##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138617_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21697473
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.527385470188165	31.0	31.0	34.0	30.0	34.0
2	31.6376495548583	31.0	31.0	34.0	30.0	34.0
3	31.701613916053727	31.0	31.0	34.0	30.0	34.0
4	35.48925282681536	37.0	35.0	37.0	33.0	37.0
5	35.32507647319114	37.0	35.0	37.0	33.0	37.0
6	35.34808873825998	37.0	35.0	37.0	32.0	37.0
7	35.24625337706377	37.0	35.0	37.0	32.0	37.0
8	35.22363751760401	37.0	35.0	37.0	32.0	37.0
9	36.80036255834953	39.0	37.0	39.0	32.0	39.0
10-11	36.66415874788737	39.0	35.0	39.0	32.0	39.0
12-13	36.62920608312314	39.0	35.0	39.0	32.0	39.0
14-15	37.738748171273215	40.0	37.0	41.0	32.0	41.0
16-17	37.692567424787214	39.5	36.0	41.0	32.0	41.0
18-19	37.71880206971568	39.5	36.5	41.0	32.0	41.0
20-21	37.69160207663796	40.0	37.0	41.0	32.0	41.0
22-23	37.59857822795155	39.0	36.0	41.0	32.0	41.0
24-25	37.53153348265499	39.0	36.0	41.0	32.0	41.0
26-27	37.33251140487485	39.0	36.0	41.0	31.0	41.0
28-29	37.1734218321144	39.0	36.0	41.0	31.0	41.0
30-31	37.01281907560629	39.0	36.0	40.5	30.5	41.0
32-33	36.788821954317065	39.0	35.0	40.0	30.0	41.0
34-35	36.66094481070951	38.5	35.0	40.0	30.0	41.0
36-37	36.51134485198264	38.0	35.0	40.0	30.0	41.0
38-39	36.38570830175891	38.0	35.0	40.0	30.0	41.0
40-41	36.25874084308464	38.0	35.0	40.0	30.0	41.0
42-43	36.010150392974964	38.0	34.5	40.0	29.0	41.0
44-45	35.84597179560171	38.0	34.0	40.0	28.5	41.0
46-47	35.85626648919748	38.0	34.0	40.0	28.5	41.0
48-49	36.15686936601245	38.0	34.5	40.0	29.5	41.0
50-51	36.46305051362616	38.5	35.0	40.0	30.0	41.0
52-53	36.44413108265232	38.5	35.0	40.0	30.0	41.0
54-55	36.35327482509912	38.0	35.0	40.0	29.0	41.0
56-57	36.218982628487105	38.0	34.0	40.0	29.0	41.0
58-59	36.07040297891646	38.0	34.0	40.0	28.5	41.0
60-61	35.88850249023784	38.0	34.0	40.0	28.0	41.0
62-63	35.6863151110787	38.0	34.0	40.0	28.0	41.0
64-65	35.45152586531198	37.0	34.0	40.0	28.0	41.0
66-67	35.21317847890204	37.0	33.0	39.5	28.0	41.0
68-69	34.9362884186737	36.0	33.0	39.0	28.0	41.0
70-71	34.65345691057616	36.0	33.0	39.0	28.0	40.5
72-73	34.3719822306422	35.5	32.5	39.0	27.0	40.0
74-75	34.0617835877582	35.0	32.0	38.0	27.0	40.0
76-77	32.34342571940229	33.5	30.5	36.0	25.5	39.0
78-79	33.35018307898594	35.0	31.5	37.0	26.0	39.0
80-81	33.39226685019661	35.0	32.0	36.0	27.0	39.0
82-83	33.18929130635375	35.0	32.0	36.0	26.5	37.5
84-85	32.96727310774996	34.5	32.0	36.0	26.0	37.0
86-87	32.76259997461969	34.0	32.0	35.0	26.0	37.0
88-89	32.61022101851326	34.0	31.5	35.0	26.0	36.0
90-91	32.502992518542	34.0	31.0	35.0	26.0	36.0
92-93	32.43964486833968	34.0	31.0	35.0	26.5	36.0
94-95	32.41653971686898	34.0	31.0	35.0	27.0	35.5
96-97	32.51536295590361	34.0	31.5	35.0	27.0	35.0
98-99	32.66369223488408	34.0	32.0	35.0	28.5	35.0
100	31.4490972493109	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	10.0
19	52.0
20	302.0
21	1949.0
22	9177.0
23	30041.0
24	74395.0
25	150890.0
26	256376.0
27	381742.0
28	517679.0
29	655651.0
30	791349.0
31	936594.0
32	1106992.0
33	1322438.0
34	1623771.0
35	2035791.0
36	2705114.0
37	3654434.0
38	3744960.0
39	1646982.0
40	50781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.06257779419751	21.631311627856388	14.949386041406756	23.356724536539346
2	14.694281985395502	22.525343603945167	41.0332809218636	21.747093488795727
