FastQCFastQC Report
Thu 26 May 2016
SRR2132791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132791_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1945313
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1379037.088987736163794No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1194296.139320510375451No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG181190.9314182344949117No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109430.5625315823211997No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93330.47976855138479No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC61740.3173782316778842No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45600.23440957830436543No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43580.22402564523035626No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38560.19822002937316513No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA36610.1881959355640969No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32300.16604011796559218No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29580.1520577922421739No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29550.15190357541434207No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23920.12296221739123729No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23760.12213972764280093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT26950.085.760641
AACGTGT12600.073.223561
ACGTGTG14000.068.838672
ATCGAGT10000.066.842641
TCGAGTG10850.065.426532
CGTGTGG15650.064.282823
GTGGAAT526600.062.5422636
TGGAATC529200.062.2415547
GGAATCA536600.061.3975688
GAATCAC536900.061.3895269
AACGAGT459450.060.1402441
ACGAGTG499200.060.120832
CGAGTGG516500.060.1170963
GAGTGGA534100.059.7804834
AGTGGAA537000.059.4313855
AGCGAGT6750.057.1841131
ATGAGTG16550.053.4031072
TGAGTGG18400.053.142383
GCGAGTG7950.052.6295362
AATGAGT16800.047.632671
Produced by FastQC (version 0.11.5)