##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138605_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9853934
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15914689503705	33.0	31.0	34.0	30.0	34.0
2	32.302860969030235	34.0	31.0	34.0	30.0	34.0
3	33.05091956166948	34.0	31.0	34.0	30.0	37.0
4	35.86600021879586	37.0	35.0	37.0	35.0	37.0
5	35.77672643230612	37.0	35.0	37.0	35.0	37.0
6	35.78107281822671	37.0	35.0	37.0	33.0	37.0
7	35.74731919251742	37.0	35.0	37.0	33.0	37.0
8	36.013813975210304	37.0	35.0	37.0	33.0	39.0
9	37.23973318676581	39.0	37.0	39.0	33.0	39.0
10-11	37.178914482276824	39.0	37.0	39.0	33.0	39.0
12-13	37.29542454820583	39.0	37.0	39.0	33.0	39.5
14-15	38.32084099609354	40.0	38.0	41.0	33.0	41.0
16-17	38.321704356858895	40.0	38.0	41.0	33.0	41.0
18-19	38.36266799635557	40.0	38.0	41.0	33.5	41.0
20-21	38.33586879648313	40.0	38.0	41.0	34.0	41.0
22-23	38.254578062480334	40.0	38.0	41.0	33.0	41.0
24-25	38.164198235796256	40.0	37.5	41.0	33.0	41.0
26-27	37.99858712424864	40.0	37.0	41.0	33.0	41.0
28-29	37.907959990581375	40.0	37.0	41.0	33.0	41.0
30-31	37.80953834400094	40.0	37.0	41.0	33.0	41.0
32-33	37.95759416113694	40.0	37.0	41.0	33.0	41.0
34-35	37.94046985069055	40.0	37.0	41.0	33.0	41.0
36-37	37.840737195671274	40.0	37.0	41.0	33.0	41.0
38-39	37.69693301223403	40.0	37.0	41.0	32.5	41.0
40-41	37.712578713641136	40.0	37.0	41.0	33.0	41.0
42-43	37.683043639652794	40.0	37.0	41.0	32.5	41.0
44-45	37.67576975244495	40.0	37.0	41.0	32.5	41.0
46-47	37.68430125646917	40.0	37.0	41.0	32.5	41.0
48-49	37.60723010654559	40.0	36.5	41.0	32.0	41.0
50-51	37.51412875441611	40.0	36.0	41.0	32.0	41.0
52-53	37.386183449110064	39.0	36.0	41.0	31.5	41.0
54-55	37.2782600235304	39.0	36.0	41.0	31.0	41.0
56-57	37.13556601315999	39.0	35.5	41.0	31.0	41.0
58-59	36.99196900495198	39.0	35.0	40.0	31.0	41.0
60-61	36.78875908752926	38.5	35.0	40.0	31.0	41.0
62-63	36.561623402989284	38.0	35.0	40.0	31.0	41.0
64-65	36.35713964046781	38.0	35.0	40.0	30.5	41.0
66-67	36.08039253776026	37.0	34.5	40.0	30.0	41.0
68-69	35.81229118091497	37.0	34.0	40.0	30.0	41.0
70-71	35.54447894830459	36.5	34.0	39.0	30.0	41.0
72-73	35.252182567162905	36.0	34.0	39.0	29.0	40.0
74-75	34.81444877316892	35.5	33.5	38.5	29.0	40.0
76-77	33.98570223653681	35.0	32.5	37.0	28.5	39.0
78-79	34.25735194132659	35.0	33.0	37.0	29.0	39.0
80-81	34.130793333641066	35.0	33.0	37.0	29.0	39.0
82-83	33.90775088696867	35.0	33.0	36.0	29.0	38.5
84-85	33.6866499775238	35.0	33.0	36.0	29.0	37.0
86-87	33.474154861480244	35.0	33.0	35.5	29.0	37.0
88-89	33.26893409376926	35.0	33.0	35.0	29.0	36.5
90-91	33.161423946454065	35.0	33.0	35.0	29.0	36.0
92-93	33.08817903217959	35.0	33.0	35.0	29.0	36.0
94-95	33.03319595030193	34.0	33.0	35.0	29.0	36.0
96-97	32.977773582155834	34.0	32.5	35.0	29.0	35.0
98-99	33.082711131717915	34.0	33.0	35.0	29.0	35.0
100-101	32.509122374435364	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	14.0
20	80.0
21	442.0
22	2101.0
23	7117.0
24	17885.0
25	37186.0
26	65411.0
27	100309.0
28	141617.0
29	185238.0
30	232997.0
