##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132792_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2289565 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.88076293968505 31.0 25.0 33.0 16.0 34.0 2 28.143291848014798 31.0 25.0 34.0 16.0 34.0 3 29.199771135565054 31.0 28.0 34.0 16.0 34.0 4 34.7247839655131 35.0 35.0 37.0 32.0 37.0 5 33.80580153872024 35.0 35.0 37.0 28.0 37.0 6 35.23203097531627 37.0 35.0 37.0 32.0 37.0 7 35.62830275620041 37.0 35.0 37.0 33.0 37.0 8 35.899376082356255 37.0 35.0 37.0 35.0 37.0 9 37.86285517117881 39.0 38.0 39.0 35.0 39.0 10-11 37.85622137829675 39.0 38.0 39.0 35.0 39.0 12-13 37.83232295217651 39.0 38.0 39.0 35.0 39.0 14-15 39.242932827851575 41.0 39.0 41.0 36.0 41.0 16-17 39.20501994920433 40.5 39.0 41.0 36.0 41.0 18-19 39.03547464256311 40.0 39.0 41.0 35.5 41.0 20-21 38.94920694542413 40.0 39.0 41.0 35.0 41.0 22-23 38.806331333681285 40.0 39.0 41.0 35.0 41.0 24-25 38.66113584894947 40.0 38.5 41.0 35.0 41.0 26-27 38.458253642067376 40.0 38.0 41.0 34.5 41.0 28-29 38.28117852081073 40.0 38.0 41.0 34.0 41.0 30-31 38.15000818932854 40.0 38.0 41.0 34.0 41.0 32-33 37.9992411222219 40.0 38.0 41.0 34.0 41.0 34-35 37.89965932393271 40.0 38.0 41.0 33.5 41.0 36-37 37.736774452789064 40.0 37.5 41.0 33.0 41.0 38-39 37.27216458148163 40.0 36.0 41.0 33.0 41.0 40-41 37.019547818035306 40.0 35.0 41.0 33.0 41.0 42-43 36.8547180359588 40.0 35.0 41.0 33.0 41.0 44-45 36.706042632552474 39.0 35.0 41.0 32.0 41.0 46-47 36.56128980832604 39.0 35.0 41.0 32.0 41.0 48-49 36.39024487184247 39.0 35.0 41.0 31.0 41.0 50-51 36.097888900293285 39.0 35.0 41.0 31.0 41.0 52-53 35.64451697156447 39.0 35.0 41.0 29.5 41.0 54-55 34.79673737150944 38.5 35.0 40.5 25.0 41.0 56-57 34.093427354104385 38.0 35.0 40.0 19.0 41.0 58-59 33.72667471768655 37.5 34.0 40.0 14.0 41.0 60-61 33.45175677475852 37.0 34.0 40.0 9.5 41.0 62-63 33.158317191256856 36.5 34.0 40.0 7.5 41.0 64-65 32.86205545594906 36.0 34.0 39.5 6.5 41.0 66-67 32.53763597015154 35.5 33.5 39.0 2.0 41.0 68-69 32.22577607536803 35.0 33.0 39.0 2.0 40.5 70-71 31.914940610989426 35.0 33.0 38.0 2.0 40.0 72-73 31.614526776920506 35.0 33.0 37.0 2.0 40.0 74-75 31.306300323423883 35.0 33.0 37.0 2.0 39.0 76-77 31.00793753398571 35.0 33.0 36.0 2.0 39.0 78-79 30.728261481984568 35.0 33.0 36.0 2.0 38.5 80-81 30.463723458386198 35.0 33.0 35.0 2.0 37.0 82-83 30.214699953921375 35.0 32.0 35.0 2.0 37.0 84-85 29.98207017490222 35.0 32.0 35.0 2.0 36.5 86-87 29.812692804091608 35.0 32.0 35.0 2.0 36.0 88-89 29.633065888061708 35.0 32.0 35.0 2.0 36.0 90-91 29.46600598803703 35.0 32.0 35.0 2.0 35.5 92-93 29.31528368925975 35.0 32.0 35.0 2.0 35.0 94-95 29.151136569610387 35.0 31.5 35.0 2.0 35.0 96-97 28.975325007152012 35.0 31.0 35.0 2.0 35.0 98-99 28.81309702934837 35.0 31.0 35.0 2.0 35.0 100 28.717468602114376 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2345729739202369 1101 2 2.261021199352829 1101 3 1.2003239633588407 1101 4 0.7107389576054004 1101 5 2.2810022728524544 1101 6 1.0277732385587512 1101 7 0.5796397250664143 1101 8 0.4359046299833125 1101 9 0.36257438635121986 1101 10-11 0.5010554608624105 1101 12-13 0.44061154564759875 1101 14-15 0.5423561194672004 1101 16-17 0.4346516660890174 1101 18-19 0.35139041986911934 1101 20-21 0.3726427477284773 1101 22-23 0.38447608943737066 1101 24-25 0.2826826315077611 1101 26-27 0.20013648501250714 1101 28-29 0.33947535256854167 1101 30-31 0.37982805976542977 1101 32-33 0.3934587594179888 1101 34-35 0.429952176176684 1101 36-37 0.40561297808554997 1101 38-39 0.44093191697368894 1101 40-41 0.5621264059376685 1101 42-43 0.5853613747576034 1101 44-45 