##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138620_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8341956
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79743899392421	33.0	33.0	33.0	33.0	33.0
2	32.79457899322413	33.0	33.0	33.0	33.0	33.0
3	32.79445192470447	33.0	33.0	33.0	33.0	33.0
4	36.73389909992333	37.0	37.0	37.0	37.0	37.0
5	36.75389488987955	37.0	37.0	37.0	37.0	37.0
6	36.76563721985587	37.0	37.0	37.0	37.0	37.0
7	36.750862507546195	37.0	37.0	37.0	37.0	37.0
8	36.74705464761502	37.0	37.0	37.0	37.0	37.0
9	36.741712854874805	37.0	37.0	37.0	37.0	37.0
10-11	36.69702567359502	37.0	37.0	37.0	37.0	37.0
12-13	36.69790945912445	37.0	37.0	37.0	37.0	37.0
14-15	38.955989638401356	40.0	40.0	40.0	37.0	40.0
16-17	38.904291091921365	40.0	40.0	40.0	37.0	40.0
18-19	38.92984217370602	40.0	40.0	40.0	37.0	40.0
20-21	38.9729523264026	40.0	40.0	40.0	37.0	40.0
22-23	39.00659831690137	40.0	40.0	40.0	37.0	40.0
24-25	39.0154734226829	40.0	40.0	40.0	37.0	40.0
26-27	38.920560994332945	40.0	40.0	40.0	37.0	40.0
28-29	38.84871995077488	40.0	40.0	40.0	37.0	40.0
30-31	38.776532146550096	40.0	37.0	40.0	37.0	40.0
32-33	38.721711616004995	40.0	37.0	40.0	37.0	40.0
34-35	38.72136413744782	40.0	37.0	40.0	37.0	40.0
36-37	38.68524985193908	40.0	37.0	40.0	37.0	40.0
38-39	38.649859690894274	40.0	37.0	40.0	37.0	40.0
40-41	38.60251642442879	40.0	37.0	40.0	37.0	40.0
42-43	38.48028385144534	40.0	37.0	40.0	37.0	40.0
44-45	38.44245616707431	40.0	37.0	40.0	37.0	40.0
46-47	38.37588484440917	40.0	37.0	40.0	37.0	40.0
48-49	38.304268972803314	40.0	37.0	40.0	37.0	40.0
50-51	38.25998492095016	40.0	37.0	40.0	37.0	40.0
52-53	38.22090072285678	40.0	37.0	40.0	37.0	40.0
54-55	38.148009933455455	40.0	37.0	40.0	37.0	40.0
56-57	38.04803903469305	40.0	37.0	40.0	37.0	40.0
58-59	37.78601982195002	40.0	37.0	40.0	33.0	40.0
60-61	37.718528839164975	40.0	37.0	40.0	33.0	40.0
62-63	37.370620935388004	37.0	37.0	40.0	33.0	40.0
64-65	37.17931329922996	37.0	37.0	40.0	33.0	40.0
66-67	37.03693056578178	37.0	37.0	40.0	33.0	40.0
68-69	36.811136715425945	37.0	37.0	40.0	33.0	40.0
70-71	36.54450153312158	37.0	37.0	40.0	33.0	40.0
72-73	36.313796592094	37.0	37.0	37.0	33.0	40.0
74-75	36.06581893406212	37.0	37.0	37.0	33.0	40.0
76-77	34.25357705765041	35.0	33.0	37.0	30.0	37.0
78-79	35.63533026272121	37.0	35.0	37.0	33.0	37.0
80-81	35.77911112408924	37.0	37.0	37.0	33.0	37.0
82-83	35.723030332105104	37.0	37.0	37.0	33.0	37.0
84-85	35.28488934819431	37.0	33.0	37.0	33.0	37.0
86-87	35.38324505952012	37.0	33.0	37.0	33.0	37.0
88-89	35.39307194468511	37.0	33.0	37.0	33.0	37.0
90-91	35.33818531172075	37.0	33.0	37.0	33.0	37.0
92-93	35.26366095975111	37.0	33.0	37.0	33.0	37.0
94-95	35.21766199838252	37.0	33.0	37.0	33.0	37.0
96-97	35.175320784628035	37.0	33.0	37.0	33.0	37.0
98-99	35.13187911989952	37.0	33.0	37.0	33.0	37.0
100	35.06023017373985	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	20.0
21	118.0
22	420.0
23	1402.0
24	3339.0
25	7104.0
26	13144.0
27	21842.0
28	33737.0
29	49026.0
30	70516.0
31	97614.0
32	134223.0
33	188046.0
34	271896.0
35	422992.0
36	823249.0
37	2391981.0
38	3651657.0
