FastQCFastQC Report
Fri 27 May 2016
SRR2138617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21697473
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA257200.048.4066661
GTATCAA450950.034.3302351
TCAACGC541300.027.6969054
ATCAACG543350.027.5539763
CAACGCA555450.026.9537375
AACGCAG571600.026.1994936
TATCAAC580550.026.0471572
ACGCAGA663450.022.5534737
AGACCGA182050.022.4806888-89
ACCGAGG187050.022.45833890-91
CGCAGAG667200.022.4392818
GACCGAG183750.022.26852290-91
CGAGACC190950.021.48224686-87
GAGACCG186550.021.35767488-89
ACGAGAC198450.020.3894486-87
CACGAGA208650.019.68073384-85
CCCACGA206050.019.5378782-83
GCCCACG206550.018.95901382-83
GTACATG557350.018.9027041
GCAGAGT787900.018.7954399
Produced by FastQC (version 0.11.5)