##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138619_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25376533
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57903418091037	31.0	31.0	34.0	30.0	34.0
2	31.689429678987274	31.0	31.0	34.0	30.0	34.0
3	31.757057632734938	31.0	31.0	34.0	30.0	34.0
4	35.53373335120286	37.0	35.0	37.0	33.0	37.0
5	35.37115590218727	37.0	35.0	37.0	33.0	37.0
6	35.392873723136255	37.0	35.0	37.0	33.0	37.0
7	35.28940080191411	37.0	35.0	37.0	32.0	37.0
8	35.26820700841994	37.0	35.0	37.0	32.0	37.0
9	36.8523990649156	39.0	37.0	39.0	32.0	39.0
10-11	36.7184760030064	39.0	35.0	39.0	32.0	39.0
12-13	36.68307908728115	39.0	35.0	39.0	32.0	39.0
14-15	37.80837606933934	40.0	37.0	41.0	32.0	41.0
16-17	37.762825638947604	40.0	37.0	41.0	32.0	41.0
18-19	37.78713494865512	40.0	37.0	41.0	32.0	41.0
20-21	37.76487731558207	40.0	37.0	41.0	32.0	41.0
22-23	37.67293648714298	39.5	36.5	41.0	32.0	41.0
24-25	37.60588683987851	39.0	36.0	41.0	32.0	41.0
26-27	37.40786916525718	39.0	36.0	41.0	31.5	41.0
28-29	37.261413147447556	39.0	36.0	41.0	31.0	41.0
30-31	37.09997692352964	39.0	36.0	41.0	31.0	41.0
32-33	36.89430212191131	39.0	35.5	40.0	30.0	41.0
34-35	36.77436889788072	39.0	35.0	40.0	30.0	41.0
36-37	36.62481679242853	38.0	35.0	40.0	30.0	41.0
38-39	36.49148649991524	38.0	35.0	40.0	30.0	41.0
40-41	36.37443586624394	38.0	35.0	40.0	30.0	41.0
42-43	36.141106954197916	38.0	35.0	40.0	29.5	41.0
44-45	35.98565151490679	38.0	34.0	40.0	29.0	41.0
46-47	36.001787159784556	38.0	34.0	40.0	29.0	41.0
48-49	36.306477350550296	38.0	35.0	40.0	29.5	41.0
50-51	36.588712461295984	39.0	35.0	40.0	30.0	41.0
52-53	36.578092958469654	39.0	35.0	40.0	30.0	41.0
54-55	36.498787776590675	38.5	35.0	40.0	30.0	41.0
56-57	36.360715069675365	38.0	35.0	40.0	29.0	41.0
58-59	36.212083254803034	38.0	34.0	40.0	29.0	41.0
60-61	36.038254022328594	38.0	34.0	40.0	28.5	41.0
62-63	35.85281286762736	38.0	34.0	40.0	28.0	41.0
64-65	35.628086500921896	37.0	34.0	40.0	28.0	41.0
66-67	35.40503534001651	37.0	34.0	40.0	28.0	41.0
68-69	35.14492092592322	36.5	33.0	39.5	28.0	41.0
70-71	34.87629201154763	36.0	33.0	39.0	28.0	41.0
72-73	34.61033572430654	36.0	33.0	39.0	28.0	40.0
74-75	34.30079559130168	35.0	32.5	38.5	27.5	40.0
76-77	32.56499379979751	34.0	30.5	36.0	25.5	39.0
78-79	33.57205936985121	35.0	32.0	37.0	26.5	39.0
80-81	33.616768529016596	35.0	32.0	36.5	27.0	39.0
82-83	33.39993227279173	35.0	32.0	36.0	27.0	38.5
84-85	33.16152882878052	35.0	32.0	36.0	27.0	37.0
86-87	32.946210334515676	34.5	32.0	35.5	27.0	37.0
88-89	32.785298223461915	34.0	32.0	35.0	27.0	36.5
90-91	32.66575116373991	34.0	32.0	35.0	27.0	36.0
92-93	32.594834785029136	34.0	32.0	35.0	27.0	36.0
94-95	32.57103244633039	34.0	32.0	35.0	27.0	36.0
96-97	32.65181469110138	34.0	32.0	35.0	27.5	35.0
98-99	32.77672220552418	34.0	32.0	35.0	29.0	35.0
100	31.58444668548192	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	4.0
19	30.0
20	291.0
21	2081.0
22	9997.0
23	33010.0
24	81727.0
25	164189.0
26	279273.0
27	416238.0
28	564835.0
29	718676.0
30	872170.0
31	1037492.0
32	1234137.0
33	1481881.0
34	1824847.0
35	2302849.0
36	3074908.0
37	4181713.0
38	4579508.0
39	2423853.0
40	92822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.93285185962952	22.58810137696903	14.954765491408933	23.524281271992514
