##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138606_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5932879
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.969300570599874	31.0	30.0	31.0	26.0	34.0
2	30.647927422757146	31.0	30.0	33.0	27.0	34.0
3	30.854532512798592	31.0	30.0	34.0	27.0	34.0
4	34.72893160302106	35.0	35.0	37.0	32.0	37.0
5	34.42887458180084	35.0	35.0	37.0	30.0	37.0
6	34.551552964420814	35.0	35.0	37.0	30.0	37.0
7	34.380016683299964	35.0	35.0	37.0	30.0	37.0
8	34.372396268321	35.0	35.0	37.0	30.0	37.0
9	35.73799600497499	37.0	35.0	39.0	30.0	39.0
10-11	35.70893625169163	37.0	35.0	39.0	30.0	39.0
12-13	35.655232055128714	37.0	35.0	39.0	30.0	39.0
14-15	36.450210091930074	38.0	35.0	40.0	30.0	41.0
16-17	36.30295359133399	38.0	35.0	40.0	29.5	41.0
18-19	36.5654153742222	38.5	35.0	40.0	30.0	41.0
20-21	36.507858066903836	38.5	35.0	40.0	30.0	41.0
22-23	36.350718265901804	38.0	34.5	40.0	29.5	41.0
24-25	36.38006344523603	38.0	35.0	40.0	30.0	41.0
26-27	36.10734205102893	38.0	34.0	40.0	29.0	41.0
28-29	35.789865553936565	38.0	34.0	40.0	27.0	41.0
30-31	35.854018586011094	38.0	34.0	40.0	27.0	41.0
32-33	35.91971996032402	38.0	34.0	40.0	28.5	41.0
34-35	35.629320216359446	38.0	34.0	40.0	27.0	41.0
36-37	35.45402666282931	38.0	34.0	40.0	26.5	41.0
38-39	35.575961093727564	38.0	34.0	40.0	27.0	41.0
40-41	35.565223262716145	38.0	34.0	40.0	27.0	41.0
42-43	35.55484209166321	38.0	34.0	40.0	27.0	41.0
44-45	35.70112024838596	38.0	34.0	40.0	27.5	41.0
46-47	35.46077995588335	38.0	33.5	40.0	27.0	41.0
48-49	35.293423321459144	38.0	33.0	40.0	26.5	41.0
50-51	35.22445279295974	38.0	33.0	40.0	26.5	41.0
52-53	35.322979631381855	38.0	33.0	40.0	27.0	41.0
54-55	35.35126115693065	38.0	33.0	40.0	27.0	41.0
56-57	35.267197085310556	37.5	33.0	40.0	27.5	41.0
58-59	34.86709616575307	37.0	33.0	40.0	26.0	41.0
60-61	34.362786653623225	37.0	32.0	40.0	25.5	41.0
62-63	34.319153244028776	36.0	32.0	39.0	25.5	41.0
64-65	34.24674285068872	36.0	32.0	39.0	26.0	41.0
66-67	33.95881172140665	36.0	31.5	39.0	26.0	40.0
68-69	33.70271192082946	35.0	31.0	39.0	25.5	40.0
70-71	33.75584012903312	35.0	31.5	38.5	26.0	40.0
72-73	33.54667328509763	35.0	31.0	38.0	26.0	40.0
74-75	33.08736309126168	35.0	31.0	37.0	25.5	39.5
76-77	32.712686601207075	34.5	31.0	37.0	24.5	39.0
78-79	32.624135516503806	34.0	31.0	36.0	25.5	39.0
80-81	32.48320048698415	34.0	31.0	36.0	25.5	38.5
82-83	32.57957746678997	34.0	31.0	36.0	26.0	37.0
84-85	32.16998579634001	34.0	31.0	35.0	25.5	37.0
86-87	32.01877632958111	34.0	31.0	35.0	25.5	36.5
88-89	32.06043204333718	34.0	31.0	35.0	26.0	36.0
90-91	31.86181463196941	34.0	31.0	35.0	25.0	36.0
92-93	31.755352733246074	34.0	31.0	35.0	25.0	36.0
94-95	31.97036346916671	34.0	31.0	35.0	26.0	35.0
96-97	32.14417522462334	34.0	31.0	35.0	26.5	35.0
98-99	32.67809155030701	34.0	32.0	35.0	28.5	35.0
100	32.50628188992249	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	33.0
20	176.0
21	1183.0
22	4886.0
23	14449.0
24	34086.0
25	63961.0
26	104546.0
27	151256.0
28	202048.0
29	251268.0
30	302134.0
31	352838.0
32	405194.0
33	463516.0
34	530875.0
35	600155.0
36	685339.0
37	773026.0
38	702034.0
39	282124.0
40	7747.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.861311346481195	21.935454945229797	14.764147389488308	26.439086318800705
