##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132789_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1871768
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.884908279231187	31.0	25.0	33.0	16.0	34.0
2	28.174666945903553	31.0	25.0	34.0	16.0	34.0
3	29.25009883703536	31.0	28.0	34.0	16.0	34.0
4	34.75235926674674	35.0	35.0	37.0	32.0	37.0
5	33.853394224070506	35.0	35.0	37.0	28.0	37.0
6	35.25610385475123	37.0	35.0	37.0	32.0	37.0
7	35.64855954370413	37.0	35.0	37.0	33.0	37.0
8	35.92415726735365	37.0	35.0	37.0	35.0	37.0
9	37.88727823106282	39.0	38.0	39.0	35.0	39.0
10-11	37.88823080638198	39.0	38.0	39.0	35.0	39.0
12-13	37.87052054528125	39.0	38.0	39.0	35.0	39.0
14-15	39.295531016664455	41.0	39.0	41.0	36.0	41.0
16-17	39.284976824050844	40.5	39.0	41.0	36.0	41.0
18-19	39.19323762346616	40.5	39.0	41.0	36.0	41.0
20-21	39.13453296562395	40.5	39.0	41.0	35.5	41.0
22-23	39.011900246184354	40.0	39.0	41.0	35.0	41.0
24-25	38.895018506567055	40.0	39.0	41.0	35.0	41.0
26-27	38.734911324480386	40.0	39.0	41.0	35.0	41.0
28-29	38.557856796355104	40.0	38.5	41.0	35.0	41.0
30-31	38.41217581452402	40.0	38.0	41.0	34.5	41.0
32-33	38.25675003526078	40.0	38.0	41.0	34.0	41.0
34-35	38.15230840574259	40.0	38.0	41.0	34.0	41.0
36-37	37.962983393241046	40.0	38.0	41.0	34.0	41.0
38-39	37.464599245205605	40.0	36.0	41.0	33.0	41.0
40-41	37.20497786050408	40.0	35.0	41.0	33.0	41.0
42-43	37.03395319291707	40.0	35.0	41.0	33.0	41.0
44-45	36.87803349560416	40.0	35.0	41.0	33.0	41.0
46-47	36.72508478614871	39.5	35.0	41.0	32.5	41.0
48-49	36.53877002919165	39.0	35.0	41.0	31.5	41.0
50-51	36.22293361143048	39.0	35.0	41.0	31.0	41.0
52-53	35.7269634912019	39.0	35.0	41.0	29.5	41.0
54-55	34.8059484401913	39.0	35.0	41.0	24.5	41.0
56-57	34.05257783015844	39.0	35.0	41.0	15.0	41.0
58-59	33.67192034482906	38.0	34.0	40.0	7.5	41.0
60-61	33.40157941582504	37.5	34.0	40.0	3.5	41.0
62-63	33.112961916220385	37.0	34.0	40.0	2.0	41.0
64-65	32.81394382209761	36.5	34.0	40.0	2.0	41.0
66-67	32.47306450372055	36.0	33.5	39.5	2.0	41.0
68-69	32.152610526518245	35.5	33.0	39.0	2.0	41.0
70-71	31.818649800616313	35.0	33.0	39.0	2.0	40.5
72-73	31.489499232810903	35.0	33.0	38.5	2.0	40.0
74-75	31.150358110620544	35.0	33.0	37.0	2.0	40.0
76-77	30.81420774369473	35.0	33.0	37.0	2.0	39.0
78-79	30.475311844202913	35.0	32.5	36.0	2.0	39.0
80-81	30.15572763291177	35.0	32.0	36.0	2.0	38.5
82-83	29.844113960704533	35.0	32.0	35.5	2.0	37.0
84-85	29.554703093545783	35.0	32.0	35.0	2.0	37.0
86-87	29.35254956810887	35.0	32.0	35.0	2.0	36.5
88-89	29.122887825841666	35.0	31.0	35.0	2.0	36.0
90-91	28.91649339020648	35.0	31.0	35.0	2.0	36.0
92-93	28.738027362365422	35.0	31.0	35.0	2.0	36.0
94-95	28.53256386475247	35.0	31.0	35.0	2.0	35.0
96-97	28.321116185339207	34.5	30.5	35.0	2.0	35.0
98-99	28.150804480042396	34.0	30.0	35.0	2.0	35.0
100	28.053115022801972	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.17450955019218384
1101	2	2.4309476936370267
1101	3	1.145344814124062
1101	4	0.6755001539690255
1101	5	2.250162363892599
1101	6	0.9990116073404423
1101	7	0.5236583912159958
1101	8	0.3736198988824668
1101	9	0.3193678035103318
1101	10-11	0.4823133966016968
1101	12-13	0.3997715785015643
1101	14-15	0.5193198279420628
1101	16-17	0.42828870251742046
1101	18-19	0.4785574547763787
1101	20-21	0.4430679085200069
