FastQCFastQC Report
Fri 27 May 2016
SRR2138615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9015321
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100550.048.803661
GTATCAA179600.034.147891
TCAACGC218000.027.284684
ATCAACG219600.027.0858843
CAACGCA226100.026.2700795
AACGCAG232100.025.6994866
TATCAAC236700.025.378232
ACGCAGA269950.022.12767
CGCAGAG272250.021.8789868
GTACATG232550.019.1522141
TACATGG231050.018.7315032
AGAGTAC292800.018.35782410-11
GCAGAGT323900.018.1957059
ACATGGG231250.018.0975133
GAGTACT192350.017.97169112-13
CAGAGTA316150.016.80280110-11
TATACCG6900.016.4255585
CATGGGG171050.016.049454
GTGGTAT48800.015.8277031
AGTACTT220600.015.80344112-13
Produced by FastQC (version 0.11.5)