FastQCFastQC Report
Thu 26 May 2016
SRR2132790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132790_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024532
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1692228.358573734571744No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250051.2351002602082852No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG148960.7357749840456955No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT137380.6785765796737221No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55720.27522410117498763No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46260.22849725269840143No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42020.20755414090762705No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39510.1951562138805413No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC28440.14047691021925068No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23020.11370529090179854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT34650.089.393731
AACGTGT16150.075.202381
ATCGAGT10900.074.7143551
CGTGTGG18300.073.976273
GTGGAAT593050.071.0901266
TGGAATC595550.070.7504967
AACGAGT502600.070.667741
ACGAGTG548600.070.532742
ACGTGTG17750.070.449392
TCGAGTG12100.070.321342
GAATCAC599850.070.194579
GGAATCA600650.070.124558
CGAGTGG568800.070.1202243
AGTGGAA585200.069.703675
GAGTGGA592400.068.935844
AGCGAGT6800.060.227491
GCGAGTG7250.058.3577352
ATGAGTG20300.052.568282
GTGTGGA27550.051.5259064
TGAGTGG23000.050.2757233