##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132790_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2024532 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.904708841351976 31.0 25.0 33.0 16.0 34.0 2 28.202480375711524 31.0 25.0 34.0 16.0 34.0 3 29.284844596183216 31.0 28.0 34.0 16.0 34.0 4 34.759424400305846 35.0 35.0 37.0 32.0 37.0 5 33.84437687327244 35.0 35.0 37.0 28.0 37.0 6 35.26216775037391 37.0 35.0 37.0 32.0 37.0 7 35.62946103099383 37.0 35.0 37.0 33.0 37.0 8 35.91354841513989 37.0 35.0 37.0 35.0 37.0 9 37.879313836481714 39.0 38.0 39.0 35.0 39.0 10-11 37.88200137118109 39.0 38.0 39.0 35.0 39.0 12-13 37.87658036523997 39.0 38.0 39.0 35.0 39.0 14-15 39.313765107195145 41.0 39.0 41.0 36.0 41.0 16-17 39.31574457701829 41.0 39.0 41.0 36.0 41.0 18-19 39.26143054296005 41.0 39.0 41.0 36.0 41.0 20-21 39.215174420557446 40.5 39.0 41.0 36.0 41.0 22-23 39.09807624675727 40.0 39.0 41.0 36.0 41.0 24-25 39.010600474578816 40.0 39.0 41.0 35.0 41.0 26-27 38.84839928437782 40.0 39.0 41.0 35.0 41.0 28-29 38.65722201476687 40.0 39.0 41.0 35.0 41.0 30-31 38.492962077161536 40.0 38.5 41.0 34.5 41.0 32-33 38.343624106707125 40.0 38.0 41.0 34.0 41.0 34-35 38.25435755028816 40.0 38.0 41.0 34.0 41.0 36-37 38.07230016616185 40.0 38.0 41.0 34.0 41.0 38-39 37.538989998676236 40.0 36.0 41.0 33.5 41.0 40-41 37.27950583147118 40.0 35.0 41.0 33.0 41.0 42-43 37.1200324321868 40.0 35.0 41.0 33.0 41.0 44-45 36.96829440087882 40.0 35.0 41.0 33.0 41.0 46-47 36.83539183376701 40.0 35.0 41.0 33.0 41.0 48-49 36.6694636093675 39.5 35.0 41.0 32.0 41.0 50-51 36.36128423754231 39.0 35.0 41.0 31.0 41.0 52-53 35.83799465753073 39.0 35.0 41.0 29.0 41.0 54-55 34.8143679625711 39.0 35.0 41.0 22.0 41.0 56-57 33.96368741022617 39.0 35.0 41.0 9.0 41.0 58-59 33.57067090073163 39.0 34.0 41.0 2.0 41.0 60-61 33.31884702242296 38.0 34.0 40.5 2.0 41.0 62-63 33.052135999826135 38.0 34.0 40.0 2.0 41.0 64-65 32.76763987924123 37.0 34.0 40.0 2.0 41.0 66-67 32.45013094384282 37.0 33.5 40.0 2.0 41.0 68-69 32.128434867910215 36.0 33.0 39.5 2.0 41.0 70-71 31.789912187112876 36.0 33.0 39.0 2.0 41.0 72-73 31.450896552882348 35.0 33.0 39.0 2.0 40.5 74-75 31.09294913589906 35.0 33.0 38.0 2.0 40.0 76-77 30.73804069286136 35.0 32.5 37.0 2.0 39.5 78-79 30.376843882931958 35.0 32.0 37.0 2.0 39.0 80-81 30.037903327781432 35.0 32.0 36.0 2.0 39.0 82-83 29.712088275216196 35.0 32.0 36.0 2.0 37.5 84-85 29.41011255934705 35.0 31.0 35.5 2.0 37.0 86-87 29.180908229655053 35.0 31.0 35.0 2.0 37.0 88-89 28.939618143847568 35.0 31.0 35.0 2.0 36.0 90-91 28.723074024021354 35.0 31.0 35.0 2.0 36.0 92-93 28.533058010443895 35.0 31.0 35.0 2.0 36.0 94-95 28.323861514661168 35.0 31.0 35.0 2.0 35.5 96-97 28.116426413610654 34.0 30.0 35.0 2.0 35.0 98-99 27.940571697557758 34.0 30.0 35.0 2.0 35.0 100 27.833053268607262 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2296638846720569 1101 2 2.315751616032781 1101 3 1.3019712094306293 1101 4 0.6072063097639244 1101 5 2.282448834640171 1101 6 0.945274255125149 1101 7 0.5178544427220686 1101 8 0.3599403973362456 1101 9 0.2798496798874339 1101 10-11 0.43337100507478965 1101 12-13 0.33482435947762923 1101 14-15 0.4610856010589117 1101 16-17 0.40812930009782633 1101 18-19 0.324436889476452 1101 20-21 0.295727632043679 1101 22-23 0.30185926409384933 1101 24-25 0.32192654498734896 1101 26-27 0.22451445703991624 1101 28-29 0.3324003611806674 1101 30-31 0.41518440736723505 1101 32-33 0.32931893228585807 1101 34-35 0.2876402502775406 1101 36-37 0.29478538900252005 1101 38-39 0.3453019648520481 1101 40-41 0.41061060143691464 1101 42-43 0.4388626472213204 1101 44-45 0.4623345592601211 1101 46-47 0.3860470461121608 1101 48-49 0.4416598289508826 1101 50-51 0.499591310642181 1101 52-53 0.552186363689465 1101 54-55 0.688103707881595 1101 56-57 0.6579884562653362 1101 58-59 0.732619551816029 1101 60-61 0.7176152439914105 1101 62-63 0.668495010037077 1101 64-65 