31	287406.0
32	353607.0
33	445306.0
34	589802.0
35	798384.0
36	1196860.0
37	1929952.0
38	2276816.0
39	1126162.0
40	59239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77531097433008	0.010341116808140722	27.524906970541924	30.689440938319855
2	13.896385427479958	26.217288404370002	37.68057360977336	22.205752558376677
3	15.140255222054066	26.181891507438305	34.63342388604586	24.044429384461772
4	12.33746846690875	25.176218959859074	38.22382004994147	24.2624925232907
5	16.276382610234656	38.01435041070906	32.90555832827782	12.80370865077846
6	26.378946723207196	38.811433078403	21.340674699059278	13.46894549933052
7	24.491751213271776	33.73750016998287	23.927093483678703	17.84365513306665
8	23.541978259647365	32.550583350771376	22.916339809054943	20.991098580526316
9	23.19405630279237	20.702909112238828	24.678793261655702	31.424241323313108
10-11	24.2393951491861	26.339490400483704	29.674985645327034	19.74612880500316
12-13	24.255906321272295	24.631208205778524	29.414947370258414	21.697938102690763
14-15	21.774487315141712	27.10315631823576	27.0887356810354	24.03362068558713
16-17	20.956368386105325	29.13386462035007	28.294869672850425	21.614897320694183
18-19	20.987011097065363	28.522155897178497	29.701819424702563	20.789013581053574
20-21	21.867019943471583	27.901120586866835	29.35952408511506	20.872335384546528
22-23	21.6568275922549	27.65116043967376	29.181638766677214	21.510373201394124
24-25	21.41473108760321	27.992504249154017	29.306286169081737	21.28647849416104
26-27	21.40651049698075	28.17499281807983	29.04633648931489	21.372160195624534
28-29	21.313069758669997	28.10193747741344	29.087820495326515	21.49717226859004
30-31	21.615305523729045	28.02420654867868	29.181864804443858	21.178623123148416
32-33	21.55787710243878	28.190021542807848	28.922772885343594	21.329328469409774
34-35	21.512778823867997	28.17037319663724	29.085142077792405	21.231705901702362
36-37	21.603419518730703	28.254403205011698	28.929583857069662	21.212593419187936
38-39	21.365337713277693	28.335401366077328	29.094695769222977	21.204565151422
40-41	21.6016467072899	27.984792640435334	28.917476252525038	21.496084399749726
42-43	21.59042067704205	28.096333350545134	28.79967895613631	21.513567016276507
44-45	21.54385386576654	28.490024014874855	28.487460885747378	21.47866123361123
46-47	21.67898862074463	28.244780197814435	28.219983814783028	21.856247366657904
48-49	21.628951033924864	28.393351808058053	28.319148061234777	21.658549096782306
50-51	21.647307424804534	28.533287294343957	27.97351642146638	21.84588885938513
52-53	21.71075060455701	28.35110167156259	28.019147322903216	21.919000400977183
54-55	21.58948087020786	28.393056910014813	28.159091015428917	21.85837120434841
56-57	21.84712988829173	28.5609951475699	27.866016450784343	21.725858513354027
58-59	21.77709491345521	28.428524061665573	28.067555600214284	21.726825424664934
60-61	21.827329399998842	28.439642188847046	27.98852641161748	21.74450199953663
62-63	21.8177921273329	28.582149216383634	27.882169140014362	21.717889516269107