0.5903552822204787 1101 46-47 0.4887860361028231 1101 48-49 0.5257341321369395 1101 50-51 0.5110939910309398 1101 52-53 0.539239530330093 1101 54-55 0.6512261245360307 1101 56-57 0.698037296503955 1101 58-59 0.6998592575102904 1101 60-61 0.6202517601425797 1101 62-63 0.6167851629615271 1101 64-65 0.7272599762168426 1101 66-67 0.7197848328687044 1101 68-69 0.7057422843868295 1101 70-71 0.5827656345640655 1101 72-73 0.6207651900386679 1101 74-75 0.570063150064648 1101 76-77 0.47879829707740384 1101 78-79 0.5696129276150117 1101 80-81 0.6526592140965874 1101 82-83 0.674706416371663 1101 84-85 0.6256104421999709 1101 86-87 0.688708874021934 1101 88-89 0.6579194831336785 1101 90-91 0.6955723592692848 1101 92-93 0.716609689946317 1101 94-95 0.8026272219298463 1101 96-97 0.7759562587080957 1101 98-99 0.7281485004619341 1101 100 0.7937187749617358 1102 1 1.5504237676504538 1102 2 3.0698007012911788 1102 3 3.2846742082659404 1102 4 1.4112171253155097 1102 5 2.668071528664413 1102 6 1.2230874077998664 1102 7 0.7624543292537993 1102 8 0.5311473673870566 1102 9 0.44735785212389345 1102 10-11 0.5397275044714931 1102 12-13 0.476155642585546 1102 14-15 0.6490384470753554 1102 16-17 0.5770862897102447 1102 18-19 0.4760437209603481 1102 20-21 0.44082816050758566 1102 22-23 0.3344536016367172 1102 24-25 0.4368479358220654 1102 26-27 0.5045857912696121 1102 28-29 0.524481726508462 1102 30-31 0.5370440269090864 1102 32-33 0.5643382222183675 1102 34-35 0.5685422342107387 1102 36-37 0.5767976221959756 1102 38-39 0.5528072873969379 1102 40-41 0.5882167838908785 1102 42-43 0.6910014129351012 1102 44-45 0.6175382739664173 1102 46-47 0.5950892055886499 1102 48-49 0.5521412660034812 1102 50-51 0.5593811510431408 1102 52-53 0.6473904666076606 1102 54-55 0.6802231657511086 1102 56-57 0.5913285126597358 1102 58-59 0.6955894510635332 1102 60-61 0.6726175659175695 1102 62-63 0.663667698136102 1102 64-65 0.670340822160739 1102 66-67 0.7319839614808146 1102 68-69 0.69831333016446 1102 70-71 0.7102265318158096 1102 72-73 0.7022890030954585 1102 74-75 0.7394343198415001 1102 76-77 0.7515819330902112 1102 78-79 0.7354164595800405 1102 80-81 0.7489240392941952 1102 82-83 0.8006377405839231 1102 84-85 0.776074461575142 1102 86-87 0.7895626340138371 1102 88-89 0.8634014740574685 1102 90-91 0.8161382437803404 1102 92-93 0.7884373643770246 1102 94-95 0.830941433443428 1102 96-97 0.8263247624440311 1102 98-99 0.9229581320272153 1102 100 0.9981787499835981 1103 1 1.2313107817270819 1103 2 2.936791316871801 1103 3 3.226558450076773 1103 4 1.4251170196631051 1103 5 2.632920047311174 1103 6 1.3078733229583221 1103 7 0.7972413819512667 1103 8 0.547518634594411 1103 9 0.49694068050088447 1103 10-11 0.5111710442487336 1103 12-13 0.557570141824236 1103 14-15 0.6405903471539247 1103 16-17 0.5741617740244109 1103 18-19 0.5380688677861158 1103 20-21 0.49162884924584205 1103 22-23 0.4678283164196202 1103 24-25 0.5210688270311579 1103 26-27 0.5908750613358009 1103 28-29 0.5848168649219829 1103 30-31 0.5976505793557436 1103 32-33 0.6879146337655513 1103 34-35 0.6633730349938105 1103 36-37 0.6652170034191442 1103 38-39 0.708407017384026 1103 40-41 0.7412119657195504 1103 42-43 0.6807942199152635 1103 44-45 0.7728929475315667 1103 46-47 0.7783787852874156 1103 48-49 0.7367981814638043 1103 50-51 0.8630415712334027 1103 52-53 0.7714141945616007 1103 54-55 0.706534720483937 1103 56-57 0.7042133754003927 1103 58-59 0.7544478696290611 1103 60-61 0.8457601589294228 1103 62-63 0.8668921634372779 1103 64-65 0.8547911771838912 1103 66-67 0.852484578526358 1103 68-69 0.8187189904475858 1103 70-71 0.7884580026925967 1103 72-73 