##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132788_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3771409
Sequences flagged as poor quality	0
Sequence length	100
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.208657029773224	31.0	25.0	33.0	16.0	34.0
2	28.456123692763104	31.0	26.0	34.0	16.0	34.0
3	29.498172433697857	31.0	28.0	34.0	16.0	34.0
4	34.92022000265683	35.0	35.0	37.0	32.0	37.0
5	34.017076376494835	37.0	35.0	37.0	28.0	37.0
6	35.3637505240084	37.0	35.0	37.0	32.0	37.0
7	35.722546931398846	37.0	35.0	37.0	35.0	37.0
8	35.980592399286316	37.0	35.0	37.0	35.0	37.0
9	37.94476520578914	39.0	38.0	39.0	35.0	39.0
10-11	37.930052534742316	39.0	38.0	39.0	35.0	39.0
12-13	37.86892829709002	39.0	38.0	39.0	35.0	39.0
14-15	39.26205325383696	41.0	39.0	41.0	36.0	41.0
16-17	39.013805583006246	40.5	39.0	41.0	36.0	41.0
18-19	38.31697728885942	40.0	37.0	41.0	34.5	41.0
20-21	37.96219967126345	40.0	36.0	41.0	34.0	41.0
22-23	37.64463665966751	40.0	35.5	41.0	34.0	41.0
24-25	37.19764735142754	40.0	35.0	41.0	33.0	41.0
26-27	36.84041786504726	40.0	35.0	41.0	33.0	41.0
28-29	36.683910045290766	40.0	35.0	41.0	32.5	41.0
30-31	36.53295227327506	40.0	35.0	41.0	31.5	41.0
32-33	36.38691229193121	40.0	35.0	41.0	31.0	41.0
34-35	36.2660416836254	40.0	35.0	41.0	31.0	41.0
36-37	36.01041878512778	39.5	35.0	41.0	30.5	41.0
38-39	35.45821641195638	38.0	35.0	41.0	30.0	41.0
40-41	35.18644450920067	38.0	35.0	41.0	30.0	41.0
42-43	34.98664557463802	38.0	35.0	41.0	28.0	41.0
44-45	34.84589313967273	37.5	35.0	40.0	27.0	41.0
46-47	34.684648628669024	37.0	35.0	40.0	26.5	41.0
48-49	34.49369227787281	37.0	35.0	40.0	25.5	41.0
50-51	34.18449749152107	36.5	35.0	40.0	24.0	41.0
52-53	33.62198252695478	36.0	34.5	40.0	19.5	41.0
54-55	32.65729956628941	35.0	33.0	40.0	4.0	41.0
56-57	31.946815102790495	35.0	33.0	40.0	2.0	41.0
58-59	31.607919347914798	35.0	33.0	40.0	2.0	41.0
60-61	31.359682946081954	35.0	33.0	39.5	2.0	41.0
62-63	31.127678806515018	35.0	33.0	39.0	2.0	41.0
64-65	30.865709738720994	35.0	33.0	39.0	2.0	41.0
66-67	30.541289343054544	35.0	32.5	38.5	2.0	40.5
68-69	30.28310957522772	35.0	32.0	37.5	2.0	40.0
70-71	30.033059660195963	35.0	32.0	37.0	2.0	40.0
72-73	29.798267570555197	35.0	31.5	36.5	2.0	39.5
74-75	29.547894434149146	35.0	31.0	36.0	2.0	39.0
76-77	29.28195483438683	35.0	31.0	35.5	2.0	39.0
78-79	29.00371426169901	35.0	31.0	35.0	2.0	38.5
80-81	28.729807082710998	35.0	30.5	35.0	2.0	37.0
82-83	28.440075446603643	35.0	30.0	35.0	2.0	37.0
84-85	28.175501384230667	35.0	29.0	35.0	2.0	36.0
86-87	28.019209398927565	35.0	29.0	35.0	2.0	36.0
88-89	27.790547776706266	35.0	29.0	35.0	2.0	36.0
90-91	27.591562066060725	34.5	29.0	35.0	2.0	35.0
92-93	27.454607415955152	34.0	29.0	35.0	2.0	35.0
94-95	27.243627646855593	34.0	28.5	35.0	2.0	35.0
96-97	26.985477708729018	34.0	27.0	35.0	2.0	35.0
98-99	26.814177937211262	34.0	27.0	35.0	2.0	35.0
100	26.733864982556916	34.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.244043716712131
1101	2	2.258269871937177
1101	3	1.196688290530087
1101	4	0.6129160643759235
1101	5	2.1353210195120056
1101	6	0.9158574526630048
1101	7	0.5174570674638801
1101	8	0.3424573761861893
1101	9	0.3149523302480972
1101	10-11	0.4181573863916128
1101	12-13	0.37295406703095324
1101	14-15	0.4796433636518671
1101	16-17	0.43700732521818964
1101	18-19	0.37965406171431937
1101	20-21	0.3776392615686177
1101	22-23	0.3089409441264621
1101	24-25	0.176204028858308
1101	26-27	0.07231777916793192
