FastQCFastQC Report
Thu 26 May 2016
SRR2132792_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132792_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289565
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1544866.747395247568861No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA674472.9458434244059464No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT124620.5442955321207305No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG116730.5098348376219937No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53890.23537222136082617No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53720.2346297222398141No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48290.21091342678631092No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43940.19191418457217857No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC43790.19125903828893262No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36830.16086025074632082No Hit
AACGAGTGGAATCACTGCTGAGTAACGAGTGGAATCACTGCTGAGTAACG35910.156842020209079No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA31700.13845424785930951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT31700.083.1624451
ATCGAGT10450.071.627411
AACGTGT15350.070.843841
ACGTGTG16350.068.722592
GTGGAAT573250.064.816026
TGGAATC577250.064.296127
GAATCAC578250.064.274339
GGAATCA579100.064.188118
ACGAGTG537600.063.1826672
CGAGTGG552950.063.1732673
AACGAGT495500.063.151041
AGTGGAA575400.062.3614585
GAGTGGA576000.062.3536224
CGTGTGG19950.059.1429183
TCGAGTG13550.057.2484862
TGAGTGG21700.051.1239283
AATGAGT19450.049.859291
ATGAGTG21350.048.444462
GTGTGGA29650.043.595534
AGTACTT251050.041.23274220-21
Produced by FastQC (version 0.11.5)