1101	22-23	0.40898751609987016
1101	24-25	0.3985424387074872
1101	26-27	0.4409059698179476
1101	28-29	0.382521561800246
1101	30-31	0.4308269751554832
1101	32-33	0.3842975634765722
1101	34-35	0.40716059299376894
1101	36-37	0.42284882702259097
1101	38-39	0.29613498635967517
1101	40-41	0.3605836733970804
1101	42-43	0.43607810106582434
1101	44-45	0.4740859420527954
1101	46-47	0.45469811279377836
1101	48-49	0.42517006003955515
1101	50-51	0.373167500181502
1101	52-53	0.4705204192157453
1101	54-55	0.5485436951630689
1101	56-57	0.4523682372388862
1101	58-59	0.39433566658819075
1101	60-61	0.2763285704089853
1101	62-63	0.3277898761910265
1101	64-65	0.3548164786675514
1101	66-67	0.348367719294572
1101	68-69	0.2958046260804821
1101	70-71	0.250113940292227
1101	72-73	0.2773973785695354
1101	74-75	0.2639735351555039
1101	76-77	0.3707884273429016
1101	78-79	0.39277940302307
1101	80-81	0.44258476941885405
1101	82-83	0.45533686499449644
1101	84-85	0.36166098475888475
1101	86-87	0.38854072752939217
1101	88-89	0.4522024388898238
1101	90-91	0.3370598676012797
1101	92-93	0.3711770985888201
1101	94-95	0.42358667504408487
1101	96-97	0.3778408350974125
1101	98-99	0.4056613776454583
1101	100	0.5008474010873059
1102	1	1.4688959345936503
1102	2	2.9912978027113013
1102	3	3.2438685806664296
1102	4	1.45867831173409
1102	5	2.6859722281524157
1102	6	1.266227422196053
1102	7	0.7381773653778723
1102	8	0.5285459702911481
1102	9	0.39212295886942883
1102	10-11	0.48069949699765147
1102	12-13	0.45946800721724657
1102	14-15	0.6041637120661179
1102	16-17	0.5515953515201915
1102	18-19	0.4427098364899891
1102	20-21	0.32573143383624625
1102	22-23	0.3170112883818703
1102	24-25	0.43639382644430924
1102	26-27	0.3403321978973395
1102	28-29	0.4712925236818535
1102	30-31	0.3926805277966281
1102	32-33	0.3504370271908286
1102	34-35	0.3911434047078828
1102	36-37	0.3823348875812371
1102	38-39	0.475489039624307
1102	40-41	0.4812418009079025
1102	42-43	0.5540956787963935
1102	44-45	0.554388714040158
1102	46-47	0.5968191705697734
1102	48-49	0.545398565435022
1102	50-51	0.7147259039174543
1102	52-53	0.8174824742932572
1102	54-55	0.8603469941337138
1102	56-57	0.9779607267385515
1102	58-59	1.0376417458792986
1102	60-61	1.0446357657397272
1102	62-63	0.9576912565513638
1102	64-65	1.0322401022293022
1102	66-67	1.0015338493410297
1102	68-69	0.9781805958126313
1102	70-71	0.9908363559377058
1102	72-73	0.8774090916712467
1102	74-75	0.9597671711361144
1102	76-77	0.9274360483502981
1102	78-79	0.9727103743772041
1102	80-81	1.0318466824510963
1102	82-83	1.005222561277808
1102	84-85	0.9653137506027143
1102	86-87	0.9386762041123298
1102	88-89	0.9015581545220748
1102	90-91	0.882312723918723
1102	92-93	0.8943767991075582
1102	94-95	0.9343409392721007
1102	96-97	0.8659509739667577
1102	98-99	0.9433047568519157
1102	100	0.9047872123256759
1103	1	1.2671978106612016
1103	2	2.9761306691475617
1103	3	3.235006875592454
1103	4	1.4269760072956998
1103	5	2.6262506571045776
1103	6	1.2204996614193604
1103	7	0.7139606660102729
1103	8	0.49851768233531857
1103	9	0.39886397430908005
1103	10-11	0.4545958252879032
1103	12-13	0.4408628408859201
1103	14-15	0.5327861083708214
1103	16-17	0.44975891210184926
1103	18-19	0.49701872064967034
1103	20-21	0.43311812982960873
1103	22-23	0.4145782298922782
1103	24-25	0.4789390410147263