0.8050013538836325 1103 74-75 0.8772150561455305 1103 76-77 0.8541398837665213 1103 78-79 0.884579799638729 1103 80-81 0.882594444857375 1103 82-83 0.9220498454417125 1103 84-85 0.9290060383262855 1103 86-87 0.9411509436641623 1103 88-89 0.9977209011151373 1103 90-91 1.0007779574312536 1103 92-93 1.0168842601717927 1103 94-95 1.0219598277266613 1103 96-97 1.003801656529717 1103 98-99 1.0589455103382868 1103 100 1.123788581871981 1104 1 6.074617069759533 1104 2 5.1218825947622655 1104 3 4.139826343441015 1104 4 1.8717448087097566 1104 5 2.8873300009477987 1104 6 1.290677220379358 1104 7 0.732006667375785 1104 8 0.571162143722205 1104 9 0.4535836825292847 1104 10-11 0.49849808713485544 1104 12-13 0.38997379293984835 1104 14-15 0.5019575233347382 1104 16-17 0.3771672434535631 1104 18-19 0.4431778941552835 1104 20-21 0.3886423960438634 1104 22-23 0.3773699347911048 1104 24-25 0.38998363433339023 1104 26-27 0.4585221201593299 1104 28-29 0.49485652733867624 1104 30-31 0.5894917123606689 1104 32-33 0.565576905570822 1104 34-35 0.602901432559733 1104 36-37 0.5736946186031489 1104 38-39 0.5563541703831518 1104 40-41 0.5611871540707511 1104 42-43 0.6204765583678906 1104 44-45 0.6345182010812636 1104 46-47 0.513500851355218 1104 48-49 0.7028813819853852 1104 50-51 0.7327577766811046 1104 52-53 0.6199680557612552 1104 54-55 0.6646265779165645 1104 56-57 0.623480435672775 1104 58-59 0.6451905891306566 1104 60-61 0.6560868758230711 1104 62-63 0.7265116577867445 1104 64-65 0.6895084692731359 1104 66-67 0.6773541815399824 1104 68-69 0.7036119562545977 1104 70-71 0.6921906164277409 1104 72-73 0.7257806569841847 1104 74-75 0.7488238289650084 1104 76-77 0.7214835884897894 1104 78-79 0.6916239535071682 1104 80-81 0.698798081484437 1104 82-83 0.6734398410951457 1104 84-85 0.6836604863123767 1104 86-87 0.697480630711393 1104 88-89 0.6403833540273354 1104 90-91 0.6873588397841957 1104 92-93 0.7971879764859011 1104 94-95 0.7748711215545327 1104 96-97 0.7348216507342968 1104 98-99 0.7813357595414168 1104 100 0.8507155595596068 1105 1 1.6623140049341742 1105 2 3.119455555997277 1105 3 3.2852463669597327 1105 4 1.3923404624131663 1105 5 2.570147261148378 1105 6 1.171990994451832 1105 7 0.6920454265983338 1105 8 0.4260387910415915 1105 9 0.3671438793818851 1105 10-11 0.4188403942968435 1105 12-13 0.3728894977909647 1105 14-15 0.5017295317628907 1105 16-17 0.4007448093450279 1105 18-19 0.4011467074771744 1105 20-21 0.31085293652872537 1105 22-23 0.2860319499666062 1105 24-25 0.3436090411007626 1105 26-27 0.3367415262986242 1105 28-29 0.34237882450809565 1105 30-31 0.3189003838782227 1105 32-33 0.34671744799023685 1105 34-35 0.33093799325935436 1105 36-37 0.35522502071238904 1105 38-39 0.38650254643307136 1105 40-41 0.4068658258032585 1105 42-43 0.429592236795834 1105 44-45 0.4461294009394976 1105 46-47 0.4515265950704048 1105 48-49 0.3777586833306046 1105 50-51 0.328849736383674 1105 52-53 0.4743593280944651 1105 54-55 0.47076847032918323 1105 56-57 0.37561434241682434 1105 58-59 0.48653293194294633 1105 60-61 0.41813720356996953 1105 62-63 0.4021359116861234 1105 64-65 0.40481669107256835 1105 66-67 0.42848067223393826 1105 68-69 0.36594408525418487 1105 70-71 0.4064559029382373 1105 72-73 0.40546196815001423 1105 74-75 0.4683991761491839 1105 76-77 0.42976840507682823 1105 78-79 0.4122904687474147 1105 80-81 0.41759462676006365 1105 82-83 0.3710399529217696 1105 84-85 0.35212377986590937 1105 86-87 0.4576951970594969 1105 88-89 0.38884891635586527 1105 90-91 0.3914509444472003 1105 92-93 0.44100602990578963 1105 94-95 0.427090752867457 1105 96-97 0.3974913435702092 1105 98-99 