3	17.711993465782857	27.414238515240925	31.602233126410617	23.271534892565597
4	12.45549193677992	19.283766363023013	39.62018526304883	28.640556437148234
5	12.414717603289564	38.68526533020689	36.484373088055	12.41564397844855
6	28.760151009290347	39.82404310400571	18.933762470864696	12.482043415839254
7	25.123606850620316	33.50046378781774	24.279852233136268	17.096077128425673
8	23.66183379972405	37.8121498296138	21.549610869431664	16.976405501230488
9	25.08210518340085	16.722841411071236	21.57926409218253	36.61578931334538
10-11	23.494041135269107	27.27601316163062	29.97135586839448	19.258589834705795
12-13	24.39506749683442	24.924544170189122	29.849481934799567	20.830906398176893
14-15	21.593833841200652	26.397129813762728	27.25807000217723	24.75096634285939
16-17	21.150342239707236	29.446065718771365	27.904946014710447	21.498646026810952
18-19	21.145011325868264	28.60835950172678	29.575791002386527	20.67083817001843
20-21	22.010095310077517	27.80767370963721	29.375985241763424	20.806245738521852
22-23	21.6963361713982	27.72574154323344	29.263450920492158	21.3144713648762
24-25	21.502211063275784	27.86608852313959	29.310830940516507	21.320869473068118
26-27	21.505504376658024	28.009679277327997	29.1790044537734	21.305811892240577
28-29	21.47487046003442	28.036015196351237	29.184633330891224	21.304481012723112
30-31	21.779070114909672	27.871414166897722	29.09358587612159	21.255929842071016
32-33	21.659623678340708	28.045900491791315	28.97536802925444	21.319107800613537
34-35	21.668844644457337	28.05500642753913	28.995892367489056	21.28025656051447
36-37	21.712227876615955	27.98621736653922	28.986246606681487	21.315308150163336
38-39	21.527336239099085	28.10705914507381	29.025381937722823	21.34022267810429
40-41	21.770875953336432	28.01601879838776	28.854888274388102	21.358216973887703
42-43	21.786158337472404	28.1160758803507	28.58689291620188	21.510872865975024
44-45	21.783411946804158	28.201917953925275	28.44599281560582	21.568677283664744
46-47	21.850559302432686	28.171254296562605	28.201550149590798	21.776636251413912
48-49	21.773046463652054	28.27189110031485	28.248519777240798	21.70654265879229
50-51	21.73203138459736	28.40262617365793	28.12758892393609	21.73775351780862
52-53	21.90188228152254	28.275925568872	28.03379295183556	21.788399197769902
54-55	21.892376255096735	28.175644983201884	28.112055223310307	21.819923538391073
56-57	21.837094197400393	28.251241221050606	28.082259138877795	21.82940544267121
58-59	21.77961125519753	28.23412913918962	28.040804418982646	21.945455186630205
60-61	21.95275969466287	28.190682428555196	28.183174969391523	21.67338290739041
62-63	21.72965427829219	28.28020879569894	28.10577959416592	21.88435733184295
64-65	21.703449651568928	28.27128707007361	28.058779403597654	21.966483874759803
66-67	21.77707306557822	28.34437253648272	28.068830374859626	21.809724023079426
68-69	21.752967741505106	28.481905568481565	27.953622585000275	21.811504105013057
70-71	21.74748110624621	28.223490069117968	27.85541704061181	22.173611784024015
72-73	21.81487329239939	28.233633019650046	27.795322437345355	22.156171250605205
74-75	21.73944939253369	28.344117416370256	27.975008952084945	21.94142423901111
76-77	21.83734533927573	28.087649702182226	27.939596226311693	22.135408732230353