1103	26-27	0.5522487764454951
1103	28-29	0.6401885919502774
1103	30-31	0.6436651840901035
1103	32-33	0.6047040055074291
1103	34-35	0.6179168039510401
1103	36-37	0.5971601831494553
1103	38-39	0.6104731218540991
1103	40-41	0.6516243272799613
1103	42-43	0.611728004709768
1103	44-45	0.6690066371631929
1103	46-47	0.6686876914406525
1103	48-49	0.6656574504211221
1103	50-51	0.7830548020981496
1103	52-53	0.8656087852366312
1103	54-55	0.9031336863376183
1103	56-57	0.7359445054079643
1103	58-59	0.8194440277018415
1103	60-61	0.8305587887978163
1103	62-63	0.7173556026408221
1103	64-65	0.663018885738353
1103	66-67	0.752983319843878
1103	68-69	0.6703544629338154
1103	70-71	0.6964604063037321
1103	72-73	0.6576439861164971
1103	74-75	0.7333288775404299
1103	76-77	0.7193439070967642
1103	78-79	0.7759667748446759
1103	80-81	0.7808485211480694
1103	82-83	0.7184928658581029
1103	84-85	0.6952121485860765
1103	86-87	0.7046561578405424
1103	88-89	0.6624717139951137
1103	90-91	0.6914715190461145
1103	92-93	0.7484768393807038
1103	94-95	0.7639584129000454
1103	96-97	0.7590476363749765
1103	98-99	0.7066094085012828
1103	100	0.8064796117466138
1104	1	6.085185474550798
1104	2	5.153984076141228
1104	3	4.1976054876561335
1104	4	1.879935579637774
1104	5	2.858176051810169
1104	6	1.3225100837132615
1104	7	0.7620098503317649
1104	8	0.5513231962617624
1104	9	0.46814108159450285
1104	10-11	0.4963720185543963
1104	12-13	0.40352435727668734
1104	14-15	0.5215533017436798
1104	16-17	0.39484982029165394
1104	18-19	0.3583098186199152
1104	20-21	0.3498670168995659
1104	22-23	0.37237724179873055
1104	24-25	0.3317399610191032
1104	26-27	0.4005221098525311
1104	28-29	0.388787350409487
1104	30-31	0.46348936840206534
1104	32-33	0.4625717737495876
1104	34-35	0.44775573837461735
1104	36-37	0.42241710920180253
1104	38-39	0.4657456100557198
1104	40-41	0.41232578296234124
1104	42-43	0.46809226422480066
1104	44-45	0.5408520328142572
1104	46-47	0.5220457912877379
1104	48-49	0.6594708098514914
1104	50-51	0.6813379333306813
1104	52-53	0.39816501503337776
1104	54-55	0.4698000734294965
1104	56-57	0.5938308590194978
1104	58-59	0.5959501002568359
1104	60-61	0.5551386736714576
1104	62-63	0.6115415653686753
1104	64-65	0.5657175194944912
1104	66-67	0.6037330822370777
1104	68-69	0.586245277796472
1104	70-71	0.5230202144035907
1104	72-73	0.41688425052816314
1104	74-75	0.433942499720267
1104	76-77	0.39260701378517027
1104	78-79	0.3607965004560043
1104	80-81	0.35026068211757533
1104	82-83	0.33988687530747796
1104	84-85	0.31412277144947254
1104	86-87	0.315606047491098
1104	88-89	0.34559688483754414
1104	90-91	0.34981248032335444
1104	92-93	0.480984099111911
1104	94-95	0.38632877375357566
1104	96-97	0.34735492286911196
1104	98-99	0.34755617962217045
1104	100	0.363074634832504
1105	1	1.651262293525253
1105	2	3.1686916089529262
1105	3	3.2473138832802775
1105	4	1.3949835533213388
1105	5	2.54871061517742
1105	6	1.1763645507677865
1105	7	0.7288606148810217
1105	8	0.47187634429058534
1105	9	0.354612856947746
1105	10-11	0.41470148062971646
1105	12-13	0.3247064403064073
1105	14-15	0.46279272948074635
1105	16-17	0.32974040986516684
1105	18-19	0.32339467495929597
1105	20-21	0.31184120484878974
1105	22-23	0.27599659480369354
1105	24-25	0.266480940225982
1105	26-27	0.2582412927869484
1105	28-29	0.3053412550152146
