##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138612_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9154205
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79989655027389	31.0	31.0	34.0	30.0	34.0
2	32.01206538415952	31.0	31.0	34.0	30.0	34.0
3	32.028707790572746	31.0	31.0	34.0	30.0	34.0
4	35.771387466197226	37.0	35.0	37.0	35.0	37.0
5	35.62915774772359	37.0	35.0	37.0	33.0	37.0
6	35.62875574667598	37.0	35.0	37.0	33.0	37.0
7	35.501321523824295	37.0	35.0	37.0	33.0	37.0
8	35.47888068925701	37.0	35.0	37.0	33.0	37.0
9	37.02806447965716	39.0	37.0	39.0	33.0	39.0
10-11	36.95552022267363	39.0	37.0	39.0	33.0	39.0
12-13	36.894557146142134	39.0	37.0	39.0	33.0	39.0
14-15	37.995924714379896	40.0	37.0	41.0	33.0	41.0
16-17	38.04471354967471	40.0	37.0	41.0	33.0	41.0
18-19	38.085449528386135	40.0	37.0	41.0	33.0	41.0
20-21	38.06901067349209	40.0	37.0	41.0	33.0	41.0
22-23	38.01183917950736	40.0	37.0	41.0	33.0	41.0
24-25	37.92469525685232	40.0	37.0	41.0	33.0	41.0
26-27	37.78186018534524	39.5	37.0	41.0	32.5	41.0
28-29	37.65136660874347	39.0	37.0	41.0	32.0	41.0
30-31	37.508508237507115	39.0	36.0	41.0	32.0	41.0
32-33	37.71498258675234	39.5	37.0	41.0	33.0	41.0
34-35	37.71431427418973	40.0	37.0	41.0	33.0	41.0
36-37	37.60366044873622	39.5	36.5	41.0	32.0	41.0
38-39	37.494537125741715	39.0	36.0	41.0	32.0	41.0
40-41	37.483068509451996	39.0	36.0	41.0	32.0	41.0
42-43	37.417168523300646	39.0	36.0	41.0	32.0	41.0
44-45	37.38569984054992	39.0	36.0	41.0	31.5	41.0
46-47	37.39984776209636	39.0	36.0	41.0	31.5	41.0
48-49	37.32893814539297	39.0	36.0	41.0	31.0	41.0
50-51	37.26072927883622	39.0	36.0	41.0	31.0	41.0
52-53	37.15273785494014	39.0	36.0	40.0	31.0	41.0
54-55	37.054787137575	39.0	35.5	40.0	31.0	41.0
56-57	36.895246443248155	39.0	35.0	40.0	31.0	41.0
58-59	36.76228684974653	38.5	35.0	40.0	31.0	41.0
60-61	36.61737781774486	38.0	35.0	40.0	30.5	41.0
62-63	36.40862193716998	38.0	35.0	40.0	30.0	41.0
64-65	36.20705154474988	38.0	34.0	40.0	30.0	41.0
66-67	35.96775474565593	37.5	34.0	40.0	29.5	41.0
68-69	35.70412412957688	37.0	34.0	39.5	29.0	41.0
70-71	35.438705208124375	36.5	34.0	39.0	29.0	41.0
72-73	35.141300078161805	36.0	34.0	39.0	29.0	40.0
74-75	34.82060671097238	35.5	33.5	38.5	29.0	40.0
76-77	33.75013749616406	35.0	32.0	37.0	27.5	39.0
78-79	34.107801028042545	35.0	33.0	37.0	29.0	39.0
80-81	34.0126230633806	35.0	33.0	37.0	29.0	39.0
82-83	33.78358505689161	35.0	33.0	36.0	29.0	38.5
84-85	33.51637318938266	35.0	33.0	36.0	29.0	37.0
86-87	33.311228424550166	35.0	33.0	35.5	29.0	37.0
88-89	33.090222678754394	34.5	32.0	35.0	29.0	36.5
90-91	32.96736025661639	34.0	32.0	35.0	29.0	36.0
92-93	32.9001685803366	34.0	32.0	35.0	29.0	36.0
94-95	32.86181474229379	34.0	32.0	35.0	29.0	36.0
96-97	32.751784416653734	34.0	32.0	35.0	29.0	35.0
98-99	32.86046311824184	34.0	32.0	35.0	29.0	35.0
100-101	32.40390080922448	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	53.0
21	313.0
22	1569.0
23	5282.0
24	14064.0
25	29659.0
26	54532.0
27	87949.0
28	129808.0
29	177177.0
30	232763.0
31	298597.0
32	379264.0
33	486006.0
34	635421.0
35	841615.0
36	1181154.0
37	1703517.0
38	1909823.0
39	950067.0
40	35567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.85762990887794	23.730274775362798	16.170371976594364	23.241723339164896