78-79	21.82557699768852	28.1247646326768	27.679689160067845	22.369969209566836
80-81	22.01977551468671	28.279904512278044	27.69801891220839	22.002301060826856
82-83	22.028414106294072	28.084928009311334	27.80846526627581	22.078192618118784
84-85	21.948086169903032	28.025956614582974	27.81086537372351	22.215091841790485
86-87	21.782418222222343	28.2071430971451	27.89496840944873	22.11547027118383
88-89	22.046750790416244	28.221921653361242	27.86613926197391	21.865188294248604
90-91	22.110072774590172	28.149639745533488	27.940150711931256	21.800136767945077
92-93	21.923836004522574	27.86624047212427	27.956616258104056	22.2533072652491
94-95	21.926318187467032	28.111954781914424	28.118667940717796	21.843059089900745
96-97	22.123704834037163	28.044327603884533	28.255096744663682	21.57687081741462
98-99	22.84086921739362	30.13937868610092	28.600616695073438	18.41913540143202
100	22.141466817022806	30.905135674919066	22.37950706255358	24.57389044550455
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4509.0
1	3940.5
2	5361.5
3	7817.0
4	10350.0
5	17001.0
6	23285.5
7	28065.5
8	31396.5
9	31205.5
10	28647.5
11	25655.0
12	27017.0
13	34622.0
14	54845.0
15	84325.0
16	109414.5
17	124764.0
18	131359.5
19	131683.0
20	126726.5
21	119321.5
22	115192.5
23	121254.0
24	141251.0
25	174637.0
26	219096.5
27	269726.0
28	323078.5
29	382535.5
30	440226.0
31	496455.5
32	556143.5
33	610384.5
34	662421.5
35	709773.5
36	756184.5
37	793073.0
38	810592.5
39	826178.0
40	848227.5
41	864307.0
42	872256.0
43	883522.0
44	889126.0
45	889165.5
46	884993.0
47	876671.0
48	866306.5
49	848068.0
50	824327.0
51	791385.0
52	748979.5
53	704862.0
54	658217.5
55	607361.5
56	555610.0
57	502103.0
58	446150.0
59	388891.0
60	327001.0
61	266428.0
62	212590.5
63	166329.5
64	127987.0
65	97457.0
66	74774.0
67	58495.0
68	45909.0
69	34533.5
70	25815.5
71	19624.0
72	14992.5
73	11313.5
74	8407.5
75	6312.0
76	4742.5
77	3410.0
78	2430.0
79	1768.5
80	1258.0
81	918.5
82	682.0
83	472.0
84	318.5
85	209.5
86	143.0
87	115.5
88	86.5
89	67.5
90	55.0
91	42.5
92	34.0
93	21.5
94	11.5
95	8.0
96	7.0
97	6.5
98	3.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.10814162552477886
3	0.0
4	0.0
5	0.0
6	0.0
7	2.3505041347441703E-4
8	0.0
9	0.0
10-11	4.378390054915612E-5
12-13	5.0697148004286025E-5
14-15	9.448104855344215E-4
16-17	1.59004691467988E-4
18-19	8.987221691668887E-5
20-21	7.213320019956698E-4
22-23	4.079760187547267E-4
24-25	3.2971927954170035E-4
26-27	0.0018710366207316026
28-29	0.0036364742943288874
30-31	0.0102588220319165
32-33	0.007796066769496192
34-35	0.007044338992495172
36-37	0.003482964432664274
38-39	6.189151434156995E-4
40-41	1.9193404065845414E-4
42-43	3.487698386136848E-4
44-45	1.687880959253613E-5
46-47	0.0
48-49	3.445281776430596E-5
50-51	0.0
52-53	0.001248838911417904
54-55	5.8783284899775E-4
56-57	7.60979864141934E-4
58-59	4.6164626185914746E-5
60-61	3.387784425191255E-4
62-63	3.8663205055872375E-4
64-65	7.865020621661786E-4
66-67	8.72712925006524E-4
68-69	2.2416292701687217E-4
70-71	2.4881006586002444E-4
72-73	2.7683880208085334E-6
74-75	8.409661052255475E-6
76-77	2.782738830050842E-4
78-79	1.524760777260903E-4
80-81	2.946927097604472E-4
82-83	1.1546777609684987E-4
84-85	4.2065153875534735E-5
86-87	0.0
88-89	6.209387308266927E-6
90-91	0.0
92-93	0.0