1105	30-31	0.356688364054655
1105	32-33	0.38984933161376034
1105	34-35	0.3238547000580567
1105	36-37	0.29448764547767325
1105	38-39	0.30938947405400086
1105	40-41	0.26296723389622656
1105	42-43	0.32126196172706756
1105	44-45	0.42039587389788124
1105	46-47	0.41080462855757105
1105	48-49	0.362937254878716
1105	50-51	0.2419752866080671
1105	52-53	0.26698871593855245
1105	54-55	0.15624939317542186
1105	56-57	0.20050970186527906
1105	58-59	0.17836555166325496
1105	60-61	0.14134689307951476
1105	62-63	0.12506907803122402
1105	64-65	0.07865881620885062
1105	66-67	-0.0216708517005344
1105	68-69	0.08908127017224388
1105	70-71	0.1724521165799615
1105	72-73	0.2064311325338295
1105	74-75	0.2356266046914719
1105	76-77	0.09773626983474415
1105	78-79	0.07987380826383017
1105	80-81	0.12914822364920653
1105	82-83	0.06253782860749624
1105	84-85	0.12056129151908834
1105	86-87	0.10577347994675179
1105	88-89	0.06550716733227091
1105	90-91	0.12316200255384402
1105	92-93	0.14558491860573852
1105	94-95	0.21051677918039502
1105	96-97	0.12936713826982071
1105	98-99	0.19069163068384398
1105	100	0.3086792284047455
1106	1	-0.7312016967358232
1106	2	1.8640436415838266
1106	3	2.6504623718171665
1106	4	1.1525189662375368
1106	5	2.4241808760941552
1106	6	1.0582146935557049
1106	7	0.5547684788429379
1106	8	0.39584619862184667
1106	9	0.24554168717712344
1106	10-11	0.37210282841610365
1106	12-13	0.3079233836444075
1106	14-15	0.32691934159552005
1106	16-17	0.27604095065924383
1106	18-19	0.22638104578553708
1106	20-21	0.2674141135351036
1106	22-23	0.2598299201985199
1106	24-25	0.31669066028742776
1106	26-27	0.22087674715554328
1106	28-29	0.30956650164934274
1106	30-31	0.25802586864320887
1106	32-33	0.28089986968196
1106	34-35	0.32263633046678564
1106	36-37	0.3576057132717665
1106	38-39	0.3970875039460111
1106	40-41	0.4445069810461604
1106	42-43	0.45924780555119327
1106	44-45	0.5361604965806279
1106	46-47	0.5475662684657294
1106	48-49	0.6024191151981029
1106	50-51	0.6171142343981231
1106	52-53	0.6018440428587226
1106	54-55	0.48576884758763583
1106	56-57	0.6257482348826855
1106	58-59	0.6705441133702337
1106	60-61	0.5701480943000163
1106	62-63	0.6077499808327076
1106	64-65	0.6864020554220929
1106	66-67	0.642187448429759
1106	68-69	0.585074810714012
1106	70-71	0.6425677490695314
1106	72-73	0.6477519337377231
1106	74-75	0.6177577036888664
1106	76-77	0.6076211939971294
1106	78-79	0.5975976736933148
1106	80-81	0.6422716284157133
1106	82-83	0.6325325566734463
1106	84-85	0.5512669693499781
1106	86-87	0.5187205255116787
1106	88-89	0.5222245601045579
1106	90-91	0.5197244949620519
1106	92-93	0.5544299556900434
1106	94-95	0.5188268373839975
1106	96-97	0.629399561811951
1106	98-99	0.6367517207849467
1106	100	0.6828689985189627
1107	1	-0.8880825519406343
1107	2	1.8496998497930228
1107	3	-0.7528636820048966
1107	4	-0.15536762352905953
1107	5	1.8537031876165102
1107	6	0.7489432795935045
1107	7	0.28548945393806235
1107	8	0.2539524315686066
1107	9	0.24030407282830168
1107	10-11	0.26754860164754746
1107	12-13	0.2526001961049502
1107	14-15	0.3134871858019963
1107	16-17	0.22613237381367668
1107	18-19	0.18593313821575919
1107	20-21	0.08878087671618573
1107	22-23	0.1872007228494894
1107	24-25	0.114116696180254
1107	26-27	0.23683516888722522
1107	28-29	0.18270294876067794