1101	28-29	0.13914167306133862
1101	30-31	0.19253688067225028
1101	32-33	0.2412221280811906
1101	34-35	0.24982688072898895
1101	36-37	0.21243573082654166
1101	38-39	0.22063852439434584
1101	40-41	0.213168685296651
1101	42-43	0.3084306995389028
1101	44-45	0.29725127267745677
1101	46-47	0.288969234235708
1101	48-49	0.3025661759654312
1101	50-51	0.28572405106025656
1101	52-53	0.3910272136434898
1101	54-55	0.31015703690776064
1101	56-57	0.24490487918822623
1101	58-59	0.17087216150944684
1101	60-61	0.22940161246764745
1101	62-63	0.2773499469201006
1101	64-65	0.2554275612826089
1101	66-67	0.25304005819100794
1101	68-69	0.3310307200622411
1101	70-71	0.28667502013748347
1101	72-73	0.2060822354667664
1101	74-75	0.21010735395538305
1101	76-77	0.2079938028785655
1101	78-79	0.28223277785694734
1101	80-81	0.3005885684034162
1101	82-83	0.3135508953295094
1101	84-85	0.28283551101706905
1101	86-87	0.32479842771279976
1101	88-89	0.23916616716125105
1101	90-91	0.2914141764528182
1101	92-93	0.3317245301856957
1101	94-95	0.40815807470768206
1101	96-97	0.37565525222523277
1101	98-99	0.3465449714398794
1101	100	0.3848486134389617
1102	1	1.5009789610767115
1102	2	2.9083461548922074
1102	3	3.0417397874740075
1102	4	1.2886694279650825
1102	5	2.4794958574008064
1102	6	1.092812041433099
1102	7	0.6265718890437313
1102	8	0.45181630322213806
1102	9	0.3684841914041357
1102	10-11	0.4467196153028823
1102	12-13	0.3772630756645441
1102	14-15	0.4553591939118533
1102	16-17	0.350827853239025
1102	18-19	0.14783324178228696
1102	20-21	0.057656564711003
1102	22-23	-0.14561987758241912
1102	24-25	-0.19593875802641492
1102	26-27	-0.10870314060619535
1102	28-29	-0.05098287043078642
1102	30-31	-0.024850833990896604
1102	32-33	-0.005245349731971771
1102	34-35	0.02231764745068432
1102	36-37	-0.017927674825330087
1102	38-39	-0.06711479583118773
1102	40-41	-0.0023885954024152056
1102	42-43	0.046201946919893544
1102	44-45	-0.020157528868409713
1102	46-47	0.05280597073191018
1102	48-49	0.019693603138343008
1102	50-51	0.10831780638154953
1102	52-53	0.076280224506597
1102	54-55	0.03764230120903278
1102	56-57	-0.07879230841654916
1102	58-59	-0.012814391431717809
1102	60-61	-5.96957061684833E-4
1102	62-63	0.03060787691006439
1102	64-65	0.06801184271039062
1102	66-67	0.09205756017145461
1102	68-69	0.10699282395936294
1102	70-71	0.01294090735187936
1102	72-73	0.05531381359584486
1102	74-75	0.08852998638100473
1102	76-77	0.11976986838090298
1102	78-79	0.13992758627964363
1102	80-81	0.07072084331185025
1102	82-83	0.11439895819195556
1102	84-85	0.14069742802756124
1102	86-87	0.12268022059756944
1102	88-89	0.12107274923007338
1102	90-91	0.1620330890201238
1102	92-93	0.22019370711244335
1102	94-95	0.26330782198238367
1102	96-97	0.3077325256688255
1102	98-99	0.3648506860247558
1102	100	0.30759071729377396
1103	1	1.2853645377930754
1103	2	2.838475035846294
1103	3	2.9831053203381614
1103	4	1.277554264101859
1103	5	2.4142903897427175
1103	6	1.0969105939389792
1103	7	0.6183318391802786
1103	8	0.41157914246971217
1103	9	0.29897694362264815
1103	10-11	0.34550307220362697
1103	12-13	0.2999599324416522
1103	14-15	0.3116210482117694
1103	16-17	0.09312625087535764
1103	18-19	-0.00682907145529299
1103	20-21	-0.09311545019647838
1103	22-23	-0.2144050151580359
1103	24-25	-0.10288751084708991
1103	26-27	0.021215372439911562
1103	28-29	0.06735014756119995
1103	30-31	0.08195856716815086
1103	32-33	0.08824159694062672
1103	34-35	0.11362774639912487