94-95	6.304989463367083E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	902.0
20-21	2953.0
22-23	5777.0
24-25	11830.0
26-27	14756.0
28-29	26741.0
30-31	62093.0
32-33	72451.0
34-35	95878.0
36-37	123702.0
38-39	140481.0
40-41	164560.0
42-43	187618.0
44-45	212247.0
46-47	210697.0
48-49	181426.0
50-51	164865.0
52-53	159415.0
54-55	167854.0
56-57	183534.0
58-59	123795.0
60-61	196560.0
62-63	193079.0
64-65	204305.0
66-67	221067.0
68-69	225087.0
70-71	226935.0
72-73	223623.0
74-75	232086.0
76-77	221531.0
78-79	241318.0
80-81	248664.0
82-83	246304.0
84-85	253279.0
86-87	272053.0
88-89	301848.0
90-91	332279.0
92-93	373012.0
94-95	446302.0
96-97	1016743.0
98-99	1489721.0
100-101	1.2218102E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.30866720586435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.50233005102905	75.45822158421639
2	7.234358933555694	12.198383195538375
3	1.7630896551388713	4.459312169676158
4	0.6756044789857922	2.2783725272641813
5	0.31258491210652384	1.3176808664181634
6	0.16337970567803706	0.8264595140521016
7	0.09689888864530845	0.5718591308790791
8	0.06995062490323638	0.4717955164647362
9	0.03764499966661716	0.28564197739719305
>10	0.1414384847338878	1.81634447474938
>50	0.001965701261498615	0.10918092389485068
>100	6.494074161959997E-4	0.0827801919941186
>500	3.8048796689253094E-5	0.020931051656789407
>1k	6.610808267420269E-5	0.10303687579845218
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.6088316367532755E-6
2	0.0	1.8435326547013102E-5	0.0	4.6088316367532755E-6	4.6088316367532755E-6
3	0.0	1.8435326547013102E-5	0.0	4.6088316367532755E-6	4.6088316367532755E-6
4	0.0	7.374130618805241E-5	0.0	9.217663273506551E-6	4.6088316367532755E-6
5	0.0	8.756780109831224E-5	0.0	1.8435326547013102E-5	4.6088316367532755E-6
6	1.8435326547013102E-5	8.756780109831224E-5	0.0	1.8435326547013102E-5	9.217663273506551E-6
7	1.8435326547013102E-5	8.756780109831224E-5	0.0	3.2261821457272926E-5	9.217663273506551E-6
8	2.3044158183766377E-5	9.678546437181878E-5	0.0	5.069714800428603E-5	9.217663273506551E-6
9	2.3044158183766377E-5	1.7974443383337774E-4	0.0	9.678546437181878E-5	1.3826494910259826E-5
10-11	3.2261821457272926E-5	1.7974443383337774E-4	0.0	1.290472858290917E-4	1.6130910728636463E-5
12-13	3.2261821457272926E-5	1.7974443383337774E-4	0.0	1.7283118637824782E-4	1.8435326547013102E-5
14-15	4.1479484730779475E-5	2.5118132420305346E-4	0.0	1.7974443383337774E-4	2.765298982051965E-5
16-17	5.53059796410393E-5	2.7192106656844325E-4	0.0	2.073974236538974E-4	3.2261821457272926E-5
18-19	6.913247455129912E-5	3.110961354808461E-4	0.0	2.3274599765604043E-4	3.2261821457272926E-5
20-21	7.604572200642904E-5	3.5027120439324894E-4	0.0	3.134005512992227E-4	3.2261821457272926E-5
22-23	7.835013782480568E-5	3.710109467586387E-4	0.0	5.553642122287696E-4	3.917506891240284E-5
24-25	9.217663273506551E-5	3.9866393657915836E-4	0.0	0.0012121227204661116	4.839273218590939E-5
26-27	1.0830754346370197E-4	4.2862134221805464E-4	0.0	0.0032584439671845657	5.53059796410393E-5
28-29	1.106119592820786E-4	4.332301738548079E-4	0.0	0.011259375688588251	5.53059796410393E-5
30-31	1.106119592820786E-4	4.7470965858558735E-4	0.0	0.014633040446691649	5.53059796410393E-5
32-33	1.1752520673720851E-4	4.7931849022234065E-4	0.0	0.02143798035835786	5.991481127779258E-5