1107	30-31	0.24631755542536382
1107	32-33	0.18427893256168204
1107	34-35	0.20637448928152935
1107	36-37	0.2718503351983941
1107	38-39	0.2983051749097143
1107	40-41	0.2687023914001614
1107	42-43	0.35344891412930934
1107	44-45	0.38813125554653993
1107	46-47	0.42214204626134233
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2216	1	2.876490589905135
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2216	4	-0.9239026975986206
2216	5	-2.2477941591698603
2216	6	-1.0918778842675252
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2216	58-59	0.2153300036967849
2216	60-61	0.22971846361047454
2216	62-63	0.21334988356344553
2216	64-65	0.2626475946410025
2216	66-67	0.28485200472864847
2216	68-69	0.271680797465379
2216	70-71	0.2432366512091484
2216	72-73	0.20744781871603735
2216	74-75	0.20008814318331503
2216	76-77	0.30370751343440716
2216	78-79	0.2743638458818083
2216	80-81	0.1968918599541034
2216	82-83	0.07868631561455786
2216	84-85	0.22817711021839315
2216	86-87	0.3019739674675712
2216	88-89	0.27708303879445495
2216	90-91	0.26325479856101097
2216	92-93	0.24374280774380352
2216	94-95	0.2133031119710722
2216	96-97	0.10749198559944872
2216	98-99	-0.004960179218496563
2216	100	-0.05153371032065479
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	857.0
3	193.0
4	235.0
5	533.0
6	1444.0
7	2396.0
8	2926.0
9	4093.0
10	3314.0
11	4065.0
12	3933.0
13	5568.0
14	5837.0
15	6398.0
16	7597.0
17	9012.0
18	12618.0
19	21993.0
20	42090.0
21	49481.0
22	22199.0
23	14135.0
24	13790.0
25	14563.0
26	15738.0
27	17005.0
28	20275.0
29	23120.0
30	26960.0
31	32763.0
32	41905.0
33	57293.0
34	84166.0
35	156068.0
36	239522.0
37	389538.0
38	406260.0
39	111376.0
40	509.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.923331451973153	41.69729003886658	24.235628325259533	20.14375018390073
2	15.260464835470728	41.06988774214299	24.384815082549142	19.28483233983714
3	18.881607945696572	24.448510849733264	18.162317245506102	38.507563959064065
4	34.12586388911446	26.041528650986663	20.566865124310276	19.265742335588598
5	17.667552658643764	39.75434425463143	21.095566923559815	21.482536163164994
6	38.40309688174571	22.502992156276047	22.647630853405715	16.446280108572527
7	19.50326759672428	21.952362329247237	40.007116479458375	18.537253594570107
8	37.25894448457288	22.399731163263823	22.29645981766971	18.04486453449359
9	36.5900047441777	24.11661060558787	22.45946078787542	16.83392386235901
10-11	16.978626624667157	43.0019639186053	22.341043334430335	17.678366122297206
12-13	16.840922592970927	23.900932166807	31.615536754555052	27.642608485667026
14-15	17.235362502190444	33.0741844074693	22.213944249501004	27.476508840839248
16-17	27.0838586833411	23.312744955571418	31.80070927593591	17.80268708515158
18-19	17.48135986938552	23.628141949215927	31.972311739489083	26.91818644190947
20-21	27.142119108778438	33.429463480516816	22.324374601980587	17.104042808724156
22-23	26.585533036145502	23.883034649593327	32.09842245406482	17.43300986019635
24-25	17.144833120343975	33.40104115467302	22.271349868146054	27.18277585683696
26-27	17.71333779435747	27.830887116054747	37.119113351417845	17.33666173816994
28-29	26.381661616183198	23.6223987160802	32.71361621739447	17.282323450342137
30-31	23.688940082317895	25.124534664552446	33.10800270118946	18.0785225519402