1103	36-37	0.04731953904364161
1103	38-39	0.08270525338268442
1103	40-41	0.14094653632176346
1103	42-43	0.14430988594335759
1103	44-45	0.20404627718995982
1103	46-47	0.26243161071587906
1103	48-49	0.2892406122846225
1103	50-51	0.27793215852005915
1103	52-53	0.2763696199121668
1103	54-55	0.23783302687391483
1103	56-57	0.20024879955031594
1103	58-59	0.26994724608177023
1103	60-61	0.3078104385263849
1103	62-63	0.27835409706712966
1103	64-65	0.3105733097848855
1103	66-67	0.30382513263251454
1103	68-69	0.286930038379964
1103	70-71	0.31987518626245404
1103	72-73	0.3134869071254016
1103	74-75	0.3459592781724581
1103	76-77	0.28362739355106825
1103	78-79	0.2428742707360314
1103	80-81	0.2616221412980799
1103	82-83	0.26323711435757957
1103	84-85	0.3164483313903048
1103	86-87	0.31190385965047085
1103	88-89	0.2736765450806615
1103	90-91	0.28438294572454836
1103	92-93	0.34822224713699157
1103	94-95	0.3775806181974666
1103	96-97	0.39923295073521103
1103	98-99	0.37509084209036914
1103	100	0.4002623819919222
1104	1	5.764983799500978
1104	2	4.832331855935813
1104	3	3.8655672611545953
1104	4	1.697458471759468
1104	5	2.6683177925718553
1104	6	1.2102521843883238
1104	7	0.6263498581489912
1104	8	0.4710717504655193
1104	9	0.38405222861840116
1104	10-11	0.41931059664976544
1104	12-13	0.2662261805947992
1104	14-15	0.3106690076994667
1104	16-17	0.10308342396916004
1104	18-19	-0.05109976892402557
1104	20-21	-0.14949291348696647
1104	22-23	-0.2248013848344783
1104	24-25	-0.15731042236662063
1104	26-27	-0.028983196784693632
1104	28-29	-0.09519381277939942
1104	30-31	0.0024887279634668857
1104	32-33	0.02525763130196168
1104	34-35	0.04692577322224878
1104	36-37	0.05802410819704562
1104	38-39	0.08617922857504823
1104	40-41	0.09464707264802996
1104	42-43	0.09830138723182813
1104	44-45	0.11964229996694087
1104	46-47	0.08475326849966791
1104	48-49	0.12202729426032732
1104	50-51	0.15638077505944636
1104	52-53	0.16668428615383846
1104	54-55	0.2722316636163953
1104	56-57	0.25791499278970775
1104	58-59	0.28682617260474785
1104	60-61	0.24561553751660625
1104	62-63	0.26766973347143264
1104	64-65	0.1879087098135095
1104	66-67	0.16007324090412212
1104	68-69	0.1397549380770613
1104	70-71	0.16539633777065532
1104	72-73	0.13044000123611
1104	74-75	0.1882050212473132
1104	76-77	0.18787043245906077
1104	78-79	0.198394638677879
1104	80-81	0.2706721230674667
1104	82-83	0.27275089935584873
1104	84-85	0.2676942314743016
1104	86-87	0.3011364424597289
1104	88-89	0.30234633296150903
1104	90-91	0.3337026286647067
1104	92-93	0.3341887291371073
1104	94-95	0.3542378856841317
1104	96-97	0.32038339379785086
1104	98-99	0.3095135874287429
1104	100	0.29322083265885013
1105	1	1.5290181988729792
1105	2	2.946594319761047
1105	3	3.0685573099485737
1105	4	1.2733056029122594
1105	5	2.409607093508548
1105	6	1.0543726983429877
1105	7	0.6287900743314836
1105	8	0.40502337193881743
1105	9	0.2832826654479277
1105	10-11	0.33220132144028014
1105	12-13	0.28281736365451593
1105	14-15	0.32958790958968365
1105	16-17	0.16109230596936897
1105	18-19	-0.0134759217363154
1105	20-21	-0.11066169171546392
1105	22-23	-0.16538718774080508
1105	24-25	-0.15497927667151146
1105	26-27	-0.07178745324392821
1105	28-29	-0.051351625161373704
1105	30-31	-0.03811822290595046
1105	32-33	-0.05988440718612509
1105	34-35	-0.05479627794932895
1105	36-37	-0.011400537587192616
1105	38-39	-0.003549734329951093
1105	40-41	-7.843853582869542E-4
1105	42-43	0.04468242701759095
