FastQCFastQC Report
Fri 27 May 2016
SRR2138612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9154205
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99900.048.384211
GTATCAA185750.034.503051
TCAACGC228000.027.256154
ATCAACG229800.027.0786423
CAACGCA234200.026.5875595
AACGCAG238200.026.17586
TATCAAC245150.025.6875482
ACGCAGA274400.022.6782427
CGCAGAG277550.022.4208588
GTACATG272250.019.8197061
TACATGG265700.019.7636342
ACATGGG265350.019.041123
GCAGAGT325250.018.8530169
AGAGTAC301200.018.33352310-11
CATGGGG178250.017.7216514
GAGTACT175600.017.25982712-13
CAGAGTA325550.016.53040310-11
AGTACTT201550.015.91973812-13
GTACTTT202150.015.00326314-15
TATACAG97050.014.9964165
Produced by FastQC (version 0.11.5)