0.6828661088125827 1101 66-67 0.504364166377286 1101 68-69 0.6292947417623083 1101 70-71 0.5552730021771133 1101 72-73 0.6240998674879847 1101 74-75 0.5347079008299715 1101 76-77 0.5486127686561986 1101 78-79 0.5552742382840492 1101 80-81 0.6479840681064459 1101 82-83 0.5842053294697465 1101 84-85 0.5765173549670912 1101 86-87 0.6454027889544918 1101 88-89 0.5746965546590914 1101 90-91 0.5919399502973768 1101 92-93 0.6093961482525536 1101 94-95 0.7432516189577676 1101 96-97 0.7093764777794149 1101 98-99 0.6299616391680765 1101 100 0.7120813150790539 1102 1 1.606013718135312 1102 2 3.072047590804239 1102 3 3.215277229556772 1102 4 1.3982267902401517 1102 5 2.679459942995358 1102 6 1.2316713921104778 1102 7 0.7099667132911307 1102 8 0.5316365032110895 1102 9 0.4175548389831718 1102 10-11 0.4807641292332434 1102 12-13 0.48216486511152823 1102 14-15 0.6218056510199332 1102 16-17 0.5281482569399998 1102 18-19 0.44758660423797636 1102 20-21 0.44725303775553726 1102 22-23 0.3559212104477609 1102 24-25 0.3706992666229496 1102 26-27 0.4442127863574683 1102 28-29 0.526708037815915 1102 30-31 0.5061972253815412 1102 32-33 0.5091670117982972 1102 34-35 0.547416267214679 1102 36-37 0.5584231981585006 1102 38-39 0.6686515237068491 1102 40-41 0.7334989509514713 1102 42-43 0.6988932084855861 1102 44-45 0.8124543239826849 1102 46-47 0.8527187472672892 1102 48-49 0.7226497481628442 1102 50-51 0.8145836924719561 1102 52-53 0.9476081534279643 1102 54-55 0.9758820368448085 1102 56-57 0.9586735600850531 1102 58-59 0.9289403299323951 1102 60-61 0.9122249013509176 1102 62-63 0.9028062300073074 1102 64-65 0.9217030091031404 1102 66-67 0.9173819362022471 1102 68-69 0.8916817536679176 1102 70-71 0.9291009112551372 1102 72-73 0.8940065891944613 1102 74-75 0.8546547950968524 1102 76-77 0.9087145952453852 1102 78-79 0.9167974294115382 1102 80-81 0.9017567454503634 1102 82-83 0.821496006955396 1102 84-85 0.8296054156998487 1102 86-87 0.7798814757945465 1102 88-89 0.7967485885333758 1102 90-91 0.8110058829031423 1102 92-93 0.9014783890575977 1102 94-95 0.8870043650423831 1102 96-97 0.9551183192506052 1102 98-99 0.8727787676494039 1102 100 0.738036686774894 1103 1 1.2036396241561462 1103 2 2.790778096983491 1103 3 3.121977567690987 1103 4 1.3579171887615544 1103 5 2.629302203810113 1103 6 1.2365760416688616 1103 7 0.7490986367382249 1103 8 0.4717732048268104 1103 9 0.4202404934048971 1103 10-11 0.5079154123546417 1103 12-13 0.4528619581793478 1103 14-15 0.6210406330059826 1103 16-17 0.4466449224426441 1103 18-19 0.4502045892306157 1103 20-21 0.4580799334790342 1103 22-23 0.3895375856830441 1103 24-25 0.46184845450463285 1103 26-27 0.5029264284121737 1103 28-29 0.4743752524246716 1103 30-31 0.5492691789796567 1103 32-33 0.6082401663264818 1103 34-35 0.6221681175838967 1103 36-37 0.5289019519167297 1103 38-39 0.533612855461655 1103 40-41 0.6367019847904487 1103 42-43 0.6514186537240292 1103 44-45 0.7495523318015032 1103 46-47 0.7203285131285142 1103 48-49 0.8075568251330978 1103 50-51 0.7366021919055541 1103 52-53 0.7970308978513074 1103 54-55 0.7328324409875933 1103 56-57 0.5775309730602132 1103 58-59 0.6823701777294104 1103 60-61 0.8608961275771847 1103 62-63 0.6790252592129136 1103 64-65 0.7006317113321003 1103 66-67 0.7639790880389938 1103 68-69 0.7922497680450107 1103 70-71 0.7648932695922319 1103 72-73 0.7592925553469669 1103 74-75 0.7978791646085597 1103 76-77 0.6803822271986419 1103 78-79 0.6972415180248461 1103 80-81 0.7076062118456115 1103 82-83 0.7281919587137793 1103 84-85 0.7480857706981894 1103 86-87 0.8272529294309798 1103 88-89 0.7785393371131235 1103 90-91 0.8294384988014158 1103 92-93 0.8822299498408448 1103 94-95 0.8454879140037086 1103 96-97 0.8391850006804802 1103 98-99 0.8588837936695519 1103 100 0.8761913427212029 1104 1 6.0339440493724155 1104 2 5.093176877375164 1104 3 4.110562798025224 1104 4 1.86767485016032 1104 5 2.9113378461141366 1104 6 1.3125591554331208 