1105	44-45	0.07812629209635702
1105	46-47	0.14457331458029898
1105	48-49	0.05286646895493874
1105	50-51	0.02795030622661443
1105	52-53	0.11084175974515631
1105	54-55	0.20833408151347754
1105	56-57	0.13445168605677793
1105	58-59	0.1922489778380445
1105	60-61	0.20727537943209384
1105	62-63	0.21503128940360128
1105	64-65	0.26500919565634007
1105	66-67	0.27606903689876106
1105	68-69	0.2906000834519915
1105	70-71	0.22421144678547122
1105	72-73	0.1825154775805089
1105	74-75	0.19975674137583255
1105	76-77	0.16900700970331783
1105	78-79	0.17306824545480737
1105	80-81	0.1538389817227177
1105	82-83	0.16606376625130537
1105	84-85	0.15929217507657256
1105	86-87	0.3082276473912593
1105	88-89	0.2414790130069946
1105	90-91	0.2003906332750276
1105	92-93	0.26408362828160037
1105	94-95	0.2816020994203505
1105	96-97	0.27627961015695135
1105	98-99	0.28750935307458647
1105	100	0.349113475459486
1106	1	-0.8859616306001143
1106	2	1.6953632234319151
1106	3	2.5149461559290707
1106	4	1.0382153247945496
1106	5	2.285549461952151
1106	6	1.0058335849902846
1106	7	0.5563453032947407
1106	8	0.37257700637083957
1106	9	0.2598758361449427
1106	10-11	0.3529775650354168
1106	12-13	0.30233003651386525
1106	14-15	0.28165866534960315
1106	16-17	0.19356677368465114
1106	18-19	0.03252096707142016
1106	20-21	0.0021584761343547143
1106	22-23	-0.15524552458415286
1106	24-25	-0.20164667221090582
1106	26-27	-0.27156705961944283
1106	28-29	-0.24749058064375618
1106	30-31	-0.19348804618753945
1106	32-33	-0.2475262186087761
1106	34-35	-0.20623242347101467
1106	36-37	-0.1385258708681576
1106	38-39	-0.09065684041894428
1106	40-41	-0.1065319474206774
1106	42-43	-0.04682483293584738
1106	44-45	6.322454526070942E-4
1106	46-47	-0.03009643084087088
1106	48-49	0.021468688421258264
1106	50-51	0.06449241626236812
1106	52-53	0.09626700422284529
1106	54-55	0.12231204073431456
1106	56-57	0.0682617006899271
1106	58-59	0.12074994603701228
1106	60-61	0.10770859216923512
1106	62-63	0.1883627179951297
1106	64-65	0.2502641784931434
1106	66-67	0.2429314391128159
1106	68-69	0.14976777821025422
1106	70-71	0.12494774991367308
1106	72-73	0.18140213914116288
1106	74-75	0.1852135809231008
1106	76-77	0.2002082950400883
1106	78-79	0.2359168232199096
1106	80-81	0.18128668241748258
1106	82-83	0.16608278191552373
1106	84-85	0.09434989573912844
1106	86-87	0.11712367828842929
1106	88-89	0.16094148304724598
1106	90-91	0.17750126941384892
1106	92-93	0.1591382292516208
1106	94-95	0.15141639222376568
1106	96-97	0.1832566662009114
1106	98-99	0.12867765301216494
1106	100	0.10383358588858727
1107	1	-0.9436460841096093
1107	2	1.6624007952892441
1107	3	-0.7271458741490022
1107	4	-0.09698527009174285
1107	5	1.7667774995535268
1107	6	0.7096498018104427
1107	7	0.30902690278392697
1107	8	0.25078137056569005
1107	9	0.25662999444078594
1107	10-11	0.31714176296685537
1107	12-13	0.25905995580775
1107	14-15	0.34232582285262225
1107	16-17	0.2927107999377654
1107	18-19	0.24538498742958836
1107	20-21	0.18179278161165513
1107	22-23	0.1594267594317671
1107	24-25	0.008994477910761134
1107	26-27	-0.06401361580401499
1107	28-29	-0.05905534515108002
1107	30-31	-0.02238899253348592
1107	32-33	0.00253117840010475
1107	34-35	0.0029446305733742406
1107	36-37	0.06419082811751764
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2216	94-95	0.08012585561721508
2216	96-97	0.03222416105634096
2216	98-99	-0.056133576632891646
2216	100	-0.1533450362595623