2	14.04535439325785	22.465142450329587	42.54185073763902	20.94765241877355
3	17.268028694069436	28.407229624314716	31.645414288862867	22.67932739275298
4	12.064508575698659	19.467828800727034	40.27097003361334	28.19669258996097
5	11.95443837816616	39.07305225658682	36.98681376214788	11.985695603099131
6	27.76761506388599	40.512866749764434	19.34405696790811	12.375461218441464
7	24.446173079594388	33.44115366692971	25.167813890621137	16.94485936285477
8	22.73739679096431	38.85175331082461	22.070579933042865	16.340269965168215
9	24.632639927605556	17.11439068528392	22.292379341181082	35.96059004592944
10-11	23.12468861469928	27.666735930172297	30.354484395594213	18.85409105953421
12-13	23.89368756101235	25.108664908676907	30.251633177871394	20.746014352439346
14-15	21.18545924676466	26.70678206256454	27.367576003617867	24.74018268705294
16-17	20.89638539175905	29.725157556792393	28.13146557002409	21.246991481424466
18-19	20.908671193923105	28.97478799001455	29.58342549531128	20.533115320751072
20-21	21.566410793922756	28.158285708182902	29.39158784117565	20.883715656718696
22-23	21.38277769069095	28.291971732785274	29.204762773972213	21.120487802551562
24-25	21.33877961758617	28.44645026234326	29.25349423336116	20.961275886709412
26-27	21.3725332578735	28.510910689280816	29.18591259040706	20.930643462438628
28-29	21.38017222330241	28.48608731142395	29.126441926718776	21.00729853855486
30-31	21.486480835925423	28.489829093258333	29.121210918849965	20.902479151966276
32-33	21.46350040316643	28.62683916464733	28.92964417771285	20.980016254473387
34-35	21.64005032377045	28.41532734664074	29.10201404689246	20.84260828269635
36-37	21.350465450844013	28.610059366978675	28.947972617688233	21.091502564489073
38-39	21.265546613488176	28.408854990701442	29.15854806553409	21.167050330276293
40-41	21.383024914984695	28.271464207749382	29.17613518072478	21.169375696541145
42-43	21.584931901199056	28.308141074729697	28.84215645637723	21.26477056769402
44-45	21.365005190479362	28.610863697303518	28.69136790449692	21.332763207720195
46-47	21.658130099118363	28.324138772645185	28.373303263257064	21.644427864979388
48-49	21.511810776458656	28.416430892635614	28.416390485775107	21.655367845130627
50-51	21.355586195746096	28.560066323852958	28.399283440945478	21.68506403945547
52-53	21.455285759656697	28.519305620517898	28.498208516366404	21.527200103459
54-55	21.516069162515905	28.46932019412518	28.328216353546765	21.68639428981215
56-57	21.676425018825576	28.540308742984124	28.12944344818502	21.65382279000528
58-59	21.432344838405136	28.6316272331751	28.41697972283952	21.519048205580248
60-61	21.670213069664836	28.85799099916499	28.071495903336523	21.400300027833644
62-63	21.474331201568468	28.969942586028907	28.065502053649677	21.490224158752945
64-65	21.420520255380787	28.851842491713388	28.038862619211987	21.68877463369384
66-67	21.54503719706936	28.77665910248076	27.90933441051291	21.768969289936972
68-69	21.49940648978273	28.736494505591732	27.980563616173537	21.78353538845201
70-71	21.58846250096481	28.380333229588295	28.139234913698697	21.891969355748202
72-73	21.655585472384388	28.401142843175382	28.06211185431765	21.88115983012258
74-75	21.54697846996108	28.596595539136516	28.005990146678585	21.85043584422382
