FastQCFastQC Report
Fri 27 May 2016
SRR2138610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22776539
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA266100.046.056681
GTATCAA464800.033.468371
ATCAACG558350.027.066793
TCAACGC561850.026.8832244
CAACGCA570200.026.4972615
AACGCAG583900.025.9763286
TATCAAC595250.025.6039852
ACGCAGA668900.022.5816577
CGCAGAG676950.022.3069218
GTACATG608750.020.2430441
TACATGG612700.019.6459242
ACATGGG620250.018.907353
GCAGAGT810850.018.441959
AGAGTAC727050.018.1737310-11
GAGTACT485400.017.71629312-13
CATGGGG436300.016.9822584
CAGAGTA795100.016.96957810-11
AGTACTT535050.015.742619512-13
GTACTTT558300.015.39600814-15
GTGGTAT136300.014.4669161
Produced by FastQC (version 0.11.5)