FastQCFastQC Report
Fri 27 May 2016
SRR2138608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24404482
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA294200.045.24651
GTATCAA516450.033.8256871
ATCAACG636000.026.7232823
TCAACGC647250.026.2848364
CAACGCA652450.026.0568925
TATCAAC668800.025.8007182
AACGCAG667200.025.6440776
ACGCAGA762150.022.3012687
CGCAGAG767750.022.1004228
TACATGG671350.020.3961092
GTACATG690400.020.2886371
ACATGGG683400.019.5821173
GCAGAGT900200.018.6161169
AGAGTAC820150.018.23314710-11
GAGTACT543850.017.44495812-13
CATGGGG507400.017.3877984
CAGAGTA884400.016.89433910-11
AGTACTT607600.015.98327412-13
GTACTAG66050.015.6102351
GTACTTT633750.014.90169914-15
Produced by FastQC (version 0.11.5)