2	14.301363924142382	23.23535698079826	39.00415805041577	23.459121044643584
3	16.79278879005352	27.34976336932151	31.29483282469845	24.562615015926514
4	12.150723132921447	20.752351519881206	38.81445136540272	28.28247398179463
5	12.468741972695872	39.31111993576134	35.69317814833817	12.526959943204611
6	27.910497144632245	40.22713493540469	19.24294198026888	12.619425939694187
7	24.248564651326955	33.89201094443355	24.52536112737172	17.334063276867774
8	23.317987776255002	36.95575116229406	21.740659130246883	17.985601931204055
9	24.16765087157546	17.212087828552658	22.482144834622392	36.138116465249496
10-11	23.07744624417581	27.435675808332626	30.067334153744298	19.41954379374727
12-13	23.8441994181914	24.93517902522536	29.851982486074636	21.368639070508603
14-15	21.046695879015903	26.544094359584953	27.376042895868935	25.03316686553021
16-17	20.556436428250098	29.226325027023137	28.21480094234182	22.002437602384948
18-19	20.68601938451804	28.611792015309934	29.806203699755212	20.895984900416813
20-21	21.39413039973612	27.832852614800778	29.51924886492641	21.253768120536687
22-23	21.015573586261564	27.772235099558284	29.57570943424159	21.636481879938565
24-25	20.959884148277723	27.8319930738618	29.5378961897384	21.670226588122077
26-27	21.14071687548267	27.91871624672154	29.293025310167998	21.647541567627783
28-29	20.925118340517663	27.878513678420543	29.47249920757041	21.723868773491386
30-31	21.48325084700232	27.837281102024264	29.222504723247688	21.456963327725724
32-33	21.298542860615893	28.016632420835975	29.19039225677494	21.494432461773197
34-35	21.256434232725695	27.889393005227696	29.286443452470838	21.567729309575764
36-37	21.290583002002123	27.87712375896529	29.22915666822371	21.603136570808875
38-39	21.232024254367264	27.951557890800053	29.218933064038787	21.597484790793892
40-41	21.42542143490659	27.829687438315194	29.102987071426394	21.641904055351823
42-43	21.327164953894528	28.007845764082095	28.94600563869733	21.718983643326048
44-45	21.51745367949264	28.198712499333052	28.56730702931698	21.71652679185733
46-47	21.456616122417632	28.027807438236085	28.446716609359772	22.068859829986508
48-49	21.499959626899074	28.102859966300493	28.52715184617367	21.87002856062676
50-51	21.424949665838653	28.255521038848585	28.382269261153347	21.937260034159415
52-53	21.57979931415749	28.24245166077035	28.268320047773727	21.909428977298433
54-55	21.564542137363805	28.123564892169224	28.349973519725296	21.961919450741675
56-57	21.65299326977969	28.219945073639895	28.319501283572198	21.807560373008222
58-59	21.50598400126925	28.25578100062221	28.39493907941778	21.843295918690757
60-61	21.50149933939303	28.177893556721035	28.346257168263563	21.974349935622378
62-63	21.523168531934427	28.2905593746955	28.306953394104394	21.879318699265674
64-65	21.39531400027809	28.26369386149435	28.46919612194308	21.87179601628448
66-67	21.50843874360091	28.2683841416041	28.30642384002929	21.916753274765707
68-69	21.383410942956928	28.261263096623978	28.434090027163368	21.921235933255723
70-71	21.454084696205854	28.02308693382481	28.46398364798063	22.05884472198871
72-73	21.461408916497657	28.154390493022085	28.466485350358354	21.9177152401219
74-75	21.214457619935377	28.272741074497294	28.553356732492492	21.959444573074837