64-65	21.70691206728656	28.35566473891828	27.911806610956173	22.025616582838982
66-67	21.79380560772485	28.462274486369587	27.863780044770742	21.880139861134825
68-69	21.736224810507228	28.407316783272226	28.00732412346736	21.84913428275319
70-71	21.789583651694866	28.07552411461913	28.055387684539944	22.07950454914606
72-73	21.797704642366035	28.311284003677585	27.979736439831154	21.911274914125226
74-75	21.655257019500716	28.374781884748156	28.106752468555456	21.863208627195668
76-77	21.898882790065368	28.22163675584281	27.841080814836243	22.038399639255577
78-79	21.632440043541614	28.293525333375435	28.032476814166568	22.04155780891638
80-81	21.727814455559667	28.295762044293877	28.083415443998057	21.8930080561484
82-83	21.904804903248266	28.073235956717724	28.16503772509591	21.856921414938103
84-85	21.947184672989078	28.19526388231769	28.027209206053733	21.830342238639496
86-87	21.703022051294536	28.374779068089328	28.027024406108257	21.89517447450788
88-89	21.84089439548487	28.026365111802846	28.08565827772608	22.047082214986204
90-91	21.840955226366578	28.121277105172755	28.146615171609707	21.89115249685096
92-93	21.810491449560207	28.111634636914467	28.116030858934142	21.96184305459118
94-95	21.73673730741545	28.044137107773064	28.188521204665324	22.030604380146162
96-97	21.494046794396407	28.276930189209033	28.356135865405914	21.872887150988646
98-99	21.897294573569802	29.236417758604006	28.2241654961429	20.64212217168329
100-101	22.31734013800421	31.199651090070326	25.13060527229529	21.352403499630178
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1143.0
1	1211.5
2	2532.5
3	4045.5
4	5665.0
5	9049.0
6	11580.5
7	13117.0
8	13826.5
9	13134.5
10	11889.0
11	10829.5
12	12084.5
13	16172.0
14	25814.0
15	40159.5
16	52121.0
17	57756.0
18	57657.0
19	54813.5
20	51177.5
21	48754.5
22	49893.5
23	56399.0
24	67964.0
25	84916.5
26	107565.5
27	131206.5
28	154129.5
29	179676.0
30	205195.0
31	229474.0
32	252897.0
33	274852.0
34	297767.0
35	318682.0
36	339067.5
37	360895.0
38	375438.0
39	381275.5
40	384783.0
41	387214.0
42	389712.0
43	393787.5
44	398358.0
45	403607.5
46	407133.0
47	409025.0
48	407387.0
49	396874.0
50	381920.5
51	366042.5
52	345881.0
53	322373.5
54	300409.5
55	277694.5
56	254784.5
57	228955.0
58	201448.5
59	175117.5
60	144214.5
61	113725.5
62	89844.5
63	69674.0
64	50908.0
65	37022.5
66	27792.5
67	21050.5
68	15922.0
69	11520.0
70	8207.0
71	5929.5
72	4163.0
73	2836.0
74	1936.5
75	1286.0
76	855.0
77	576.5
78	395.5
79	276.0
80	190.0
81	131.0
82	96.5
83	71.5
84	54.5
85	41.5
86	31.5
87	24.0
88	19.0
89	16.0
90	15.5
91	15.0
92	14.5
93	11.5
94	6.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.69783256108677E-4
2	0.001288825356451545
3	5.0741155765808855E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	5.074115576580887E-6
16-17	1.0148231153161773E-5
18-19	2.283352009461399E-4
20-21	0.002334194904970105
22-23	6.698956992696106E-4
24-25	0.0031320998917521914
26-27	0.010522894405395522
28-29	3.9131667286505497E-4
30-31	0.0028550164624015196
