##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132791_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1945313
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.01729181884869	31.0	25.0	33.0	16.0	34.0
2	28.284251943003518	31.0	26.0	34.0	16.0	34.0
3	29.35441802938653	31.0	28.0	34.0	16.0	34.0
4	34.83423695826841	35.0	35.0	37.0	32.0	37.0
5	33.933179904724845	35.0	35.0	37.0	28.0	37.0
6	35.317979677306425	37.0	35.0	37.0	32.0	37.0
7	35.705389826727114	37.0	35.0	37.0	35.0	37.0
8	35.96449980028921	37.0	35.0	37.0	35.0	37.0
9	37.92941033139654	39.0	38.0	39.0	35.0	39.0
10-11	37.92040278351093	39.0	38.0	39.0	35.0	39.0
12-13	37.878719774144315	39.0	38.0	39.0	35.0	39.0
14-15	39.28347494721929	41.0	39.0	41.0	36.0	41.0
16-17	39.15989020789971	40.5	39.0	41.0	36.0	41.0
18-19	38.77676394492814	40.0	38.0	41.0	35.5	41.0
20-21	38.58181870989398	40.0	38.0	41.0	35.0	41.0
22-23	38.36980938286024	40.0	38.0	41.0	35.0	41.0
24-25	38.097993741881126	40.0	38.0	41.0	34.0	41.0
26-27	37.85414686479759	40.0	38.0	41.0	34.0	41.0
28-29	37.709694532448	40.0	37.5	41.0	33.5	41.0
30-31	37.58327503080481	40.0	37.0	41.0	33.0	41.0
32-33	37.43465267543064	40.0	37.0	41.0	33.0	41.0
34-35	37.331856364502784	40.0	37.0	41.0	33.0	41.0
36-37	37.1372861333883	40.0	36.0	41.0	33.0	41.0
38-39	36.65202643482051	39.0	35.0	41.0	32.5	41.0
40-41	36.3988640902518	39.0	35.0	41.0	32.0	41.0
42-43	36.22733359618735	39.0	35.0	41.0	31.0	41.0
44-45	36.08897437070539	39.0	35.0	41.0	31.0	41.0
46-47	35.94865736259409	39.0	35.0	41.0	31.0	41.0
48-49	35.77803726186994	39.0	35.0	41.0	31.0	41.0
50-51	35.477972182368596	38.0	35.0	41.0	29.5	41.0
52-53	34.989256227661045	38.0	35.0	40.0	27.5	41.0
54-55	34.119974266351996	38.0	34.5	40.0	21.5	41.0
56-57	33.442043773932525	37.0	34.0	40.0	8.5	41.0
58-59	33.10491782042273	37.0	34.0	40.0	2.0	41.0
60-61	32.84384646583866	36.5	34.0	40.0	2.0	41.0
62-63	32.57765228526206	36.0	34.0	40.0	2.0	41.0
64-65	32.29459295239378	35.0	33.0	39.5	2.0	41.0
66-67	31.975189082682324	35.0	33.0	39.0	2.0	41.0
68-69	31.686476931989866	35.0	33.0	38.5	2.0	40.5
70-71	31.396454709344972	35.0	33.0	37.5	2.0	40.0
72-73	31.11488254075308	35.0	33.0	37.0	2.0	40.0
74-75	30.82301254348272	35.0	33.0	36.5	2.0	39.0
76-77	30.536774801792824	35.0	33.0	36.0	2.0	39.0
78-79	30.246439776015478	35.0	32.5	36.0	2.0	38.5
80-81	29.972943428641045	35.0	32.0	35.0	2.0	37.0
82-83	29.70580621216226	35.0	32.0	35.0	2.0	37.0
84-85	29.45471191525477	35.0	32.0	35.0	2.0	36.5
86-87	29.28468117984098	35.0	32.0	35.0	2.0	36.0
88-89	29.086707640364303	35.0	31.0	35.0	2.0	36.0
90-91	28.90577557441913	35.0	31.0	35.0	2.0	35.5
92-93	28.756309138940622	35.0	31.0	35.0	2.0	35.0
94-95	28.577275225118015	35.0	31.0	35.0	2.0	35.0
96-97	28.38034213517311	35.0	31.0	35.0	2.0	35.0
98-99	28.22344347670529	34.5	31.0	35.0	2.0	35.0
100	28.134338278724297	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.20062115766032562
1101	2	2.240107212397273
1101	3	1.2710832550772118
1101	4	0.6712522513005865
1101	5	2.185455657513913
1101	6	0.962354950214241
1101	7	0.5491200110220404
1101	8	0.3863125545540811
1101	9	0.3581354489700459
1101	10-11	0.4866552489111484
1101	12-13	0.3977408916056504
1101	14-15	0.46594178127783437
1101	16-17	0.39000144342000453
1101	18-19	0.3824648416127516
1101	20-21	0.3619762144312375
1101	22-23	0.3473406648993276
1101	24-25	0.3087976222405331