0.40474476632912726 1105 100 0.4398567253351011 1106 1 -0.6108292297418458 1106 2 1.9131706064018772 1106 3 2.7520684616750515 1106 4 1.1812419555276534 1106 5 2.4415688210844095 1106 6 1.1475574585417476 1106 7 0.6389707109327318 1106 8 0.4382591727285998 1106 9 0.3048474501866991 1106 10-11 0.44790633674951863 1106 12-13 0.3557320951637948 1106 14-15 0.4623916722247401 1106 16-17 0.402972592220344 1106 18-19 0.3704706650879004 1106 20-21 0.3455262637786092 1106 22-23 0.3600352793741166 1106 24-25 0.37538137041691044 1106 26-27 0.35785586597165775 1106 28-29 0.4155062051783531 1106 30-31 0.3984117701840475 1106 32-33 0.39843548910614857 1106 34-35 0.42415533662662597 1106 36-37 0.4655693980968678 1106 38-39 0.5177970541837595 1106 40-41 0.5216072530032676 1106 42-43 0.4956759897088645 1106 44-45 0.4794855063098282 1106 46-47 0.5273770056632969 1106 48-49 0.6007610222440363 1106 50-51 0.7059709473193934 1106 52-53 0.7021227754541997 1106 54-55 0.5591444406162154 1106 56-57 0.4790791355833761 1106 58-59 0.5611134970139275 1106 60-61 0.5230259728999513 1106 62-63 0.4559320476054225 1106 64-65 0.5372928560237256 1106 66-67 0.45205055935687 1106 68-69 0.4319475072262833 1106 70-71 0.4908297479530326 1106 72-73 0.45370472633061354 1106 74-75 0.526188983197116 1106 76-77 0.49060375933726874 1106 78-79 0.5919229183895638 1106 80-81 0.5096159243060043 1106 82-83 0.5001430471913686 1106 84-85 0.4885692812735414 1106 86-87 0.48698098039922755 1106 88-89 0.47446890213699433 1106 90-91 0.44991429808860417 1106 92-93 0.4633719143663555 1106 94-95 0.4556275322379122 1106 96-97 0.5464514640155649 1106 98-99 0.5666807042106008 1106 100 0.5895553400888645 1107 1 -1.097566582423088 1107 2 1.6713473776213803 1107 3 -0.8432927996018442 1107 4 -0.10732393578509658 1107 5 1.8067458376917358 1107 6 0.7376303505680752 1107 7 0.26635632261352526 1107 8 0.25729157356066423 1107 9 0.16981570812868796 1107 10-11 0.25983412663144634 1107 12-13 0.28017915322266873 1107 14-15 0.3582223375507283 1107 16-17 0.22466343000080968 1107 18-19 0.22684270662922046 1107 20-21 0.21027454021143654 1107 22-23 0.1676837920237091 1107 24-25 0.04685148308875142 1107 26-27 -0.013925071277633094 1107 28-29 0.004628942329439667 1107 30-31 -0.009488753833252872 1107 32-33 -0.021407380064133008 1107 34-35 -0.002322711903985919 1107 36-37 0.03417257679181063 1107 38-39 0.09270595636380108 1107 40-41 0.0391471120002862 1107 42-43 0.0349376284864249 1107 44-45 0.11672780434064123 1107 46-47 0.14347154139146312 1107 48-49 0.10660605831824199 1107 50-51 0.20434122515294462 1107 52-53 0.1997139262900447 1107 54-55 0.06565155562379488 1107 56-57 -0.0026661860054844055 1107 58-59 0.005183318800675352 1107 60-61 -0.008732402990673904 1107 62-63 0.04850976820865327 1107 64-65 -0.022312975668874913 1107 66-67 -0.06045712794688285 1107 68-69 -0.056508868409316904 1107 70-71 0.03968870834039251 1107 72-73 0.019449522442521072 1107 74-75 -0.04431064954736996 1107 76-77 -0.010525134817406467 1107 78-79 0.023455790856466763 1107 80-81 0.024749757062771494 1107 82-83 0.021502941897686867 1107 84-85 0.10239556321836574 1107 86-87 -0.010989956979511106 1107 88-89 -0.0550011840957616 1107 90-91 -0.08994531859413257 1107 92-93 -0.04193821205381454 1107 94-95 -0.07152383184022781 1107 96-97 -0.016352258498450567 1107 98-99 0.010470987678981913 1107 100 0.017437345725348052 1108 1 -1.167545251678483 1108 2 0.46493969641721833 1108 3 2.09247227325595 1108 4 0.8915226394533704 1108 5 0.7527400410521494 1108 6 0.4612631240252938 1108 7 0.2717135451106998 1108 8 0.17980781337428908 1108 9 0.14723606351023477 1108 10-11 