FastQCFastQC Report
Fri 27 May 2016
SRR2138613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23359263
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA274750.050.1167071
GTATCAA500950.034.6362951
TCAACGC611800.027.6619744
ATCAACG615800.027.4822923
CAACGCA621550.027.2214285
AACGCAG641550.026.4626486
TATCAAC651150.026.2100522
ACGCAGA739100.022.91997
CGCAGAG751900.022.5297248
GTACATG648700.019.136161
GCAGAGT879250.018.906389
TACATGG645000.018.6708552
GAGTACT518200.018.32366612-13
GTGGTAT130650.018.2016221
ACATGGG645100.018.187083
AGAGTAC795250.018.1442810-11
CAGAGTA867100.017.36008310-11
TGGTATC124950.016.9209522
CATGGGG464150.016.283944
AGTACTT582500.015.9052112-13
Produced by FastQC (version 0.11.5)