1101	26-27	0.21257429041602194
1101	28-29	0.3184665598256231
1101	30-31	0.3184761793025359
1101	32-33	0.3549098377599904
1101	34-35	0.31861556336260577
1101	36-37	0.2734911237198858
1101	38-39	0.2973707712456104
1101	40-41	0.31563057008465023
1101	42-43	0.321834229554959
1101	44-45	0.3841952679481011
1101	46-47	0.32110171507362395
1101	48-49	0.29509931326261096
1101	50-51	0.37645155438642774
1101	52-53	0.5354903304833059
1101	54-55	0.5827363330622148
1101	56-57	0.37655548108136827
1101	58-59	0.3004474533984194
1101	60-61	0.25575868591199225
1101	62-63	0.2469992168328332
1101	64-65	0.23450255375090734
1101	66-67	0.17346093925468153
1101	68-69	0.2801076122134667
1101	70-71	0.24992609070683613
1101	72-73	0.34151035774847216
1101	74-75	0.38315408221011893
1101	76-77	0.28081270088170385
1101	78-79	0.2538017892568938
1101	80-81	0.25753827924829764
1101	82-83	0.24334183597888526
1101	84-85	0.32646563748969015
1101	86-87	0.31900316070066737
1101	88-89	0.3260483995304675
1101	90-91	0.36778652396292344
1101	92-93	0.3965990644134223
1101	94-95	0.4908891025237523
1101	96-97	0.43631440823562784
1101	98-99	0.3938072955567016
1101	100	0.4096406887794011
1102	1	1.557018431448416
1102	2	3.0492310117786907
1102	3	3.139789614042563
1102	4	1.3073283694207518
1102	5	2.5611431116378967
1102	6	1.1336742426099562
1102	7	0.6355257307030868
1102	8	0.46891056455427105
1102	9	0.3767967945411712
1102	10-11	0.4608173939797453
1102	12-13	0.45041440490891205
1102	14-15	0.5385450434941887
1102	16-17	0.4777104635866323
1102	18-19	0.29128674149166756
1102	20-21	0.23038437926496158
1102	22-23	0.22925314207894587
1102	24-25	0.32219930169497957
1102	26-27	0.3248441359118601
1102	28-29	0.4001468043565737
1102	30-31	0.4160389863189806
1102	32-33	0.38235433365702676
1102	34-35	0.3851553488510362
1102	36-37	0.42318722449342516
1102	38-39	0.5496864319857764
1102	40-41	0.5683362260097908
1102	42-43	0.5838956038280045
1102	44-45	0.6135103781760165
1102	46-47	0.6592086705056559
1102	48-49	0.5603942117707348
1102	50-51	0.5677907793247883
1102	52-53	0.5628554255760676
1102	54-55	0.6516195695723184
1102	56-57	0.5735723536542565
1102	58-59	0.5994176543745979
1102	60-61	0.5565856894001371
1102	62-63	0.5818464261592311
1102	64-65	0.5959954476730047
1102	66-67	0.5778401680827088
1102	68-69	0.5582718126846125
1102	70-71	0.6299568668506836
1102	72-73	0.6548268626858338
1102	74-75	0.6583588382594812
1102	76-77	0.6423802903752289
1102	78-79	0.6403689670425514
1102	80-81	0.6286133308490172
1102	82-83	0.6201485473661812
1102	84-85	0.5698127323220916
1102	86-87	0.6010554029009612
1102	88-89	0.560803592147014
1102	90-91	0.6432118378019567
1102	92-93	0.6982292903587037
1102	94-95	0.6609492059918232
1102	96-97	0.758478613412013
1102	98-99	0.8742527399502613
1102	100	0.8647131650493449
1103	1	1.3242870259200288
1103	2	2.9417830014667565
1103	3	3.1426186829436062
1103	4	1.3578166673199235
1103	5	2.5101412651005646
1103	6	1.1485436375954237
1103	7	0.6476458957744597
1103	8	0.4551586309241742
1103	9	0.36910200400662063
1103	10-11	0.38916910956027806
1103	12-13	0.4056189965799035
1103	14-15	0.4290565402134874
1103	16-17	0.2983983096464442
1103	18-19	0.24041010562211795
1103	20-21	0.18234605730978615
1103	22-23	0.21135384805661772
1103	24-25	0.3106790194632083
1103	26-27	0.38192649883485785
1103	28-29	0.44683070815306536
