FastQCFastQC Report
Fri 27 May 2016
SRR2138611_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138611_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21279101
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA228700.047.3098261
GTATCAA396400.035.361921
TCAACGC499650.027.2670884
ATCAACG499650.027.2586213
CAACGCA512050.026.6150385
TATCAAC527050.026.234942
AACGCAG528950.025.8846326
ACGCAGA617950.022.1223857
CGCAGAG627650.021.800718
GTACATG629700.021.3201481
TACATGG628250.020.8980712
ACATGGG638600.019.9829833
AGAGTAC661700.018.21467610-11
CATGGGG465950.018.1008824
GCAGAGT753800.017.9503129
GAGTACT384600.017.46445812-13
CAGAGTA727550.016.60386710-11
AGTACTT430850.015.93823112-13
TATACTG114100.015.310115
GTACTTT441400.015.22742314-15
Produced by FastQC (version 0.11.5)