76-77	21.393907171926237	28.26306274860975	28.41892753446548	21.92410254499853
78-79	21.41838769517658	28.28964179345077	28.334940057891245	21.95703045348141
80-81	21.397346522528125	28.317928428795184	28.350734033179307	21.93399101549738
82-83	21.480606114316995	28.25990078634814	28.446156351438823	21.813336747896045
84-85	21.498884805913594	28.309312470188207	28.425591106160613	21.766211617737582
86-87	21.371301589305997	28.3044735868505	28.400079312858306	21.9241455109852
88-89	21.405506188468014	28.3017344800102	28.37399212447689	21.918767207044894
90-91	21.309404102911934	28.237925764135912	28.538041343062282	21.91462878988987
92-93	21.307858400428987	28.287888746946933	28.546981994818132	21.857270857805947
94-95	21.109859333117576	28.43188527141518	28.54998396990193	21.908271425565314
96-97	21.140414610078597	28.549206236972335	28.614694783210787	21.69568436973828
98-99	22.15384262458823	30.639321624702404	28.574547531386514	18.632288219322852
100	21.365574235403585	31.492008915701618	22.382477505666067	24.75993934322873
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	463.0
1	476.5
2	1217.5
3	2039.0
4	2548.5
5	4269.0
6	6141.0
7	7437.5
8	8219.5
9	8036.0
10	7075.5
11	5967.5
12	5987.0
13	7636.5
14	12569.0
15	20089.5
16	26348.0
17	30029.5
18	31596.5
19	31638.5
20	30946.5
21	30554.0
22	31402.0
23	34641.0
24	41069.5
25	51147.5
26	64338.5
27	78453.5
28	92803.5
29	107680.0
30	121922.5
31	136024.5
32	150881.0
33	164847.0
34	178437.0
35	191596.0
36	204772.5
37	217435.0
38	224772.0
39	228734.5
40	233465.5
41	237709.5
42	240084.5
43	242330.5
44	243930.0
45	245313.0
46	245507.0
47	243127.0
48	238759.0
49	230374.0
50	220726.0
51	211491.5
52	200106.0
53	186679.5
54	172320.5
55	157355.5
56	143367.0
57	128434.0
58	112792.5
59	98009.5
60	81746.0
61	65748.0
62	52125.0
63	40498.5
64	30579.5
65	22585.0
66	16916.5
67	13020.5
68	10053.5
69	7548.5
70	5643.5
71	4200.0
72	3005.0
73	2151.5
74	1547.0
75	1107.0
76	785.5
77	543.5
78	361.5
79	241.0
80	166.0
81	114.0
82	79.0
83	56.0
84	40.5
85	27.5
86	22.0
87	18.0
88	16.0
89	12.0
90	5.5
91	2.0
92	1.0
93	1.5
94	2.0
95	2.5
96	3.0
97	2.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004904869962795466
3	8.427611619923481E-5
4	2.3597312535785745E-4
5	5.056566971954088E-5
6	3.033940183172453E-4
7	0.0
8	0.0
9	3.371044647969392E-5
10-11	0.001348417859187757
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0376922879825658
22-23	0.0
24-25	1.433806388231587E-4
26-27	0.005073392403603645
28-29	0.0
30-31	2.539038564271868E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	6.868585921287722E-5
40-41	0.0022200637788906312
42-43	1.304205724367597E-4
44-45	8.147895696421672E-4
46-47	0.002324273233784328
48-49	0.007993769671958686
50-51	0.006852970461811171
52-53	0.009029142779698506
54-55	0.010937299198022537
56-57	0.012154762930674119
58-59	0.013917577548334394
60-61	0.015407113337081649
62-63	0.01317573506831765
64-65	0.03783509795646709
66-67	0.005867430450542795
68-69	0.004530256535719618
70-71	0.0030585575548479262
72-73	9.611363048827408E-4
74-75	2.1030357320792566E-4
76-77	8.098443054076746E-5
78-79	0.0
80-81	0.0
82-83	7.358965943336383E-5
84-85	6.395374183217451E-5
86-87	5.405726242562937E-5
88-89	1.0983072888064916E-5
90-91	4.478499451887648E-5