39	159626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.374301183079844	20.534500541599595	14.797980233892385	23.293218041428172
2	14.599058062641424	20.91298491624746	41.85128763565763	22.636669385453484
3	18.09053695863497	26.075141818010938	30.658655816639303	25.175665406714792
4	12.588468931233074	18.177116416185818	39.03460116866947	30.199813483911637
5	13.448593830880887	37.65217145291715	35.40718473431569	13.492049981886279
6	29.68036513259001	37.86549581417116	18.828461813991826	13.625677239247006
7	26.36271397259827	32.98993665274667	23.038098019217554	17.609251355437504
8	24.433574092215302	36.511472848813874	21.111499509227812	17.943453549743012
9	25.54158760846976	17.203926752910228	21.605544311190325	35.64894132742968
10-11	24.248078028702142	26.950190099300453	28.618767588800516	20.182964283196892
12-13	25.035681080072823	24.513878999122028	28.88791309855866	21.562526822246486
14-15	22.197875414351262	25.877701824368287	26.85118454233036	25.073238218950088
16-17	21.506796487538416	29.0110736618606	27.519576943345182	21.9625529072558
18-19	21.486669313527905	28.121270359133998	29.438041869316976	20.954018458021118
20-21	22.44344989293956	27.153677627811845	29.35961476343758	21.043257715811016
22-23	22.182985059220695	27.091962959963606	29.159848433670128	21.56520354714557
24-25	21.906901988900117	27.233668708825636	29.267295952129267	21.592133350144977
26-27	21.844597109617258	27.434433132150332	29.13186522298723	21.589104535245184
28-29	21.725554806408294	27.547936515715698	29.161770282621507	21.564738395254498
30-31	22.002123093221257	27.388113035349765	29.044401177539765	21.565362693889213
32-33	21.878184601250187	27.451753149823166	28.966284799249486	21.703777449677162
34-35	21.941416330943998	27.52084216343539	29.001247239009782	21.53649426661083
36-37	21.96429993899335	27.501078302227715	28.87909511985669	21.655526638922243
38-39	21.824509314344112	27.59513004003996	28.93778003956332	21.642580606052604
40-41	22.09909008572896	27.444691017831758	28.7609538543257	21.69526504211358
42-43	22.011015367201445	27.58681927302199	28.533779595146303	21.868385764630265
44-45	22.103406274464675	27.70712527721802	28.345035885328496	21.84443256298881
46-47	22.165649425812166	27.69159386128099	28.035498411947458	22.107258300959387
48-49	22.18877142436348	27.847817982658	27.92648137238658	22.036929220591936
50-51	22.121460664004672	27.910958075826375	27.82781028036196	22.13977097980699
52-53	22.245652638978026	27.881287766916557	27.664633357327297	22.208426236778127
54-55	22.26549703178061	27.758048505971217	27.719847239514333	22.256607222733837
56-57	22.310789248533666	27.859144677917826	27.696733463277294	22.133332610271218
58-59	22.253025204168544	28.015784341167567	27.670285023592108	22.060905431071784
60-61	22.329746657441824	27.939775725389417	27.664619080103325	22.065858537065434
62-63	22.36895376269277	27.965875281366593	27.52295353225614	22.1422174236845
64-65	22.289937509789457	27.95956651958025	27.52430678999836	22.22618918063193
66-67	22.3634313266284	27.942093798612085	27.46014120280636	22.234333671953156
68-69	22.273374448510335	27.86145643031872	27.58048868525394	22.284680435917
