##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138618_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24441607
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.584414396320177	31.0	31.0	34.0	30.0	34.0
2	31.702527333820562	31.0	31.0	34.0	30.0	34.0
3	31.76776768401521	31.0	31.0	34.0	30.0	34.0
4	35.53978435215	37.0	35.0	37.0	33.0	37.0
5	35.382967412903746	37.0	35.0	37.0	33.0	37.0
6	35.40403439921115	37.0	35.0	37.0	33.0	37.0
7	35.30383435917287	37.0	35.0	37.0	32.0	37.0
8	35.2823687493216	37.0	35.0	37.0	32.0	37.0
9	36.865099172898084	39.0	37.0	39.0	33.0	39.0
10-11	36.735834595491205	39.0	35.0	39.0	32.0	39.0
12-13	36.70223760246206	39.0	35.0	39.0	32.0	39.0
14-15	37.83045141017119	40.0	37.0	41.0	32.0	41.0
16-17	37.78531403438407	40.0	37.0	41.0	32.0	41.0
18-19	37.815636058627405	40.0	37.0	41.0	32.0	41.0
20-21	37.79813420735791	40.0	37.0	41.0	32.0	41.0
22-23	37.70687263151042	40.0	37.0	41.0	32.0	41.0
24-25	37.64199840246897	39.0	36.5	41.0	32.0	41.0
26-27	37.45067939327967	39.0	36.0	41.0	32.0	41.0
28-29	37.303273470487966	39.0	36.0	41.0	31.0	41.0
30-31	37.15476160819941	39.0	36.0	41.0	31.0	41.0
32-33	36.941039334684646	39.0	36.0	40.0	30.0	41.0
34-35	36.81754947709748	39.0	35.0	40.0	30.0	41.0
36-37	36.67443542352551	38.5	35.0	40.0	30.0	41.0
38-39	36.53025219826439	38.0	35.0	40.0	30.0	41.0
40-41	36.42396209139426	38.0	35.0	40.0	30.0	41.0
42-43	36.20408618526862	38.0	35.0	40.0	29.5	41.0
44-45	36.055551070798316	38.0	34.5	40.0	29.0	41.0
46-47	36.0722282879507	38.0	34.5	40.0	29.0	41.0
48-49	36.37777724030936	38.5	35.0	40.0	30.0	41.0
50-51	36.66142101325424	39.0	35.0	40.0	30.0	41.0
52-53	36.65971353562351	39.0	35.0	40.0	30.0	41.0
54-55	36.58731821306541	39.0	35.0	40.0	30.0	41.0
56-57	36.46109079658629	38.5	35.0	40.0	29.5	41.0
58-59	36.32356577054672	38.0	34.5	40.0	29.0	41.0
60-61	36.158906080447906	38.0	34.0	40.0	29.0	41.0
62-63	35.98380519348213	38.0	34.0	40.0	28.5	41.0
64-65	35.77042494638338	38.0	34.0	40.0	28.0	41.0
66-67	35.55319879515538	37.0	34.0	40.0	28.0	41.0
68-69	35.30098501584896	37.0	34.0	39.5	28.0	41.0
70-71	35.02987091657619	36.0	33.0	39.0	28.0	41.0
72-73	34.75989186725378	36.0	33.0	39.0	28.0	40.5
74-75	34.450708365210616	35.5	33.0	39.0	28.0	40.0
76-77	32.70488212624619	34.0	30.5	36.5	26.0	39.0
78-79	33.70934612785132	35.0	32.0	37.0	27.0	39.0
80-81	33.741224292598986	35.0	32.0	37.0	28.0	39.0
82-83	33.5152228457527	35.0	32.0	36.0	27.5	39.0
84-85	33.2632406195677	35.0	32.0	36.0	27.0	37.0
86-87	33.03627749891555	35.0	32.0	35.5	27.0	37.0
88-89	32.86344032244121	34.0	32.0	35.0	27.0	37.0
90-91	32.736294072693255	34.0	32.0	35.0	27.0	36.0
92-93	32.65505681302324	34.0	32.0	35.0	27.0	36.0
94-95	32.620073284965514	34.0	32.0	35.0	27.0	36.0
96-97	32.69371745379101	34.0	32.0	35.0	28.0	35.5
98-99	32.817334291124126	34.0	32.0	35.0	29.0	35.0
100	31.6278347521929	34.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	5.0
19	31.0
20	276.0
21	1945.0
22	9313.0
23	30775.0
24	77075.0
25	154294.0
26	262445.0
27	391838.0
28	533838.0
29	681034.0
30	830660.0
31	989238.0
32	1174470.0
33	1407693.0
34	1733262.0
35	2182162.0
36	2913551.0
37	4031066.0
38	4560551.0
39	2383789.0
40	92294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.758290320272316	22.802093168423827	15.216564933721422	23.22305157758244