92-93	0.003209295398624183
94-95	2.334196480288469E-5
96-97	3.4872589387164747E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	369.0
20-21	1278.0
22-23	2165.0
24-25	4623.0
26-27	5647.0
28-29	7907.0
30-31	12883.0
32-33	15922.0
34-35	21689.0
36-37	28284.0
38-39	35044.0
40-41	36545.0
42-43	41648.0
44-45	48801.0
46-47	48099.0
48-49	44682.0
50-51	40725.0
52-53	40275.0
54-55	42809.0
56-57	45975.0
58-59	47421.0
60-61	47398.0
62-63	45233.0
64-65	47994.0
66-67	52020.0
68-69	53332.0
70-71	54737.0
72-73	53479.0
74-75	53249.0
76-77	55413.0
78-79	62516.0
80-81	62373.0
82-83	65598.0
84-85	65907.0
86-87	68661.0
88-89	85763.0
90-91	86406.0
92-93	90777.0
94-95	110222.0
96-97	244229.0
98-99	541477.0
100-101	3413304.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.2694399029393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.5971018783146	85.42555064972123
2	4.7572290068370435	8.683792538880665
3	1.0346845161492564	2.8330522879555917
4	0.3165256448091638	1.1555647326659635
5	0.13515427160878732	0.6167727335111876
6	0.06277407969882402	0.3437613056720485
7	0.03276224264775791	0.20931340754975386
8	0.017795296501367477	0.1299333395669236
9	0.012418879950216226	0.10201177955502701
>10	0.03308366867662925	0.43358498360052766
>50	3.042102586051394E-4	0.01917561938332554
>100	1.663045477714672E-4	0.04748662193776414
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.685522323984696E-5	3.371044647969392E-5
2	0.0	1.685522323984696E-5	0.0	3.371044647969392E-5	3.371044647969392E-5
3	0.0	1.685522323984696E-5	0.0	3.371044647969392E-5	3.371044647969392E-5
4	0.0	5.0565669719540887E-5	0.0	5.0565669719540887E-5	5.0565669719540887E-5
5	0.0	5.0565669719540887E-5	0.0	5.0565669719540887E-5	6.742089295938784E-5
6	0.0	5.0565669719540887E-5	0.0	6.742089295938784E-5	8.427611619923481E-5
7	0.0	5.0565669719540887E-5	0.0	6.742089295938784E-5	1.0113133943908177E-4
8	1.685522323984696E-5	6.742089295938784E-5	0.0	8.427611619923481E-5	1.0113133943908177E-4
9	1.685522323984696E-5	2.0226267887816355E-4	0.0	1.6855223239846962E-4	1.0113133943908177E-4
10-11	3.371044647969392E-5	2.0226267887816355E-4	0.0	1.8540745563831658E-4	1.0113133943908177E-4
12-13	3.371044647969392E-5	2.0226267887816355E-4	0.0	1.9383506725824005E-4	1.0113133943908177E-4
14-15	5.0565669719540887E-5	3.033940183172453E-4	0.0	2.0226267887816355E-4	1.0955895105900526E-4
16-17	5.899328133946436E-5	3.033940183172453E-4	0.0	2.0226267887816355E-4	1.1798656267892872E-4
18-19	6.742089295938784E-5	3.118216299371688E-4	0.0	2.0226267887816355E-4	1.1798656267892872E-4
20-21	9.270372781915829E-5	3.2024924155709224E-4	0.0	2.191179021180105E-4	1.348417859187757E-4
22-23	1.1798656267892872E-4	3.6238729965670964E-4	0.0	2.3597312535785745E-4	1.4326939753869918E-4
24-25	1.264141742988522E-4	3.960977461364036E-4	0.0	2.5282834859770444E-4	1.5169700915862265E-4
26-27	1.348417859187757E-4	4.045253577563271E-4	0.0	2.9496640669732184E-4	1.5169700915862265E-4
28-29	1.348417859187757E-4	4.1295296937625056E-4	0.0	5.309395320551792E-4	1.6855223239846962E-4
30-31	1.5169700915862265E-4	4.3823580423602097E-4	0.0	0.0013399902475678335	1.6855223239846962E-4
32-33	2.1911790211801048E-4	4.3823580423602097E-4	0.0	0.0035648797152276323	1.6855223239846962E-4
34-35	2.612559602176279E-4	5.477947552950263E-4	0.0	0.006169011705783988	1.8540745563831658E-4
