FastQCFastQC Report
Fri 27 May 2016
SRR2138616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20970448
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA263250.047.2173961
ATCTCGT187600.044.0633594
TATCTCG232150.035.54364494
GTATCAA453350.033.765271
TATGCCG103600.027.66459886-87
ATCAACG559000.026.713443
TCAACGC559350.026.6292764
GCCGTCT118050.026.39167490-91
CAACGCA567850.026.2232785
CCGTCTT114400.026.20193190-91
ATGCCGT115150.026.05617188-89
AACGCAG580000.025.710126
TGCCGTC113850.025.53591288-89
TATCAAC598650.025.1139012
CGCTATC352550.024.33033694
TCGTATG126400.024.0819784-85
TCTCGTA123000.023.94682182-83
CTCGTAT121950.023.60040982-83
CGTATGC128550.022.8772784-85
CTATCTC373200.022.84502894
Produced by FastQC (version 0.11.5)