2	13.877345034091713	22.749906775351207	42.52075354351969	20.85199464703739
3	17.032734386081895	28.507638634399118	32.13571431698415	22.323912662534834
4	11.798214560786823	19.99133853934715	40.58122615106496	27.62922074880107
5	11.718390693377895	39.238999301478	37.30568943359575	11.736920571548344
6	27.25238974671346	40.98829917361817	19.539570372766406	12.219740706901964
7	24.035531118491164	33.83852610723186	25.17168195599291	16.95426081828407
8	22.54584978802744	38.971377782156466	22.216423821887	16.266348607929093
9	24.322713314226842	17.223838841693183	22.356602820755608	36.09684502332436
10-11	22.729504950106165	27.90274710772786	30.6872255524056	18.680522389760373
12-13	23.68376763369798	25.32639600520269	30.51240466531832	20.47743169578101
14-15	20.89346604938499	26.949581025666582	27.668098911377825	24.488854013570606
16-17	20.536512654855557	30.195827869792474	28.36102734329595	20.906632132056018
18-19	20.5445010699285	29.29786288627377	29.90639335134812	20.25124269244961
20-21	21.229685469577543	28.433863447468422	29.763495492238384	20.572955590715647
22-23	20.9759952244051	28.688423841495396	29.55711877870225	20.77846215539725
24-25	20.94519602241978	28.884403273723997	29.542444737772456	20.62795596608377
26-27	20.967543733602	29.05215066245755	29.39295628778002	20.58734931616043
28-29	20.871064661690507	28.992259439656486	29.446681435384942	20.689994463268057
30-31	21.286996951599228	28.771067580560572	29.39012414418962	20.55181132365058
32-33	20.999730176468052	28.86411585577259	29.412674635957625	20.723479331801737
34-35	21.051419066575498	28.75173237977239	29.4617495840386	20.735098969613517
36-37	20.9133844019455	28.98775987788505	29.392802119394457	20.706053600774986
38-39	20.867141579936032	28.694736285470984	29.558662762571352	20.879459372021625
40-41	21.01655624458149	28.607559255210578	29.542559079033182	20.83332542117475
42-43	21.206660603800735	28.67749846773314	29.235564394343598	20.880276534122526
44-45	20.997162686785632	28.930863882409742	29.080890946621324	20.991082484183305
46-47	21.29912328469868	28.67651898266975	28.737956348725323	21.28640138390624
48-49	21.145331246404908	28.795559311243284	28.73699604906232	21.32211339328948
50-51	20.970146929513927	28.82595196157491	28.90344030996405	21.300460798947114
52-53	21.07063222177441	28.85763310064743	28.923495929695537	21.148238747882626
54-55	21.11026726624945	28.792092188645608	28.77565541412318	21.321985130981762
56-57	21.319523163367457	28.85602330234926	28.54791309172045	21.27654044256283
58-59	20.991897740902406	29.055399300248208	28.845532902058984	21.10717005679041
60-61	21.28208121071091	29.295177061503303	28.437558517283158	20.98518321050263
62-63	21.058450531706995	29.431782216868825	28.4000126454538	21.109754605970384
64-65	21.039030390121084	29.306926569373093	28.413445239201877	21.240597801303945
66-67	21.14570485630132	29.222129953237086	28.315813676304348	21.316351514157244
68-69	21.146774203184513	29.097516926924083	28.39906791502274	21.35664095486866
70-71	21.192708709831994	28.845953218016028	28.497582866082848	21.463755206069127
72-73	21.245510771306936	28.85800893420453	28.421504268167418	21.47497602632111
74-75	21.23045644807654	28.981889961228596	28.365503095925465	21.422150494769397