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1705.0
3	556.0
4	1069.0
5	4213.0
6	14466.0
7	26520.0
8	31619.0
9	43583.0
10	27434.0
11	11417.0
12	10820.0
13	13704.0
14	14983.0
15	16737.0
16	18820.0
17	20838.0
18	27680.0
19	45441.0
20	88855.0
21	102178.0
22	42168.0
23	26254.0
24	26177.0
25	28202.0
26	31417.0
27	35389.0
28	43461.0
29	49800.0
30	56047.0
31	69604.0
32	91073.0
33	139265.0
34	204287.0
35	585389.0
36	485436.0
37	650581.0
38	535106.0
39	148265.0
40	850.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.2824687609278	48.69238120045234	23.416725147409974	16.608424891209886
2	12.192931399546827	48.90712070609106	23.117242988609682	15.782704905752437
3	15.076149454241916	29.94735457676304	18.32534839740904	36.651147571586
4	32.93692092265782	31.748240511702658	20.017028118668645	15.29781044697088
5	14.118004867792939	47.790733162588474	20.956779312489875	17.134482657128714
6	36.47371807077215	28.564848135122535	21.963086972456043	12.998346821649264
7	15.714720578517918	28.620483378110208	41.072210796724306	14.592585246647568
8	35.7253747869828	28.903043928674933	21.252481499619904	14.119099784722366
9	34.85729073669815	30.385831926476286	21.917829649343258	12.839047687482319
10-11	13.310595589075595	51.136206653799675	21.604485220245273	13.948712536879453
12-13	13.041133963460341	29.959704715134315	32.037734438243106	24.961426883162236
14-15	13.355658322923873	40.14921213795693	21.579348726165737	24.91578081295346
16-17	24.286506714068935	29.264924594495056	32.183886711836344	14.264681979599667
18-19	13.71788368750247	29.620149922747707	32.47296965139554	24.188996738354287
20-21	24.327154652279827	40.38595389680621	21.92334482947885	13.363546621435118
22-23	23.655906850728734	29.90003205698454	32.56469929408346	13.879361798203272
24-25	13.532369467220343	40.49784841686489	21.255106513242133	24.714675602672635
26-27	14.591049284511248	34.55863168344555	36.33688001934556	14.513439012697646
28-29	24.013783707892728	30.033470249447884	31.65513207398084	14.297613968678549
30-31	20.560114270289965	32.12713073548905	32.01754304558323	15.295211948637762
32-33	16.149534563872546	33.721561358102505	32.84902273924679	17.27988133877816
34-35	16.69207715206704	33.88413985330151	32.82335593938499	16.60042705524646
36-37	17.32246489309433	33.00665613302615	33.56591661100666	16.104962362872868
38-39	18.75072419883391	32.06794861018786	32.792213732321265	16.389113458656965
40-41	15.623749638397744	35.15222029750685	32.86909481310566	16.35493525098975
42-43	15.354205177653116	32.91566576217913	34.45388233831086	17.276246721856893
44-45	15.901285169548038	34.21186882674353	32.376825213070234	17.510020790638194
46-47	17.582858289832792	32.24240595490969	33.649280679979285	16.52545507527823
48-49	15.999643634514316	32.84226134052287	33.55517791891571	17.6029171060471
50-51	16.97500854455192	34.53216026158923	31.91909177710506	16.573739416753792
52-53	17.474394151355835	33.007813535754856	32.70088539970791	16.816906913181402
54-55	17.116639961351314	34.5710714483632	29.786241693754246	18.52604689653124
56-57	17.526526558111303	35.47867653707142	28.848448948390377	18.146347956426894
58-59	19.57740462516794	35.0107877453758	27.13028472912909	18.28152290032717
60-61	18.83997466198972	35.81262334581054	26.67378425410768	18.673617738092048
62-63	17.216901117155864	35.815222831263924	26.25579442157868	20.71208163000153
64-65	18.401676932944692	35.82368022137084	27.576656363709162	18.197986481975303