2	13.315696311858256	23.346239741066356	42.46711029052144	20.870953656553947
3	15.926746233015319	29.124375082270937	32.74343320910991	22.205445475603835
4	11.262092120506368	20.12928484778307	41.26542938463798	27.34319364707257
5	11.553051302652715	39.43283988068871	37.277513448737494	11.736595367921081
6	27.040840793930222	41.47510351800074	19.588833765466255	11.895221922602781
7	23.930095513482602	34.21257225504563	25.51690725737516	16.340424974096603
8	22.698759750300546	39.13257350037496	22.036473948311187	16.132192801013304
9	24.676834307293753	17.145246364921913	22.39229949515004	35.78561983263429
10-11	22.878327500858894	28.061087773323845	30.644348690028245	18.416236035789016
12-13	23.68832902771966	25.526826157076894	30.638117672402565	20.146727142800884
14-15	20.934570506122597	27.036159884992745	27.958528348447516	24.070741260437142
16-17	20.3960201896287	30.1746629008199	28.553129408834522	20.876187500716885
18-19	20.4641680004859	29.412674931320872	30.136072502087575	19.987084566105654
20-21	21.443009135765987	28.509931468782423	29.8079907842131	20.239068611238487
22-23	21.07419589686883	28.367605485999054	29.766441174415604	20.791757442716513
24-25	20.7883462589071	28.6108859818452	29.810705825007343	20.79006193424035
26-27	20.860737808842696	28.709262254018398	29.689867967107798	20.74013197003111
28-29	20.842068948883885	28.762108198926313	29.696385871100407	20.6994369810894
30-31	21.191605975606546	28.655983521519563	29.447705557878727	20.704704944995164
32-33	21.0314750537644	28.850034972038014	29.41374810834564	20.70474186585195
34-35	20.986774651470384	28.791630003006514	29.48367287828913	20.737922467233975
36-37	21.025533356291536	28.64696913243056	29.588950931625153	20.73854657965275
38-39	20.87656416853003	28.791033995376324	29.648381156631988	20.684020679461657
40-41	21.080464389538236	28.693894028081264	29.51040693480225	20.71523464757825
42-43	21.095743586444524	28.814495118575994	29.168529785769408	20.921231509210074
44-45	21.09866310844764	28.98407132424391	28.9987521841644	20.91851338314405
46-47	21.27519955136427	28.76504673481944	28.745772315050733	21.213981398765558
48-49	21.154609071323918	28.87623335263683	28.87068904231534	21.098468533723914
50-51	21.060459147948574	28.963990347264616	28.734945603354927	21.24060490143188
52-53	21.255825972856993	28.897244586205833	28.589863787893716	21.257065653043462
54-55	21.31370361325687	28.76002736806875	28.640345024074154	21.28592399460022
56-57	21.387066244453617	28.882505117701466	28.540380046851467	21.190048590993445
58-59	21.307927482009536	28.92751957296212	28.558716158979824	21.205836786048522
60-61	21.337804264777454	28.978925074401314	28.514485382227413	21.16878527859382
62-63	21.35815904911171	28.97631995675793	28.404242187640033	21.261278806490328
64-65	21.285425650745584	28.917455104157636	28.455154848874486	21.341964396222295
66-67	21.43627399649652	28.928191187296036	28.283606009699046	21.351928806508393
68-69	21.329010846252963	28.94181515388868	28.34450356802718	21.38467043183118
70-71	21.38337259482456	28.530161423941202	28.508755204194493	21.577710777039748
72-73	21.446777257465232	28.679854440010082	28.448511665353177	21.424856637171512
74-75	21.16653220425346	28.87420632250906	28.440048053539385	21.51921341969809
