FastQCFastQC Report
Fri 27 May 2016
SRR2138619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25376533
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA277650.042.7194251
GTATCAA473400.032.2012751
ATCAACG563100.026.3083573
TCAACGC563150.026.3060174
CAACGCA571200.025.913855
AACGCAG577000.025.7524366
TATCAAC615850.024.4012932
ACGCAGA667950.022.1603747
CGCAGAG676700.021.8375578
GTACATG631100.020.3091811
TACATGG637200.019.4991952
ACATGGG628500.019.3241423
CTTATAC427100.018.7404881
GCAGAGT802950.018.185869
TATACAC513900.017.5900763
GAGTACT478800.017.11557812-13
AGAGTAC740950.017.05548910-11
CATGGGG466800.016.6721824
CAGAGTA800850.016.3378310-11
TTATACA533300.015.9815462
Produced by FastQC (version 0.11.5)