66-67	18.914031864483537	34.51581358585081	28.2992245073393	18.270930042326356
68-69	19.64438224546847	34.900563688531264	27.47687933077532	17.978174735224954
70-71	16.15658763077672	37.41826993571898	27.972264477281566	18.45287795622273
72-73	16.388600092432544	35.537860088524184	28.657150834478134	19.416388984565145
74-75	16.945902163348688	36.443192094391506	27.01150514855431	19.59940059370549
76-77	18.94387564488771	34.509145559744105	27.675118981235848	18.87185981413234
78-79	18.180873270452132	35.11291238857744	26.928041926102992	19.778172414867434
80-81	19.109211422086492	36.556682538409376	26.118374519654953	18.21573151984918
82-83	18.54867556051927	36.381046246071385	26.43175462279082	18.63852357061852
84-85	18.373857327310937	36.91916444609666	25.23385091319022	19.473127313402188
86-87	18.14580075817858	36.96019814355756	26.266258189582825	18.62774290868103
88-89	20.916890742955747	35.289304342223296	25.774557996759302	18.019246918061658
90-91	18.79958031567007	36.76685830165196	25.956950802963142	18.476610579714833
92-93	17.35262603446086	37.037589929917445	26.24265891076783	19.367125124853867
94-95	18.106497877384538	37.257618738463364	25.89003551325718	18.74584787089492
96-97	18.196042598911163	35.46913000177124	26.828927146782966	19.505900252534627
98-99	19.86915683904496	35.45980648225964	25.250892543337187	19.420144135358214
100	17.561526739741034	37.57073284812122	25.02091393428822	19.846826477849525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	452021.0
1	243010.0
2	23577.5
3	10489.5
4	7338.0
5	6526.0
6	6599.0
7	7770.5
8	7478.5
9	6047.5
10	5655.0
11	8999.5
12	63889.5
13	67438.5
14	15003.5
15	10347.5
16	10561.0
17	11079.5
18	11475.5
19	12332.0
20	13436.0
21	14707.0
22	16616.0
23	19425.5
24	22431.0
25	25467.5
26	28656.5
27	31904.5
28	35113.5
29	39190.0
30	44195.5
31	48668.0
32	53604.0
33	59306.0
34	65192.0
35	71287.5
36	76903.5
37	80165.0
38	81152.5
39	80351.0
40	77942.0
41	75107.0
42	73349.5
43	73304.0
44	75684.0
45	79524.5
46	84888.5
47	95949.0
48	104281.0
49	121191.0
50	188232.5
51	197572.5
52	129314.0
53	101888.0
54	101644.0
55	109405.0
56	99040.0
57	74107.0
58	57169.0
59	42033.5
60	32750.5
61	27132.5
62	21698.5
63	15480.5
64	10481.5
65	7024.5
66	5473.5
67	4614.0
68	3498.5
69	2733.0
70	2370.5
71	1942.0
72	1452.0
73	1122.5
74	819.0
75	576.5
76	406.5
77	265.5
78	185.5
79	124.0
80	72.0
81	53.5
82	31.0
83	14.5
84	15.5
85	17.0
86	9.0
87	5.5
88	4.0
89	1.5
90	2.5
91	3.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13819768685920833
2	0.0059394247614088
3	0.003738655764993932
4	0.0
5	0.0023863760202088927
6	0.013019006954695184
7	0.0031022888262715606
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	2.6515289113432138E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.55685739732816E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.3257644556716072E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	3.977293367014821E-5
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	2.6515289113432144E-4
74-75	2.784105356910375E-4
76-77	4.242446258149142E-4
78-79	3.049258248044696E-4
80-81	8.352316070731123E-4
82-83	0.0010606115645372858
84-85	0.001126899787320866
86-87	5.3030578226864277E-5
88-89	0.0
90-91	7.954586734029642E-5
92-93	0.0
94-95	1.3257644556716069E-5
96-97	0.0011931880101044463
98-99	6.231092941656553E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3771409.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.97487179997556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.117527274511	30.835954826551365