76-77	21.360434797805038	28.824074656600608	28.38970326098354	21.425787284610813
78-79	21.34374568269984	28.893155464528547	28.246693094427172	21.51640575834444
80-81	21.454866433748666	28.772864416062067	28.256592264803803	21.515676885385464
82-83	21.390099376731072	28.709481231125356	28.431746783275386	21.468672608868186
84-85	21.559990459129434	28.64881854231384	28.31897148163446	21.472219516922266
86-87	21.265750850658964	28.806226965224596	28.373633059525567	21.554389124590877
88-89	21.483801811340722	28.700003977512562	28.28782267570662	21.5283715354401
90-91	21.462691740316806	28.690434984538854	28.451435684811592	21.395437590332747
92-93	21.480486184867047	28.741677389130793	28.2322694295863	21.545566996415864
94-95	21.239817462370546	28.731552835809094	28.36554882428698	21.663080877533382
96-97	21.18680791103988	28.8494440161362	28.44161616577673	21.52213190704719
98-99	21.312196382771443	29.505839833696733	28.512341301663252	20.669622481868572
100-101	24.942977801629905	36.576344289620785	29.402186757849336	9.078491150899971
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2326.0
1	2066.0
2	2675.5
3	3692.0
4	4570.5
5	6497.0
6	8107.5
7	9449.0
8	10394.0
9	10274.5
10	9588.5
11	8963.5
12	9942.5
13	13261.0
14	21198.0
15	32588.5
16	41915.0
17	46770.0
18	48103.0
19	47303.5
20	45240.5
21	43526.5
22	44212.0
23	50161.5
24	62399.0
25	82202.5
26	109800.5
27	140893.0
28	177947.0
29	219267.5
30	255062.0
31	290044.0
32	326875.5
33	362296.0
34	395646.5
35	419973.5
36	437016.0
37	444928.5
38	435933.5
39	417156.0
40	403114.0
41	388032.5
42	370108.5
43	356485.0
44	342601.0
45	329187.0
46	317998.5
47	309133.5
48	300116.5
49	289464.0
50	279782.0
51	271047.5
52	258071.0
53	242794.0
54	227507.0
55	210425.0
56	193004.5
57	174568.0
58	155150.0
59	135429.5
60	114506.0
61	93942.0
62	75180.0
63	58602.5
64	44293.5
65	33266.5
66	25419.0
67	19644.0
68	15164.5
69	11342.5
70	8288.5
71	5987.0
72	4435.0
73	3264.0
74	2378.0
75	1688.5
76	1187.0
77	812.5
78	561.5
79	373.0
80	240.5
81	165.5
82	123.5
83	92.0
84	70.0
85	53.5
86	46.0
87	41.0
88	30.5
89	17.5
90	7.5
91	4.0
92	2.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003867075294905456
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	7.701378765277815E-4
14-15	0.0
16-17	0.0
18-19	4.478816019523268E-4
20-21	0.0
22-23	0.0013820730593317955
24-25	2.1309297902345498E-4
26-27	0.004930253043870999
28-29	3.390779147998923E-4
30-31	0.0
32-33	0.0
34-35	1.7069910257975352E-4
36-37	0.021754464354131697
38-39	0.008766155040123968
40-41	0.013849569626730781
42-43	0.0017570493813060715
44-45	0.0028169967276460576
46-47	0.0
48-49	7.384544839219997E-4
50-51	3.5558579351784877E-5
52-53	2.9943956890284147E-5
54-55	0.004345294007252526
56-57	0.008889710105451431
58-59	0.012395633415008161
60-61	0.006364878125451749
62-63	0.007112011641072852
64-65	9.391943693625019E-4
66-67	1.3032305325540796E-4
68-69	6.606478563925971E-6
70-71	1.3404001912214913E-4
72-73	6.799199380674527E-6
74-75	1.8611475049697807E-4
76-77	6.291282610077E-5
78-79	2.1287145714486018E-5
80-81	0.0021637695115210988
82-83	0.0024273948597017107
84-85	0.010021231599677652
86-87	0.011100822195344332
88-89	0.04434962917669336
90-91	4.6349450503696765E-4
92-93	7.139345341284372E-4
94-95	1.640905408941113E-5
96-97	0.009697188617070343
