FastQCFastQC Report
Fri 27 May 2016
SRR2138605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9853934
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124650.043.883511
GTATCAA211950.032.899211
ATCAACG246150.027.1508643
CAACGCA259300.025.709065
ACGCAGA260550.025.6179457
AACGCAG263450.025.3359496
TCAACGC264400.025.2925454
TATCAAC275700.024.8045522
CGCAGAG305850.021.8236248
GTACATG278000.020.3862921
ACATGGG266400.020.0286543
TACATGG277750.019.84532
GCAGAGT357900.018.4269189
AGAGTAC329600.018.18139310-11
GAGTACT205800.018.01174412-13
CATGGGG206550.017.1739334
CAGAGTA357700.016.7765810-11
AGTACTT236000.015.76911112-13
GTACTTT240800.015.33359614-15
ATGGGGG137050.015.3154315
Produced by FastQC (version 0.11.5)