2	12.18602745111831	9.498977153166383
3	4.329617754602121	5.062388907855472
4	1.8036505816390886	2.8118820076533932
5	0.8302686508700249	1.6179807113598943
6	0.4141716757024168	0.9685372778209651
7	0.24738989187912952	0.6749392524419222
8	0.16401618395481232	0.5114007794208014
9	0.11219754826903	0.3935596554051313
>10	0.5742847117874867	4.3020121849674
>50	0.08824693848082929	2.4669059359920875
>100	0.11875869398007219	8.622667866365651
>500	0.006887226422568622	1.8069242576659386
>1k	0.0058643710132639925	5.110697681588287
>5k	4.773325243354412E-4	1.4946698436004915
>10k+	6.137132455741387E-4	23.820501658144792
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	413200	10.95611746167016	No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	311214	8.25192918614767	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	61207	1.6229213007658412	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32554	0.86317872179867	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24743	0.6560677985336515	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	19611	0.5199913348035178	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	12484	0.33101686929208685	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10782	0.28588784722102534	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10484	0.27798629106522255	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9853	0.26125514363464686	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8722	0.23126635164735515	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8584	0.22760724174970148	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8427	0.22344434135889266	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7409	0.19645177704141875	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7206	0.191069173351392	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6038	0.16009931566690327	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	4835	0.1282014228634444	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4767	0.126398383203731	No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA	4555	0.1207771419116834	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	4401	0.11669378738821486	No Hit
TACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	4328	0.11475817128293431	No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG	4190	0.11109906138528068	No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC	4071	0.10794374198078226	No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT	3984	0.10563691182791367	No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT	3861	0.10237553126696149	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0014053103230119036	0.0	0.0	0.0	0.0
2	0.0014053103230119036	0.0	0.0	0.0	2.651528911343214E-5
3	0.0014053103230119036	0.0	0.0	0.0	2.651528911343214E-5
4	0.0014053103230119036	0.0	0.0	0.0	2.651528911343214E-5
5	0.0014053103230119036	0.0	0.0	0.0	2.651528911343214E-5
6	0.0014053103230119036	0.0	0.0	0.0	2.651528911343214E-5
7	0.0014053103230119036	0.0	0.0	0.0	2.651528911343214E-5
8	0.0014053103230119036	0.0	0.0	0.0	2.651528911343214E-5
9	0.0016439479250327928	0.0	0.0	0.0	2.651528911343214E-5
10-11	0.0019223584607238303	0.0	0.0	0.0	7.954586734029642E-5
12-13	0.0023068301528685964	0.0	0.0	0.0	7.954586734029642E-5
14-15	0.0025587253994462014	0.0	0.0	0.0	7.954586734029642E-5
