FastQCFastQC Report
Fri 27 May 2016
SRR2138604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24105868
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA287850.048.0378341
GTATCAA519250.034.472021
ATCAACG637500.027.413113
TCAACGC642700.027.2043674
CAACGCA644000.027.124195
AACGCAG668200.026.217276
TATCAAC672100.026.2076232
ACGCAGA772650.022.6518677
CGCAGAG776750.022.5107048
GTACATG645450.020.5583341
TACATGG641650.020.235212
ACATGGG652050.019.4884263
GCAGAGT908800.019.069159
AGAGTAC819050.018.78867710-11
GAGTACT560200.018.34438912-13
CATGGGG499550.017.6049374
CAGAGTA889900.017.3587810-11
AGTACTT629550.016.3469212-13
GTGGTAT138800.016.180441
GTACTTT659850.015.55577414-15
Produced by FastQC (version 0.11.5)