76-77	21.681297374281407	28.522181092253206	28.002562363125755	21.79395917033963
78-79	21.667525504522203	28.430099311658264	27.949811823048083	21.952563360771453
80-81	21.602096855720376	28.556475638857986	27.959955610269493	21.881471895152142
82-83	21.764703036284946	28.453808735471277	28.049857766961455	21.731630461282318
84-85	21.86573679596164	28.4765941994737	27.90404295465571	21.75362604990895
86-87	21.625399479321977	28.620725036872713	28.040137806476316	21.71373767732899
88-89	21.752413082026	28.519013828501727	27.98259670680208	21.745976382670197
90-91	21.826962008728355	28.482562181939507	28.06306152831606	21.627414281016083
92-93	21.833870557000065	28.511123460535497	28.042189397670818	21.612816584793617
94-95	21.553134942692726	28.54745965475058	28.16204785527815	21.73735754727855
96-97	21.57976386754914	28.64860805689362	28.290575479966133	21.481052595591102
98-99	22.556715496796965	30.664128967022812	28.539026307422617	18.240129228757606
100	21.919118791870883	31.284635945701844	22.255680140147398	24.540565122279876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5137.0
1	4701.5
2	6614.0
3	9383.5
4	12366.0
5	20611.5
6	28112.5
7	32642.0
8	34680.5
9	32980.0
10	29549.5
11	25817.0
12	26685.5
13	34022.5
14	52932.5
15	80442.5
16	103833.0
17	118588.5
18	124972.5
19	125185.0
20	122099.0
21	118899.5
22	120359.0
23	133729.0
24	161920.0
25	206038.5
26	266782.5
27	339170.0
28	426674.0
29	532952.0
30	639211.5
31	740870.5
32	844207.5
33	935629.5
34	1014981.0
35	1074518.5
36	1120074.0
37	1153191.5
38	1144416.5
39	1108038.0
40	1074344.0
41	1036387.0
42	1000526.5
43	979990.0
44	962481.0
45	956116.0
46	961696.0
47	966648.5
48	947893.5
49	915700.5
50	893589.5
51	866789.0
52	824107.5
53	769187.0
54	714768.0
55	656695.5
56	600767.5
57	544605.5
58	483256.5
59	424245.0
60	361280.0
61	297109.5
62	240496.0
63	190604.0
64	146904.0
65	112821.0
66	86452.5
67	66270.5
68	50785.0
69	38016.5
70	28541.0
71	21693.5
72	16311.5
73	12206.0
74	9144.0
75	6710.5
76	4769.0
77	3366.0
78	2395.0
79	1666.0
80	1160.0
81	829.0
82	628.0
83	435.0
84	293.5
85	223.0
86	169.5
87	124.0
88	84.0
89	52.5
90	35.5
91	28.5
92	22.0
93	16.0
94	10.5
95	6.5
96	5.0
97	4.5
98	2.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.11149671233655126
3	0.0
4	0.0
5	0.0
6	0.0
7	2.2855762053862914E-4
8	0.0
9	0.0
10-11	5.5169080819669106E-5
12-13	4.137681061475182E-5
14-15	9.496963198243037E-4
16-17	1.9112145855385368E-4
18-19	6.305037807962182E-5
20-21	7.448301692632471E-4
22-23	4.0795096027418875E-4
24-25	3.114886476188073E-4
26-27	0.002000211107093374
28-29	0.0038695754080743665
30-31	0.01072930158021747
32-33	0.008095024272966997
34-35	0.0071259694038453055
36-37	0.0037312509399516076
38-39	5.653015905184226E-4
40-41	2.0768297934346404E-4
42-43	3.227153010547323E-4
44-45	3.323700487638794E-5
46-47	0.0
48-49	2.1266764164855874E-5
50-51	0.0
52-53	0.0013162438830497762
54-55	6.889488523055586E-4
56-57	6.834320537621381E-4
58-59	4.718364952632222E-5
60-61	4.940185621214113E-4
62-63	3.57584490467132E-4
64-65	9.051661410232007E-4
66-67	8.925773646178535E-4
68-69	3.109834884160579E-4
70-71	2.618499010293026E-4
72-73	2.48405281563288E-6
74-75	1.7645358937858292E-5
76-77	3.147039108254998E-4
78-79	1.1692514615838144E-4