16-17	0.0027178171341267947	0.0	0.0	0.0	9.280351189701249E-5
18-19	0.002943197091590968	0.0	0.0	0.0	1.3257644556716072E-4
20-21	0.003062515892601412	0.0	0.0	0.0	1.3257644556716072E-4
22-23	0.003195092338168573	0.0	0.0	0.0	1.3257644556716072E-4
24-25	0.003354184072849166	0.0	0.0	0.0	1.85607023794025E-4
26-27	0.003513275807529759	0.0	0.0	0.0	1.85607023794025E-4
28-29	0.0037651710541073643	0.0	0.0	0.0	1.85607023794025E-4
30-31	0.00401706630068497	0.0	0.0	0.0	1.85607023794025E-4
32-33	0.0043219921254894385	0.0	0.0	0.0	1.85607023794025E-4
34-35	0.004693206173077489	0.0	0.0	0.0	1.85607023794025E-4
36-37	0.005607983647490898	0.0	0.0	0.0	1.85607023794025E-4
38-39	0.007013293970502802	0.0	0.0	0.0	1.85607023794025E-4
40-41	0.009996263995763918	0.0	0.0	0.0	1.85607023794025E-4
42-43	0.013045522243808613	0.0	0.0	0.0	1.85607023794025E-4
44-45	0.01720842263461746	0.0	0.0	0.0	1.85607023794025E-4
46-47	0.0207879866649308	0.0	0.0	0.0	1.85607023794025E-4
48-49	0.024539900074481445	0.0	0.0	0.0	1.85607023794025E-4
50-51	0.03091682710626188	1.325764455671607E-5	0.0	0.0	1.85607023794025E-4
52-53	0.039189597309652706	2.651528911343214E-5	0.0	0.0	1.85607023794025E-4
54-55	0.049132830727189755	2.651528911343214E-5	0.0	0.0	1.85607023794025E-4
56-57	0.058360151338664144	2.651528911343214E-5	0.0	0.0	1.85607023794025E-4
58-59	0.06775982132937583	2.651528911343214E-5	0.0	0.0	2.253799574641732E-4
60-61	0.08213110802885606	2.651528911343214E-5	0.0	1.325764455671607E-5	2.5189524657760537E-4
62-63	0.10089067507660929	2.651528911343214E-5	0.0	2.651528911343214E-5	2.6515289113432144E-4
64-65	0.12215593694558188	2.651528911343214E-5	0.0	2.651528911343214E-5	2.6515289113432144E-4
66-67	0.14531704198616485	2.651528911343214E-5	0.0	2.651528911343214E-5	2.9166818024775354E-4
68-69	0.17582288211116853	2.651528911343214E-5	0.0	2.651528911343214E-5	2.9166818024775354E-4
70-71	0.2204215983999614	2.651528911343214E-5	0.0	2.651528911343214E-5	2.9166818024775354E-4
72-73	0.2651528911343214	2.651528911343214E-5	0.0	2.651528911343214E-5	2.9166818024775354E-4
74-75	0.3136493549227888	2.651528911343214E-5	0.0	2.651528911343214E-5	2.9166818024775354E-4
76-77	0.3634583255223711	2.651528911343214E-5	0.0	2.651528911343214E-5	3.314411139179018E-4
78-79	0.4196442231537338	2.651528911343214E-5	0.0	2.651528911343214E-5	3.5795640303133393E-4
80-81	0.49183209776505277	2.651528911343214E-5	0.0	2.651528911343214E-5	3.7121404758805E-4
82-83	0.5824746135993206	2.651528911343214E-5	0.0	2.651528911343214E-5	3.7121404758805E-4
84-85	0.694236557212437	2.651528911343214E-5	0.0	2.651528911343214E-5	3.977293367014821E-4
86-87	0.8121102749661997	2.651528911343214E-5	0.0	2.651528911343214E-5	3.977293367014821E-4
88	0.9070350099922867	2.651528911343214E-5	0.0	5.303057822686428E-5	3.977293367014821E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	6195	0.0	85.795074	1
AACGTGT	2565	0.0	80.20543	1
CGTGTGG	3035	0.0	74.80483	3
ACGTGTG	2930	0.0	74.28	2
TCGAGTG	2210	0.0	72.53049	2
ATCGAGT	2115	0.0	72.11811	1
ATGAGTG	3155	0.0	62.874073	2
AGCGAGT	1010	0.0	62.45873	1
TGAGTGG	3380	0.0	61.745808	3
AATGAGT	3050	0.0	60.968704	1
GTGTGGA	4120	0.0	57.613453	4
GTGGAAT	130015	0.0	57.540047	6
TGGAATC	130645	0.0	57.26521	7
GGAATCA	132820	0.0	56.322426	8
GAATCAC	133765	0.0	55.935074	9
AGTGGAA	132515	0.0	54.727104	5
GAGTGGA	135420	0.0	53.60517	4
CGAGTGG	132235	0.0	53.490124	3
ACGAGTG	128950	0.0	53.24008	2
AACGAGT	121320	0.0	52.17984	1
>>END_MODULE