.89568721750941	21.938250988949004
86-87	21.849597243156573	28.13734713146846	28.041117747772827	21.97193787760214
88-89	22.019741762484724	27.988976109837594	28.00438249797824	21.98689962969944
90-91	22.038677218901775	27.98802790766604	28.0223047502466	21.95099012318558
92-93	22.04262049818313	27.937393381977937	28.121353157939705	21.898632961899224
94-95	21.875751614175023	27.987589822227772	28.1585982680432	21.978060295554013
96-97	21.948913940846676	28.135352298305637	28.17534291896637	21.740390841881318
98-99	23.044929938340985	30.034603443074047	28.40559689191301	18.514869726671954
100	22.038761157621206	30.936558765072338	22.211520784113578	24.81315929319288
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1419.0
1	1361.5
2	2209.0
3	3374.0
4	4933.5
5	8411.5
6	11160.0
7	12847.5
8	13840.0
9	13588.0
10	12641.0
11	11990.5
12	13821.0
13	18895.0
14	30115.5
15	45401.5
16	58060.5
17	65724.5
18	67296.5
19	63721.5
20	57737.0
21	51413.5
22	47735.0
23	49587.0
24	56943.0
25	69178.0
26	86096.0
27	104947.0
28	125194.5
29	147991.0
30	169279.5
31	190496.5
32	212887.0
33	232944.0
34	252903.5
35	271613.0
36	290248.5
37	308599.0
38	319696.5
39	326676.0
40	334839.0
41	339883.5
42	342622.5
43	346322.0
44	349848.0
45	355066.0
46	358917.5
47	358588.0
48	352094.0
49	339988.5
50	328429.0
51	315173.0
52	298130.0
53	279810.0
54	261927.0
55	240800.5
56	216973.0
57	192267.0
58	167082.5
59	143880.0
60	119464.5
61	95982.5
62	76079.0
63	58421.5
64	43014.5
65	31042.5
66	22831.0
67	17316.5
68	12988.5
69	9256.5
70	6380.5
71	4405.0
72	3049.0
73	2064.0
74	1414.0
75	928.5
76	607.0
77	395.0
78	252.5
79	172.5
80	125.5
81	89.0
82	64.5
83	45.0
84	25.5
85	15.0
86	13.0
87	10.5
88	7.5
89	4.5
90	2.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0013800280713945345
4	0.03264520185693794
5	0.002539715526347925
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.8992717609190925E-5
22-23	0.0
24-25	0.003404462827383759
26-27	0.040987068391376204
28-29	0.08810806917020356
30-31	0.0859161501846619
32-33	0.03946189678319713
34-35	0.002495337459035851
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	5.0854944724807055E-5
56-57	1.3473543734481366E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.749764929470592E-5
72-73	0.011022043461855082
74-75	0.024026788746705437
76-77	0.0030426781355064156
78-79	0.0
80-81	0.0
82-83	0.005332376097253738
84-85	0.04300541770338013
86-87	0.044415199621438674
88-89	0.011604748203942925
90-91	1.2324242817777904E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	86.0
20-21	404.0
22-23	1013.0
24-25	2163.0
26-27	3876.0
28-29	7959.0
30-31	18957.0
32-33	24855.0
34-35	34754.0
36-37	50306.0
38-39	59652.0
40-41	64527.0
42-43	77236.0
44-45	91802.0
46-47	89254.0
48-49	77288.0
50-51	68492.0
52-53	67226.0
54-55	71647.0
56-57	73974.0
58-59	71846.0
60-61	71123.0
62-63	70925.0
64-65	74703.0
66-67	79299.0
68-69	77197.0
70-71	75188.0
72-73	73309.0
74-75	75584.0
76-77	75540.0
78-79	78213.0
80-81	78442.0
82-83	77005.0
84-85	78150.0
86-87	80833.0
88-89	85970.0
90-91	89624.0
92-93	91576.0
94-95	101827.0
96-97	265882.0
98-99	498751.0
100-101	5466555.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.36859027126707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.32093097652205	73.63191506958526
2	7.920804270253584	13.20692571651347
3	2.1243007985562548	5.312998886632855
4	0.7748080419884124	2.5837861676565854
5	0.35575764073443983	1.4829506493131073
6	0.18842069032383335	0.9425020398142174
7	0.10238604909911467	0.5975046403786546
8	0.060710187016707474	0.40490581653503066
9	0.0387849245679759	0.2910100036508624
>10	0.11097965281866862	1.355914636527134
>50	0.0016660983268825763	0.09065832442216935
>100	4.0549129842078506E-4	0.07034061329260757
>500	4.517849373142426E-5	0.028587435678102274
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1596874549533905E-5	0.0	0.0	0.0
3	0.0	1.1596874549533905E-5	0.0	1.1596874549533905E-5	0.0
4	0.0	3.479062364860171E-5	0.0	1.1596874549533905E-5	0.0
5	0.0	3.479062364860171E-5	0.0	1.1596874549533905E-5	0.0
6	0.0	3.479062364860171E-5	0.0	1.1596874549533905E-5	0.0
7	0.0	3.479062364860171E-5	0.0	2.319374909906781E-5	0.0
8	0.0	4.638749819813562E-5	0.0	3.479062364860171E-5	0.0