1104 7 0.7708330069786911 1104 8 0.583220365675551 1104 9 0.4752422467784001 1104 10-11 0.48158249165610556 1104 12-13 0.427956471661183 1104 14-15 0.5605447056660395 1104 16-17 0.4958370649288213 1104 18-19 0.4184980896810444 1104 20-21 0.4374959206120863 1104 22-23 0.45032883724137207 1104 24-25 0.47629833640738184 1104 26-27 0.5297472848953859 1104 28-29 0.5009228843323754 1104 30-31 0.6353708879430116 1104 32-33 0.6054154670194976 1104 34-35 0.6241364050424352 1104 36-37 0.6948147166380139 1104 38-39 0.7171215991346642 1104 40-41 0.7048139356609511 1104 42-43 0.6566119481490134 1104 44-45 0.7540703908997628 1104 46-47 0.6986644156973867 1104 48-49 0.7385492102044182 1104 50-51 0.8374137881112702 1104 52-53 0.6949198831946575 1104 54-55 0.7375880408343463 1104 56-57 0.6982929103546383 1104 58-59 0.7776376831606555 1104 60-61 0.7996063972852809 1104 62-63 0.8261367020754804 1104 64-65 0.7965826026005587 1104 66-67 0.7850240710713337 1104 68-69 0.6542441079412384 1104 70-71 0.6739852735278298 1104 72-73 0.6773399428204527 1104 74-75 0.7032489804378415 1104 76-77 0.6712094967143685 1104 78-79 0.6447486620892136 1104 80-81 0.603706423710225 1104 82-83 0.5786028823117846 1104 84-85 0.6039278133183039 1104 86-87 0.6580639433415065 1104 88-89 0.6513064621872111 1104 90-91 0.7333638900826891 1104 92-93 0.7544403261138228 1104 94-95 0.7840700868827675 1104 96-97 0.7841759077925587 1104 98-99 0.7305121586138945 1104 100 0.646126355984233 1105 1 1.4745081816141337 1105 2 3.031348615350307 1105 3 3.1595688196721348 1105 4 1.363299038117404 1105 5 2.5634456589362316 1105 6 1.1688173940635735 1105 7 0.6749946855505939 1105 8 0.4934871412765389 1105 9 0.3590950511751103 1105 10-11 0.3831813296900748 1105 12-13 0.3841974056216486 1105 14-15 0.43500334372232174 1105 16-17 0.3985636615446708 1105 18-19 0.39839618597822124 1105 20-21 0.3491146826568041 1105 22-23 0.34816461875998783 1105 24-25 0.4225057532078438 1105 26-27 0.4816133524233166 1105 28-29 0.5340427697622161 1105 30-31 0.6163651830755725 1105 32-33 0.6500065519939398 1105 34-35 0.6138072664367513 1105 36-37 0.6522932181859744 1105 38-39 0.6259462007017405 1105 40-41 0.6952846652371889 1105 42-43 0.6844368882445977 1105 44-45 0.7211668138448104 1105 46-47 0.7288644926700769 1105 48-49 0.6673143707028615 1105 50-51 0.7064468683992615 1105 52-53 0.7403217108704041 1105 54-55 0.7812558554720184 1105 56-57 0.7967452534826052 1105 58-59 0.8291221496189038 1105 60-61 0.6889757744495526 1105 62-63 0.6878137610002426 1105 64-65 0.6762629011864973 1105 66-67 0.581866890768012 1105 68-69 0.6088167487851877 1105 70-71 0.687359513966296 1105 72-73 0.685968992160511 1105 74-75 0.7392384387732989 1105 76-77 0.6650443534260297 1105 78-79 0.691629306201186 1105 80-81 0.7547946321378838 1105 82-83 0.7453275858156516 1105 84-85 0.739206479969841 1105 86-87 0.8385173729758044 1105 88-89 0.7586348953675426 1105 90-91 0.7477747559024657 1105 92-93 0.7705906422313724 1105 94-95 0.8222561981357153 1105 96-97 0.68180956509201 1105 98-99 0.7926490442271863 1105 100 0.826679783916429 1106 1 -0.3641833538902155 1106 2 2.136078804896872 1106 3 2.7748334041771336 1106 4 1.2304841907741633 1106 5 2.483946720818139 1106 6 1.1302185645732976 1106 7 0.6141223373663252 1106 8 0.4599473380392709 1106 9 0.37698172641900385 1106 10-11 0.42753480154313905 1106 12-13 0.37715457338871516 1106 14-15 0.4343664713818427 1106 16-17 0.4081409824692628 1106 18-19 0.35578464343745964 1106 20-21 0.3731460203712018 1106 22-23 0.30494461275078777 1106 24-25 0.3046986549060904 1106 26-27 0.3371845729840217 1106 28-29 0.35027765367426156 1106 30-31 0.32368687166040644 1106 32-33 0.3263279015983329 1106 34-35 0.3975653525326521 1106 36-37 0.4332556786703563 1106 38-39 0.47068593691331984 1106 40-41 0.49381038923006315 1106 42-43 0.45668135605814797 1106 44-45 0.5817352673492806 1106 46-47 0.5809455950241897 1106 48-49 0.6507430101843212 1106 