FastQCFastQC Report
Fri 27 May 2016
SRR2138620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8341956
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117750.049.5155141
GTATCAA213750.037.2084771
ATCAACG263350.029.6695793
TCAACGC263900.029.5749234
TATCAAC272050.029.0478442
CAACGCA268600.029.0459185
AACGCAG277700.028.1846086
ACGCAGA318850.024.507327
CGCAGAG319500.024.4309488
GTGGTAT60200.022.1012551
TGGTATC57550.021.4991512
GCAGAGT367100.021.1246669
GTACATG256450.020.1411721
GAGTACT217200.020.03585412-13
TACATGG254900.019.8813152
ACATGGG258450.019.0835953
CAGAGTA359400.018.7902410-11
AGAGTAC337150.018.4851510-11
CATGGGG198050.017.6289224
GTACTTT250150.017.2954914-15
Produced by FastQC (version 0.11.5)