0.241819721775677 1108 12-13 0.2613646469884898 1108 14-15 0.351877851870789 1108 16-17 0.23066678830493004 1108 18-19 0.17688158230456708 1108 20-21 0.15836483309891491 1108 22-23 0.18601204561175422 1108 24-25 0.08512482722096593 1108 26-27 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-0.7791552100617807 2215 62-63 -0.8432298296727581 2215 64-65 -0.8236091225094455 2215 66-67 -0.8220768337162632 2215 68-69 -0.7453659741069529 2215 70-71 -0.7208003807555734 2215 72-73 -0.6401110809007093 2215 74-75 -0.74724044287872 2215 76-77 -0.7409411877616208 2215 78-79 -0.8083919684523977 2215 80-81 -0.7363992426650299 2215 82-83 -0.7348977380940731 2215 84-85 -0.8062582095529649 2215 86-87 -0.8464078002125355 2215 88-89 -0.8158886363017253 2215 90-91 -0.8631795627806227 2215 92-93 -0.8556757531700399 2215 94-95 -0.8608293365172379 2215 96-97 -0.8720558547212285 2215 98-99 -0.879866270298141 2215 100 -0.9505249112978014 2216 1 2.9746400534010213 2216 2 -1.741552620554323 2216 3 -2.248000717425505 2216 4 -0.8599084082704067 2216 5 -2.425791104601835 2216 6 -1.0603650695209694 2216 7 -0.48881505253024216 2216 8 -0.3893242339099743 2216 9 -0.4203966463921631 2216 10-11 -0.20667013123163258 2216 12-13 -0.24364689671532602 2216 14-15 -0.2899146584330339 2216 16-17 -0.41739913184376576 2216 18-19 -0.3498963685440941 2216 20-21 -0.3131184062676269 2216 22-23 -0.30063836850541037 2216 24-25 -0.3026457307343904 2216 26-27 -0.36179140589609915 2216 28-29 -0.409910024531392 2216 30-31 -0.4490186558153795 2216 32-33 -0.39367638145472483 2216 34-35 -0.5419949221617628 2216 36-37 -0.44854296658225223 2216 38-39 -0.49795471516561207 2216 40-41 -0.42123400839076197 2216 42-43 -0.379107547167159 2216 44-45 -0.32884660403205146 2216 46-47 -0.3754589746987449 2216 48-49 -0.32663891658305744 2216 50-51 -0.3720759020148918 2216 52-53 -0.38727635155265006 2216 54-55 -0.1597090853986245 2216 56-57 0.1554459383213711 2216 58-59 0.21200823035236027 2216 60-61 0.2075301025804066 2216 62-63 0.19819891244549126 2216 64-65 0.1855164296391294 2216 66-67 0.12165442086170941 2216 68-69 0.1438170943753434 2216 70-71 0.13260461383022815 2216 72-73 0.18355368805222838 2216 74-75 0.09312972299217748 2216 76-77 0.2017687315720771 2216 78-79 0.24342743972428238 2216 80-81 0.2338098286789716 2216 82-83 0.18123512616526227 2216 84-85 0.2838217366224711 2216 86-87 0.32328232201555096 2216 88-89 0.2581845491766934 2216 90-91 0.2244281701211399 2216 92-93 0.14699170348401935 2216 94-95 0.04464343757595657 2216 96-97 0.019637307336800802 2216 98-99 -0.1132523225284281 2216 100 -0.02900885563440525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1199.0 3 269.0 4 326.0 5 871.0 6 2645.0 7 4580.0 8 5378.0 9 7463.0 10 5637.0 11 5199.0 12 4512.0 13 6279.0 14 6383.0 15 6659.0 16 7978.0 17 9120.0 18 13073.0 19 23584.0 20 47241.0 21 54456.0 22 23586.0 23 13821.0 24 13643.0 25 14982.0 26 16875.0 27 19249.0 28 22937.0 29 27325.0 30 32278.0 31 39953.0 32 52428.0 33 74434.0 34 111356.0 35 223632.0 36 322594.0 37 516494.0 38 452554.0 39 98038.0 40 534.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.617444275135014 40.72415645124326 24.30117581608214 20.357223457539586 2 15.81218215355693 39.788270535342626 24.090745691833472 20.30880161926697 3 19.67798970251916 24.72880446841483 18.01010879269288 37.58309703637314 4 32.990720944808295 26.551288126783906 20.318270064400878 20.139720864006918 5 18.106117485812522 38.257424376037065 20.939288994762656 22.69716914338776 6 37.263064461667696 22.887471754256683 22.49507815329079 17.354385630784837 7 20.229177176626287 22.437568109998058 37.93661921078666 