98-99	9.720160124615987E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	206.0
20-21	765.0
22-23	1540.0
24-25	3102.0
26-27	4453.0
28-29	8009.0
30-31	20538.0
32-33	26856.0
34-35	37866.0
36-37	60798.0
38-39	72619.0
40-41	75715.0
42-43	84933.0
44-45	100065.0
46-47	101344.0
48-49	95582.0
50-51	89747.0
52-53	85529.0
54-55	92891.0
56-57	99400.0
58-59	100752.0
60-61	100797.0
62-63	94456.0
64-65	96690.0
66-67	104844.0
68-69	106800.0
70-71	108595.0
72-73	101042.0
74-75	100134.0
76-77	103788.0
78-79	113070.0
80-81	116035.0
82-83	108078.0
84-85	106588.0
86-87	111717.0
88-89	121959.0
90-91	130764.0
92-93	135148.0
94-95	161467.0
96-97	206642.0
98-99	707477.0
100-101	5055404.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.77787573347979
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.04411752732004	77.23793123789308
2	6.9968616129197425	12.003518519147693
3	1.6788288946069139	4.320191288981013
4	0.6048840600870066	2.0754267895722385
5	0.26508806380742767	1.1369345497851144
6	0.1486450197307625	0.7650272418519598
7	0.08043015364100388	0.4829389406969903
8	0.04807823729481829	0.3299239251327742
9	0.03593361593313982	0.27740783179507106
>10	0.09530780998124187	1.1906998040348769
>50	0.0012865193918473383	0.06975939922383378
>100	4.6022532400631394E-4	0.059726269358058914
>500	5.869620241396678E-5	0.029218634457593772
>1k	1.9563759738733204E-5	0.02129556806969115
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0923941511032361E-5
2	0.0	3.277182453309709E-5	0.0	0.0	1.0923941511032361E-5
3	0.0	3.277182453309709E-5	0.0	0.0	1.0923941511032361E-5
4	0.0	1.0923941511032361E-4	0.0	1.0923941511032361E-5	1.0923941511032361E-5
5	0.0	1.0923941511032361E-4	0.0	1.0923941511032361E-5	1.0923941511032361E-5
6	2.1847883022064722E-5	1.0923941511032361E-4	0.0	2.1847883022064722E-5	2.1847883022064722E-5
7	2.1847883022064722E-5	1.0923941511032361E-4	0.0	2.1847883022064722E-5	2.1847883022064722E-5
8	2.1847883022064722E-5	1.3108729813238835E-4	0.0	2.1847883022064722E-5	2.1847883022064722E-5
9	2.1847883022064722E-5	2.1847883022064723E-4	0.0	1.2016335662135598E-4	2.1847883022064722E-5
10-11	2.1847883022064722E-5	2.1847883022064723E-4	0.0	1.2016335662135598E-4	2.1847883022064722E-5
12-13	2.1847883022064722E-5	2.294027717316796E-4	0.0	1.2016335662135598E-4	2.1847883022064722E-5
14-15	2.1847883022064722E-5	2.676365670202929E-4	0.0	1.3108729813238835E-4	3.277182453309709E-5
16-17	2.1847883022064722E-5	2.8402247928684143E-4	0.0	1.4201123964342071E-4	3.277182453309709E-5
18-19	3.277182453309709E-5	3.4410415759751937E-4	0.0	1.5293518115445305E-4	4.3695766044129444E-5
20-21	3.277182453309709E-5	4.151097774192297E-4	0.0	1.638591226654854E-4	4.3695766044129444E-5
22-23	3.823379528861327E-5	4.4241963119681063E-4	0.0	2.0209291795409868E-4	4.9157736799645625E-5
24-25	4.3695766044129444E-5	5.13425251018521E-4	0.0	2.1847883022064723E-4	5.4619707555161806E-5
26-27	4.3695766044129444E-5	5.571210170626505E-4	0.0	3.167943038199385E-4	5.4619707555161806E-5
28-29	4.3695766044129444E-5	5.571210170626505E-4	0.0	9.285350284377507E-4	5.4619707555161806E-5
30-31	4.3695766044129444E-5	5.898928415957475E-4	0.0	0.00296038814948977	5.4619707555161806E-5
32-33	4.3695766044129444E-5	5.898928415957475E-4	0.0	0.008487902554072144	5.4619707555161806E-5