50-51 0.6553441226577021 1106 52-53 0.6829672477563591 1106 54-55 0.6430564836130443 1106 56-57 0.5318071623533669 1106 58-59 0.5232735172252134 1106 60-61 0.533903940128809 1106 62-63 0.501903534319915 1106 64-65 0.49436554805875943 1106 66-67 0.4979735656010291 1106 68-69 0.473009179249253 1106 70-71 0.4536190189007705 1106 72-73 0.4347702019611539 1106 74-75 0.4797421714101837 1106 76-77 0.4889902398213586 1106 78-79 0.4645767732212107 1106 80-81 0.44341028224843626 1106 82-83 0.5090619111803747 1106 84-85 0.5032955149798433 1106 86-87 0.49474177289054566 1106 88-89 0.4694476651715789 1106 90-91 0.48291129825062384 1106 92-93 0.5308431954988109 1106 94-95 0.5627246203101741 1106 96-97 0.5812151937081218 1106 98-99 0.4754004290393219 1106 100 0.39711324231299727 1107 1 -1.0763452136361806 1107 2 1.6185850268809894 1107 3 -0.6779707270846913 1107 4 -0.1068219444073435 1107 5 1.8634880910787501 1107 6 0.7261707146333194 1107 7 0.2947711190780993 1107 8 0.20718220013840494 1107 9 0.19793947117863553 1107 10-11 0.21855446097851683 1107 12-13 0.22480768166819587 1107 14-15 0.29060913893462015 1107 16-17 0.2595296547283681 1107 18-19 0.20399094883346436 1107 20-21 0.14198947728522882 1107 22-23 0.19567775575131918 1107 24-25 0.1383589108381642 1107 26-27 0.11342625722595301 1107 28-29 0.10941532397352205 1107 30-31 0.16554794001068274 1107 32-33 0.13577022872211586 1107 34-35 0.19210746875320694 1107 36-37 0.24603804146459396 1107 38-39 0.21062429349573364 1107 40-41 0.2152055377778197 1107 42-43 0.2813917879689569 1107 44-45 0.26329089900782066 1107 46-47 0.14926230249260897 1107 48-49 0.17746770349306473 1107 50-51 0.23556668998059394 1107 52-53 0.2698337751771689 1107 54-55 0.4631865519022824 1107 56-57 0.5827580917516499 1107 58-59 0.6263066920985736 1107 60-61 0.7059053506712516 1107 62-63 0.6457003687371454 1107 64-65 0.591439011550797 1107 66-67 0.6287613671214274 1107 68-69 0.6036071000554344 1107 70-71 0.5304305148217203 1107 72-73 0.5889641922365456 1107 74-75 0.599393638577773 1107 76-77 0.558812938704456 1107 78-79 0.5675660381794039 1107 80-81 0.4979226617928596 1107 82-83 0.58112011640074 1107 84-85 0.549435172245154 1107 86-87 0.5878062102672068 1107 88-89 0.5085561413110327 1107 90-91 0.42783266729057345 1107 92-93 0.5223975725883498 1107 94-95 0.5527292656062208 1107 96-97 0.5668598726401761 1107 98-99 0.5327805159775671 1107 100 0.4978666618612202 1108 1 -1.4624002774216542 1108 2 0.28497796564115063 1108 3 2.010358773334758 1108 4 0.910252602151715 1108 5 0.7227673586306054 1108 6 0.49214998797069853 1108 7 0.282803026278188 1108 8 0.15018387794221866 1108 9 0.19529982293888537 1108 10-11 0.29427765227708136 1108 12-13 0.3192110850064438 1108 14-15 0.3842694632135917 1108 16-17 0.2424153326478944 1108 18-19 0.25006112731707475 1108 20-21 0.2192991694950166 1108 22-23 0.23245366046362648 1108 24-25 0.2759514793243838 1108 26-27 0.2793525568007169 1108 28-29 0.2994875346246886 1108 30-31 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2214 54-55 -2.0035130789270283 2214 56-57 -1.8752197244843245 2214 58-59 -1.8258430671652661 2214 60-61 -1.812581992347397 2214 62-63 -1.8928898057075223 2214 64-65 -1.9072594627392476 2214 66-67 -2.0219189377691364 2214 68-69 -2.011681427158859 2214 70-71 -1.9841890214730746 2214 72-73 -1.9533885061455543 2214 74-75 -1.9802028716907003 2214 76-77 -1.9727708496320986 2214 78-79 -1.911047727990148 2214 80-81 -1.9689299171639938 2214 82-83 -1.9673610877410113 2214 84-85 -1.9547102458060799 2214 86-87 -1.9365586359675468 2214 88-89 -1.9309881780278957 2214 90-91 -2.0092685676084194 2214 92-93 -2.1080003439648287 2214 94-95 -2.0597498349749834 2214 96-97 -2.025718057573574 2214 98-99 -1.9811601820525482 2214 100 -1.9901985652772254 2215 1 -2.1418659204742525 2215 2 -0.9695163926575567 2215 3 -3.3646002652112337 2215 4 -1.5286447323977939 2215 5 -3.497053762277474 2215 6 -1.4761868080834901 2215 