##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138610_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22776539
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.448582903662405	31.0	31.0	34.0	30.0	34.0
2	31.56114232280857	31.0	31.0	34.0	30.0	34.0
3	31.64473478608844	31.0	31.0	34.0	30.0	34.0
4	35.443292635461425	37.0	35.0	37.0	33.0	37.0
5	35.245316551386495	37.0	35.0	37.0	32.0	37.0
6	35.254968061653265	37.0	35.0	37.0	32.0	37.0
7	35.16769913989127	37.0	35.0	37.0	32.0	37.0
8	35.093502046118594	37.0	35.0	37.0	32.0	37.0
9	36.63806849671059	39.0	35.0	39.0	32.0	39.0
10-11	36.56790241484889	39.0	35.0	39.0	32.0	39.0
12-13	36.53514800909831	39.0	35.0	39.0	32.0	39.0
14-15	37.63200576698681	39.0	36.0	41.0	32.0	41.0
16-17	37.5969719323906	39.0	36.0	41.0	32.0	41.0
18-19	37.61403045036825	39.0	36.0	41.0	32.0	41.0
20-21	37.61875302953028	39.0	36.0	41.0	32.0	41.0
22-23	37.52330529326156	39.0	36.0	41.0	32.0	41.0
24-25	37.351098330639516	39.0	36.0	41.0	31.0	41.0
26-27	37.2207836402326	39.0	36.0	41.0	31.0	41.0
28-29	37.06022489681044	39.0	36.0	41.0	31.0	41.0
30-31	36.92966935031144	39.0	36.0	40.0	30.0	41.0
32-33	36.776810693966844	39.0	35.0	40.0	30.0	41.0
34-35	36.63275474823652	38.0	35.0	40.0	30.0	41.0
36-37	36.48765375164275	38.0	35.0	40.0	30.0	41.0
38-39	36.37297274088029	38.0	35.0	40.0	30.0	41.0
40-41	36.25416328771844	38.0	35.0	40.0	30.0	41.0
42-43	36.10182188842455	38.0	34.5	40.0	29.0	41.0
44-45	35.95369003736166	38.0	34.0	40.0	29.0	41.0
46-47	36.101689109145724	38.0	34.0	40.0	29.0	41.0
48-49	36.249726353941796	38.0	34.5	40.0	29.5	41.0
50-51	36.464382583720564	38.0	35.0	40.0	30.0	41.0
52-53	36.444958930245164	38.0	35.0	40.0	30.0	41.0
54-55	36.351093929419065	38.0	35.0	40.0	29.5	41.0
56-57	36.20404630726845	38.0	34.0	40.0	29.0	41.0
58-59	36.01636420744373	38.0	34.0	40.0	28.5	41.0
60-61	35.84960767337371	38.0	34.0	40.0	28.0	41.0
62-63	35.650651183205326	37.5	34.0	40.0	28.0	41.0
64-65	35.43851127484008	37.0	34.0	40.0	28.0	41.0
66-67	35.19697211230404	37.0	33.0	39.5	28.0	41.0
68-69	34.93007799709836	36.0	33.0	39.0	28.0	41.0
70-71	34.61771165148049	36.0	33.0	39.0	28.0	40.5
72-73	34.35766409555939	35.0	32.5	38.5	27.5	40.0
74-75	33.997318391243624	35.0	32.0	38.0	26.5	40.0
76-77	32.30009059584678	33.5	30.0	36.0	25.5	39.0
78-79	33.32598590644496	35.0	31.5	36.5	26.0	39.0
80-81	33.40765118405291	35.0	32.0	36.0	27.0	39.0
82-83	33.22682516220263	35.0	32.0	36.0	27.0	37.5
84-85	33.02732696747891	35.0	32.0	36.0	27.0	37.0
86-87	32.82853886365851	34.0	32.0	35.0	27.0	37.0
88-89	32.659365863740106	34.0	32.0	35.0	26.0	36.0
90-91	32.55418316024459	34.0	32.0	35.0	26.5	36.0
92-93	32.500713659541475	34.0	32.0	35.0	27.0	36.0
94-95	32.51743440783166	34.0	32.0	35.0	27.0	35.5
96-97	32.590227154601976	34.0	32.0	35.0	27.0	35.0
98-99	32.71530171160678	34.0	32.0	35.0	28.5	35.0
100	31.6924001783139	34.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	8.0
19	42.0
20	293.0
21	2256.0
22	10295.0
23	33367.0
24	82358.0
25	163650.0
26	273060.0
27	406325.0
28	548238.0
29	689059.0
30	835597.0
31	989936.0
32	1169314.0
33	1396299.0
34	1710279.0
35	2142629.0
36	2830562.0
37	3816378.0
38	3925579.0
39	1701069.0
40	49945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.81555757878754	21.589478541932998	14.67689186667035	23.918072012609116