1103	30-31	0.44876510085511967
1103	32-33	0.3948961187467859
1103	34-35	0.4938914976875992
1103	36-37	0.5201058283617641
1103	38-39	0.5267782845593842
1103	40-41	0.5586151656039249
1103	42-43	0.5351007553201086
1103	44-45	0.6738299180758816
1103	46-47	0.6075302786335328
1103	48-49	0.6877356417761291
1103	50-51	0.6935416403437031
1103	52-53	0.7125467464333965
1103	54-55	0.7725729324151018
1103	56-57	0.7479047167194892
1103	58-59	0.7018808483171455
1103	60-61	0.6816065736482102
1103	62-63	0.6781785168995569
1103	64-65	0.7060637318155898
1103	66-67	0.7813036155756734
1103	68-69	0.6903298114417034
1103	70-71	0.7131292737382253
1103	72-73	0.750692909933445
1103	74-75	0.74509446292749
1103	76-77	0.7200860023521969
1103	78-79	0.6715950189022415
1103	80-81	0.6605522917626168
1103	82-83	0.6721349641184844
1103	84-85	0.7116529982143618
1103	86-87	0.7211520137644101
1103	88-89	0.7103258477861409
1103	90-91	0.6987263412840541
1103	92-93	0.712578783722492
1103	94-95	0.6753188487436468
1103	96-97	0.7035468776422675
1103	98-99	0.7573497359751897
1103	100	0.7319308787603909
1104	1	5.949803138545512
1104	2	4.992630339321355
1104	3	4.003776012334832
1104	4	1.733776919007596
1104	5	2.740596806133432
1104	6	1.2273068064402182
1104	7	0.6583353350570391
1104	8	0.4696962353099323
1104	9	0.37739789212047015
1104	10-11	0.39209173639665096
1104	12-13	0.29699645815836107
1104	14-15	0.3130674146686516
1104	16-17	0.18330783265972173
1104	18-19	0.0867410645238138
1104	20-21	0.06986521278571445
1104	22-23	0.04537083464351355
1104	24-25	0.053033348366746225
1104	26-27	0.15622304845389579
1104	28-29	0.1505940376019197
1104	30-31	0.22548177014345328
1104	32-33	0.24327693612418244
1104	34-35	0.21295442896686012
1104	36-37	0.19707237003870404
1104	38-39	0.25247962414410097
1104	40-41	0.24612944789373614
1104	42-43	0.3491530509395133
1104	44-45	0.36680235236174497
1104	46-47	0.3346290112196897
1104	48-49	0.36140530326498066
1104	50-51	0.3893291885180332
1104	52-53	0.33303150088631384
1104	54-55	0.42123287047516556
1104	56-57	0.5081417114155187
1104	58-59	0.48708493753518667
1104	60-61	0.4462069512788389
1104	62-63	0.4527632819798697
1104	64-65	0.429463064547015
1104	66-67	0.37097863738570425
1104	68-69	0.3483840776270597
1104	70-71	0.33301726938301
1104	72-73	0.3897094917981079
1104	74-75	0.4336891058000347
1104	76-77	0.31243067665725377
1104	78-79	0.29905104505115077
1104	80-81	0.2946764609119157
1104	82-83	0.39139314405046477
1104	84-85	0.3729994641265648
1104	86-87	0.3848008536249097
1104	88-89	0.44631860390052225
1104	90-91	0.4973373364033442
1104	92-93	0.529711419367306
1104	94-95	0.45863308701876804
1104	96-97	0.48149336106489926
1104	98-99	0.49953878035532284
1104	100	0.46444967723643416
1105	1	1.7050002045831292
1105	2	3.1057113634496254
1105	3	3.1711273335153294
1105	4	1.319584642655684
1105	5	2.514813164363737
1105	6	1.1323537369053156
1105	7	0.6570008364407087
1105	8	0.4364897898070055
1105	9	0.34303589620493113
1105	10-11	0.4059374730139069
1105	12-13	0.33962800165737406
1105	14-15	0.3886178147405914
1105	16-17	0.3022622180639871
1105	18-19	0.3200345300841647
1105	20-21	0.2915079232616762
1105	22-23	0.27001545049689213
1105	24-25	0.3090518027808997
1105	26-27	0.3423251051760019
1105	28-29	0.3674968149891029
1105	30-31	0.4397851860479207
1105	32-33	0.44046357121906254
1105	34-35	0.4594107529766802
