FastQCFastQC Report
Thu 26 May 2016
SRR2132789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132789_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871768
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1377517.359405652837317No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA325471.7388372918011208No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG141920.7582136247654624No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111880.597723649512119No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44480.23763628825794653No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38470.20552760812237414No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC36950.1974069435955738No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36430.1946288215206158No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31870.1702668279402148No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27150.14505002756751906No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA21960.1173222322424574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT27900.088.094311
AACGTGT12950.073.0815661
ATCGAGT9100.072.438291
CGTGTGG14450.069.305263
GTGGAAT499250.068.4166956
TGGAATC504050.067.696557
ACGAGTG464200.067.2455442
AACGAGT426450.067.218391
GAATCAC508600.067.115079
GGAATCA508650.067.108478
CGAGTGG482200.066.800723
ACGTGTG14850.066.173762
TCGAGTG10800.066.173762
GAGTGGA500650.066.1086044
AGTGGAA500950.066.0971765
GCGAGTG5900.056.581222
AGCGAGT6150.052.826951
ATGAGTG18850.049.886682
TGAGTGG20400.049.3216933
AATGAGT17700.049.213021