2	14.483840083628447	22.467497128942174	41.07611849068483	21.972544296744555
3	17.65860942033452	27.63630750199021	31.268662863099873	23.4364202145754
4	12.392010509769666	19.35990929607251	39.63487050393383	28.613209690223997
5	12.455658868979173	38.692239413547426	36.372729851537144	12.47937186593626
6	28.554575390053778	39.775911520183115	19.191142253877995	12.478370835885118
7	25.07424415974701	33.26068547991422	24.56965037576605	17.09541998457272
8	23.637528950293984	37.63752693067195	21.732283381597178	16.992660737436886
9	25.32994640565	16.357190401484065	21.686165533858468	36.62669765900747
10-11	23.607497828225142	27.179038985345382	29.846915008415326	19.36654817801415
12-13	24.454856764569694	24.825858142513344	29.7686319576369	20.950653135280067
14-15	21.647479757709153	26.11149104688359	27.2174659941873	25.023563201219957
16-17	21.218866149275645	29.125930703439145	27.91662267020921	21.738580477076
18-19	21.237465466211983	28.4758334884462	29.4655059021443	20.821195143197514
20-21	22.065133389186858	27.708889031052276	29.21118669919759	21.014790880563275
22-23	21.812341518087454	27.629542388785143	29.071264071309855	21.48685202181755
24-25	21.617495771387052	27.783501881605705	29.0816076205372	21.517394726470048
26-27	21.61318464091065	27.90359165175876	28.96621392250601	21.517009784824584
28-29	21.56609742139553	28.002025881567395	28.97639467906742	21.455482017969658
30-31	21.80372650341836	27.81819285078624	28.9085727771407	21.469507868654702
32-33	21.633746108473034	27.97419691383648	28.84931167764745	21.542745300043038
34-35	21.745657705368547	28.032481000962722	28.84745134611914	21.374409947549587
36-37	21.756071935809153	28.014485997728823	28.72829291010337	21.501149156358657
38-39	21.653638417525265	28.04011091093616	28.799066106419403	21.507184565119168
40-41	21.852947787009132	27.909995041261325	28.699070607041605	21.537986564687934
42-43	21.753113163104423	28.053343808120772	28.466280987534642	21.727262041240163
44-45	21.828717752774896	28.167892157704312	28.279375972647813	21.72401411687298
46-47	21.85580782454459	28.167114625244423	28.089271252935603	21.887806297275386
48-49	21.8369836271276	28.265957178641415	28.03360047278455	21.863458721446438
50-51	21.853533588378323	28.352433765445845	27.889711554815054	21.90432109136078
52-53	21.889975823804015	28.264730330847183	27.876158474859086	21.96913537048972
54-55	21.89958978812656	28.199822900669297	27.93523820347883	21.965349107725316
56-57	21.95107302112726	28.21704565853253	27.916403687254775	21.91547763308543
58-59	21.924093739739988	28.2509208562697	27.897678801818447	21.92730660217186
60-61	21.9753706758944	28.26823273753225	27.90554595562148	21.850850630951864
62-63	21.87190375202624	28.31933603315506	27.882704345944997	21.926055868873707
64-65	21.84359518488422	28.30186509186391	27.844325856478157	22.01021386677371
66-67	21.922871485167995	28.320582076853672	27.76464555635349	21.991900881624836
68-69	21.854769455262257	28.34232941545413	27.79570110624354	22.007200023040074
70-71	21.867955819792684	28.154185668487248	27.851754538772	22.12610397294807
72-73	21.905966321456074	28.17374795290059	27.83200416813377	22.08828155750956
74-75	21.893077779591394	28.2422067853757	27.802426238490487	22.062289196542423