1105	36-37	0.40679518661536207
1105	38-39	0.3577841486676192
1105	40-41	0.3445959208998701
1105	42-43	0.3777714185485692
1105	44-45	0.5178160966227381
1105	46-47	0.543461117142698
1105	48-49	0.47566914994201426
1105	50-51	0.49414357588196367
1105	52-53	0.46972562717574107
1105	54-55	0.3736236919190645
1105	56-57	0.4572185428894784
1105	58-59	0.4562587807890992
1105	60-61	0.506782127347563
1105	62-63	0.5176655922029667
1105	64-65	0.5268816874483377
1105	66-67	0.515887722204468
1105	68-69	0.47914067027413054
1105	70-71	0.4355640838631629
1105	72-73	0.5087061512525111
1105	74-75	0.5096645750285589
1105	76-77	0.473229385722405
1105	78-79	0.49168511424553074
1105	80-81	0.44741958999053466
1105	82-83	0.4602843512450754
1105	84-85	0.5219394960578043
1105	86-87	0.5981444605077684
1105	88-89	0.5140401544052047
1105	90-91	0.5196832022609748
1105	92-93	0.6129314695910253
1105	94-95	0.6416574227205842
1105	96-97	0.6364989578983611
1105	98-99	0.6102132287909221
1105	100	0.5465349558179433
1106	1	-0.7537612019341999
1106	2	1.8884229122542067
1106	3	2.6791815603070326
1106	4	1.1550580863967568
1106	5	2.4232701024950245
1106	6	1.0873874302523916
1106	7	0.5991415624826502
1106	8	0.38828581240125715
1106	9	0.31071077208910935
1106	10-11	0.45041698375786865
1106	12-13	0.36105144051064997
1106	14-15	0.4632072486541219
1106	16-17	0.3297054050675712
1106	18-19	0.22947984968308077
1106	20-21	0.17310784952985614
1106	22-23	0.08230909430224642
1106	24-25	0.0609825735128382
1106	26-27	0.05704123949342943
1106	28-29	0.040522550061744766
1106	30-31	0.08237878198531234
1106	32-33	0.1458997414356631
1106	34-35	0.19582258195494262
1106	36-37	0.20105204098673113
1106	38-39	0.28148822364897796
1106	40-41	0.2528733090765911
1106	42-43	0.2883429158635167
1106	44-45	0.2750182051217678
1106	46-47	0.3220583477266814
1106	48-49	0.3413060842424258
1106	50-51	0.3530142454454719
1106	52-53	0.37207876865965517
1106	54-55	0.4075126411288892
1106	56-57	0.2748695569363946
1106	58-59	0.3819144582650722
1106	60-61	0.3674004104568098
1106	62-63	0.4135983122828719
1106	64-65	0.4564857504897617
1106	66-67	0.4485732628689689
1106	68-69	0.355762887430199
1106	70-71	0.40699210390061324
1106	72-73	0.481742635875424
1106	74-75	0.4692353541436951
1106	76-77	0.3750083461110343
1106	78-79	0.46575534127386575
1106	80-81	0.47770861221071925
1106	82-83	0.47440540055990255
1106	84-85	0.38922222979108057
1106	86-87	0.4279652820980573
1106	88-89	0.4800162947322839
1106	90-91	0.47145325278746597
1106	92-93	0.43461771330497356
1106	94-95	0.3972817281030423
1106	96-97	0.3336607700128127
1106	98-99	0.319918647826789
1106	100	0.34155870763463625
1107	1	-0.985062797023911
1107	2	1.771349177687327
1107	3	-0.5765910981286382
1107	4	-0.030988629303628556
1107	5	1.8039450316100059
1107	6	0.7591320271867588
1107	7	0.33705468883017176
1107	8	0.2891672869982429
1107	9	0.2425139641556484
1107	10-11	0.24612500827157646
1107	12-13	0.20906340486291697
1107	14-15	0.32627547282532277
1107	16-17	0.2677250594412115
1107	18-19	0.15772968573937618
1107	20-21	0.10931813151303515
1107	22-23	0.06275917285368848
1107	24-25	-0.13985375169486503
1107	26-27	-0.2549623177311773
1107	28-29	-0.2736903078427062
1107	30-31	-0.23728531033268752
1107	32-33	-0.21986423623968676
1107	34-35	-0.18606119021283973
1107	36-37	-0.19837644417341949