32-33	19.54854982027687	26.888481905877228	33.97442952331699	19.58853875052891
34-35	19.50837390103902	26.56344162310714	34.37674968265298	19.551434793200865
36-37	19.79801984006565	26.3979029452368	34.68196913292673	19.12210808177082
38-39	20.49468737578589	26.13852250919986	34.09343465643178	19.273355458582476
40-41	19.055753704518935	27.749619610977426	33.94584692119963	19.248779763304
42-43	19.127073594229998	26.59545879524509	34.621931347669296	19.655536262855616
44-45	19.259544986344462	27.231953960106164	33.608598928927094	19.899902124622283
46-47	19.982791670762616	26.486295310102538	34.098055955652626	19.432857063482224
48-49	19.311581349825406	26.851778639233064	34.01348350863996	19.82315650230157
50-51	19.7623583691996	27.602726406264022	33.141179889815405	19.49373533472097
52-53	19.995079511829875	27.156954223165243	32.97194547073338	19.876020794271504
54-55	20.292525569408173	28.233039564732383	30.847038735569793	20.62739613028965
56-57	20.593150433173342	29.070162541511557	29.591487833962326	20.745199191352775
58-59	21.747593718879692	29.211713203773115	28.28499579007655	20.755697287270642
60-61	21.270504677930173	29.8025182608101	28.03119831090178	20.895778750357948
62-63	20.320301789860483	29.87841428896561	27.797545205971257	22.003738715202648
64-65	20.817216663603606	29.777942565531625	28.619091682302507	20.78574908856226
66-67	20.95225502098952	29.060687291557315	29.15912366252753	20.827934024925636
68-69	21.283353492526853	29.27267161314864	28.77763162956093	20.666343264763583
70-71	19.758057622525868	30.27645520171303	29.123828380440308	20.84165879532079
72-73	19.772086164892936	29.485510962943966	29.39094755737915	21.351455314783944
74-75	20.056786111257292	29.980090935779447	28.494003372760563	21.469119580202705
76-77	20.89317125749463	29.256220423852987	28.83581339997302	21.01479491867937
78-79	20.540592955451807	29.668181195287108	28.166839810349565	21.624386038911517
80-81	21.002392946497693	30.27312393582266	27.725829984864443	20.99865313281521
82-83	20.77766240746296	30.290335170205644	27.70265054022959	21.229351882101806
84-85	20.636545042193248	30.712597068510934	26.984765553000006	21.666092336295808
86-87	20.57770026336084	30.812871156738836	27.297154853449936	21.312273726450385
88-89	21.890132751494843	30.06473024434652	26.988601151424753	21.056535852733884
90-91	21.03879861179377	30.888176312448977	26.856693778288765	21.21633129746849
92-93	20.38623376401349	31.09453201465139	26.74458052493685	21.77465369639827
94-95	20.732804492864503	31.322311312085688	26.495003654299037	21.449880540750776
96-97	20.761946128922908	30.469697686091575	26.940774298228952	21.827581886756565
98-99	21.531780442594965	30.657459984779003	25.995047966976475	21.81571160564956
100	20.658436301934856	31.54947621713802	25.80138136777635	21.990706113150775
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	33898.0
1	18506.0
2	2323.0
3	1344.0
4	1146.5
5	1089.5
6	1079.0
7	1203.0
8	1208.0
9	1140.5
10	1144.0
11	1746.5
12	12106.0
13	13829.0
14	5275.5
15	4999.0
16	5765.5
17	6724.0
18	7606.0
19	8444.0
20	9486.5
21	10821.0
22	12208.5
23	13829.5
24	15720.5
25	17625.0
26	20172.5
27	22912.5
28	25179.0
29	27467.0
30	29690.0
31	31821.0
32	34411.0
33	37206.0
34	39996.5
35	42696.0
36	45231.0
37	46827.5
38	47337.0
39	47576.0
