FastQCFastQC Report
Thu 26 May 2016
SRR2132787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132787_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1376513
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1187848.629340950648487No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA996517.239379504588769No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG116050.8430723138829782No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT93920.6823037632045611No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79890.5803795532624828No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38600.28041871017563946No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC38010.27613251745533823No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32420.2355226576138402No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27620.20065193717749125No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26640.19353249842173667No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23770.1726827134941697No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23050.16745210542871736No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20200.14674761516963516No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19190.13941023441115338No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA17340.12597047757631058No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15360.11158630539631663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT23100.087.8525161
AACGTGT9650.074.6539841
ACGTGTG10200.073.747182
CGTGTGG10450.073.327253
GTGGAAT401500.070.574896
TGGAATC405450.069.807617
AACGAGT348750.069.3560261
ACGAGTG380750.069.023582
GGAATCA410350.068.9944468
GAATCAC410400.068.986049
AGTGGAA400900.068.584165
CGAGTGG393600.068.4612963
GAGTGGA406250.067.773534
ATCGAGT8000.062.388531
TCGAGTG8450.059.53232
AGCGAGT4100.058.5699921
TGAGTGG12700.057.3749123
GCGAGTG4150.056.6431662
GTGTGGA15400.053.4189834
ATGAGTG13350.050.359382
Produced by FastQC (version 0.11.5)