1107	38-39	-0.31950242094465153
1107	40-41	-0.31706743529860404
1107	42-43	-0.20399404860669534
1107	44-45	-0.17701606501028522
1107	46-47	-0.12983141605284487
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2216	82-83	0.5437583064916005
2216	84-85	0.5673228888683752
2216	86-87	0.49582028509386333
2216	88-89	0.3411606925753041
2216	90-91	0.30967973571137364
2216	92-93	0.29111861204673417
2216	94-95	0.2106430222911797
2216	96-97	0.18986208816739492
2216	98-99	0.14421463344692143
2216	100	0.13125313543571693
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	828.0
3	246.0
4	356.0
5	1320.0
6	4433.0
7	7775.0
8	9568.0
9	13060.0
10	8362.0
11	4303.0
12	4148.0
13	5330.0
14	5846.0
15	6624.0
16	7511.0
17	8406.0
18	11419.0
19	20624.0
20	40598.0
21	45947.0
22	19968.0
23	12406.0
24	12589.0
25	13331.0
26	14591.0
27	16215.0
28	19826.0
29	23429.0
30	27065.0
31	33662.0
32	44762.0
33	65714.0
34	98358.0
35	234461.0
36	266093.0
37	397171.0
38	352665.0
39	85871.0
40	432.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.424180996630403	43.36123656871536	24.092921788923057	19.121660645731176
2	14.53282856843218	42.631539443840104	24.15293882981458	18.682693157913135
3	18.222990586847907	27.03375440880048	18.254348911223556	36.48890609312805
4	32.27300696597412	28.90542550221995	20.413681500097926	18.407886031708006
5	16.72522766820973	41.3675257612716	21.17742312744022	20.72982344307845
6	36.39451200639985	25.26829699657489	22.61134183973027	15.725849157294993
7	18.732219435936507	25.011643795476346	38.62336922603974	17.632767542547406
8	35.446429443488014	25.41380230327973	21.982220855975363	17.157547397256895
9	34.610317208593166	27.245435567438246	22.25461917953563	15.889628044432952
10-11	16.10704806887118	44.98011374005109	22.115798331682356	16.797039859395376
12-13	15.974909950223948	26.885699113715887	30.882639451851706	26.25675148420845
14-15	16.287353243411214	35.4888647739464	22.055910796874333	26.16787118576805
16-17	25.694348415910444	26.203366758974006	31.022154275430225	17.08013054968532
18-19	16.648554756997974	26.539842174498396	31.271471480425	25.54013158807863
20-21	25.799986942975245	35.82670243811664	22.124511582454854	16.24879903645326
22-23	25.166567025460683	26.836658162465373	31.360146156428296	16.636628655645648
24-25	16.4283074240495	35.815496015294194	21.778783157260552	25.97741340339575
26-27	17.14796465863454	30.730461044176703	35.20377831325302	16.917795983935743
28-29	25.444619966041454	26.72996582041039	31.041328567690652	16.784085645857505
30-31	22.74464314997124	28.305727664391284	31.358449771322146	17.59117941431533
32-33	18.866372660852008	29.72966304137175	32.3018712155833	19.10209308219294
34-35	19.02896860299602	29.68524859495618	32.27624551935858	19.009537282689212
36-37	19.437540385531786	29.368410122175714	32.699545008952285	18.49450448334021
38-39	20.1476317692834	28.84366166267331	32.22558529141583	18.783121276627462
40-41	18.2960531287253	30.659898946853282	32.31168968695526	18.73235823746616
42-43	18.29586477132675	29.374658631905916	33.15351507931306	19.175961517454272
44-45	18.584027351896584	30.103561740449997	31.84171390413779	19.47069700351563
46-47	19.421990188725413	29.05401341583591	32.69311930779263	18.83087708764605
48-49	18.556859487393545	29.381235821690392	32.63492815809075	19.426976532825307