70-71	22.25480824719081	27.709080311704092	27.63209424233546	22.404017198769633
72-73	22.34065745924693	27.73724704508802	27.58136690699166	22.340728588673393
74-75	22.22930436638241	27.912276287775068	27.493508313752724	22.364911032089797
76-77	22.337625041533126	27.852601032051105	27.395939582273865	22.4138343441419
78-79	22.29865456436273	27.83647016525606	27.387208436245075	22.477666834136134
80-81	22.285987951197885	27.86087327640286	27.467907472250758	22.385231300148504
82-83	22.344655994078675	27.811631894771583	27.56416050746015	22.279551603689594
84-85	22.50265119672242	27.830952691538396	27.375183892802273	22.291212218936913
86-87	22.25096823292933	27.879136279328243	27.508989018227293	22.360906469515136
88-89	22.335043851493598	27.79373839957544	27.458203009241483	22.41301473968948
90-91	22.349392896732272	27.88543782774775	27.4426525034845	22.322516772035478
92-93	22.416171810486794	27.865284385070478	27.443100091734575	22.27544371270816
94-95	22.26878300377355	27.92821373782499	27.481233434619433	22.321769823782027
96-97	22.327440225267797	28.045519621589992	27.55231651301956	22.074723640122652
98-99	23.42563632862711	29.940065781308945	27.738829027782103	18.895468862281838
100	22.344867743700668	30.935877904310523	21.639130348092642	25.08012400389617
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1936.0
1	1751.0
2	2335.5
3	3345.5
4	4572.5
5	7405.0
6	9768.0
7	11211.5
8	12273.5
9	12227.5
10	11414.0
11	10666.5
12	11703.0
13	15521.0
14	24300.5
15	36383.5
16	46568.5
17	53012.0
18	54691.5
19	52081.0
20	47302.0
21	42113.0
22	39315.0
23	41234.5
24	47706.5
25	58656.5
26	73297.0
27	89786.5
28	107011.0
29	126339.0
30	145369.0
31	162671.0
32	182348.5
33	201423.5
34	218670.0
35	235930.5
36	255147.5
37	273021.5
38	284735.0
39	293597.0
40	303480.5
41	313294.0
42	319821.0
43	325497.0
44	332756.0
45	340675.5
46	345765.5
47	347017.5
48	345393.5
49	340817.5
50	333130.5
51	324748.5
52	314397.0
53	298403.0
54	281154.0
55	261913.5
56	241524.0
57	219913.5
58	195412.0
59	169231.5
60	140643.5
61	113635.0
62	90412.0
63	69934.0
64	51542.5
65	37775.0
66	28468.5
67	21364.5
68	15974.0
69	11548.0
70	8070.5
71	5586.0
72	3782.5
73	2460.0
74	1576.5
75	1042.0
76	715.0
77	463.0
78	289.0
79	184.0
80	126.5
81	81.5
82	55.5
83	41.5
84	30.0
85	22.5
86	16.5
87	12.0
88	8.0
89	4.5
90	2.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0012347224080299633
4	0.03271415001469679
5	0.0025653455856156516
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.596351932526684E-5
22-23	0.0
24-25	0.0032255250546255857
26-27	0.03975640937910612
28-29	0.08647149033163526
30-31	0.08440980672750005
32-33	0.0376488946013473
34-35	0.002230076712221473
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.966771526183236E-5
56-57	7.936041326670674E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.1138400081002348E-5
72-73	0.010149139001668955
74-75	0.022512271916084358
76-77	0.0035370766815030603
78-79	0.0
80-81	0.0
82-83	0.004586604962138845
84-85	0.040657289833957076
86-87	0.042274858550762955
88-89	0.010136575516369876
90-91	1.0138980464680393E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	92.0