7 -0.8029111387837986 2215 8 -0.5204108654786097 2215 9 -0.43263504412641396 2215 10-11 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-0.9265600484537586 2215 76-77 -0.8339197776809577 2215 78-79 -0.8483796927363869 2215 80-81 -0.8627961104528588 2215 82-83 -0.9604799003489788 2215 84-85 -1.0241965852189416 2215 86-87 -0.9901626771328189 2215 88-89 -1.0015565005381362 2215 90-91 -0.9233366723518195 2215 92-93 -1.0001810463974579 2215 94-95 -0.9352109379028484 2215 96-97 -0.9222161710765846 2215 98-99 -0.9301858589648582 2215 100 -0.8554016267364126 2216 1 2.8536452635825995 2216 2 -1.7962097111628772 2216 3 -2.1720602306079435 2216 4 -0.8309962679447196 2216 5 -2.54070848178203 2216 6 -1.0566478339036607 2216 7 -0.5744019742858697 2216 8 -0.46128119822006397 2216 9 -0.36367186128502027 2216 10-11 -0.2518827461915336 2216 12-13 -0.294422451031231 2216 14-15 -0.3351217079733999 2216 16-17 -0.44598475246080227 2216 18-19 -0.43377382608020554 2216 20-21 -0.4103007250473141 2216 22-23 -0.37340692443014945 2216 24-25 -0.3913261028176436 2216 26-27 -0.4426583663144328 2216 28-29 -0.39908119171516887 2216 30-31 -0.360373628026764 2216 32-33 -0.47537890369587643 2216 34-35 -0.5196812210855626 2216 36-37 -0.4630714368887894 2216 38-39 -0.41447712943433856 2216 40-41 -0.4052891069852649 2216 42-43 -0.33882513850915785 2216 44-45 -0.3751624632596702 2216 46-47 -0.4234644874982081 2216 48-49 -0.4094402423651431 2216 50-51 -0.368689090506912 2216 52-53 -0.3865616220272372 2216 54-55 -0.15345990687981725 2216 56-57 -0.16110325442976858 2216 58-59 -0.049955695924673194 2216 60-61 -0.047316774395781636 2216 62-63 0.029970389145638876 2216 64-65 0.02343334390294416 2216 66-67 0.003284829802922218 2216 68-69 -0.03990563799197133 2216 70-71 -0.03449555972147422 2216 72-73 -0.07430676805982728 2216 74-75 -0.19625384859390493 2216 76-77 -0.1701718938769119 2216 78-79 -0.1464815243957105 2216 80-81 -0.21332678728415644 2216 82-83 -0.2630893069045861 2216 84-85 -0.20075497362332229 2216 86-87 -0.2564622436614705 2216 88-89 -0.23417118854005636 2216 90-91 -0.15917386944382272 2216 92-93 -0.2515600103244573 2216 94-95 -0.2628751743462736 2216 96-97 -0.31686754394256056 2216 98-99 -0.3397995382208663 2216 100 -0.3850251660702675 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1070.0 3 238.0 4 231.0 5 495.0 6 1158.0 7 1927.0 8 2376.0 9 3331.0 10 3154.0 11 4668.0 12 4116.0 13 6233.0 14 6116.0 15 6838.0 16 8143.0 17 9578.0 18 13994.0 19 25557.0 20 51788.0 21 61983.0 22 27486.0 23 16312.0 24 15333.0 25 15647.0 26 16385.0 27 18171.0 28 20818.0 29 24437.0 30 28611.0 31 34035.0 32 42766.0 33 58810.0 34 85212.0 35 150006.0 36 235921.0 37 398885.0 38 482610.0 39 139437.0 40 656.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.993781348596142 43.8732706802432 24.233599314677214 18.899348656483436 2 14.46199078549528 43.42198272391508 23.95578578919264 18.160240701396997 3 17.700833405780493 23.86142702463467 18.090022840613614 40.34771672897122 4 36.43434630818382 25.246723687252164 20.33511942513134 17.983810579432678 5 16.740545720382517 42.29812099897258 20.834634078874576 20.126699201770332 6 40.23765745275553 21.93766802453047 22.29429252595272 15.530381996761284 7 18.364469893092956 21.400179503860027 42.70947097732788 17.52587962571914 8 39.336251538627195 21.895480041807193 21.812646083144156 16.955622336421456 9 38.593265011370534 23.614395820861315 21.932476246362125 15.859862921406034 10-11 15.95563814254356 45.41639746864954 22.059468558659486 16.568495830147413 12-13 15.83672177075986 23.16518089118868 32.84030580894745 28.15779152910401 14-15 16.186703890084228 33.88148470856475 21.952159807797557 27.979651593553474 16-17 27.644067863585263 22.57637814566527 32.986240770706516 16.79331322004295 18-19 16.48845263991876 22.895982874066696 33.176482268494645 27.4390822175199 20-21 27.66634461692875 34.21180796351947 22.02679434061798 16.095053078933798 