50-51	19.208245665350514	30.28330145328798	31.576563771485617	18.93188910987589
52-53	19.52488794199586	29.59559905506039	31.736518019369154	19.142994983574592
54-55	19.53685602265548	30.633836302949707	29.61289519989842	20.216412474496394
56-57	19.845752328802615	31.236181529656154	28.65623681124837	20.261829330292862
58-59	21.021578532606323	31.259314053830927	27.469075670599025	20.250031742963728
60-61	20.648605134495064	31.859705867384836	27.09145006484818	20.400238933271922
62-63	19.652585202935366	31.843563357333775	26.7953744168993	21.708477022831563
64-65	20.28699237603409	31.887053651520347	27.748002506537507	20.077951465908058
66-67	20.54597383557299	31.007786407637226	28.278559799888242	20.167679956901537
68-69	20.850680584564028	31.331693151693322	27.830020156139394	19.987606107603252
70-71	18.95263127321927	32.672325738839966	28.179526893615574	20.195516094325182
72-73	19.129646931518362	31.737840258777233	28.392627881781884	20.73988492792252
74-75	19.364381958907696	32.28858633113034	27.37696200104508	20.970069708916885
76-77	20.471161260473963	31.203721790983398	27.80455456741891	20.52056238112373
78-79	20.19011948267185	31.66288233761013	27.139293981451733	21.007704198266286
80-81	20.650031614823497	32.55391227014995	26.777858314184517	20.01819780084203
82-83	20.21404045111093	32.62447459538736	26.769373046078304	20.392111907423406
84-85	20.227462989327776	32.84513542627591	25.977396096764306	20.95000548763201
86-87	20.030642899442352	32.86272330608149	26.48474897599974	20.621884818476417
88-89	21.729485177963646	31.998552418042753	26.152809342249807	20.11915306174379
90-91	20.583428112449802	32.79252048192771	26.23545060240964	20.38860080321285
92-93	19.774838290804617	32.97407666529757	26.324401266017343	20.926683777880477
94-95	20.073561445783135	33.19862489959839	26.12467148594377	20.603142168674697
96-97	20.22834729660088	32.16872099553664	26.565781576711867	21.037150131150618
98-99	21.254451764667177	32.24085177782713	25.513957773007295	20.9907386844984
100	19.885334647946117	33.45322834937103	25.42521434853928	21.236222654143575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	128732.0
1	69094.0
2	6675.5
3	3230.5
4	2438.0
5	2173.5
6	2113.5
7	2327.0
8	2235.5
9	1974.0
10	2038.5
11	3198.0
12	21702.0
13	23480.5
14	6381.0
15	4981.0
16	5291.5
17	5971.0
18	6564.5
19	7139.5
20	7956.5
21	8810.0
22	9765.5
23	10892.5
24	12158.0
25	13563.0
26	15172.0
27	16989.0
28	18667.0
29	20448.5
30	22434.0
31	24221.5
32	26419.5
33	29142.5
34	32098.5
35	35151.5
36	37875.0
37	40319.0
38	41797.5
39	42303.0
40	42346.5
41	42442.0
42	42694.0
43	43708.0
44	46691.5
45	49360.0
46	51645.0
47	55288.0
48	58247.5
49	65210.5
50	86985.5
51	90435.5
52	69356.0
53	60166.0
54	60421.0
55	62862.5
56	58378.0
57	49026.0
58	41427.5
59	33228.5
60	27228.0
61	23140.0
62	18947.5
63	14291.5
64	10313.5
65	7644.0
66	5969.5
67	4642.0
68	3659.5
69	2740.5
70	1937.0
71	1448.5
72	1136.5
73	852.0
74	614.5
75	438.5
76	319.5
77	217.0
78	128.5
79	68.5
80	40.0
81	25.5
82	15.5
83	8.0
84	2.5
85	1.5
86	1.0
87	1.5
88	0.5
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13607064775694194
2	0.006014456285440955
3	0.0028787141195272944
4	0.0
5	0.0024674692453091097
6	0.013725297677031923
7	0.003649798258686391