20-21	366.0
22-23	1035.0
24-25	2625.0
26-27	3958.0
28-29	8067.0
30-31	19649.0
32-33	25253.0
34-35	34183.0
36-37	49746.0
38-39	58610.0
40-41	60934.0
42-43	71562.0
44-45	83708.0
46-47	81725.0
48-49	71592.0
50-51	64055.0
52-53	62034.0
54-55	65463.0
56-57	67226.0
58-59	65966.0
60-61	64262.0
62-63	63003.0
64-65	66165.0
66-67	69328.0
68-69	68362.0
70-71	66872.0
72-73	64587.0
74-75	66653.0
76-77	66537.0
78-79	68754.0
80-81	69309.0
82-83	67678.0
84-85	66923.0
86-87	70868.0
88-89	74626.0
90-91	77060.0
92-93	80222.0
94-95	96508.0
96-97	265976.0
98-99	493810.0
100-101	5416604.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.58398217967165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.74381871762054	74.17561762249608
2	7.719340319214782	12.904264073601384
3	1.933434727336002	4.848125213856322
4	0.7421111126535141	2.481144080614705
5	0.3337236743313797	1.3946976824124295
6	0.17467697540570756	0.8760118319705763
7	0.10587109402967257	0.6194389345702946
8	0.0671576671572072	0.44906442039170613
9	0.04172276130687401	0.3138619083804406
>10	0.13540860668558302	1.6995281813672263
>50	0.002194228725653518	0.12265810069569043
>100	4.915789890677609E-4	0.07550599971616327
>500	2.4268274574312643E-5	0.015071301989561896
>1k	2.4268269434622364E-5	0.02501064793744448
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1987596194465662E-5	0.0	0.0	0.0
3	0.0	1.1987596194465662E-5	0.0	0.0	0.0
4	0.0	7.192557716679397E-5	0.0	0.0	0.0
5	1.1987596194465662E-5	7.192557716679397E-5	0.0	0.0	0.0
6	3.5962788583396986E-5	7.192557716679397E-5	0.0	0.0	0.0
7	5.993798097232831E-5	7.192557716679397E-5	0.0	0.0	0.0
8	5.993798097232831E-5	8.391317336125964E-5	0.0	0.0	1.1987596194465662E-5
9	5.993798097232831E-5	1.4385115433358794E-4	0.0	0.0	2.3975192388931324E-5
10-11	7.192557716679397E-5	1.4385115433358794E-4	0.0	0.0	2.3975192388931324E-5
12-13	7.192557716679397E-5	1.4385115433358794E-4	0.0	0.0	2.3975192388931324E-5
14-15	8.391317336125964E-5	2.097829334031491E-4	0.0	0.0	2.3975192388931324E-5
16-17	9.59007695557253E-5	2.1577673150038193E-4	0.0	1.1987596194465662E-5	3.5962788583396986E-5
18-19	1.0788836575019097E-4	2.2776432769484759E-4	0.0	1.1987596194465662E-5	3.5962788583396986E-5
20-21	1.0788836575019097E-4	2.3975192388931324E-4	0.0	1.1987596194465662E-5	3.5962788583396986E-5
22-23	1.3785735623635513E-4	2.757147124727102E-4	0.0	1.7981394291698493E-5	4.195658668062982E-5
24-25	2.0378913530591628E-4	3.1767129915334007E-4	0.0	4.795038477786265E-5	5.3944182875095476E-5
26-27	2.2177052959761477E-4	3.3565269344503856E-4	0.0	1.2586976004188945E-4	7.192557716679397E-5
28-29	2.3975192388931324E-4	3.3565269344503856E-4	0.0	4.854976458758593E-4	7.192557716679397E-5
30-31	2.3975192388931324E-4	4.195658668062982E-4	0.0	0.0016243192843500972	7.192557716679397E-5
32-33	2.3975192388931324E-4	4.3155346300076386E-4	0.0	0.0037221486183815883	9.59007695557253E-5
34-35	2.457457219865461E-4	4.61522453486928E-4	0.0	0.006611159301247813	1.0788836575019097E-4
36-37	2.517395200837789E-4	4.795038477786265E-4	0.0	0.010812811767408028	1.1388216384742379E-4