22-23 27.122292954618647 23.148362189385 33.289496041554294 16.439848814442055 24-25 16.144373119318438 34.11899639027686 22.03808089968447 27.69854959072023 26-27 16.664378070772933 27.898905968839205 39.06702342419059 16.369692536197274 28-29 26.99858535207149 22.809370264337634 33.90652753327682 16.285516850314046 30-31 23.59614468924176 24.606699227278206 34.56542055151511 17.23173553196492 32-33 18.743591111427232 26.451397162405925 35.741741795140804 19.063269931026035 34-35 18.838452541130494 26.094598652923246 36.15228111978472 18.914667686161543 36-37 18.978435510033925 26.069308857553253 36.44469437875025 18.507561253662573 38-39 19.642539609154117 25.928091035360275 35.91639944441481 18.512969911070805 40-41 18.381803794654765 27.492748941483757 35.66332861125435 18.46211865260712 42-43 18.445737537826933 26.310306683101864 36.30815061275124 18.93580516631996 44-45 18.448955116540514 27.011798282269677 35.50526739019191 19.033979210997902 46-47 19.173394147388137 26.2166515520624 35.993059136630094 18.616895163919363 48-49 18.53075179844033 26.58278555241409 35.85035949048965 19.036103158655926 50-51 19.087398964303848 27.230540193980634 34.97637972627748 18.705681115438036 52-53 19.287904035034295 26.994579730954904 34.72079082039021 18.99672541362059 54-55 19.719792030948387 28.095406740915923 32.33179322431061 19.85300800382508 56-57 20.378215804936648 29.002332390893304 30.66871751100995 19.9507342931601 58-59 21.422852293764684 29.329148662505705 29.293807161358774 19.95419188237084 60-61 20.790533318317518 29.929731908411426 29.079313144963876 20.200421628307183 62-63 19.92820555882106 30.06971988560308 28.912932878248288 21.08914167732758 64-65 20.31822663213029 29.855072678525207 29.71002681113462 20.11667387820988 66-67 20.3687321316729 29.21280078556427 30.34360533693713 20.0748617458257 68-69 20.6919920258114 29.425961160406455 29.88100459760577 20.001042216176383 70-71 19.198807428087083 30.473413114734665 30.104241375290684 20.22353808188757 72-73 19.41742297688047 29.614546050870278 30.27564037338196 20.692390598867288 74-75 19.558256547775315 30.14926722263288 29.455894189422843 20.836582040168956 76-77 20.264502137666675 29.51995299627739 29.68352259213972 20.53202227391622 78-79 19.929158639057537 29.91130999467282 29.19919180982587 20.960339556443774 80-81 20.314219553590306 30.537715830216527 28.75182513479818 20.396239481394986 82-83 20.101713676888664 30.41363413750462 28.87209796560298 20.61255422000374 84-85 19.923625624289638 30.734610652205163 28.223720314289793 21.118043409215407 86-87 19.903301060838288 30.94808130870804 28.487881884742688 20.66073574571098 88-89 21.04703704362292 30.305818826276887 28.072438469730287 20.5747056603699 90-91 20.3939533590282 31.063472047078605 27.837684715803036 20.704889878090164 92-93 19.77741028543881 31.198321389832316 27.787014480383615 21.23725384434526 94-95 20.103090492024826 31.37665890190918 27.51915010481435 21.001100501251646 96-97 20.145675007711755 30.588590355229783 27.932066407294815 21.33366822976365 98-99 20.897860152574925 30.682062628873553 27.08309922359125 21.33697799496027 100 20.009562703874277 31.63486672475417 27.02506060659945 21.330509964772105 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 25703.0 1 14148.5 2 1917.5 3 1092.5 4 936.0 5 893.5 6 885.5 7 987.0 8 999.5 9 944.5 10 940.0 11 1528.0 12 11105.5 13 13069.5 14 5721.5 15 5717.0 16 6583.5 17 7706.0 18 8913.0 19 10134.5 20 11675.0 21 13406.5 22 15108.5 23 17217.0 24 19558.0 25 21909.0 26 24702.0 27 27607.5 28 30265.5 29 33027.0 30 36143.5 31 39208.0 32 42551.5 33 46342.5 34 50331.0 35 53540.5 36 56060.0 37 58583.5 38 60423.0 39 60842.0 40 60695.0 41 60801.0 42 61060.5 43 61093.0 44 61133.5 45 61673.0 46 62337.0 47 63533.0 48 63902.5 49 67164.0 50 81397.0 51 80186.0 52 60711.0 53 51062.0 54 46995.0 55 44542.0 56 40467.0 57 34752.0 58 29838.5 59 24258.5 60 19276.0 61 15220.0 62 11888.5 63 9061.5 64 6806.5 65 5101.5 66 3849.5 67 2885.0 68 2095.5 69 1485.5 70 1028.0 71 800.5 72 611.0 73 381.5 74 272.5 75 219.5 76 153.0 77 95.5 78 56.5 79 36.0 80 11.0 81 2.5 82 4.5 83 4.0 84 3.0 85 3.