FastQCFastQC Report
Fri 27 May 2016
SRR2138606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5932879
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58900.048.1915781
GTATCAA106550.035.9301531
ATCAACG135850.027.1209053
TCAACGC138250.026.6815854
CAACGCA139300.026.4804695
TATCAAC148500.025.4098642
AACGCAG144300.025.299086
ACGCAGA164700.022.191187
CGCAGAG165600.021.5085958
GTACATG128550.019.9418071
TACATGG128450.019.1340072
AGAGTAC176450.018.62740110-11
ACATGGG128650.018.4129713
GCAGAGT193200.018.1075089
CAGAGTA193550.017.1349649
GAGTACT130250.016.8877312-13
AGTACTT144600.016.45507412-13
CATGGGG96500.016.2193974
GTGGTAT28950.015.9272891
TATACCG5151.8189894E-1214.7851455
Produced by FastQC (version 0.11.5)