38-39	2.517395200837789E-4	5.274542325564892E-4	0.0	0.016686733902696205	1.1987596194465662E-4
40-41	2.7571471247271026E-4	5.754046173343518E-4	0.0	0.02381335984030604	1.1987596194465662E-4
42-43	3.056837029588744E-4	5.873922135288174E-4	0.0	0.029807157937538867	1.1987596194465662E-4
44-45	3.236650972505729E-4	7.012743773762413E-4	0.0	0.03681390791320405	1.1987596194465662E-4
46-47	3.656216839312027E-4	8.031689450291994E-4	0.0	0.04564876630852524	1.2586976004188945E-4
48-49	3.7161548202843556E-4	8.031689450291994E-4	0.0	0.0529252371985659	1.4385115433358794E-4
50-51	3.7161548202843556E-4	9.410263012655546E-4	0.0	0.059374563951188425	1.4385115433358794E-4
52-53	3.7161548202843556E-4	9.470200993627873E-4	0.0	0.06592578527146391	1.558387505280536E-4
54-55	3.7161548202843556E-4	9.530138974600201E-4	0.0	0.0720574407249331	1.6782634672251928E-4
56-57	3.8959687632013405E-4	9.829828879461843E-4	0.0	0.07827900314986078	1.6782634672251928E-4
58-59	4.0757827061183255E-4	9.829828879461843E-4	0.0	0.08480624927774733	1.6782634672251928E-4
60-61	4.0757827061183255E-4	0.0010069580803351158	0.0	0.09066818381684104	1.6782634672251928E-4
62-63	4.0757827061183255E-4	0.0010189456765295813	0.0	0.09636828580730947	1.6782634672251928E-4
64-65	4.0757827061183255E-4	0.001138821638474238	0.0	0.1019964622206111	1.6782634672251928E-4
66-67	4.0757827061183255E-4	0.001138821638474238	0.0	0.10808016728930242	1.6782634672251928E-4
68-69	4.0757827061183255E-4	0.0011568030327659363	0.0	0.11372033129879851	1.6782634672251928E-4
70-71	4.0757827061183255E-4	0.0011627968308631693	0.0	0.11867720232521006	1.6782634672251928E-4
72-73	4.0757827061183255E-4	0.0011627968308631693	0.0	0.12278295402181455	1.7981394291698494E-4
74-75	4.3155346300076386E-4	0.0012107472156410318	0.0	0.12571991508945862	1.7981394291698494E-4
76-77	4.435410591952295E-4	0.0012826727928078258	0.0	0.12847106841608852	1.7981394291698494E-4
78-79	4.435410591952295E-4	0.0013186355813912228	0.0	0.13061684813489785	1.7981394291698494E-4
80-81	4.5552865538969517E-4	0.0013186355813912228	0.0	0.13131212871417686	1.7981394291698494E-4
82-83	4.6751625158416085E-4	0.0013306231775856885	0.0	0.13180961395624718	1.7981394291698494E-4
84-85	4.6751625158416085E-4	0.001348604571877387	0.0	0.1320074092934559	1.7981394291698494E-4
86-87	4.6751625158416085E-4	0.0013785735623635513	0.0	0.13216924184208118	1.7981394291698494E-4
88	4.6751625158416085E-4	0.0013785735623635513	0.0	0.13218722323637286	1.7981394291698494E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11775	0.0	49.515514	1
GTATCAA	21375	0.0	37.208477	1
ATCAACG	26335	0.0	29.669579	3
TCAACGC	26390	0.0	29.574923	4
TATCAAC	27205	0.0	29.047844	2
CAACGCA	26860	0.0	29.045918	5
AACGCAG	27770	0.0	28.184608	6
ACGCAGA	31885	0.0	24.50732	7
CGCAGAG	31950	0.0	24.430948	8
GTGGTAT	6020	0.0	22.101255	1
TGGTATC	5755	0.0	21.499151	2
GCAGAGT	36710	0.0	21.124666	9
GTACATG	25645	0.0	20.141172	1
GAGTACT	21720	0.0	20.035854	12-13
TACATGG	25490	0.0	19.881315	2
ACATGGG	25845	0.0	19.083595	3
CAGAGTA	35940	0.0	18.79024	10-11
AGAGTAC	33715	0.0	18.48515	10-11
CATGGGG	19805	0.0	17.628922	4
GTACTTT	25015	0.0	17.29549	14-15
>>END_MODULE