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13390749071884267 2 0.005680325131931726 3 0.00335880094757702 4 0.0 5 0.00256849484226478 6 0.013533992053472112 7 0.0031118302896669453 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.9394131582015E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 7.656090395212326E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 7.409119737302251E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 9.878826316403E-5 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.9394131582015E-4 74-75 4.198501184471275E-4 76-77 5.680325131931725E-4 78-79 4.198501184471275E-4 80-81 0.0011854591579683602 82-83 0.0012595503553413825 84-85 0.0015312180790424653 86-87 4.9394131582015E-5 88-89 0.0 90-91 1.4818239474604503E-4 92-93 0.0 94-95 0.0 96-97 0.0012595503553413825 98-99 9.137914342672776E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2024532.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.36506377150963 #Duplication Level Percentage of deduplicated Percentage of total 1 92.92415657816734 65.3861420353649 2 4.811190944652073 6.770795152747054 3 0.8393233579728565 1.7717712482603298 4 0.31945194559479356 0.899130260948419 5 0.1828350167054025 0.6432598805070338 6 0.13629189782812862 0.5754112849329808 7 0.1008830902761827 0.496905155652539 8 0.0802727914557318 0.4518720071919729 9 0.07457053545078453 0.47224444342230665 >10 0.49627614401111053 5.886714253540159 >50 0.019653067239751007 0.944434895698381 >100 0.011233869124941015 1.7578592833677935 >500 0.002597239568582055 1.2518712559311764 >1k 9.125436322045053E-4 1.4085465616646022 >5k 7.019566401573118E-5 0.27521904273223563 >10k+ 2.807826560629247E-4 11.007823238038185 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 169222 8.358573734571744 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25005 1.2351002602082852 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 14896 0.7357749840456955 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 13738 0.6785765796737221 No Hit AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 5572 0.27522410117498763 No Hit AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4626 0.22849725269840143 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 4202 0.20755414090762705 No Hit ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3951 0.1951562138805413 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC 2844 0.14047691021925068 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2302 0.11370529090179854 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0014324298158784352 4.939413158201501E-5 0.0 0.0 0.0 2 0.0014324298158784352 4.939413158201501E-5 0.0 0.0 0.0 3 0.0014324298158784352 4.939413158201501E-5 0.0 4.939413158201501E-5 0.0 4 0.0014324298158784352 4.939413158201501E-5 0.0 4.939413158201501E-5 4.939413158201501E-5 5 0.0014324298158784352 4.939413158201501E-5 0.0 4.939413158201501E-5 4.939413158201501E-5 6 0.0014324298158784352 4.939413158201501E-5 0.0 4.939413158201501E-5 4.939413158201501E-5 7 0.0014324298158784352 9.878826316403001E-5 0.0 4.939413158201501E-5 4.939413158201501E-5 8 0.0014324298158784352 9.878826316403001E-5 0.0 4.939413158201501E-5 9.878826316403001E-5 9 0.00148182394746045 9.878826316403001E-5 0.0 4.939413158201501E-5 9.878826316403001E-5 10-11 0.0015065210132514575 1.48182394746045E-4 0.0 4.939413158201501E-5 9.878826316403001E-5 12-13 0.0015559151448334726 1.48182394746045E-4 0.0 4.939413158201501E-5 1.48182394746045E-4 14-15 0.0015806122106244802 1.48182394746045E-4 0.0 4.939413158201501E-5 1.7287946053705253E-4 16-17 0.0015806122106244802 1.48182394746045E-4 0.0 4.939413158201501E-5 1.9757652632806003E-4 18-19 0.0015806122106244802 