76-77	21.905448228610076	28.22225099978177	27.76579378391839	22.106506987689762
78-79	21.87454847513924	28.214441181775264	27.787489448648344	22.123520894437153
80-81	21.879035315029856	28.237530758048813	27.78379200114582	22.09964192577551
82-83	21.901679265763594	28.22030836411266	27.88021395606462	21.997798414059126
84-85	21.963491630221323	28.279149554853987	27.80619206736774	21.951166747556947
86-87	21.81108078897843	28.31527946729197	27.88612440059805	21.987515343131548
88-89	21.854499394818607	28.23198772369181	27.872894709204616	22.04061817228497
90-91	21.84531277555535	28.20162549110128	27.978361420565744	21.974700312777628
92-93	21.824933681552274	28.224568701947305	27.979274752309795	21.971222864190626
94-95	21.614340660033857	28.308137879477908	28.091167463918353	21.986353996569886
96-97	21.670840987141602	28.434716076990657	28.18990176503474	21.704541170833
98-99	22.696560187889443	30.439214721958802	28.418320519310246	18.445904570841506
100	21.964526541485537	31.199157963814066	22.174584464931613	24.66173102976878
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5081.0
1	4633.0
2	6772.5
3	9900.5
4	13123.5
5	21336.0
6	28584.0
7	33068.0
8	35217.5
9	33502.0
10	29904.5
11	26366.0
12	27974.0
13	36789.0
14	58300.5
15	88484.0
16	112916.5
17	127253.0
18	132158.5
19	129479.5
20	122740.5
21	115531.0
22	113921.0
23	123313.0
24	144963.5
25	179211.5
26	224082.0
27	272131.5
28	322580.5
29	379811.5
30	436488.0
31	490453.5
32	546922.5
33	600742.5
34	651645.5
35	701813.0
36	751366.5
37	798662.0
38	833226.0
39	856977.5
40	881113.5
41	901968.0
42	918293.0
43	933490.0
44	943202.5
45	954266.5
46	958856.5
47	955375.5
48	947072.5
49	921733.5
50	891224.0
51	859195.0
52	816519.0
53	765683.5
54	711745.5
55	652971.0
56	591349.0
57	528038.0
58	462670.0
59	398489.5
60	331895.0
61	267341.0
62	211702.0
63	164723.0
64	125194.0
65	94966.5
66	73000.0
67	56416.0
68	43538.0
69	32639.0
70	24200.0
71	17842.0
72	13016.0
73	9283.0
74	6643.0
75	4752.0
76	3293.0
77	2246.5
78	1536.0
79	1075.5
80	759.5
81	522.0
82	396.5
83	284.0
84	198.5
85	143.5
86	100.5
87	72.5
88	49.5
89	34.0
90	24.0
91	18.5
92	15.0
93	9.5
94	3.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.10744389215587144
3	1.0098110165025512E-4
4	7.463820556757987E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.01014201499182997
10-11	0.0010405443952656722
12-13	9.154156388729648E-4
14-15	1.2512875639270743E-4
16-17	3.6880054515745343E-4
18-19	4.983197842306068E-4
20-21	1.1855065761806087E-4
22-23	0.0
24-25	0.16534161712246281
26-27	0.0016900643140898158
28-29	0.0016979567619709415
30-31	0.0025240199815165093
32-33	0.002139253668924908
34-35	0.0014062049557674356
36-37	3.558435516033198E-4
38-39	4.4293666021418313E-4
40-41	7.590997761421518E-4
42-43	9.258391915354335E-4
44-45	1.0758543960635266E-4
46-47	0.0
48-49	2.3340468748034294E-6
50-51	0.0
52-53	0.0
54-55	2.1046764667418546E-4
56-57	0.0
58-59	9.735937284402234E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.004054888548290766
70-71	0.006492584766365748
72-73	2.62041295402192E-6
74-75	2.863129145125044E-4
76-77	0.0015529984873687446
78-79	0.009954432628269741
80-81	9.618107015290618E-5
82-83	7.521926415501187E-5
84-85	1.920463821384664E-4