36-37	2.8653879507739837E-4	6.404984831141845E-4	0.0	0.010669356310823126	1.8540745563831658E-4
38-39	3.033940183172453E-4	6.404984831141845E-4	0.0	0.01891156047510829	1.8540745563831658E-4
40-41	3.2024924155709224E-4	7.500574341731897E-4	0.0	0.02873815562393907	1.8540745563831658E-4
42-43	3.2024924155709224E-4	8.259059387525011E-4	0.0	0.03644099264454913	1.8540745563831658E-4
44-45	3.371044647969392E-4	9.354648898115064E-4	0.0	0.046385574356058834	1.8540745563831658E-4
46-47	3.5395968803678617E-4	9.944581711509707E-4	0.0	0.056422859795387706	2.0226267887816355E-4
48-49	3.5395968803678617E-4	9.944581711509707E-4	0.0	0.0663337310604177	2.0226267887816355E-4
50-51	3.5395968803678617E-4	0.0012220036848889049	0.0	0.07649743067404544	2.0226267887816355E-4
52-53	3.6238729965670964E-4	0.0012304312965088283	0.0	0.08677068923873216	2.1069029049808702E-4
54-55	3.7081491127663316E-4	0.0012472865197486753	0.0	0.09785299851893153	2.1911790211801048E-4
56-57	3.7081491127663316E-4	0.001280996966228369	0.0	0.1065671489339324	2.1911790211801048E-4
58-59	3.876701345164801E-4	0.001297852189468216	0.0	0.1149610501073762	2.1911790211801048E-4
60-61	4.1295296937625056E-4	0.00133156263594791	0.0	0.12458538257732882	2.1911790211801048E-4
62-63	4.2138058099617403E-4	0.0013737006940475272	0.0	0.13506933143251362	2.1911790211801048E-4
64-65	4.719462507157149E-4	0.0014664044218666856	0.0	0.1441880072052708	2.1911790211801048E-4
66-67	4.719462507157149E-4	0.0014664044218666856	0.0	0.15050028830859352	2.1911790211801048E-4
68-69	5.140843088153324E-4	0.00152539770320615	0.0	0.15731822610911161	2.2754551373793398E-4
70-71	5.393671436751028E-4	0.001559108149685844	0.0	0.16307428484551934	2.5282834859770444E-4
72-73	5.393671436751028E-4	0.0015759633729256909	0.0	0.1686533637379087	2.5282834859770444E-4
74-75	5.562223669149497E-4	0.001668667100744849	0.0	0.1730694322267486	2.5282834859770444E-4
76-77	5.562223669149497E-4	0.0017445156053241605	0.0	0.176878712678954	2.5282834859770444E-4
78-79	5.562223669149497E-4	0.001786653663423778	0.0	0.17878335290505673	2.5282834859770444E-4
80-81	5.562223669149497E-4	0.0017950812750437014	0.0	0.17923844393253258	2.612559602176279E-4
82-83	5.562223669149497E-4	0.0018119364982835482	0.0	0.1797525282413479	2.696835718375514E-4
84-85	5.562223669149497E-4	0.0018203641099034718	0.0	0.18011491554100462	2.696835718375514E-4
86-87	5.562223669149497E-4	0.0018793573912429362	0.0	0.18023290210368356	2.696835718375514E-4
88	5.562223669149497E-4	0.0019552058958222473	0.0	0.18023290210368356	2.696835718375514E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5890	0.0	48.191578	1
GTATCAA	10655	0.0	35.930153	1
ATCAACG	13585	0.0	27.120905	3
TCAACGC	13825	0.0	26.681585	4
CAACGCA	13930	0.0	26.480469	5
TATCAAC	14850	0.0	25.409864	2
AACGCAG	14430	0.0	25.29908	6
ACGCAGA	16470	0.0	22.19118	7
CGCAGAG	16560	0.0	21.508595	8
GTACATG	12855	0.0	19.941807	1
TACATGG	12845	0.0	19.134007	2
AGAGTAC	17645	0.0	18.627401	10-11
ACATGGG	12865	0.0	18.412971	3
GCAGAGT	19320	0.0	18.107508	9
CAGAGTA	19355	0.0	17.134964	9
GAGTACT	13025	0.0	16.88773	12-13
AGTACTT	14460	0.0	16.455074	12-13
CATGGGG	9650	0.0	16.219397	4
GTGGTAT	2895	0.0	15.927289	1
TATACCG	515	1.8189894E-12	14.785145	5
>>END_MODULE