34-35	1.405693649209749E-4	5.691907071390294E-4	0.0	0.029634787424323558	7.835013782480568E-5
36-37	1.4748261237610482E-4	6.452364291454585E-4	0.0	0.044041995120814297	7.835013782480568E-5
38-39	1.4748261237610482E-4	6.590629240557184E-4	0.0	0.0768384410479506	7.835013782480568E-5
40-41	1.659179389231179E-4	7.212821511518876E-4	0.0	0.11035386471042043	8.526338527993559E-5
42-43	1.659179389231179E-4	7.489351409724072E-4	0.0	0.13402251958096686	8.756780109831224E-5
44-45	1.6822235474149455E-4	8.526338527993559E-4	0.0	0.16432789200843803	8.987221691668887E-5
46-47	1.7513560219662447E-4	9.28679574805785E-4	0.0	0.1956886868807257	9.448104855344214E-5
48-49	1.7513560219662447E-4	9.355928222609149E-4	0.0	0.2252289932564958	1.106119592820786E-4
50-51	1.8204884965175436E-4	0.001073857771363513	0.0	0.25659439696042025	1.106119592820786E-4
52-53	1.8435326547013101E-4	0.0010922930979105263	0.0	0.3247290594623623	1.1291637510045524E-4
54-55	1.8435326547013101E-4	0.001101510761184033	0.0	0.40923429193805194	1.1752520673720853E-4
56-57	1.8435326547013101E-4	0.0011245549193677992	0.0	0.4941658413401413	1.221340383739618E-4
58-59	1.958753445620142E-4	0.0011314681668229292	0.0	0.7053378981045396	1.2443845419233843E-4
60-61	2.0509300783552075E-4	0.001152207909188319	0.0	0.8834369790435964	1.3365611746584498E-4
62-63	2.1661508692740394E-4	0.0011683388199169552	0.0	0.8925762921792781	1.3365611746584498E-4
64-65	2.1661508692740394E-4	0.0013273435113849432	0.0	0.8994296248231304	1.3365611746584498E-4
66-67	2.212239185641572E-4	0.0013526920853870862	0.0	0.9059234685993157	1.3365611746584498E-4
68-69	2.3044158183766378E-4	0.0013780406593892293	0.0	0.9120555190920159	1.3365611746584498E-4
70-71	2.3044158183766378E-4	0.0013895627384811126	0.0	0.9170353616755278	1.3826494910259827E-4
72-73	2.580945716581834E-4	0.0014010848175729958	0.0	0.9209897392198622	1.659179389231179E-4
74-75	2.580945716581834E-4	0.001431042223211892	0.0	0.9240960917430339	1.659179389231179E-4
76-77	2.6039898747656006E-4	0.0014725217079426716	0.0	0.9271632691972931	1.659179389231179E-4
78-79	2.627034032949367E-4	0.0014932614503080613	0.0	0.9292072860281932	1.659179389231179E-4
80-81	2.627034032949367E-4	0.0014955658661264378	0.0	0.9299884829906229	1.7283118637824782E-4
82-83	2.627034032949367E-4	0.0015024791135815677	0.0	0.9305023677181209	1.8204884965175436E-4
84-85	2.627034032949367E-4	0.0015232188559469576	0.0	0.9307489402106872	1.8435326547013101E-4
86-87	2.6731223493168995E-4	0.0015600895090409836	0.0	0.9308411168434223	1.8435326547013101E-4
88	2.7192106656844325E-4	0.0016084822412268931	0.0	0.9308457256750591	1.8896209710688429E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25720	0.0	48.406666	1
GTATCAA	45095	0.0	34.330235	1
TCAACGC	54130	0.0	27.696905	4
ATCAACG	54335	0.0	27.553976	3
CAACGCA	55545	0.0	26.953737	5
AACGCAG	57160	0.0	26.199493	6
TATCAAC	58055	0.0	26.047157	2
ACGCAGA	66345	0.0	22.553473	7
AGACCGA	18205	0.0	22.48068	88-89
ACCGAGG	18705	0.0	22.458338	90-91
CGCAGAG	66720	0.0	22.439281	8
GACCGAG	18375	0.0	22.268522	90-91
CGAGACC	19095	0.0	21.482246	86-87
GAGACCG	18655	0.0	21.357674	88-89
ACGAGAC	19845	0.0	20.38944	86-87
CACGAGA	20865	0.0	19.680733	84-85
CCCACGA	20605	0.0	19.53787	82-83
GCCCACG	20655	0.0	18.959013	82-83
GTACATG	55735	0.0	18.902704	1
GCAGAGT	78790	0.0	18.795439	9
>>END_MODULE