34-35	4.3695766044129444E-5	6.554364906619417E-4	0.0	0.01585610110326347	5.4619707555161806E-5
36-37	4.3695766044129444E-5	7.264421104836521E-4	0.0	0.025534713282038143	5.4619707555161806E-5
38-39	4.3695766044129444E-5	7.482899935057168E-4	0.0	0.04000347381340051	5.4619707555161806E-5
40-41	4.3695766044129444E-5	7.919857595498462E-4	0.0	0.055985200244040856	6.554364906619418E-5
42-43	4.3695766044129444E-5	8.192956133274271E-4	0.0	0.0697712144309637	6.554364906619418E-5
44-45	4.9157736799645625E-5	8.793772916381051E-4	0.0	0.08384671306792889	6.554364906619418E-5
46-47	5.4619707555161806E-5	9.394589699487831E-4	0.0	0.09766549907938482	6.554364906619418E-5
48-49	5.4619707555161806E-5	9.613068529708479E-4	0.0	0.10884615321592644	6.554364906619418E-5
50-51	5.4619707555161806E-5	0.0011579378001694302	0.0	0.11996126370340188	6.554364906619418E-5
52-53	5.4619707555161806E-5	0.001179785683191495	0.0	0.12956887026235483	6.554364906619418E-5
54-55	6.0081678310677995E-5	0.001179785683191495	0.0	0.13868489945331136	7.100561982171036E-5
56-57	6.554364906619418E-5	0.0011907096247025274	0.0	0.14729842733476037	8.19295613327427E-5
58-59	6.554364906619418E-5	0.0011961715954580435	0.0	0.15567162850296667	8.739153208825889E-5
60-61	6.554364906619418E-5	0.0012016335662135597	0.0	0.16393012828530712	8.739153208825889E-5
62-63	7.646759057722652E-5	0.001207095536969076	0.0	0.17150041975245256	8.739153208825889E-5
64-65	7.646759057722652E-5	0.0014091884549231747	0.0	0.17952951676306134	8.739153208825889E-5
66-67	9.831547359929125E-5	0.001420112396434207	0.0	0.18750399406611495	8.739153208825889E-5
68-69	9.831547359929125E-5	0.0014528842209673041	0.0	0.19604651632774228	8.739153208825889E-5
70-71	9.831547359929125E-5	0.0014692701332338527	0.0	0.20468189209221335	8.739153208825889E-5
72-73	9.831547359929125E-5	0.0014747321039893688	0.0	0.21184253575269507	1.2016335662135598E-4
74-75	9.831547359929125E-5	0.0014911180162559172	0.0	0.21784524161300733	1.2016335662135598E-4
76-77	9.831547359929125E-5	0.0015457377238110792	0.0	0.22325805463172388	1.2016335662135598E-4
78-79	9.831547359929125E-5	0.0015511996945665953	0.0	0.22759485941160373	1.2016335662135598E-4
80-81	9.831547359929125E-5	0.0015511996945665953	0.0	0.23047331799976076	1.3108729813238835E-4
82-83	9.831547359929125E-5	0.0015511996945665953	0.0	0.23229215426134764	1.4201123964342071E-4
84-85	9.831547359929125E-5	0.0015675856068331439	0.0	0.23418745811351177	1.4201123964342071E-4
86-87	9.831547359929125E-5	0.00157304757758866	0.0	0.23451517635884273	1.4201123964342071E-4
88-89	9.831547359929125E-5	0.0016713630511879514	0.0	0.23458072000790894	1.4201123964342071E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9990	0.0	48.38421	1
GTATCAA	18575	0.0	34.50305	1
TCAACGC	22800	0.0	27.25615	4
ATCAACG	22980	0.0	27.078642	3
CAACGCA	23420	0.0	26.587559	5
AACGCAG	23820	0.0	26.1758	6
TATCAAC	24515	0.0	25.687548	2
ACGCAGA	27440	0.0	22.678242	7
CGCAGAG	27755	0.0	22.420858	8
GTACATG	27225	0.0	19.819706	1
TACATGG	26570	0.0	19.763634	2
ACATGGG	26535	0.0	19.04112	3
GCAGAGT	32525	0.0	18.853016	9
AGAGTAC	30120	0.0	18.333523	10-11
CATGGGG	17825	0.0	17.721651	4
GAGTACT	17560	0.0	17.259827	12-13
CAGAGTA	32555	0.0	16.530403	10-11
AGTACTT	20155	0.0	15.919738	12-13
GTACTTT	20215	0.0	15.003263	14-15
TATACAG	9705	0.0	14.996416	5
>>END_MODULE