32-33	0.0030504868842326964
34-35	0.0037968938133657827
36-37	0.06192769580334335
38-39	0.02564679848420327
40-41	0.02291380787523369
42-43	0.003296774539898833
44-45	0.001820160018067874
46-47	0.004000294498892599
48-49	0.002530101709005147
50-51	8.04144132168473E-4
52-53	0.0013523964935063717
54-55	0.002562173935516989
56-57	0.01343716415878037
58-59	0.008086794548648036
60-61	0.004739170965621308
62-63	0.0028444934032714926
64-65	0.0011435581901051135
66-67	7.232508918217042E-4
68-69	0.0015351806433870113
70-71	2.4275323926888065E-4
72-73	5.343154255573632E-4
74-75	0.0010562746473145553
76-77	0.004281518989888448
78-79	0.0010885921778476966
80-81	7.80822282009261E-4
82-83	9.160463179191092E-4
84-85	0.002831780374807023
86-87	0.01194211142178233
88-89	0.032039722156771575
90-91	3.955804091092591E-4
92-93	5.300882695918681E-4
94-95	0.0018678712585240556
96-97	0.015126136571383364
98-99	1.804927514895753E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	265.0
20-21	951.0
22-23	2024.0
24-25	4218.0
26-27	5761.0
28-29	10927.0
30-31	21704.0
32-33	27965.0
34-35	40899.0
36-37	64617.0
38-39	72720.0
40-41	73001.0
42-43	83292.0
44-45	96575.0
46-47	96854.0
48-49	90881.0
50-51	83290.0
52-53	80502.0
54-55	85175.0
56-57	90852.0
58-59	93494.0
60-61	92613.0
62-63	87497.0
64-65	89455.0
66-67	96422.0
68-69	98034.0
70-71	99199.0
72-73	94100.0
74-75	94386.0
76-77	96354.0
78-79	104473.0
80-81	107918.0
82-83	102970.0
84-85	102046.0
86-87	108360.0
88-89	118050.0
90-91	128881.0
92-93	135404.0
94-95	169698.0
96-97	259452.0
98-99	940509.0
100-101	5602146.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.32830485621024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.94936566522507	71.52772822731734
2	7.65771108095131	12.455773225847745
3	2.2671606159295203	5.531529891909281
4	0.9434417220719344	3.0691406394693694
5	0.45478417772775187	1.8493413125011748
6	0.24030919645877577	1.172636375361014
7	0.14039619998448494	0.7992729467094154
8	0.09344993851751882	0.6080100070837497
9	0.0653274381678591	0.4781672826111782
>10	0.18373827964411776	2.180167250165241
>50	0.0033709397229946406	0.18374640311578253
>100	8.752710211129709E-4	0.11295809301273396
>500	6.947457746034107E-5	0.0315283448959543
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.133408037845596E-5	0.0	0.0	0.0
3	0.0	9.133408037845596E-5	0.0	2.0296462306323547E-5	0.0
4	0.0	9.133408037845596E-5	0.0	2.0296462306323547E-5	0.0
5	0.0	9.133408037845596E-5	0.0	2.0296462306323547E-5	0.0
6	0.0	9.133408037845596E-5	0.0	2.0296462306323547E-5	0.0
7	0.0	9.133408037845596E-5	0.0	2.0296462306323547E-5	0.0
8	0.0	9.133408037845596E-5	0.0	3.044469345948532E-5	0.0
9	0.0	1.9281639191007368E-4	0.0	5.074115576580886E-5	0.0
10-11	0.0	2.0296462306323545E-4	0.0	6.088938691897064E-5	0.0
12-13	0.0	2.0296462306323545E-4	0.0	6.088938691897064E-5	0.0
14-15	0.0	3.0952105017143406E-4	0.0	7.10376180721324E-5	0.0
16-17	0.0	3.348916280543385E-4	0.0	8.625996480187507E-5	0.0
18-19	0.0	3.348916280543385E-4	0.0	9.133408037845596E-5	0.0