19.396635502589 8 36.3281234645009 22.83307091084988 21.981730153981218 18.857075470668008 9 35.64742647620836 24.747102615562344 22.00278218788285 17.60268872034644 10-11 17.704869702323368 41.93918932198911 22.057159329392263 18.298781646295257 12-13 17.613433119391676 24.44842142503052 30.473081131131895 27.46506432444591 14-15 17.896325284497276 32.724775230229326 21.964499806731848 27.414399678541557 16-17 26.95629955908655 23.826687602230116 30.58952246387414 18.627490374809188 18-19 18.246719354986645 24.07411451520267 30.82851982800226 26.850646301808425 20-21 27.056777160727037 32.99993666919262 21.988980439515803 17.954305730564542 22-23 26.47500726120464 24.386357233797685 30.974682963794432 18.16395254120324 24-25 17.955353964617732 33.06512372437559 21.93632851655227 27.043193794454407 26-27 18.63720837801067 27.716378438699053 35.14248339750127 18.503929785789 28-29 26.286346969839247 24.13585113329388 31.352898913112316 18.224902983754557 30-31 24.202261128205578 25.216536765717507 31.739566249484074 18.841635856592845 32-33 20.581857252360162 26.861478053691425 32.481563091678986 20.075101602269427 34-35 20.148936588391244 26.560132601607727 32.93477145221909 20.356159357781937 36-37 20.316959771834387 26.581665076117076 32.95722113152498 20.14415402052355 38-39 20.97695413757635 25.97532719097296 32.70924826331639 20.338470408134295 40-41 20.05887581265437 27.21407778333439 32.45494231436976 20.27210408964148 42-43 20.030377214686762 26.426342351864673 33.21922041278944 20.324060020659125 44-45 20.18278581302562 26.900153522612374 32.35627728411292 20.560783380249088 46-47 20.64695695470537 26.321659354506206 32.8185703397807 20.212813351007725 48-49 20.10412458261722 26.6569195458526 32.611544114274984 20.6274117572552 50-51 20.664252816583062 27.10803143828631 31.8654635269145 20.362252218216124 52-53 20.864361829904585 26.75712647222482 31.80929365529302 20.569218042577575 54-55 21.2682321751075 27.618848995333174 29.88441035742597 21.228508472133353 56-57 21.47119649365709 28.478269889695202 28.537123864140128 21.513409752507574 58-59 22.127587554841202 28.61364495002326 27.62092362523012 21.637843869905417 60-61 21.81877343512851 29.119265013223032 27.257230085190855 21.804731466457604 62-63 21.239507006466514 29.114676225402793 27.04345173217515 22.602365035955543 64-65 21.51264541517712 28.90885386525388 27.914407321914865 21.66409339765414 66-67 21.76874209729796 28.535071072452627 28.073957280094692 21.622229550154724 68-69 21.97081104926045 28.5811715325837 27.99365818397818 21.454359234177673 70-71 20.56012823396584 29.632397420470703 28.164389305392074 21.643085040171385 72-73 20.801265574546647 28.893412819008773 28.31851894234377 21.98680266410081 74-75 21.111224912968876 29.18067685219985 27.69272362811193 22.015374606719345 76-77 21.904418314607305 28.501938918551446 27.885027163640707 21.708615603200542 78-79 21.581291540595814 29.094921387476376 27.264869445011637 22.058917626916177 80-81 21.716664359069664 29.519872777882362 27.16166879009268 21.6017940729553 82-83 21.489096805880497 29.708536949962124 26.9645960938365 21.837770150320885 84-85 21.549690897798417 29.81342491528844 26.534722786383604 22.10216140052954 86-87 21.65238519315695 29.984717607683216 26.51062670037907 21.852270498780772 88-89 22.39862157222005 29.381607423244155 26.561202673870365 21.65856833066543 90-91 21.808450699304057 29.95848114180791 26.480435996055142 21.752632162832892 92-93 21.211125257417894 29.992400303114348 26.658339029466294 22.138135410001464 94-95 21.31870027712688 30.266011229207297 26.33166125443043 22.0836272392354 96-97 