9	0.0	6.958124729720342E-5	0.0	4.638749819813562E-5	0.0
10-11	0.0	6.958124729720342E-5	0.0	5.218593547290257E-5	0.0
12-13	0.0	6.958124729720342E-5	0.0	6.378281002243648E-5	5.7984372747669525E-6
14-15	0.0	2.1454217916637723E-4	0.0	8.117812184673733E-5	2.319374909906781E-5
16-17	0.0	2.203406164411442E-4	0.0	8.117812184673733E-5	2.319374909906781E-5
18-19	0.0	2.6092967736451286E-4	0.0	9.277499639627124E-5	4.0589060923368666E-5
20-21	0.0	3.0151873828788154E-4	0.0	9.277499639627124E-5	8.117812184673733E-5
22-23	0.0	3.1891405011218236E-4	0.0	9.277499639627124E-5	8.117812184673733E-5
24-25	0.0	3.363093619364832E-4	0.0	9.277499639627124E-5	9.277499639627124E-5
26-27	0.0	3.479062364860171E-4	0.0	1.2756562004487295E-4	9.277499639627124E-5
28-29	0.0	3.479062364860171E-4	0.0	3.826968601346188E-4	9.277499639627124E-5
30-31	0.0	4.5227810743182225E-4	0.0	8.175796557421402E-4	9.277499639627124E-5
32-33	0.0	4.6387498198135617E-4	0.0	0.0021222280425647047	9.277499639627124E-5
34-35	0.0	5.334562292785596E-4	0.0	0.003873356099544324	9.277499639627124E-5
36-37	0.0	6.146343511252969E-4	0.0	0.006070963826680999	9.277499639627124E-5
38-39	0.0	6.262312256748308E-4	0.0	0.009538429316991635	1.2756562004487295E-4
40-41	0.0	7.190062220711021E-4	0.0	0.01320304167464435	1.5075936914394077E-4
42-43	0.0	7.537968457197038E-4	0.0	0.017018413401441005	1.5075936914394077E-4
44-45	0.0	8.987577775888776E-4	0.0	0.02127446636111995	1.5075936914394077E-4
46-47	0.0	0.0010089280858094497	0.0	0.026510455220234506	1.6235624369347466E-4
48-49	0.0	0.0010089280858094497	0.0	0.03156669252383129	1.681546809682416E-4
50-51	1.1596874549533905E-5	0.0011132999567552548	0.0	0.03640258921098692	1.8554999279254248E-4
52-53	1.1596874549533905E-5	0.0011248968313047887	0.0	0.041139912464471524	1.8554999279254248E-4
54-55	1.1596874549533905E-5	0.0011364937058543226	0.0	0.045593112291492544	1.8554999279254248E-4
56-57	1.1596874549533905E-5	0.0011480905804038565	0.0	0.05084069802515664	1.8554999279254248E-4
58-59	1.1596874549533905E-5	0.0011480905804038565	0.0	0.056360810310734775	1.9714686734207637E-4
60-61	1.1596874549533905E-5	0.0011538890176786235	0.0	0.061869325721763374	1.9714686734207637E-4
62-63	1.1596874549533905E-5	0.0011828812040524583	0.0	0.06694875677445923	1.9714686734207637E-4
64-65	1.1596874549533905E-5	0.0012988499495477973	0.0	0.07220214094539809	1.9714686734207637E-4
66-67	1.1596874549533905E-5	0.0013104468240973312	0.0	0.07742653292996311	1.9714686734207637E-4
68-69	1.1596874549533905E-5	0.0013510358850207	0.0	0.08303362177466275	2.029453046168433E-4
70-71	1.1596874549533905E-5	0.0013684311968450006	0.0	0.08782313096362025	2.0874374189161027E-4
72-73	1.1596874549533905E-5	0.0013742296341197676	0.0	0.09192842455415526	2.0874374189161027E-4
74-75	1.1596874549533905E-5	0.0013858265086693015	0.0	0.09519294473984904	2.0874374189161027E-4
76-77	1.1596874549533905E-5	0.0014670046305160387	0.0	0.0988343633484027	2.0874374189161027E-4
78-79	1.1596874549533905E-5	0.0015191905659889414	0.0	0.10110155232283657	2.0874374189161027E-4
80-81	1.1596874549533905E-5	0.0015191905659889414	0.0	0.10197711635132639	2.1454217916637723E-4
82-83	1.1596874549533905E-5	0.0015191905659889414	0.0	0.10246418508240682	2.203406164411442E-4
84-85	1.1596874549533905E-5	0.0015307874405384753	0.0	0.10263233976337505	2.203406164411442E-4
86-87	1.1596874549533905E-5	0.0015307874405384753	0.0	0.10271351788522179	2.2613905371591115E-4
88	1.1596874549533905E-5	0.0016351593114842804	0.0	0.10272511475977132	2.3193749099067809E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13825	0.0	50.778214	1
GTATCAA	25250	0.0	37.142044	1
ATCAACG	30805	0.0	30.047853	3
TATCAAC	31675	0.0	29.408676	2
CAACGCA	31515	0.0	29.320103	5
TCAACGC	31665	0.0	29.257692	4
AACGCAG	32360	0.0	28.722599	6
ACGCAGA	36700	0.0	25.199804	7
CGCAGAG	36985	0.0	24.994238	8
GTGGTAT	6620	0.0	22.646389	1
TGGTATC	6520	0.0	22.283247	2
GCAGAGT	42555	0.0	21.70297	9
GAGTACT	26220	0.0	20.365307	12-13
GTACATG	28695	0.0	19.92969	1
TACATGG	28170	0.0	19.688196	2
CAGAGTA	41620	0.0	19.353502	10-11
ACATGGG	28905	0.0	18.867039	3
AGAGTAC	39140	0.0	18.401886	10-11
GTACTTT	29860	0.0	17.826807	14-15
AGTACTT	28215	0.0	17.14246	12-13
>>END_MODULE