20-21	0.0	3.450398592075003E-4	0.0	1.2177877383794127E-4	0.0
22-23	0.0	4.769668641986033E-4	0.0	1.420752361442648E-4	0.0
24-25	0.0	5.175597888112504E-4	0.0	1.5222346729742658E-4	0.0
26-27	0.0	5.37856251117574E-4	0.0	2.0296462306323548E-4	0.0
28-29	0.0	5.480044822707358E-4	0.0	5.074115576580887E-4	0.0
30-31	0.0	5.987456380365446E-4	0.0	0.001476567632785038	0.0
32-33	0.0	5.987456380365446E-4	0.0	0.003394583320732613	0.0
34-35	0.0	6.64709140532096E-4	0.0	0.005891048184410409	0.0
36-37	0.0	7.154502962979049E-4	0.0	0.010051822957206736	0.0
38-39	0.0	7.357467586042285E-4	0.0	0.016627876744455566	0.0
40-41	0.0	9.031925726313977E-4	0.0	0.02390415848127256	0.0
42-43	0.0	0.0010351195776225008	0.0	0.030652732198125136	0.0
44-45	0.0	0.001156898351460442	0.0	0.038507463110672345	0.0
46-47	0.0	0.001156898351460442	0.0	0.047209571324508565	0.0
48-49	0.0	0.0011670465826136038	0.0	0.056074051236795375	0.0
50-51	0.0	0.0012786771252983834	0.0	0.06449200897834306	0.0
52-53	0.0	0.0012786771252983834	0.0	0.0731383019208369	0.0
54-55	0.0	0.0012888253564515453	0.0	0.08185055836582628	0.0
56-57	0.0	0.001298973587604707	0.0	0.09033447960986953	0.0
58-59	0.0	0.001304047703181288	0.0	0.09828561871837177	0.0
60-61	0.0	0.0013192700499110306	0.0	0.10604394143496396	0.0
62-63	1.0148231153161773E-5	0.0013497147433705159	0.0	0.11329485259389803	0.0
64-65	1.0148231153161773E-5	0.001517160557397685	0.0	0.12091617418992252	0.0
66-67	1.0148231153161773E-5	0.0015425311352805894	0.0	0.12924787196666832	0.0
68-69	1.0148231153161773E-5	0.001567901713163494	0.0	0.1373106416178554	0.0
70-71	1.0148231153161773E-5	0.0015831240598932366	0.0	0.14509433491233042	0.0
72-73	1.0148231153161773E-5	0.00160342052219956	0.0	0.1511325324484617	0.0
74-75	1.0148231153161773E-5	0.0016693840246951117	0.0	0.1565516878842501	0.0
76-77	1.0148231153161773E-5	0.0017759404518033102	0.0	0.1612959859483532	0.0
78-79	1.0148231153161773E-5	0.001826681607569119	0.0	0.1656242065351767	0.0
80-81	1.0148231153161773E-5	0.0018469780698754427	0.0	0.16808007847424186	0.0
82-83	1.0148231153161773E-5	0.0018571263010286043	0.0	0.17005898354910842	0.0
84-85	1.0148231153161773E-5	0.001877422763334928	0.0	0.17142392063920867	0.0
86-87	1.0148231153161773E-5	0.0018977192256412515	0.0	0.17164210760900164	0.0
88-89	1.0148231153161773E-5	0.0019484603814070604	0.0	0.17169792288034402	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12465	0.0	43.88351	1
GTATCAA	21195	0.0	32.89921	1
ATCAACG	24615	0.0	27.150864	3
CAACGCA	25930	0.0	25.70906	5
ACGCAGA	26055	0.0	25.617945	7
AACGCAG	26345	0.0	25.335949	6
TCAACGC	26440	0.0	25.292545	4
TATCAAC	27570	0.0	24.804552	2
CGCAGAG	30585	0.0	21.823624	8
GTACATG	27800	0.0	20.386292	1
ACATGGG	26640	0.0	20.028654	3
TACATGG	27775	0.0	19.8453	2
GCAGAGT	35790	0.0	18.426918	9
AGAGTAC	32960	0.0	18.181393	10-11
GAGTACT	20580	0.0	18.011744	12-13
CATGGGG	20655	0.0	17.173933	4
CAGAGTA	35770	0.0	16.77658	10-11
AGTACTT	23600	0.0	15.769111	12-13
GTACTTT	24080	0.0	15.333596	14-15
ATGGGGG	13705	0.0	15.315431	5
>>END_MODULE