21.469428098344775 29.57423269420848 26.66589358459811 22.290445622848637 98-99 22.141944455909577 29.531888464569793 25.9805363541883 22.34563072533233 100 21.505220423966996 30.316195434503936 25.81691281968409 22.361671321844977 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 71657.0 1 38638.5 2 4006.5 3 2041.0 4 1633.0 5 1470.5 6 1463.5 7 1606.5 8 1552.0 9 1479.0 10 1460.0 11 2329.0 12 17596.0 13 19427.0 14 5799.0 15 4874.5 16 5337.0 17 5900.5 18 6537.5 19 7241.5 20 8061.0 21 9107.0 22 10410.0 23 12003.5 24 13726.0 25 15604.5 26 17915.5 27 20160.5 28 22208.0 29 24536.5 30 26731.0 31 28742.5 32 31232.5 33 34682.0 34 38720.0 35 41938.0 36 44349.0 37 46606.5 38 48098.0 39 49117.0 40 50301.0 41 51559.5 42 52773.0 43 54755.0 44 58556.5 45 62636.5 46 66776.5 47 73510.0 48 80738.0 49 85804.5 50 96524.5 51 96904.0 52 83800.0 53 79251.5 54 79883.0 55 81035.5 56 76077.0 57 66391.5 58 57435.5 59 48056.0 60 40185.5 61 33545.5 62 27064.5 63 20164.0 64 14330.5 65 10458.0 66 7848.5 67 5916.5 68 4288.5 69 3157.0 70 2524.0 71 2066.5 72 1650.5 73 1128.0 74 734.5 75 553.5 76 411.0 77 277.0 78 165.5 79 79.5 80 31.0 81 16.0 82 12.5 83 5.5 84 3.5 85 3.0 86 2.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13771174873829745 2 0.005852640130330434 3 0.0031883785784635947 4 0.0 5 0.002009115268620895 6 0.012316750125023748 7 0.0030573493218144063 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 8.735283776612588E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.3102925664918883E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 6.551462832459442E-5 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 5.241170265967553E-4 74-75 4.586023982721608E-4 76-77 6.769844926874756E-4 78-79 5.459552360382868E-4 80-81 0.0011355868909596364 82-83 0.0012884543570503568 84-85 0.0013539689853749512 86-87 8.735283776612588E-5 88-89 0.0 90-91 1.3102925664918883E-4 92-93 0.0 94-95 0.0 96-97 0.0012229397287257622 98-99 6.333080738044126E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2289565.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.71053045147952 #Duplication Level Percentage of deduplicated Percentage of total 1 88.12358582922973 56.1440039846671 2 7.885992009701918 10.048414681484765 3 1.7430951771114707 3.3316055508356235 4 0.6765048425682884 1.7240192949208115 5 0.3602280279118263 1.1475159370876413 6 0.2172024039005326 0.8302848220703661 7 0.15398551184722248 0.6867349049140391 8 0.11611178425451434 0.5918034693218286 9 0.08445956775944752 0.4842867477291371 >10 0.58116777758119 7.0518051961186465 >50 0.034118471758082906 1.5028606176713324 >100 0.01943125413758963 2.5121428660553127 >500 0.00267627245466356 1.1257379460344057 >1k 0.0010293355594859451 1.5903032268049357 >5k 1.372447412647927E-4 0.4704674370762313 >10k+ 2.744894825295854E-4 10.758013317207887 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 154486 6.747395247568861 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 67447 2.9458434244059464 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 12462 0.5442955321207305 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 11673 0.5098348376219937 No Hit AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 5389 0.23537222136082617 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5372 0.2346297222398141 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 4829 0.21091342678631092 No Hit AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4394 0.19191418457217857 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC 4379 0.19125903828893262 No Hit ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3683 0.16086025074632082 