FastQCFastQC Report
Fri 27 May 2016
SRR2138618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24441607
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA300400.042.34611
GTATCAA518300.030.899841
TCAACGC582400.026.654664
ATCAACG584800.026.5523153
CAACGCA590700.026.2661865
AACGCAG602300.025.8013446
TATCAAC634650.024.7541982
CGCAGAG685150.022.5911648
ACGCAGA686200.022.5626457
CTTATAC399200.021.7326851
TATACAC525800.019.0115533
GCAGAGT812800.018.8609569
GTACATG594900.018.8115251
TACATGG588050.018.5257912
ACATGGG585950.018.1635323
GAGTACT502300.017.70368212-13
TTATACA520900.017.5496922
AGAGTAC745450.017.4488710-11
CAGAGTA805750.017.19352110-11
TATGCCG246400.017.11203442-43
Produced by FastQC (version 0.11.5)