76-77	21.30619373130678	28.936536853492612	28.309028725665737	21.448240689534874
78-79	21.264914266420682	28.93088303838745	28.280546265013434	21.523656430178438
80-81	21.269607651760253	28.945321408976177	28.307276435890504	21.477794503373065
82-83	21.312541548426132	28.944197377835696	28.39833187075599	21.344929202982186
84-85	21.40421526649013	28.954683304163904	28.324753282257113	21.316348147088853
86-87	21.241540793108665	29.015049012591156	28.406872331445886	21.336537862854296
88-89	21.312306207227714	28.97291020260793	28.380371405643196	21.334412184521163
90-91	21.352303324477052	29.015226463805423	28.394775999705757	21.23769421201177
92-93	21.376034498727574	28.962781402527565	28.385450499727717	21.275733599017148
94-95	21.195147723977932	28.969653825860725	28.54404714499235	21.29115130516899
96-97	21.12042949872545	29.07824304352602	28.737919459914274	21.063407997834254
98-99	22.122406168181357	31.013412289290223	29.032867467348378	17.831314075180046
100	21.44244867842859	31.71682182452813	22.812605451304975	24.028124045738302
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5266.0
1	4674.0
2	6590.0
3	9506.5
4	12598.5
5	20848.5
6	28179.5
7	32537.0
8	34545.5
9	32853.5
10	29399.0
11	25987.5
12	27548.5
13	35824.5
14	56445.0
15	86067.5
16	111542.0
17	127496.5
18	135045.5
19	136708.0
20	133440.0
21	129957.0
22	131502.0
23	145708.5
24	175951.0
25	222144.5
26	284305.0
27	357038.0
28	442098.5
29	539950.5
30	640436.5
31	738854.0
32	831897.0
33	908852.5
34	974370.5
35	1023814.5
36	1059354.0
37	1083637.0
38	1080201.5
39	1055765.5
40	1033735.5
41	1013093.5
42	985656.5
43	962687.5
44	942467.5
45	933558.5
46	944027.0
47	950277.5
48	925813.5
49	888834.5
50	859978.5
51	821802.5
52	766305.5
53	698961.5
54	630866.5
55	570683.0
56	514764.0
57	457909.5
58	400814.5
59	345055.5
60	288243.5
61	233214.0
62	184861.0
63	143886.0
64	110320.0
65	84855.0
66	66214.5
67	52013.5
68	40020.0
69	30079.5
70	22750.5
71	17060.5
72	12718.5
73	9685.5
74	7396.5
75	5551.0
76	4172.0
77	2976.5
78	2028.5
79	1443.0
80	1035.0
81	715.0
82	529.5
83	408.5
84	305.5
85	218.5
86	153.5
87	119.0
88	95.5
89	69.5
90	42.0
91	25.0
92	21.5
93	15.5
94	8.5
95	4.5
96	3.0
97	2.5
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.11236167900089385
3	0.0
4	8.182768015212747E-6
5	0.0
6	0.0
7	1.882036643498932E-4
8	0.0
9	0.0
10-11	4.091384007606374E-5
12-13	6.750783612550517E-5
14-15	0.0010351201539244128
16-17	1.6365536030425496E-4
18-19	8.387337215593065E-5
20-21	7.426308161060544E-4
22-23	4.194628984247879E-4
24-25	3.704794370300911E-4
26-27	0.0021483961751502818
28-29	0.003952016776885162
30-31	0.010983115077543995
32-33	0.008122062717196428
34-35	0.007311981575129867
36-37	0.0038542484555409012
38-39	6.343078395173533E-4
40-41	2.278696479274686E-4
42-43	3.214275025376808E-4
44-45	2.3650004528975867E-5
46-47	0.0
48-49	4.395814331967986E-5
50-51	0.0
52-53	0.001366569498348019
54-55	6.76005826582394E-4
56-57	8.633470530904537E-4
58-59	7.161487902144691E-5
60-61	5.028061494267846E-4
62-63	4.049370493388089E-4
64-65	9.140697210158448E-4
66-67	9.076220346491647E-4
68-69	3.2587515377233817E-4
70-71	2.7533344256561914E-4
72-73	0.0
74-75	2.5719006327672844E-6
76-77	2.8941733016893287E-4
78-79	9.784302408987807E-5
80-81	3.168371787700617E-4
82-83	3.2734079630340586E-5
