FastQCFastQC Report
Fri 27 May 2016
SRR2138614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5508844
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63200.053.976381
GTATCAA121050.038.049531
ATCAACG157750.028.7407513
TCAACGC158400.028.597044
TATCAAC163000.028.0505852
CAACGCA161200.027.9959535
AACGCAG163300.027.3783746
ACGCAGA192000.023.2858757
CGCAGAG197600.022.3066798
GTACATG159500.019.4096241
GCAGAGT224250.019.3931629
GTGGTAT27600.018.5930541
TACATGG161050.018.439282
AGAGTAC203650.018.07083710-11
GAGTACT133100.018.0113212-13
ACATGGG159450.017.857183
CAGAGTA221250.016.89947110-11
AACGCCG4101.2732926E-1116.413337
TATGCCG20100.016.30699242-43
GTACTTT148400.016.29172514-15
Produced by FastQC (version 0.11.5)