86-87	4.8699236238419434E-5
88-89	4.656022785178704E-5
90-91	0.0
92-93	2.115227757299855E-5
94-95	2.4737595950950295E-5
96-97	6.389265624837273E-6
98-99	3.5289967962002587E-6
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	851.0
20-21	3297.0
22-23	6753.0
24-25	11460.0
26-27	15687.0
28-29	24548.0
30-31	51153.0
32-33	64099.0
34-35	89403.0
36-37	122276.0
38-39	150537.0
40-41	159367.0
42-43	182824.0
44-45	218365.0
46-47	204709.0
48-49	192506.0
50-51	168943.0
52-53	161261.0
54-55	174547.0
56-57	184350.0
58-59	195445.0
60-61	198315.0
62-63	192512.0
64-65	200679.0
66-67	215181.0
68-69	223707.0
70-71	227578.0
72-73	220586.0
74-75	224431.0
76-77	206003.0
78-79	229655.0
80-81	246809.0
82-83	243950.0
84-85	245876.0
86-87	265152.0
88-89	293560.0
90-91	326104.0
92-93	360193.0
94-95	451942.0
96-97	982198.0
98-99	1539586.0
100-101	1.3300141E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.91245059048266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.74156226244602	73.57780396407297
2	7.435933425955279	12.330629255472713
3	2.059297838580822	5.122242908772605
4	0.7816571804423088	2.592364494084757
5	0.3855786480392477	1.5984635302149615
6	0.20113967885381231	1.0006190210851358
7	0.12126855806794694	0.7038271330291411
8	0.07272939683638385	0.4824138017337826
9	0.04479188426219233	0.33424244016692756
>10	0.15158377408337956	1.8806578577702473
>50	0.0031762514451795887	0.17713101484360674
>100	0.0012314485673728247	0.1650268760686936
>500	2.98272017153293E-5	0.017425107068660305
>1k	1.982521834165032E-5	0.017152595615825557
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.390482680445874E-6
2	0.0	8.780965360891749E-6	0.0	0.0	4.390482680445874E-6
3	0.0	8.780965360891749E-6	0.0	0.0	4.390482680445874E-6
4	0.0	4.3904826804458745E-5	0.0	0.0	4.390482680445874E-6
5	0.0	4.3904826804458745E-5	0.0	0.0	4.390482680445874E-6
6	4.829530948490462E-5	4.3904826804458745E-5	0.0	0.0	8.780965360891749E-6
7	4.829530948490462E-5	4.3904826804458745E-5	0.0	0.0	8.780965360891749E-6
8	4.829530948490462E-5	4.3904826804458745E-5	0.0	0.0	8.780965360891749E-6
9	4.829530948490462E-5	1.1854303237203861E-4	0.0	0.0	1.3171448041337624E-5
10-11	4.829530948490462E-5	1.1854303237203861E-4	0.0	0.0	1.3171448041337624E-5
12-13	5.2685792165350495E-5	1.1854303237203861E-4	0.0	0.0	1.536668938156056E-5
14-15	5.2685792165350495E-5	1.5586213515582852E-4	0.0	0.0	2.414765474245231E-5
16-17	1.4927641113515974E-4	1.6025261783627443E-4	0.0	0.0	2.6342896082675248E-5
18-19	1.536668938156056E-4	1.7561930721783498E-4	0.0	0.0	3.5123861443566995E-5
20-21	1.536668938156056E-4	1.887907552591726E-4	0.0	0.0	4.610006814468168E-5
22-23	1.536668938156056E-4	2.2171937536251667E-4	0.0	6.585724020668811E-6	5.488103350557343E-5
24-25	1.536668938156056E-4	2.4586703010496896E-4	0.0	3.951434412401287E-5	6.366199886646518E-5
26-27	1.536668938156056E-4	2.590384781463066E-4	0.0	1.0756682567092393E-4	6.585724020668812E-5
28-29	1.5805737649605148E-4	2.590384781463066E-4	0.0	4.610006814468168E-4	6.585724020668812E-5
30-31	1.5805737649605148E-4	2.9635758093009653E-4	0.0	0.001745216865477235	6.585724020668812E-5
32-33	1.712288245373891E-4	3.051385462909883E-4	0.0	0.005547374866743362	6.585724020668812E-5
34-35	1.8000978989828086E-4	3.49043373095447E-4	0.0	0.010223238921418219	6.585724020668812E-5