40	47972.0
41	48355.5
42	48432.5
43	49317.0
44	50752.0
45	51990.5
46	53425.5
47	55778.0
48	57412.0
49	62180.5
50	79012.0
51	80454.5
52	62523.5
53	54461.0
54	53405.5
55	53568.5
56	49451.0
57	42493.0
58	37134.5
59	30650.0
60	24662.0
61	20289.5
62	16275.0
63	12456.5
64	9302.0
65	6972.0
66	5194.5
67	4013.5
68	3162.5
69	2386.0
70	1751.5
71	1303.0
72	1006.5
73	772.5
74	552.0
75	380.0
76	272.0
77	172.5
78	109.0
79	61.5
80	30.0
81	15.5
82	7.0
83	2.0
84	1.0
85	3.0
86	3.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1363951087955345
2	0.005075415329250206
3	0.003365801744660663
4	0.0
5	0.0022972932542921986
6	0.011112488299832031
7	0.0029918237730317005
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	2.6712712259211613E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	9.616576413316181E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.3356356129605806E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	1.0685084903684645E-4
64-65	0.0
66-67	2.6712712259211613E-5
68-69	0.0
70-71	0.0
72-73	4.274033961473858E-4
74-75	4.006906838881742E-4
76-77	5.609669574434438E-4
78-79	4.541161084065974E-4
80-81	9.082322168131948E-4
82-83	9.349449290724064E-4
84-85	0.0012287847639237343
86-87	8.013813677763484E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0011486466271460993
98-99	6.143923819618671E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1871768.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.64412784405383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.52075237718883	62.823622265637425
2	6.006766453176375	8.246584886824257
3	1.0652064040717366	2.1936049374421542
4	0.3465071289551794	0.9514271863550149
5	0.1865865052791736	0.6404033961179407
6	0.11272233998529074	0.4642636030098718
7	0.08483368807128905	0.40763341706137046
8	0.07334366144208165	0.4027689338065003
9	0.05957590922650173	0.36805826964327576
>10	0.49316234868375125	6.12666617591823
>50	0.03012587351189293	1.4242535802423835
>100	0.01605374915265304	2.241147986456783
>500	0.002649305047856013	1.2895959239572141
>1k	0.0014025732606296538	1.9535425935582467
>5k	0.0	0.0
>10k+	3.116829468065897E-4	10.466426843969295
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	137751	7.359405652837317	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32547	1.7388372918011208	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	14192	0.7582136247654624	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11188	0.597723649512119	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4448	0.23763628825794653	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3847	0.20552760812237414	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	3695	0.1974069435955738	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3643	0.1946288215206158	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3187	0.1702668279402148	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2715	0.14505002756751906	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	2196	0.1173222322424574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010150830658500413	5.3425424518423226E-5	0.0	0.0	0.0
2	0.0010150830658500413	1.0685084903684645E-4	0.0	0.0	0.0
3	0.0010150830658500413	1.0685084903684645E-4	0.0	0.0	0.0
4	0.0010685084903684645	1.0685084903684645E-4	0.0	0.0	0.0