84-85	8.312615063910432E-6
86-87	0.0
88-89	1.1465754542961264E-5
90-91	0.0
92-93	0.0
94-95	1.0115309625336289E-4
96-97	0.0
98-99	7.021552303718649E-6
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	845.0
20-21	3330.0
22-23	6835.0
24-25	12919.0
26-27	18589.0
28-29	31134.0
30-31	74298.0
32-33	87634.0
34-35	118329.0
36-37	159979.0
38-39	181142.0
40-41	203095.0
42-43	225968.0
44-45	254515.0
46-47	255114.0
48-49	229448.0
50-51	209722.0
52-53	201737.0
54-55	216328.0
56-57	243923.0
58-59	258803.0
60-61	268199.0
62-63	270898.0
64-65	281250.0
66-67	303741.0
68-69	279770.0
70-71	273045.0
72-73	264223.0
74-75	268041.0
76-77	260193.0
78-79	284402.0
80-81	292917.0
82-83	285290.0
84-85	286286.0
86-87	304769.0
88-89	333680.0
90-91	364204.0
92-93	401754.0
94-95	485747.0
96-97	1017620.0
98-99	1580425.0
100-101	1.3341466E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.90953129990802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.7958695822669	70.157267883868
2	7.578983701053008	12.11266070561576
3	2.2340890397137674	5.355750241373663
4	0.9548993870208284	3.0522224982161545
5	0.4817385861687255	1.9247752314911604
6	0.29769796346967886	1.4273342837879506
7	0.17748877422057419	0.9928131331272896
8	0.11590625138796053	0.7409611378512991
9	0.08344190595578156	0.6001023236127757
>10	0.27541514265070544	3.323047141514715
>50	0.003673342005712951	0.1935929430542606
>100	7.302408226861256E-4	0.08272584627220814
>500	6.60832637644234E-5	0.03674663021464786
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.045692003803187E-5	0.0	8.182768015212747E-6	0.0
3	0.0	2.045692003803187E-5	0.0	1.6365536030425494E-5	0.0
4	0.0	4.091384007606374E-5	0.0	3.273107206085099E-5	0.0
5	1.6365536030425494E-5	4.091384007606374E-5	0.0	3.273107206085099E-5	0.0
6	7.77362961445211E-5	4.091384007606374E-5	0.0	3.273107206085099E-5	0.0
7	7.77362961445211E-5	4.091384007606374E-5	0.0	6.137076011409561E-5	4.0913840076063735E-6
8	8.591906415973386E-5	6.137076011409561E-5	0.0	6.137076011409561E-5	4.0913840076063735E-6
9	8.591906415973386E-5	1.268329042357976E-4	0.0	8.591906415973386E-5	4.0913840076063735E-6
10-11	1.2069582822438803E-4	1.268329042357976E-4	0.0	1.0433029219396253E-4	4.0913840076063735E-6
12-13	1.3501567225101033E-4	1.288785962396008E-4	0.0	1.3092428824340395E-4	4.0913840076063735E-6
14-15	1.431984402662231E-4	1.9638643236510596E-4	0.0	1.7797520433087725E-4	4.0913840076063735E-6
16-17	1.8206658833848363E-4	2.004778163727123E-4	0.0	2.045692003803187E-4	4.0913840076063735E-6
18-19	1.882036643498932E-4	2.18889044406941E-4	0.0	2.2707181242215374E-4	4.0913840076063735E-6
20-21	1.882036643498932E-4	2.332088884335633E-4	0.0	2.598028844830047E-4	4.0913840076063735E-6
22-23	1.902493563536964E-4	2.782141125172334E-4	0.0	2.904882645400525E-4	4.0913840076063735E-6
24-25	2.229804284145474E-4	3.2321933660090353E-4	0.0	3.886814807226055E-4	4.0913840076063735E-6
26-27	2.4343734845257926E-4	3.457219486427386E-4	0.0	5.912049890991211E-4	4.0913840076063735E-6
28-29	2.495744244639888E-4	3.4776764064654175E-4	0.0	0.0015281319268409805	4.0913840076063735E-6
30-31	2.536658084715952E-4	3.804987127073928E-4	0.0	0.005130595545538393	4.0913840076063735E-6
32-33	2.577571924792016E-4	3.8459009671499916E-4	0.0	0.012975824380123614	4.0913840076063735E-6