36-37	1.8000978989828086E-4	3.9294819989990577E-4	0.0	0.018352217604263754	6.585724020668812E-5
38-39	1.8000978989828086E-4	4.039244066010205E-4	0.0	0.04039683114278249	6.585724020668812E-5
40-41	1.887907552591726E-4	4.456339920652563E-4	0.0	0.06174774841779078	6.585724020668812E-5
42-43	1.9757172062006436E-4	4.54414957426148E-4	0.0	0.07519360162665628	7.463820556757987E-5
44-45	2.063526859809561E-4	5.005150255708297E-4	0.0	0.09386412922525236	9.220013628936337E-5
46-47	2.1513365134184786E-4	5.400293696948426E-4	0.0	0.11524358463768354	9.220013628936337E-5
48-49	2.1952413402229372E-4	5.422246110350655E-4	0.0	0.1329547917706022	9.220013628936337E-5
50-51	2.4367178876474603E-4	6.168628166026455E-4	0.0	0.15118846634249392	9.220013628936337E-5
52-53	2.4586703010496896E-4	6.234485406233142E-4	0.0	0.19332612386807319	1.0098110165025512E-4
54-55	2.4586703010496896E-4	6.30034264643983E-4	0.0	0.24336884545979526	1.0976206701114686E-4
56-57	2.4586703010496896E-4	6.366199886646518E-4	0.0	0.2975210588404147	1.0976206701114686E-4
58-59	2.480622714451919E-4	6.410104713450977E-4	0.0	0.3419220101877638	1.0976206701114686E-4
60-61	2.722099261876442E-4	6.475961953657665E-4	0.0	0.3603971613070801	1.0976206701114686E-4
62-63	3.2050523567254887E-4	6.629628847473271E-4	0.0	0.37871645029124046	1.0976206701114686E-4
64-65	3.227004770127718E-4	7.156486769126775E-4	0.0	0.3858202512682019	1.0976206701114686E-4
66-67	3.292862010334406E-4	7.222344009333464E-4	0.0	0.39140494523772906	1.1415254969159274E-4
68-69	3.292862010334406E-4	7.463820556757987E-4	0.0	0.39713672037705117	1.1854303237203861E-4
70-71	3.336766837138865E-4	7.639439863975822E-4	0.0	0.40205625622049074	1.295192390731533E-4
72-73	3.5782433845633877E-4	7.661392277378051E-4	0.0	0.40618330994010987	1.3610496309382212E-4
74-75	3.600195797965617E-4	7.858963997998115E-4	0.0	0.40944982905436156	1.3610496309382212E-4
76-77	3.709957864976764E-4	8.232155025836014E-4	0.0	0.4125890241708804	1.3610496309382212E-4
78-79	3.775815105183452E-4	8.38582191965162E-4	0.0	0.4147227987535771	1.3610496309382212E-4
80-81	3.775815105183452E-4	8.38582191965162E-4	0.0	0.415480157015954	1.40495445774268E-4
82-83	3.819719931987911E-4	8.429726746456079E-4	0.0	0.4159455481800813	1.4927641113515974E-4
84-85	3.885577172194599E-4	8.473631573260538E-4	0.0	0.41615409610740245	1.4927641113515974E-4
86-87	3.9514344124012867E-4	8.517536400064996E-4	0.0	0.4162858105878158	1.4927641113515974E-4
88	4.039244066010205E-4	8.780965360891749E-4	0.0	0.4163055677598778	1.536668938156056E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	26610	0.0	46.05668	1
GTATCAA	46480	0.0	33.46837	1
ATCAACG	55835	0.0	27.06679	3
TCAACGC	56185	0.0	26.883224	4
CAACGCA	57020	0.0	26.497261	5
AACGCAG	58390	0.0	25.976328	6
TATCAAC	59525	0.0	25.603985	2
ACGCAGA	66890	0.0	22.581657	7
CGCAGAG	67695	0.0	22.306921	8
GTACATG	60875	0.0	20.243044	1
TACATGG	61270	0.0	19.645924	2
ACATGGG	62025	0.0	18.90735	3
GCAGAGT	81085	0.0	18.44195	9
AGAGTAC	72705	0.0	18.17373	10-11
GAGTACT	48540	0.0	17.716293	12-13
CATGGGG	43630	0.0	16.982258	4
CAGAGTA	79510	0.0	16.969578	10-11
AGTACTT	53505	0.0	15.7426195	12-13
GTACTTT	55830	0.0	15.396008	14-15
GTGGTAT	13630	0.0	14.466916	1
>>END_MODULE