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	2.570280463863656E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.967869437977333E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.799196324704559E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	7.710841391590968E-5
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	5.397588974113678E-4
74-75	4.883532881340946E-4
76-77	6.682729206045505E-4
78-79	5.140560927727312E-4
80-81	0.0011823290133772817
82-83	0.0013622486458477376
84-85	0.001516465473679557
86-87	5.140560927727312E-5
88-89	0.0
90-91	2.570280463863656E-5
92-93	0.0
94-95	2.570280463863656E-5
96-97	0.00156787108295683
98-99	8.73895357713643E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1945313.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.243165000592256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.02044518355261	51.26589312251241
2	8.269698975905326	9.633068839177653
3	1.8796869238147158	3.2843674695958858
4	0.6193013956033105	1.442802934768827
5	0.2685020357618347	0.781920418593573
6	0.14840400707605741	0.5186111442527923
7	0.09674542801973005	0.394433194904424
8	0.06604789769616626	0.30774708827680386
9	0.05072938214113692	0.2659175796981902
>10	0.4846723808053308	6.2952583577547445
>50	0.05334420747225879	2.1845455478750195
>100	0.036059283126686735	3.8071773450737623
>500	0.0030930759148330694	1.2543624106366602
>1k	0.0027395815245664333	3.0245729985158567
>5k	1.7674719513331827E-4	0.7981697949012182
>10k+	3.5349439026663654E-4	14.741151753462276
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	137903	7.088987736163794	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	119429	6.139320510375451	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	18119	0.9314182344949117	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10943	0.5625315823211997	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9333	0.47976855138479	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	6174	0.3173782316778842	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4560	0.23440957830436543	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4358	0.22402564523035626	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3856	0.19822002937316513	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	3661	0.1881959355640969	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3230	0.16604011796559218	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2958	0.1520577922421739	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2955	0.15190357541434207	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2392	0.12296221739123729	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2376	0.12213972764280093	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0029301197288045676	0.0	0.0	0.0	0.0
2	0.0029301197288045676	0.0	0.0	0.0	0.0
3	0.0029301197288045676	0.0	0.0	0.0	0.0
4	0.0029301197288045676	0.0	0.0	0.0	0.0
5	0.0029301197288045676	5.140560927727312E-5	0.0	0.0	0.0
6	0.0029301197288045676	5.140560927727312E-5	0.0	0.0	0.0
7	0.0029301197288045676	5.140560927727312E-5	0.0	0.0	0.0
8	0.0029301197288045676	5.140560927727312E-5	0.0	0.0	0.0
9	0.003032930947359114	5.140560927727312E-5	0.0	0.0	0.0
10-11	0.003084336556636387	5.140560927727312E-5	0.0	0.0	0.0
12-13	0.003315661798384116	7.710841391590968E-5	0.0	0.0	0.0
14-15	0.0033927702123000255	1.0281121855454623E-4	0.0	0.0	0.0
16-17	0.003495581430854572	1.0281121855454623E-4	0.0	0.0	0.0