80-81	3.2496423610062577E-4
82-83	5.64469833401567E-5
84-85	1.3667706669802585E-5
86-87	0.0
88-89	5.668603250490476E-6
90-91	0.0
92-93	0.0
94-95	1.1866862204156463E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	942.0
20-21	3396.0
22-23	7046.0
24-25	13603.0
26-27	19120.0
28-29	32077.0
30-31	83266.0
32-33	96716.0
34-35	128571.0
36-37	176924.0
38-39	203961.0
40-41	226192.0
42-43	247295.0
44-45	279397.0
46-47	280893.0
48-49	259136.0
50-51	240739.0
52-53	229442.0
54-55	246474.0
56-57	277240.0
58-59	288021.0
60-61	291778.0
62-63	293412.0
64-65	302789.0
66-67	329131.0
68-69	310627.0
70-71	306506.0
72-73	293633.0
74-75	296559.0
76-77	289870.0
78-79	316118.0
80-81	325404.0
82-83	311531.0
84-85	311862.0
86-87	327719.0
88-89	359726.0
90-91	392363.0
92-93	425676.0
94-95	510772.0
96-97	1046720.0
98-99	1602271.0
100-101	1.3391615E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.95978501076428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.18968372565598	71.39817834596406
2	7.448073554361673	12.059888674109597
3	2.147857140611268	5.216701570131694
4	0.9190347503209616	2.976194232136259
5	0.4562311265599882	1.8468186960757713
6	0.26596229663973553	1.291935021415326
7	0.15857608701779138	0.8986800138966047
8	0.10171498550789909	0.6587858687274011
9	0.07470368221117714	0.5443194646196421
>10	0.23442134354134259	2.8305673038261157
>50	0.0029564833249266604	0.1581129422875169
>100	7.55368864869275E-4	0.09767063830429298
>500	1.9651449619850674E-5	0.012416168769280556
>1k	9.803932718987419E-6	0.009731059736419826
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.940648629976364E-6	0.0
2	0.0	7.881297259952728E-6	0.0	3.940648629976364E-6	0.0
3	0.0	7.881297259952728E-6	0.0	7.881297259952728E-6	0.0
4	0.0	3.940648629976364E-5	0.0	2.3643891779858187E-5	0.0
5	3.940648629976364E-6	4.3347134929740006E-5	0.0	3.152518903981091E-5	0.0
6	3.940648629976364E-6	4.3347134929740006E-5	0.0	6.699102670959819E-5	0.0
7	3.940648629976364E-6	4.7287783559716375E-5	0.0	7.093167533957456E-5	0.0
8	1.5762594519905456E-5	4.7287783559716375E-5	0.0	7.487232396955092E-5	0.0
9	1.5762594519905456E-5	1.3398205341919639E-4	0.0	1.0245686437938547E-4	0.0
10-11	2.955486472482273E-5	1.3595237773418456E-4	0.0	1.4974464793910184E-4	0.0
12-13	3.152518903981091E-5	1.3792270204917274E-4	0.0	1.970324314988182E-4	0.0
14-15	4.137681061475183E-5	1.990027558138064E-4	0.0	2.009730801287946E-4	0.0
16-17	4.7287783559716375E-5	2.0294340444378277E-4	0.0	2.502311880034991E-4	7.881297259952728E-6
18-19	6.896135102458637E-5	2.2264664759366458E-4	0.0	3.1919253902808556E-4	7.881297259952728E-6
20-21	9.85162157494091E-5	2.423498907435464E-4	0.0	4.039164845725773E-4	7.881297259952728E-6
22-23	1.2018978321427911E-4	2.7584540409834554E-4	0.0	4.8272945717210464E-4	7.881297259952728E-6
24-25	1.4777432362411366E-4	3.2707383628803827E-4	0.0	5.418391866217501E-4	7.881297259952728E-6
26-27	1.7141821540397184E-4	3.5859902532784917E-4	0.0	7.13257402025722E-4	7.881297259952728E-6
28-29	1.8521048560888912E-4	3.5859902532784917E-4	0.0	0.0016314285328102149	7.881297259952728E-6
30-31	1.8521048560888912E-4	4.1376810614751825E-4	0.0	0.005355341488137879	7.881297259952728E-6
32-33	2.1870599896368822E-4	4.236197277224592E-4	0.0	0.01502372290178489	1.1821945889929094E-5
