FastQCFastQC Report
Fri 27 May 2016
SRR2138607_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138607_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22405652
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA282700.047.120461
GTATCAA496100.033.746071
ATCAACG604250.026.9073243
TCAACGC608700.026.7242724
CAACGCA609850.026.6263475
AACGCAG625650.026.0344626
TATCAAC652550.025.2630672
ACGCAGA719450.022.5703817
CGCAGAG728700.022.3184458
GTACATG631100.020.9811651
TACATGG620250.020.745972
ACATGGG626850.020.0649013
GCAGAGT847600.019.0046279
AGAGTAC778250.018.3098710-11
GAGTACT532000.017.84857412-13
CATGGGG449600.017.8108444
CAGAGTA838950.017.40736210-11
AGTACTT584300.016.02137412-13
GTGGTAT137850.016.0142771
GTACTTT613250.015.460978514-15
Produced by FastQC (version 0.11.5)