18-19	0.0035212842354932087	1.0281121855454623E-4	0.0	0.0	0.0
20-21	0.003624095454047755	1.5421682783181936E-4	0.0	0.0	0.0
22-23	0.003829717891156847	1.5421682783181936E-4	0.0	0.0	0.0
24-25	0.003932529109711393	1.5421682783181936E-4	0.0	0.0	0.0
26-27	0.004009637523627303	2.0562243710909247E-4	0.0	0.0	0.0
28-29	0.004009637523627303	2.0562243710909247E-4	0.0	0.0	2.570280463863656E-5
30-31	0.0040867459375432125	2.0562243710909247E-4	0.0	0.0	5.140560927727312E-5
32-33	0.004163854351459122	2.0562243710909247E-4	0.0	0.0	5.140560927727312E-5
34-35	0.0046008020303159435	2.0562243710909247E-4	0.0	0.0	5.140560927727312E-5
36-37	0.005320480560197767	2.0562243710909247E-4	0.0	0.0	5.140560927727312E-5
38-39	0.0068369460338773246	2.0562243710909247E-4	0.0	0.0	5.140560927727312E-5
40-41	0.009972688199790984	2.0562243710909247E-4	0.0	0.0	5.140560927727312E-5
42-43	0.013031321951788735	2.0562243710909247E-4	0.0	0.0	5.140560927727312E-5
44-45	0.016269875336256942	2.0562243710909247E-4	0.0	0.0	7.710841391590968E-5
46-47	0.019508428720725147	2.570280463863656E-4	0.0	0.0	1.0281121855454623E-4
48-49	0.022361440035613807	3.084336556636387E-4	0.0	0.0	1.0281121855454623E-4
50-51	0.027065053284484296	3.084336556636387E-4	0.0	0.0	1.0281121855454623E-4
52-53	0.03328513200703434	3.598392649409118E-4	0.0	0.0	1.0281121855454623E-4
54-55	0.04068753974296167	3.598392649409118E-4	0.0	0.0	1.0281121855454623E-4
56-57	0.05012046904534129	4.1124487421818494E-4	0.0	0.0	1.0281121855454623E-4
58-59	0.05888512542711635	4.1124487421818494E-4	0.0	0.0	1.0281121855454623E-4
60-61	0.07147949970004827	4.1124487421818494E-4	0.0	0.0	1.0281121855454623E-4
62-63	0.08828913393371658	4.369476788568215E-4	0.0	0.0	1.285140231931828E-4
64-65	0.10818310472402129	4.626504834954581E-4	0.0	0.0	1.5421682783181936E-4
66-67	0.131752576577651	4.626504834954581E-4	0.0	0.0	1.5421682783181936E-4
68-69	0.16426662444552625	4.626504834954581E-4	0.0	0.0	1.5421682783181936E-4
70-71	0.20672765770855384	4.626504834954581E-4	0.0	0.0	1.5421682783181936E-4
72-73	0.2551003360384678	4.626504834954581E-4	0.0	0.0	1.5421682783181936E-4
74-75	0.3064802425111023	5.654617020500042E-4	0.0	0.0	1.5421682783181936E-4
76-77	0.3673701867000323	5.911645066886408E-4	0.0	0.0	1.5421682783181936E-4
78-79	0.43725611251248514	6.42570115965914E-4	0.0	0.0	1.5421682783181936E-4
80-81	0.5272673343569905	6.682729206045505E-4	0.0	0.0	1.5421682783181936E-4
82-83	0.6404110803762686	6.682729206045505E-4	0.0	0.0	1.5421682783181936E-4
84-85	0.7704672718477694	6.682729206045505E-4	0.0	0.0	1.5421682783181936E-4
86-87	0.9285138175707457	6.682729206045505E-4	0.0	0.0	1.5421682783181936E-4
88	1.0588013342839944	6.682729206045505E-4	0.0	0.0	1.5421682783181936E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2695	0.0	85.76064	1
AACGTGT	1260	0.0	73.22356	1
ACGTGTG	1400	0.0	68.83867	2
ATCGAGT	1000	0.0	66.84264	1
TCGAGTG	1085	0.0	65.42653	2
CGTGTGG	1565	0.0	64.28282	3
GTGGAAT	52660	0.0	62.542263	6
TGGAATC	52920	0.0	62.241554	7
GGAATCA	53660	0.0	61.397568	8
GAATCAC	53690	0.0	61.389526	9
AACGAGT	45945	0.0	60.140244	1
ACGAGTG	49920	0.0	60.12083	2
CGAGTGG	51650	0.0	60.117096	3
GAGTGGA	53410	0.0	59.780483	4
AGTGGAA	53700	0.0	59.431385	5
AGCGAGT	675	0.0	57.184113	1
ATGAGTG	1655	0.0	53.403107	2
TGAGTGG	1840	0.0	53.14238	3
GCGAGTG	795	0.0	52.629536	2
AATGAGT	1680	0.0	47.63267	1
>>END_MODULE