5	0.0010685084903684645	1.6027627355526966E-4	0.0	0.0	0.0
6	0.0010685084903684645	1.6027627355526966E-4	0.0	0.0	0.0
7	0.0010685084903684645	2.137016980736929E-4	0.0	0.0	0.0
8	0.0011219339148868877	2.137016980736929E-4	0.0	0.0	0.0
9	0.0011219339148868877	2.671271225921161E-4	0.0	0.0	0.0
10-11	0.0011753593394053109	2.938398348513277E-4	0.0	0.0	0.0
12-13	0.001228784763923734	3.205525471105393E-4	0.0	0.0	0.0
14-15	0.0012554974761829457	3.205525471105393E-4	0.0	0.0	0.0
16-17	0.001308922900701369	3.205525471105393E-4	0.0	0.0	0.0
18-19	0.0013623483252197923	3.205525471105393E-4	0.0	0.0	0.0
20-21	0.0013890610374790039	4.0069068388817416E-4	0.0	0.0	0.0
22-23	0.001442486461997427	4.274033961473858E-4	0.0	0.0	0.0
24-25	0.001442486461997427	4.274033961473858E-4	0.0	0.0	0.0
26-27	0.001442486461997427	4.541161084065974E-4	0.0	0.0	0.0
28-29	0.0015226245987750619	4.80828820665809E-4	0.0	0.0	0.0
30-31	0.0016829008723303314	5.342542451842322E-4	0.0	0.0	0.0
32-33	0.0019233152826632362	6.411050942210786E-4	0.0	0.0	0.0
34-35	0.002564420376884315	6.678178064802903E-4	0.0	0.0	0.0
36-37	0.003686354291771203	6.945305187395019E-4	0.0	0.0	0.0
38-39	0.005663094998952862	6.945305187395019E-4	0.0	0.0	0.0
40-41	0.009322736578464852	6.945305187395019E-4	0.0	0.0	0.0
42-43	0.013489919690901863	7.479559432579251E-4	0.0	0.0	0.0
44-45	0.018351633322078377	8.013813677763483E-4	0.0	0.0	0.0
46-47	0.023453761363587795	8.013813677763483E-4	0.0	0.0	0.0
48-49	0.029944950442576217	8.013813677763483E-4	0.0	0.0	0.0
50-51	0.036997106479008085	8.013813677763483E-4	0.0	0.0	0.0
52-53	0.045865726949066335	8.013813677763483E-4	0.0	0.0	0.0
54-55	0.05793987289022999	8.548067922947716E-4	0.0	0.0	0.0
56-57	0.07215103581213056	9.082322168131948E-4	0.0	0.0	0.0
58-59	0.08863277927606414	9.883703535908297E-4	0.0	0.0	0.0
60-61	0.10989609823439657	0.0010685084903684645	0.0	0.0	0.0
62-63	0.13636839608327528	0.0011486466271460993	0.0	0.0	0.0
64-65	0.16572566685614884	0.0011753593394053109	0.0	0.0	0.0
66-67	0.19900970633112652	0.0011753593394053109	0.0	0.0	0.0
68-69	0.24562338922345078	0.0011753593394053109	0.0	0.0	0.0
70-71	0.3028687315949412	0.001228784763923734	0.0	0.0	0.0
72-73	0.36842172747904656	0.0013623483252197923	0.0	0.0	0.0
74-75	0.4401186471827705	0.0013890610374790039	0.0	0.0	0.0
76-77	0.524878083181249	0.0014157737497382155	0.0	0.0	0.0
78-79	0.6244630744835898	0.001442486461997427	0.0	0.0	0.0
80-81	0.7454182355933	0.001442486461997427	0.0	0.0	0.0
82-83	0.889186052972377	0.0014691991742566387	0.0	0.0	0.0
84-85	1.0658372191425434	0.0014959118865158503	0.0	0.0	0.0
86-87	1.2730210154249886	0.0015493373110342734	0.0	0.0	0.0
88	1.4433412687897218	0.0015493373110342734	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2790	0.0	88.09431	1
AACGTGT	1295	0.0	73.081566	1
ATCGAGT	910	0.0	72.43829	1
CGTGTGG	1445	0.0	69.30526	3
GTGGAAT	49925	0.0	68.416695	6
TGGAATC	50405	0.0	67.69655	7
ACGAGTG	46420	0.0	67.245544	2
AACGAGT	42645	0.0	67.21839	1
GAATCAC	50860	0.0	67.11507	9
GGAATCA	50865	0.0	67.10847	8
CGAGTGG	48220	0.0	66.80072	3
ACGTGTG	1485	0.0	66.17376	2
TCGAGTG	1080	0.0	66.17376	2
GAGTGGA	50065	0.0	66.108604	4
AGTGGAA	50095	0.0	66.097176	5
GCGAGTG	590	0.0	56.58122	2
AGCGAGT	615	0.0	52.82695	1
ATGAGTG	1885	0.0	49.88668	2
TGAGTGG	2040	0.0	49.321693	3
AATGAGT	1770	0.0	49.21302	1
>>END_MODULE