34-35	2.522015123184873E-4	4.886404301170692E-4	0.0	0.026433871009881454	1.970324314988182E-5
36-37	2.522015123184873E-4	5.51690808196691E-4	0.0	0.04268510595990398	1.970324314988182E-5
38-39	2.522015123184873E-4	5.713940513465729E-4	0.0	0.08531504283898829	2.3643891779858187E-5
40-41	2.758454040983455E-4	6.265631321662419E-4	0.0	0.12765928269239932	2.3643891779858187E-5
42-43	2.876673499882746E-4	6.502070239461002E-4	0.0	0.1572712868223567	2.561421609484637E-5
44-45	2.9160799861825096E-4	7.349309694905919E-4	0.0	0.19729251430839667	2.758454040983455E-5
46-47	3.113112417681328E-4	7.920703746252492E-4	0.0	0.2328312539778385	2.758454040983455E-5
48-49	3.113112417681328E-4	7.920703746252492E-4	0.0	0.26675826835762	2.758454040983455E-5
50-51	3.1919253902808556E-4	8.886162660596701E-4	0.0	0.30076803635863103	2.758454040983455E-5
52-53	3.34955133547991E-4	8.905865903746583E-4	0.0	0.37928940095954006	3.3495513354799096E-5
54-55	3.428364308079437E-4	8.905865903746583E-4	0.0	0.47432208332005005	3.940648629976364E-5
56-57	3.428364308079437E-4	8.945272390046348E-4	0.0	0.5688286890884582	3.940648629976364E-5
58-59	3.6056934964283734E-4	8.945272390046348E-4	0.0	0.6219446919719096	3.940648629976364E-5
60-61	3.78302268477731E-4	9.024085362645875E-4	0.0	0.6579937456389333	3.940648629976364E-5
62-63	4.039164845725773E-4	9.162008064695047E-4	0.0	0.6862442556672339	4.3347134929740006E-5
64-65	4.177087547774946E-4	0.0010127466979039258	0.0	0.6942102768727312	4.3347134929740006E-5
66-67	4.2559005203744737E-4	0.0010186576708488904	0.0	0.7015182097570223	4.3347134929740006E-5
68-69	4.492339438173055E-4	0.0010521531842036893	0.0	0.7085246830211203	4.3347134929740006E-5
70-71	4.551449167622701E-4	0.001060034481463642	0.0	0.7149617325581867	5.51690808196691E-5
72-73	4.5908556539224645E-4	0.00106200480577863	0.0	0.7203485992353644	5.51690808196691E-5
74-75	4.610558897072346E-4	0.0010895893461884648	0.0	0.7246242029988887	5.51690808196691E-5
76-77	4.630262140222228E-4	0.0011191442109132876	0.0	0.728421017953871	5.51690808196691E-5
78-79	4.6696686265219916E-4	0.0011309661568032166	0.0	0.7308977156178111	5.51690808196691E-5
80-81	4.965217273770219E-4	0.001132936481118205	0.0	0.7318237680458556	5.51690808196691E-5
82-83	4.965217273770219E-4	0.0011388474540631693	0.0	0.7325803725828111	5.51690808196691E-5
84-85	4.965217273770219E-4	0.001146728751323122	0.0	0.7328995651218391	5.51690808196691E-5
86-87	4.965217273770219E-4	0.0011624913458430275	0.0	0.7330591613913532	5.51690808196691E-5
88	5.044030246369746E-4	0.0012255417239226494	0.0	0.7330709833372431	5.51690808196691E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	27765	0.0	42.719425	1
GTATCAA	47340	0.0	32.201275	1
ATCAACG	56310	0.0	26.308357	3
TCAACGC	56315	0.0	26.306017	4
CAACGCA	57120	0.0	25.91385	5
AACGCAG	57700	0.0	25.752436	6
TATCAAC	61585	0.0	24.401293	2
ACGCAGA	66795	0.0	22.160374	7
CGCAGAG	67670	0.0	21.837557	8
GTACATG	63110	0.0	20.309181	1
TACATGG	63720	0.0	19.499195	2
ACATGGG	62850	0.0	19.324142	3
CTTATAC	42710	0.0	18.740488	1
GCAGAGT	80295	0.0	18.18586	9
TATACAC	51390	0.0	17.590076	3
GAGTACT	47880	0.0	17.115578	12-13
AGAGTAC	74095	0.0	17.055489	10-11
CATGGGG	46680	0.0	16.672182	4
CAGAGTA	80085	0.0	16.33783	10-11
TTATACA	53330	0.0	15.981546	2
>>END_MODULE