No Hit AACGAGTGGAATCACTGCTGAGTAACGAGTGGAATCACTGCTGAGTAACG 3591 0.156842020209079 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA 3170 0.13845424785930951 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001965438849737832 0.0 0.0 0.0 0.0 2 0.001965438849737832 0.0 0.0 0.0 0.0 3 0.001965438849737832 0.0 0.0 0.0 0.0 4 0.001965438849737832 0.0 0.0 0.0 0.0 5 0.001965438849737832 0.0 0.0 0.0 0.0 6 0.001965438849737832 0.0 0.0 0.0 0.0 7 0.001965438849737832 0.0 0.0 0.0 0.0 8 0.001965438849737832 0.0 0.0 0.0 4.367641888306294E-5 9 0.002009115268620895 0.0 0.0 0.0 4.367641888306294E-5 10-11 0.0020309534780624267 0.0 0.0 0.0 4.367641888306294E-5 12-13 0.002096468106387021 0.0 0.0 0.0 4.367641888306294E-5 14-15 0.002096468106387021 0.0 0.0 0.0 4.367641888306294E-5 16-17 0.0021619827347116156 0.0 0.0 0.0 4.367641888306294E-5 18-19 0.002183820944153147 0.0 0.0 0.0 4.367641888306294E-5 20-21 0.002205659153594678 0.0 0.0 0.0 4.367641888306294E-5 22-23 0.002271173781919273 0.0 0.0 0.0 4.367641888306294E-5 24-25 0.002314850200802336 0.0 0.0 0.0 4.367641888306294E-5 26-27 0.0023366884102438675 0.0 0.0 0.0 4.367641888306294E-5 28-29 0.0023585266196853986 0.0 0.0 0.0 4.367641888306294E-5 30-31 0.0024022030385684617 0.0 0.0 0.0 4.367641888306294E-5 32-33 0.002467717666893056 0.0 0.0 0.0 4.367641888306294E-5 34-35 0.0025769087141007136 0.0 0.0 0.0 4.367641888306294E-5 36-37 0.0028171290179575597 0.0 0.0 0.0 4.367641888306294E-5 38-39 0.003494113510645035 0.0 0.0 0.0 4.367641888306294E-5 40-41 0.004171098003332511 0.0 0.0 0.0 4.367641888306294E-5 42-43 0.005525066988707462 0.0 0.0 0.0 4.367641888306294E-5 44-45 0.006769844926874756 0.0 0.0 0.0 4.367641888306294E-5 46-47 0.00816749033113277 0.0 0.0 0.0 4.367641888306294E-5 48-49 0.00993638529589682 0.0 0.0 0.0 4.367641888306294E-5 50-51 0.01218572086837456 0.0 0.0 0.0 4.367641888306294E-5 52-53 0.014740791373033742 0.0 0.0 0.0 4.367641888306294E-5 54-55 0.017667111438198962 0.0 0.0 0.0 4.367641888306294E-5 56-57 0.02157615092823309 0.0 0.0 0.0 4.367641888306294E-5 58-59 0.02603114565430551 0.0 0.0 0.0 4.367641888306294E-5 60-61 0.0320584914601682 0.0 0.0 0.0 4.367641888306294E-5 62-63 0.03924326236643205 0.0 0.0 0.0 4.367641888306294E-5 64-65 0.047716487629746264 0.0 0.0 0.0 4.367641888306294E-5 66-67 0.057871255020058396 0.0 0.0 0.0 4.367641888306294E-5 68-69 0.07243734071755989 0.0 0.0 0.0 4.367641888306294E-5 70-71 0.09259400803209344 0.0 0.0 0.0 4.367641888306294E-5 72-73 0.11497817270966318 0.0 0.0 0.0 4.367641888306294E-5 74-75 0.1414897589716824 0.0 0.0 0.0 4.367641888306294E-5 76-77 0.17370111789794132 0.0 0.0 0.0 4.367641888306294E-5 78-79 0.21106629425240164 0.0 0.0 0.0 4.367641888306294E-5 80-81 0.2585862379971741 0.0 0.0 0.0 4.367641888306294E-5 82-83 0.31796432946869824 0.0 0.0 0.0 4.367641888306294E-5 84-85 0.39321879920421565 0.0 0.0 0.0 4.367641888306294E-5 86-87 0.4800693581531863 0.0 0.0 0.0 4.367641888306294E-5 88 0.5560881652191574 0.0 0.0 0.0 4.367641888306294E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 3170 0.0 83.162445 1 ATCGAGT 1045 0.0 71.62741 1 AACGTGT 1535 0.0 70.84384 1 ACGTGTG 1635 0.0 68.72259 2 GTGGAAT 57325 0.0 64.81602 6 TGGAATC 57725 0.0 64.29612 7 GAATCAC 57825 0.0 64.27433 9 GGAATCA 57910 0.0 64.18811 8 ACGAGTG 53760 0.0 63.182667 2 CGAGTGG 55295 0.0 63.173267 3 AACGAGT 49550 0.0 63.15104 1 AGTGGAA 57540 0.0 62.361458 5 GAGTGGA 57600 0.0 62.353622 4 CGTGTGG 1995 0.0 59.142918 3 TCGAGTG 1355 0.0 57.248486 2 TGAGTGG 2170 0.0 51.123928 3 AATGAGT 1945 0.0 49.85929 1 ATGAGTG 2135 0.0 48.44446 2 GTGTGGA 2965 0.0 43.59553 4 AGTACTT 25105 0.0 41.232742 20-21 >>END_MODULE