1.9757652632806003E-4 0.0 4.939413158201501E-5 1.9757652632806003E-4 20-21 0.0016053092764154878 2.716677237010825E-4 0.0 4.939413158201501E-5 2.46970657910075E-4 22-23 0.0017040975395795177 2.9636478949209E-4 0.0 4.939413158201501E-5 2.46970657910075E-4 24-25 0.0017781887369525402 2.9636478949209E-4 0.0 4.939413158201501E-5 2.9636478949209E-4 26-27 0.0018275828685345552 2.9636478949209E-4 0.0 4.939413158201501E-5 3.4575892107410505E-4 28-29 0.001926371131698585 2.9636478949209E-4 0.0 4.939413158201501E-5 3.4575892107410505E-4 30-31 0.0020992505922356377 2.9636478949209E-4 0.0 4.939413158201501E-5 3.4575892107410505E-4 32-33 0.00217334178960866 2.9636478949209E-4 0.0 4.939413158201501E-5 3.4575892107410505E-4 34-35 0.0024450095133097425 2.9636478949209E-4 0.0 7.409119737302251E-5 3.4575892107410505E-4 36-37 0.0030130420265029154 2.9636478949209E-4 0.0 9.878826316403001E-5 3.704559868651125E-4 38-39 0.00414910705288926 2.9636478949209E-4 0.0 1.48182394746045E-4 3.9515305265612005E-4 40-41 0.006297751776706913 2.9636478949209E-4 0.0 1.48182394746045E-4 3.9515305265612005E-4 42-43 0.008915640750553708 2.9636478949209E-4 0.0 1.48182394746045E-4 3.9515305265612005E-4 44-45 0.011508832658609497 2.9636478949209E-4 0.0 1.48182394746045E-4 3.9515305265612005E-4 46-47 0.014917027737768531 3.210618552830975E-4 0.0 1.48182394746045E-4 3.9515305265612005E-4 48-49 0.018028858027435476 3.704559868651125E-4 0.0 1.9757652632806003E-4 4.4454718423813505E-4 50-51 0.022153268014533732 3.9515305265612005E-4 0.0 1.9757652632806003E-4 4.9394131582015E-4 52-53 0.028154655001748553 3.9515305265612005E-4 0.0 1.9757652632806003E-4 4.9394131582015E-4 54-55 0.03610711018645297 4.4454718423813505E-4 0.0 2.9636478949209E-4 5.43335447402165E-4 56-57 0.0447757802790966 4.692442500291425E-4 0.0 2.9636478949209E-4 5.43335447402165E-4 58-59 0.05475339485866364 5.43335447402165E-4 0.0 2.9636478949209E-4 5.43335447402165E-4 60-61 0.06725011014891344 5.43335447402165E-4 0.0 2.9636478949209E-4 5.43335447402165E-4 62-63 0.0830068381235762 5.43335447402165E-4 0.0 3.4575892107410505E-4 5.43335447402165E-4 64-65 0.10227054944056208 5.680325131931725E-4 0.0 3.4575892107410505E-4 6.42123710566195E-4 66-67 0.12402866440243968 5.9272957898418E-4 0.0 3.9515305265612005E-4 6.915178421482101E-4 68-69 0.15168937808836808 6.668207763572025E-4 0.0 3.9515305265612005E-4 6.915178421482101E-4 70-71 0.18759891174849297 6.915178421482101E-4 0.0 3.9515305265612005E-4 6.915178421482101E-4 72-73 0.22780573485625322 6.915178421482101E-4 0.0 3.9515305265612005E-4 6.915178421482101E-4 74-75 0.2756439512934347 6.915178421482101E-4 0.0 4.9394131582015E-4 7.656090395212326E-4 76-77 0.3341019060207495 7.40911973730225E-4 0.0 4.9394131582015E-4 8.150031711032476E-4 78-79 0.401598986827573 7.656090395212326E-4 0.0 5.186383816111575E-4 8.39700236894255E-4 80-81 0.48722371392499597 8.39700236894255E-4 0.0 5.43335447402165E-4 8.39700236894255E-4 82-83 0.589173201510275 8.39700236894255E-4 0.0 5.43335447402165E-4 8.643973026852625E-4 84-85 0.7119176184915823 8.39700236894255E-4 0.0 6.42123710566195E-4 9.137914342672776E-4 86-87 0.8519746785923858 8.39700236894255E-4 0.0 6.668207763572025E-4 9.38488500058285E-4 88 0.9755834928763784 8.39700236894255E-4 0.0 6.915178421482101E-4 9.38488500058285E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 3465 0.0 89.39373 1 AACGTGT 1615 0.0 75.20238 1 ATCGAGT 1090 0.0 74.714355 1 CGTGTGG 1830 0.0 73.97627 3 GTGGAAT 59305 0.0 71.090126 6 TGGAATC 59555 0.0 70.750496 7 AACGAGT 50260 0.0 70.66774 1 ACGAGTG 54860 0.0 70.53274 2 ACGTGTG 1775 0.0 70.44939 2 TCGAGTG 1210 0.0 70.32134 2 GAATCAC 59985 0.0 70.19457 9 GGAATCA 60065 0.0 70.12455 8 CGAGTGG 56880 0.0 70.120224 3 AGTGGAA 58520 0.0 69.70367 5 GAGTGGA 59240 0.0 68.93584 4 AGCGAGT 680 0.0 60.22749 1 GCGAGTG 725 0.0 58.357735 2 ATGAGTG 2030 0.0 52.56828 2 GTGTGGA 2755 0.0 51.525906 4 TGAGTGG 2300 0.0 50.275723 3 >>END_MODULE