FastQCFastQC Report
Fri 27 May 2016
SRR2138609_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138609_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24530895
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA312900.050.258211
GTATCAA566600.034.924991
ATCAACG710950.027.1927343
TCAACGC717750.026.9409084
CAACGCA724300.026.6631185
TATCAAC750650.026.0454122
AACGCAG743350.026.0414186
ACGCAGA851800.022.64317
CGCAGAG861400.022.395728
GTACATG749100.021.1931671
TACATGG753700.020.6934052
ACATGGG773000.019.8345323
GCAGAGT1007800.018.9111149
AGAGTAC925500.018.49955210-11
GAGTACT601900.017.96769512-13
CAGAGTA992000.017.43565610-11
CATGGGG502650.017.425284
AGTACTT666800.016.42178312-13
GTGGTAT142100.016.1572781
GTACTTT694050.015.56796314-15
Produced by FastQC (version 0.11.5)