34-35	2.782141125172334E-4	4.398237808176852E-4	0.0	0.022019828728937506	4.0913840076063735E-6
36-37	2.9457964854765894E-4	4.971031569241744E-4	0.0	0.03587734636270029	4.0913840076063735E-6
38-39	2.9457964854765894E-4	5.114230009507967E-4	0.0	0.07519350098379374	4.0913840076063735E-6
40-41	2.9457964854765894E-4	5.809765290801051E-4	0.0	0.11667604343691476	8.182768015212747E-6
42-43	2.9457964854765894E-4	5.932506811029242E-4	0.0	0.14403512829577858	8.182768015212747E-6
44-45	3.0480810856667486E-4	6.709869772474452E-4	0.0	0.17907578662892337	8.182768015212747E-6
46-47	3.150365685856908E-4	7.384948133729505E-4	0.0	0.2146503705750608	8.182768015212747E-6
48-49	3.150365685856908E-4	7.405405053767537E-4	0.0	0.24726483819169498	8.182768015212747E-6
50-51	3.314021046161163E-4	8.141854175136684E-4	0.0	0.2816017784755315	8.182768015212747E-6
52-53	3.4163056463513226E-4	8.223681855288812E-4	0.0	0.3610789585152891	8.182768015212747E-6
54-55	3.436762566389354E-4	8.305509535440939E-4	0.0	0.45804475949556017	8.182768015212747E-6
56-57	3.436762566389354E-4	8.510078735821258E-4	0.0	0.5525475473032522	8.182768015212747E-6
58-59	3.436762566389354E-4	8.550992575897321E-4	0.0	0.6021228473234186	8.182768015212747E-6
60-61	3.477676406465418E-4	8.694191016163544E-4	0.0	0.6304168134280206	8.182768015212747E-6
62-63	3.600417926693609E-4	8.776018696315673E-4	0.0	0.6506814384176949	8.182768015212747E-6
64-65	3.600417926693609E-4	9.982976978559553E-4	0.0	0.6585328043282914	8.182768015212747E-6
66-67	3.6413317667696726E-4	0.0010085261578749712	0.0	0.6660200370622111	8.182768015212747E-6
68-69	3.764073286997864E-4	0.0010330744619206095	0.0	0.6729426588030811	8.182768015212747E-6
70-71	3.907271727264087E-4	0.001051485689954838	0.0	0.6789385820662284	8.182768015212747E-6
72-73	4.1322978476824375E-4	0.001059668457970051	0.0	0.683545480458793	8.182768015212747E-6
74-75	4.1732116877585013E-4	0.0010964909140385082	0.0	0.6870292939412699	8.182768015212747E-6
76-77	4.214125527834565E-4	0.001147633214133588	0.0	0.6904824220436896	8.182768015212747E-6
78-79	4.255039367910629E-4	0.0011660444421678164	0.0	0.6929229326042269	8.182768015212747E-6
80-81	4.255039367910629E-4	0.0011680901341716197	0.0	0.6937882603218356	8.182768015212747E-6
82-83	4.316410128024725E-4	0.0011742272101830293	0.0	0.6944878869871363	8.182768015212747E-6
84-85	4.398237808176852E-4	0.0011905927462134548	0.0	0.694802923555722	8.182768015212747E-6
86-87	4.4391516482529157E-4	0.0012049125902400771	0.0	0.6949727159920377	8.182768015212747E-6
88	4.4596085682909473E-4	0.0012519635063275505	0.0	0.6949993099880871	8.182768015212747E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	30040	0.0	42.3461	1
GTATCAA	51830	0.0	30.89984	1
TCAACGC	58240	0.0	26.65466	4
ATCAACG	58480	0.0	26.552315	3
CAACGCA	59070	0.0	26.266186	5
AACGCAG	60230	0.0	25.801344	6
TATCAAC	63465	0.0	24.754198	2
CGCAGAG	68515	0.0	22.591164	8
ACGCAGA	68620	0.0	22.562645	7
CTTATAC	39920	0.0	21.732685	1
TATACAC	52580	0.0	19.011553	3
GCAGAGT	81280	0.0	18.860956	9
GTACATG	59490	0.0	18.811525	1
TACATGG	58805	0.0	18.525791	2
ACATGGG	58595	0.0	18.163532	3
GAGTACT	50230	0.0	17.703682	12-13
TTATACA	52090	0.0	17.549692	2
AGAGTAC	74545	0.0	17.44887	10-11
CAGAGTA	80575	0.0	17.193521	10-11
TATGCCG	24640	0.0	17.112034	42-43
>>END_MODULE